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      • KCI등재

        수두생바이러스백신 국가표준품 (2차) 제조 및 확립에 관한 연구

        김연희(Yeon Hee Kim),김도근(Dokeun Kim),손여원(Yeo Won Sohn),한의리(Euiri Han),김석환(Seok Hwan Kim),임종미(Jong-Mi Lim),원윤정(Yun Jung Won),윤희성(Heui Seong Yoon),조문희(Moon Hee Jo),김관수(Kwan Soo Kim),김재욱(Jaeok Kim) 한국생물공학회 2010 KSBB Journal Vol.25 No.6

        수두 생바이러스 백신과 같은 생물의약품은 다양한 물질이 복합적으로 구성되어 있어 단순한 물리·화학적 분석방법만으로는 그 특성을 규명할 수 없다. 따라서 이러한 생물의약품의 품질을 평가하기 위해서는 표준품이 필수적이다. 2002년과 2003년에 제조 및 확립한 1차 국가표준품의 재고량 소진 및 역가 감소에 따라 식품의약품안전평가원에서는 수두 생바이러스 백신의 2차 국가표준품을 확립하기 위하여 2008년 용역연구사업을 통해 국내의 수두 생바이러스 백신 제조회사에서 표준품 후보물질을 제조하였으며, 국가표준품후보물질의 역가산정을 위하여 국내 제조사 및 식품의약품안전평가원에서 공동연구를 수행하였다. 국내제조사를 포함한 3개의 공동연구 시험소에서 7회 이상의 반복시험을 수행하여 얻은 공동연구 결과를 통계학적으로 분석한 결과 3곳의 공동연구 시험소의 통합역가에 대한 변이계수 (coefficient variation, CV)는 1.24%로 각 시험소간의 기하평균 (GMT) 변동 수준이 매우 낮음을 확인할 수 있었다. 또한, 수두 생바이러스 백신의 2차 국가표준품의 표시역가는 4.26 log10 PFU/0.5 mL로 산정하였다. Biological products, such as live varicella vaccine, are composed of biological substances derived from biological organisms. It is very difficult to identify these biologics’ characteristics by analysis of simple physical and chemical methods alone. So the reference material is essential in order to evaluate the quality of bilogics. The 1’st national standard for varicella live vaccine was manufactured, established in 2002 and 2003, and have been used for the manufacturer’s quality control and national lot release since then. As the lack of its availability and the decrease of its stability, this study was initiated by National Institute of Food and Drug Safety Evaluation (NiFDS) in 2008 to manufacture and establish the 2nd national standard for varicella live vaccine. The candidate material was manufactured from one of domestic manufacterers and the joint research of the NiFDS and manufacturers of varicella live vaccine was conducted to estimate of the reliable virus content. In the collaborative study, 3 laboratories including NiFDS performed the virus content test more than 7 times and all assay results were statistically analyzed. The mean coefficient of variation (CV) was 1.24%, and the geometric mean titre (GMT) variation range of each laboratory was low. On the basis of the results of this study, the candidate material of 2nd national standard for varicella live vaccine was assigned a potency of 4.26 log10 pfu/0.5 mL, when reconstituted in 0.7 mL.

      • 뇌기능 장애 환자의 가상영상(Moving Sorround) 자극에 따른 자세 균형 제어

        김연희 ( Yun Hee Kim ),최종덕 ( Jong Duk Choi ),이성범 ( Sung Bom Lee ),김종윤 ( Jong Yun Kim ),이석준 ( Suk Jun Lee ),박찬희 ( Chan Hee Park ),김남균 ( Nam Gyun Kim ) 한국감성과학회 2002 추계학술대회 Vol.2002 No.-

        뇌기능 장애 환자에서 자세균형 제어능력의 저하는 보행 및 일상생활동작 수행 등에 어려움을 초래하며 이에 대한 정확한 평가 및 치료를 위하여 일상의 환경변화와 유사한 상황을 제공하고 이예 따른 자세제어력을 파악하는 것이 중요하다. 본 연구는 뇌기능 장애환자에서 움직이는 환경에 따른 자세균형제어기능을 정확히 평가하는 환경의 움직임이 자세균형제어에 미치는 영향을 분석하였다. 15명의 뇌기능 장애 환자들과 정상인 15명을 대상으로 실생활과 유사한 환경의 조성을 위하여 HMD를 이용한 가상영상 환경변화(Moving Surround)를 네가지 움직임 종류에 따라 제공하였다. 자세동요의 정도는 힘판을 이용하여 신체압력중심의 변화를 전체이동거리, 동요주파수, 최대 빈도 COP 위치 등으로 측정하였으며 환경의 차이에 따른 변화를 비교 분석하였다. 연구결과 검사 재검사 신뢰도 평가에서 일관된 분석결과를 나타냈고 뇌기능장애 환자와 정상인간의 분석에서는 두 그룹간의 차이를 확인할 수 있었다. 특히 전후로 빠르게 변하는 가상영상 환경에서 가장 큰 자세동요를 나타내었고 통계적으로 유의한 차이가 있었다. 본 연구를 통해 뇌기능 장애 환자에서 가상영성 환경변화가 자세균형제어에 미치는 영향을 확인할 수 있었으며 이러한 환자들을 위한 치료환경 조성 등에도 유용한 자료로 쓰일 수 있을 것으로 사료된다.

      • KCI등재후보

        단보 : 어린이놀이터 바닥재의 유해물질 노출실태

        김연희 ( Yun Hee Kim ),김종민 ( Jong Min Kim ),송형명 ( Hyung Myung Song ),김승호 ( Seung Ho Kim ),안병용 ( Byeung Yong Ahn ),강영주 ( Yeong Ju Kang ),김은선 ( Eun Sun Kim ),김동수 ( Dong Soo Kim ) 한국환경분석학회 2013 환경분석과 독성보건 Vol.16 No.2

        This study was performed to perceive environmental pollution in children`s playground in Gwangju metropolitan city. Two types of sand and synthetic rubber as flooring material were used in children`s playground. The sampling was collected from 20 sites in sand flooring material and 21 sites in synthetic rubber flooring material. They are measured to evaluate pH, heavy metals, and parasite eggs in sites of sand flooring material and also to heavy metals in sites of synthetic rubber floor material. In site of sand flooring material, pH range was from 5.8 to 8.8. The average concentration of Pb, As, Hg, Cd, and Cr6+ was 16.318, 0.343, 0.048, 0.258, and 0.000 in unit mg/kg, respectively. In site of synthetic rubber flooring material. The average value of the total sum concentration of heavy metals such as Pb, As, Hg, Cd, and Cr6+ was 70.427 mg/kg. This total concentration of heavy metals was even much lower than that of criteria (1,000 mg/kg). The parasite eggs were not found in sites of sand flooring material. Therefore, it showed that all sites in children`s playground was safe environmentally.

      • KCI우수등재

        아동이 지각한 사회적 지지 및 귀인 성향과 아동의 자아존중감의 관계

        김연희(Yun Hee Kim),박경자(Kyung Ja Park) 한국아동학회 2001 아동학회지 Vol.22 No.2

        This study explored the relationship of children`s perceived social support and locus of control to their self-esteem. Subjects were 190 5th grade children. Instruments were the revised Coopersmith Self-Esteem Inventory (1967), Dubow and Ullman`s Social Support Appraisal Scale (1989), and Crandall`s Intellectual Achievement Responsibility (1965). Data were analysed by t-tests and Pearson`s correlations. Children with high perceived social support had higher self-esteem than children with low perceived social support. Children who attributed their success to internal factors had high self-esteem; children who attributed their success to external factors had low self-esteem. Successful children who attributed their success to external factors had low self-esteem, regardless of their social support level. Children who attributed their success to their abilities or hard work had high self-esteem only if they received high social support.

      • SCOPUSKCI등재

        Preference Analysis for Flower Jewelry Using Conjoint Analysis

        김연희(Yeon Hee Kim),김미진(Mi Jin Kim),윤숙영(Suk Young Yun),장태원(Taeg Won Chang),최병진(Byung Jin Choi) 인간식물환경학회 2022 인간식물환경학회지 Vol.25 No.3

        Background and objective: This study was conducted to determine the preferences in flower jewelry by conducting a surveyusing conjoint analysis on the perception of flower jewelry and provide basic data for development of flower jewelryproducts. Methods: For the conjoint analysis, four attributes of flower jewelry were selected. Attribute 1 was related to the mainmaterial of flower jewelry, presented in three levels: cut flowers, potted flowers, and processed flowers. Attribute 2 wasrelated to wearing area presented in five levels of wearing area: hair, neck, ear, arm (wrist) and chest (shoulder). Attribute3 was related to price provided for selling products in flower shops, presented in three levels: less than 30,000 won,50,000-70,000 won, and more than 100,000 won. Attribute 4 was related to flower jewelry wearing time, presented inthree levels: less than 6 hours, 12-24 hours, and more than 24 hours. After extracting 25 profiles through orthogonaldesign, a questionnaire survey was conducted on 402 general participants, from which the responses of 343 participantswere analyzed. Results: By attribute of flower jewelry, the respondents attached most importance to price (34.14%), followed by wearingarea (29.18%), wearing time (18.51%), and material (18.17%), proving that the general public attached most importanceto price (Pearson's R = .987, p = .000). The preference in levels by attribute was highest for processed flowers in material,less than 30,000 won in price, hair in wearing area, and 12-24 hours in wearing time. Conclusion: By making flower jewelry based on the results of the analysis, it would be possible to help increase items soldin flower shops as well as their income.

      • KCI등재
      • KCI등재

        광주지역 산업폐수 업종별 TOC 배출 특성에 관한 연구

        김연희 ( Yun-hee Kim ),문경란 ( Kyoung-ran Moon ),윤상훈 ( Sang-hoon Yoon ),김지혜 ( Ji-hye Kim ),임민화 ( Min-hwa Lim ),진재성 ( Jae-sung Jin ),양명승 ( Myeong-sueng Yang ),정희윤 ( Hee-yun Jung ),배석진 ( Seok-jin Bae ),정재근 ( 한국수처리학회 2021 한국수처리학회지 Vol.29 No.2

        In this study, 182 industrial wastewater samples obtained from Gwangju were assigned to 16 industries to investigate the characteristics of organic indexes(TOC, NPOC, COD<sub>Mn</sub>, and DOC). The average COD<sub>Mn</sub> and TOC concentrations by industrial wastewater classification in Gwangju in descending order were as follows : other machinery and equipment manufacturing > painting and other film treatment > textile dyeing, cleaning, and finishing processing. The correlations between TOC and COD<sub>Mn</sub>, NPOC, DOC showed high positive correlation coefficients which were more than 0.8. According to the revised Water Environment Conservation Act 2020 in which COD<sub>Mn</sub> from wastewater discharge facilities was replaced with TOC, a COD<sub>MN</sub> concentration of 130 mg/L in an “Na” region where the volume of wastewater discharge is lower than 2,000 m3/day, corresponds to 75 mg/L TOC, and the concentration ratio of COD<sub>MN</sub> to TOC is 1.73. The average ratio of COD<sub>Mn</sub> to TOC in five metropolitan cities (Gwang, Ulsan, Daejeon, Incheon, and Busan) was 1.55, and the converted TOC concentration corresponding to 130 mg/L COD<sub>Mn</sub> was 84 mg/L. The average measurement ratio of COD<sub>Mn</sub> to NPOC was 1.7, which was close to the COD<sub>Mn</sub> to TOC ratio of 1.73. These results propose that TOC concentration in wastewater discharge from “Na” regions should be changed to 84 mg/L from the current 75 mg/L or TOC concentrations might be applicable to NPOC concentrations in all wastewater discharge facilities.

