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김소운,민철우,GUPTARAVI,조익현,방경환,김영창,김기홍,김선태 한국약용작물학회 2014 한국약용작물학회지 Vol.22 No.5
Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). Theobjective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomicsapproach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hoursafter exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and wereseparated on high resolution 2-DE. Approximately 1003 ± 240 (0 h), 992 ± 166 (6 h), 1051 ± 51 (12 h) and 990 ± 160 (18 h)spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identifiedby using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein(spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b bindingprotein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase(spot GL 8) were down-
An Unexpected Death Related to Accidental Gasoline Inhalation
김소운,이영만,이경홍,박종신,박재홍,최병하,박소형 대한법의학회 2016 대한법의학회지 Vol.40 No.3
Gasoline is a chemical compound of aliphatic and aromatic hydrocarbons, and it easily vaporizes because of its volatile characteristics. Gasoline inhalation may occur accidentally or intentionally in various situations which could result in acute non-lethal or lethal intoxication. Given that gasoline is widely used in everyday life, it is surprising that deaths related to gasoline inhalation rarely occur. We presented a rare case of unexpected death related to gasoline inhalation with the postmortem findings and features.
김소운,이성택 한국통신학회 2022 韓國通信學會論文誌 Vol.47 No.10
Deep Voice is a speech synthesis technology using AI that provides an opportunity for anyone to easily access speech synthesis and manipulation through online. This causes not only the production of altered content that synthesizes voice, but also the spread of cybercrime by misusing the content. A representative example of a similar crime is voice phishing, and there are appearing voice phishing crimes that misuse deep voice abroad as well as in Korea, so a service that can reduce damage should be developed. The victims are not only celebrities, public institutions, financial companies, etc., but also acquaintances or family members. In this study, we are going to propose a service (program) to prevent voice phishing damage that may occur in the course of telephony misusing deep voice technology. This service intend to develop this service for the purpose of distinguishing the actual speaker from the deep voice through the processes of deep learning, voice synthesis, speaker recognition, speaker identification, and speaker verification. It is expected that it will be linked to the expansion of services that can avoid the risk of various crimes as well as simple voice phishing. 딥보이스(Deep Voice)는 인공지능(AI)을 이용한 음성합성 기술로 온라인을 통하여 누구나 쉽게 음성합성 및 조작에 접근할 수 있는 기회를 제공하고 있어, 단순히 음성을 합성한 변조된 콘텐츠의 생산 뿐만 아니라, 해당 콘텐츠를 악용하여 사이버범죄 등이 확산되는 결과를 불러일으키고 있다. 유사범죄의 대표적인 사례가 보이스피싱이며, 해외에서는 물론 국내에서도 딥보이스를 악용한 보이스피싱 범죄가 나타나고 있어 이와 같은 피해를 줄일 수 있는 서비스가 개발되어야 한다. 피해의 대상자는 유명인, 실제 공공기관, 금융회사 등과 같이 전문직에서 근무하는특정인 뿐만 아니라, 가까운 지인 혹은 가족 등을 사칭하는 맞춤형 피싱도 등장하고 있다. 본 연구에서는 전화통신 과정에서 발생할 수 있는 딥보이스 기술을 악용한 보이스피싱 피해를 방지하기 위한 서비스(프로그램)을 제안하려 한다. 해당 서비스는 딥러닝, 음성합성, 화자인식, 화자식별, 화자검증 등의 과정을 통하여 실제 화자와 딥보이스를 구분하는 목적으로 개발하고자 하며, 이는 단순 보이스피싱뿐만 아니라 다양한 범죄들의 위험으로부터 벗어날 수 있는 서비스의 확장으로 연계될 것이라고 기대한다.
최신 비선형 유한요소해석 S/W들의 토목공학분야 적용사례
김소운 한국전산구조공학회 1993 전산구조공학 Vol.6 No.1
본 고에서는 현재 상용화되어 있는 비선형 유한요소해석 S/W들의 주요기능들을 살펴보고 이를 토목공학분야에 적용한 몇몇 대표적 사례들을 소개하고자 한다. 그 기본이론에 있어서는 매우 유사하나 각각의 적용목적에 따라 그 기능이 매우 다변화되어 발달되고 있다. 따라서 본 고에서는 토목공학분야에서 요구되는 기능들을 중심으로 하거나 이를 포함하여 개발되어 상용화되어 있는 DIANA, MARC, ABAQUS 등의 대형 비선형 전용 S/W들을 중심으로 알아보기도 한다.
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips
김소운,노진,박찬식 대한병리학회 2016 Journal of Pathology and Translational Medicine Vol.50 No.6
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication.