사람골수줄기세포가 연골조직으로 분화되는 과정에 나타나는 세포표지자의 표현

김상경 ( Sang Gyung Kim ),최정윤 ( Jung Yoon Choe ),김채기 ( Chae Gi Kim ),정승혜 ( Seung Hie Chung ),신임희 ( Im Hee Shin ),서헌석 ( Hun Suk Suh ) 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.1

Objective: Multipotent bone marrow stromal cells have the ability to differentiate toward a variety of connective tissue lineages including cartilage. The future use of adult mesenchymal stem cells (MSCs) for human therapies depends on the establishment of preclinical studies. Therefore, in this preclinical study we demonstrated the expression of MSC surface markers CD29, CD105, and CD44 on human bone marrow derived stromal cells during chondrogenic differentiation. Methods: Adult human bone marrow was collected from the iliac crest of 7 donors following informed consent. Mononuclear cells were isolated, incubated in monolayers, and embedded in alginate beads for three-dimensional cultures. Cellualr viability was assessed by MTT assay. Flow cytometry of alginate bead cultures was performed on days 0, 7, 14, 21, and 28 using monoclonal antibody against surface molecules, CD105, CD29, CD44, CD34 and CD45. Total contents of collagen and glycosaminoglycan (GAG) of the alginate beads was measured. SPSS 11.0 was used for data analysis. Results: After 7 days of culture, 89% of the cells expressed the human integrin beta 1 antibody, CD29. The CD29-positive cells remained elevated at 83% on days 28. However, while only 18% expressed the type II TGF-beta receptor endoglin, CD105 on day 7, the CD105-positive cells increased abruptly 65% on day 14 remaining elevated up to day 28. The expression of CD44 was maximal in the first passage cell (63%). High concentration of TGF-beta 3 (10 ng/mL) was more favorable for sustaining cell viability than a low concentration (0.5 ng/mL)(n=4, p=0.002, day 21). The total contents of collagen and GAG in the MSC-alginate beads increased during the three-dimensional culture (n=4, p=0.02, p=0.006) suggesting its differentiation into a chondrogenic lineage. Conclusion: CD29 was expressed earlier than CD105 during chondrogenic differentiation of human bone marrow MSC. CD44 expression was highest in the first passage cells and gradually decreased afterwards.

Retrovirus를 이용하여 조혈모세포에 유전자를 전달하기 위한 최적화

김상경(Sang-Gyung Kim),서헌석(Hun-Suk Suh),이종원(Jongwon Lee),신동건(Dong-Gun Shin),이재관(Jaegwan Lee),김현민(Hyun-Min Kim),김재식(jay-Sik Kim),서장수(Jang-Soo Suh) 한국생물공학회 1999 KSBB Journal Vol.14 No.5

다약제 내성 유전자를 이용한 항류마티스 약제의 내성 검사 방법 개발

김상경 ( Sang Gyung Kim ),서헌석 ( Hun Suk Suh ),최정윤 ( Jung Yoon Choe ),이종원 ( Jong Won Lee ),서장수 ( Jang Soo Suh ),김신규 ( Think You Kim ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.1

Objective: A number of disease-modifying anti-rheumatic drugs (DMARDs) have been shown to be more effective than placebo in the management of rheumatoid arthritis (RA). However, most course of DMARDs, except methotrexate, are discontinued after 2 or 3 years, because of toxicity, lack of efficacy or escape from control. The multi-drug resistance (MDR) is a phenomenon in which cells develop cross-resistance to many agents such as anthracyclin, vinca alkaloids and colchicine. In our hypothesis, MDR phenomenon could be implicated in acquired resistance to DMARDs in RA. We have established a mdr1 cell line and tested whether DMARDs are substrate for P-glycoprotein (P-gp). Methods: The mdr1-cDNA was cloned into retroviral vector, and the recombinant retroviral vector was transfected into PA317 cells. The target cells, NIH3T3, were infected with recombinant retroviruses. A colony most resistant to vinblastin was selected for the following experiments; expression of mdr1 gene in NIH3T3 cells was confirmed by RT-PCR, and biological function of mdr1 gene product, P-gp, was tested using Rhodamine-123 (Rh123) efflux assay. Resistance of the target cells expression P-gp which can survive against hydroxychloroquine (HCQ) and methotrxate (MTX) were measured by MTT assay. Results: RT-PCR for mdr1 gene showed successful transfer of the gene into the NIH3T3 cells. Rh123 assay revealed expression of P-gp on the selected cells as follows; Rh123 efflux activity of uninfected NIH3T3 cells was 6%, that of PLXSN was 0.2%, and that of selected cells was 44%. The 50% proliferation inhibitory capacity of the selected cells were twice for HCQ but there was no difference of that for MTX. Conclusion: We established a mdr1 cell line and using the cell line, HCQ was a substrate of MDR, but MTX was not related to MDR.

잦은 천명 증상의 재발과 혈장 내 VEGF 및 Plasmin 조절 인자들과의 관련성

김소연 ( So Yeon Kim ),권상미 ( Sang Mi Kwon ),박혜진 ( Hye Jin Park ),김우택 ( Woo Taek Kim ),김진경 ( Jin Kyung Kim ),최은진 ( Eun Jin Choi ),이계향 ( Kye Hyang Lee ),정혜리 ( Hai Lee Chung ),김상경 ( Sang Gyung Kim ) 대한소아알레르기 및 호흡기학회 2007 소아알레르기 및 호흡기학회지 Vol.17 No.4

식혈증후군이 동반된 성인형 스틸병 2예

김현숙 ( Hyun Sook Kim ),박근우 ( Keun Woo Park ),김수경 ( Soo Kyoung Kim ),김지영 ( Ji Young Kim ),박성훈 ( Sung Hoon Park ),신진향 ( Jin Hyang Shin ),김상경 ( Sang Gyung Kim ),최정윤 ( Jung Yoon Choe ) 대한류마티스학회 2007 대한류마티스학회지 Vol.14 No.2

Adult onset Still`s disease is an rare inflammatory disease with the characteristic of fever, skin rash, arthralgia or arthritis, lymphadenopathy, leukocytosis and multiple systemic organ involvement. Its accurate pathogenesis has not been elucidated yet. Its clinical manifestation is also very diverse, from relatively mild symptoms to severe complications such as concomitant infection, liver failure, disseminated intravascular coagulation, myocarditis, adult respiratory distress syndrome, which may lead to death in some cases. Particularly, concomitant hemophagocytic syndrome is rare complication that could induce a fatal outcome. Thus it is important to diagnose early and start treatments. Until now, it has been reported in only one case of adult onset Still`s disease in Korea. Here, we report two female cases of adult onset Still`s disease with concomitant hemophagocytic syndrome.

웹기반 임상데이터 관리 시스템 구축을 위한 프로그램 개발

신임희,김달호,김상경,손기철,박전우,곽상규,Shin, Im-Hee,Kim, Dal-Ho,Kim, Sang-Gyung,Sohn, Ki-Cheul,Park, Chun-Woo,Kwak, Sang-Gyu 한국데이터정보과학회 2012 한국데이터정보과학회지 Vol.23 No.1

컴퓨터의 발달로 인해 여러 가지 현상들이 수치화되어 데이터로 수집되고 있다. 특히 많은 양의 데이터가 각 분야별로 수집되고 있는데 이는 데이터를 기초로 한 분석과 해석을 통하여 의사 결정의 뒷받침이 되는 정보를 얻기 위해서이다. 데이터의 중요성이 부각되면서 데이터의 관리가 관심사가 되었다. 많은 연구자들이 공유할 수 있도록 서버에 데이터베이스를 구축하고 이를 웹 브라우저를 통하여 조회 및 확인하는 시스템을 구축하는 것이 필요하다. 본 연구에서는 연구자가 가장 많이 사용하는 엑셀 파일을 서버 데이터베이스에 업로드 하였고, 웹기반의 데이터베이스와 연동하여 연구자가 업로드한 데이터를 조회 및 확인할 수 있는 웹기반 프로그램을 개발하였다. 웹을 기반으로 데이터를 업로드 할 DB로 오라클을 사용하였으며 데이터베이스를 조회하기 위하여 웹프로그래밍 언어인 html, JAVA, JSP 등을 사용하여 웹기반 임상데이터 관리 시스템 구축을 위한 프로그램을 개발하였다. Various phenomenon can be expressed numerically and collected as a data due to rapid development of the computer. In particular large set of data is collected in various fields. We can obtain the information for final decision based on analysis and interpretation of the data. The issue is the management of the data as well as the importance of the data. So a system which stores the data in server and prints out the data to web browser is demanded. We uploaded the file of Excel form to server database and developed a web based program which can show the uploaded data through web based database. We used the Oracle DB for uploading and web programming language such as html, JAVA, JSP for querying the data. Finally, we developed a program for web based clinical data management system construction.