      • KCI등재

        노인의 자아존중감과 삶의 질과의 관계 연구

        김연희(Yun Hee Kim),김기순,Kyung Sik Pyo 한국노인복지학회 2002 노인복지연구 Vol.17 No.-

        노인의 자아존중감과 삶의 질과의 관계를 규명하며 노년기의 적응을 돕기 위한 간호중재방법에 도움이 되는 자료를 제공하기 위하여 연구대상은 2001년 6월1일부터 30일까지 K 광역시의 60세 이상 노인들 142명을 대상으로 하였고, 수집된 자료는 SPSS /PC를 이용하여 일반적 특성에 따른 주요변수는 ANOVA, 자아존중감과 삶의 질과의 상관관계는 Pearson Correlation Coefficient, 일반적 특성에서 주요변수와의 다단계 분석은 Stepwise Multiple Regression을 이용하여 분석하였다. 본 연구결과를 요약하면 다음과 같다. 1. 본 연구의 가설인 "노인들의 자아존중감 정도가 높을수록 삶의 질 정도도 높을 것이다."는 지지되었다(p<0.05, r=0.407). 본 연구의 대상자들이 자아존중감 정도를 높게 지각한 사람들이 삶의 질 정도도 높게 지각한 것으로 나타났다. 2. 종속변수를 예측하기 위한 다단계 다중회귀분석에서는 삶의 질 변화에 영향을 미치는 요인으로 월수입, 운동종류, 자아존중감 등이 삶의 질 변화에 영향을 미치는 요인으로 규명되었으며 33%를 설명하였다. 분석결과 3개요인 중 가장 영향을 많이 미치는 요인은 자아존중감(B=0.381)이었고, 운동종류(B=-0.298)가 삶의 질에 가장 적게 영향을 미치는 변수로 나타났다. 이상의 결과들은 노인정의 노인들은 자아존중감을 높게 지각한 사람들이 삶의 질이 높은 것으로 나타났다. 노인을 돌보는데 관련된 봉사자들은 노인 자신이 긍정적인 자신의 생각을 갖도록 도와야 할 것이며, 노인의 자아존중감을 높게 지각하도륵 도와서 행복한 삶을 살수 있도록 노인을 지지하고 삶의 질을 높일 수 있도록 해야 할 것으로 사료된다. This study is designed to provide basic data which are helpful for nursing intervention to encourage the adjustment of the old to the changeable society through identifying a relationship of self-esteem and consequence about their quality of life. For this study, 142 persons were selected from three centers for the elderly located in B dong, G city and questionnaire which contained 68 questions were distributed to them for 30 days from June 1st to 30st, 2001. The questions were composed of 10 items about general features, 10 items for measuring self-esteem and 44 items about quality of the life. The results of this study are as follows: 1. As a result of analysing the questions about self-esteem it is shown that the question with the highest point was "I think I am as much valuable as others"(3.40) and the question with the lowest was "I sometimes think lam not good"(2.90). 2. As a result of analysing the questions related to quality of their life, it is shown that the question with the highest point was "What do you think of your children these days?"(3.59) and the question with lowest was "Do you think you are valuable these days?"(2.63). 3. As a result of analysing self-esteem according to the general characteristics, there was a statistically signifiant difference in educational background and monthly income (P<0.05). By the educational background, the old people with college degrees showed 3.52, the highest score and by the monthly income, those who make more than 2 million won showed 3.20 (±0.30), the highest score. 4. As a result of analysing the quality of the life according to general characteristics, there were statistically significant difference in educational background monthly income, possession of housing, kinds of sports and leisure(P<0.05). By the educational background, subjects with bachelors degrees or above showed 3.42, the highest score. Subjects who make 2 million won as monthly income showed 3.49, the highest score. Subjects who own their private houses and who enjoy golf showed 3.18 and 3.38 respectively 5. The assumption of this study that "the higher the self-esteem of the old, the more satisfactory their life." was supported (p<0.06, r=0.407). It is demonstrated that the people who have high self-esteem thought quality of their life as satisfactory, 6. By stepwise multiple regression to identify predictor of quality of life, monthly income, practice of sports and self-esteem were statistically significant variable. In conclusion, nursing services to encourage the old people to have positive attitude to their living and efforts to increase their monthly income and to practice sports regularly will help the elderly to feel the quality of their life as satisfaction.

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