통합의료적 황달진단법개발을 위한 통계적 접근방법

신임희,곽상규,김상경,손기철,정현정,조윤정,이아진,권오승,Shin, Im Hee,Kwak, Sang Gyu,Kim, Sang Gyung,Sohn, Ki Cheul,Jung, Hyun-Jung,Cho, Yoon-Jeong,Lee, A-Jin,Kwon, O Sung 한국데이터정보과학회 2013 한국데이터정보과학회지 Vol.24 No.3

Healthcare approach in Western medicine and Korean Traditional Medicine (KTM) varies from its nature of human understanding and cultural differences. This fundamental difference in their approach of the human pathology has dualised and hindered common medical communication between the two fields of medicines. Within this current difficulty, the integrative medical service is said to become a novel method to provide the patients with the best medical care as their intent is to adapt and combine the advantages stated from the two different fields. This research paper shows the integrative approach of treating jaundice, where the symptoms of dampness and heat on Korean traditional standards are analyzed using statistical methods based on monitoring the blood test results. Therefore, we can explore an approach to diagnose and treat with comprehensive and integrative medicine algorithm. 건강 관리에 있어서 서양의학과 한의학의 접근 방법의 차이는 자연과 인간의 이해에 대한 문화적인 차이에서 비롯된다. 서양의학에서는 자연과 인간을 분리하고 인간 또한 여러 하위 시스템으로 나누고, 질병을 외부의 자극에 대한 반응이 적절하지 못하여 초래되는 것으로 보고 질병이 발생되면 시스템 별로 진단하여 치료해 왔다. 반면, 한의학의 경우 자연과 인간을 하나로 보고 인간의 건강을 자연과의 조화로운 상태로 규명하고 질병이 발생하기 전에 건강의 균형을 유지하기 위한 면역 기능을 높이는 예방적 치료를 주로 해왔다. 이러한 인간에 대한 근본적인 접근방법의 차이는 의료 전달체계를 양분화 시키고 상호 의사소통의 어려움을 야기했으나 통합 의료 서비스는 두 가지 의학의 장점을 살리고 최상의 치료 효과를 지향하는 시도라고 할 수 있다. 따라서 본 논문에서는 특정 질환인 황달에 대해 한의학적 분류 (습증, 열증)에 따른 서양의학에서 사용하는 혈액학적 검사수치를 통계적 분석기법을 사용하여 살펴보고 차이가 있는 수치를 살펴봄으로써 통합의료적 환자 진단과 치료에 적용할 수 있는 접근 방법을 살펴보고자 한다.

로버스트 베이지안 메타분석

최성미,김달호,신임희,김호각,김상경,Choi, Seong-Mi,Kim, Dal-Ho,Shin, Im-Hee,Kim, Ho-Gak,Kim, Sang-Gyung 한국데이터정보과학회 2011 한국데이터정보과학회지 Vol.22 No.3

This article addresses robust Bayesian modeling for meta analysis which derives general conclusion by combining independently performed individual studies. Specifically, we propose hierarchical Bayesian models with unknown variances for meta analysis under priors which are scale mixtures of normal, and thus have tail heavier than that of the normal. For the numerical analysis, we use the Gibbs sampler for calculating Bayesian estimators and illustrate the proposed methods using actual data. 본 논문은 독립적으로 수행된 연구결과를 합쳐서 일반적인 결론을 도출하는 메타분석을 위한 로버스트 계층적 베이지안 모형을 고려한다. 사전정보가 정규분포를 따른다는 가정 대신 정규분포의 척도혼합을 사용하여 정규분포보다 더 두꺼운 꼬리를 가지는 사전분포를 사용한다. 나아가 개별 분석의 분산이 알려져 있지 않은 경우를 계층적 베이지안 모형에 포함하여 메타분석을 수행하고자 한다. 깁스 표집을 사용하여 추정값을 계산하고, 실제 자료를 사용하여 제안된 방법을 예시한다.

류머티스 관절염과 골관절염 환자에서 Transforming growth factor β의 발현 양상

김채기,윤원찬,송용호,김상경,최정윤,Kim, Chae-Gi,Yoon, Wern Chan,Song, Yong-Ho,Kim, Sang-Gyung,Choe, Jung-Yoon 대한면역학회 2001 Immune Network Vol.1 No.3

The transforming growth $factor-{\beta}$ ($TGF-{\beta}$) is a multifunctional cytokine modulating the onset and course of autoimmune disease as shown in experimental models. In synovial inflammation, there is a potential role for $TGF-{\beta}$ in repairment, the inhibition of cartilage and bone destruction, and the down-regulation of immune response. The biologic effects of $TGF-{\beta}$ depend on the cell type, the isoform and the availability of active $TGF-{\beta}$. We investigated $TGF-{\beta}$ expression in patients with rheumatoid arthritis (RA) and compared to those of osteoarthritis (OA). And we determined a correlation between $TGF-{\beta}1$ and $TGF-{\beta}2$, and also the relationships between each $TGF-{\beta}$ isoform and the parameters for disease activity of RA. Methods: The study population consisted of 20 patients with RA and 20 patients with OA. The commercial ELISA kit was used to study $TGF-{\beta}1$ and $TGF-{\beta}2$ levels in peripheral blood (PB) and synovial fluids (SF). Results: 1) While PB $TGF-{\beta}1$ level was of no difference between RA and OA patient groups, SF $TGF-{\beta}1$ level was higher in RA group than OA group. Similarly, PB $TGF-{\beta}2$ levels of RA and OA groups was not different, but SF $TGF-{\beta}2$ levels was higher in RA group than OA group. 2) In patients with RA, the $TGF-{\beta}1$ levels were higher than $TGF-{\beta}2$ in both the PB and SF, while in patients with OA, there showed higher readings for $TGF-{\beta}1$ than $TGF-{\beta}2$ in SF but no difference between $TGF-{\beta}1$ and $TGF-{\beta}2$ levels in PB. 3) In patients with RA, there were no correlations between PB $TGF-{\beta}1$ and PB $TGF-{\beta}2$ levels, nor between SF $TGF-{\beta}1$ and SF $TGF-{\beta}2$ levels. At the same way, there was no correlation between PB $TGF-{\beta}1$ and SF $TGF-{\beta}1$ levels, nor between each levels of $TGF-{\beta}2$ in patients with RA. 4) There was also no correlation between each $TGF-{\beta}$ isoform and the parameters for disease activity such as ESR, CRP, tender joint count, swollen joint count, rheumatoid factor, and the duration of morning stiffness except between in PB $TGF-{\beta}1$ and disease duration of RA (r=0.637, p<0.01). Conclusion: Each $TGF-{\beta}$ isoforms were higher in synovial fluid of patients with RA than that of patients with OA. The data from the RA patients demonstrated different patterns of expressions of the isoforms depending on which compartment (PB or SF) was investigated. The quantification of different $TGF-{\beta}$ isoform is thought to be important when $TGF-{\beta}$ is measured under disease conditions of RA.

류마티스 관절염의 관절액 Total Fibronectin의 임상적 의의

김학준 ( Hak Jun Kim ),박정기 ( Jeong Ki Park ),여동근 ( Dong Geun Yoe ),윤원찬 ( Wern Chan Yoon ),정의달 ( Ye Dal Jung ),조선주 ( Sun Joo Cho ),김상경 ( Sang Gyung Kim ),전창호 ( Chang Ho Jeon ),김채기 ( Chae Ki Kim ),송용호 ( Yo 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.3

Objective: A study on fibronectin, which is synthesized in response to inflammatory process of joint destruction, can be of great value in identifying the mechanism of inflammation or disease activity of rheumatoid arthritis (RA). This study attempts to measure the concentrations of total fibronectin in synovial fluid of patients with RA and osteoarthritis (OA), and compare it with the clinical disease activity parameters of RA available. Methods: A total 68 patients suffering from knee pain and joint effusion was examined. Synovial fluids of thirty-eight RA patients and thirty OA patients were measured by using monoclonal fibronectin antibody. Cross-sectional analysis was undertaken to correlate the fibronectin levels of the RA patients with the clinical disease activity parameters available. Results: 1. Mean synovial fibronectin level of RA (148.4±72.6㎍/ml) was significantly higher than that of OA (39.5±16.9㎍/ml)(p<0.001). 2. The fibronectin levels in RA do not seem to have significant relationship with the parameters such as disease duration, the duration of morning stiffness, Ritchie index, ESR, CRP, and rheumatoid factor. Conclusion: In conclusion, the synovial total fibronectin concentration can clearly distinguish RA from OA. However, it would be unlikely to be used as a parameter of disease activity.