서울시 일부 냉각탑중 레지오넬라의 동정방법별 비교와 영향인자 연구

김기영 ( Key Young Kim ),김윤신 ( Yoon Shin Kim ),송재철 ( Jae Chul Song ),이수진 ( Su Jin Lee ),김성욱 ( Sung Uk Kim ),최태열 ( Tae Youl Choi ),박원석 ( Won Seok Park ),이철민 ( Chul Min Lee ) 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.3

In order to prevent and control Legionellosis outbreaks, we determined level of contamination with Legionellae and the effects on Legionella spp. of the cooling towers in Seoul City. This study was carried out to compare the Legionella spp. for 68 samples of cooling tower by conventional culture method with there samples by Multiplex PCR(polymerase chain reaction) from July to August in 2002. Also it was analyzed the distribution of Legionella subtypes, and evaluated molecular typing methods discrimination function and feasibility, using a arbitrarily primed(Ap)-PCR with M13 reverse primer. The detection positive rate of Legionella spp. was 22(32.3%) by conventional culture method and 33(48.5%) by multiplex PCR method of 68 samples. Multiplex PCR amplification showed 100% of positivity for 22 samples. Out of 22 Legionella isolates, 19 were identified as Legionella pneumophilla serogroup I and 3 as Legionella pneumophilla IV. Molecular analysis of 30 Legionella pneumophilla serogroup I showed 5 subtypes (la in isolates 17, Ib, 2; Ic, 7; II,2 ; III, 2) and Legionella pneumophilla IV showed(VI in isolates 2 V, 1) by Ap-PCR. Most of the effectives except turbidity seemed not to be significant with Legionella species isolation and it was significant to do not disinfect in multiplex PCR result. This results suggest that PCR method could be contributed to detection of Legionella spp. Also it showed that the Ap-PCR method could be used as a rapid and reproducible tool in tracking legionellosis outbreak.

HPLC와 Jaffe method의 요중 크레아티닌 및 비중이 마뇨산 보정값에 미치는 요인에 관한 연구

김기영 ( Key Young Kim ),김종규 ( Jong Gyu Kim ),윤기남 ( Ki Nam Yoon ),박화미 ( Wha Me Park ),박훈희 ( Hun Hee Park ) 한국산업보건학회 2015 한국산업보건학회지 Vol.25 No.4

Objectives: The purpose of this study was to evaluate the relevance of adjusting a urinary sample for urine hippuric correction value and its effects. Urinary biological monitoring data are typically adjusted to a constant creatinine and specific gravity concentration to correct for variable dilutions among spot samples. This study was conducted to evaluate the suitability of adjusting the urinary concentrations of urine creatinine and specific gravity(SG). Methods: We measured the concentrations of hippuric acid, in spot urine samples collected from control(119), case(120) individuals. The value of hippuric acid was adjusted by SG and urinary creatinine(HPLC & Jaffe). Results: The major results were as follows. The concentrations of urinary creatinine and SG for the control group were 1.84 g/L(SD 0.99) for arithmetic mean and 1.56 g/L(GSD 1.86) for geometric mean by HPLC method, 1.57 g/L (SD, 0.82) for arithmetic mean and 1.33 g/L(GSD 1.85) for geometric mean by Jaffe method, 1.028(SD 0.09) for arithmetic mean and 1.02(GSD 1.06) for geometric mean by refractometer. Hippuric acid levels were 0.40 g/L(SD 0.51) by arithmetic mean and 0.20 g/L(GSD 3.59). In that case the exposed group was 1.40 g/L(SD 0.58) for arithmetic mean and 1.28 g/L(GSD 1.55) for geometric mean by HPLC method, 1.27 g/L(SD 0.56) for arithmetic mean and 1.14 g/L(GSD 1.62) for geometric mean by Jaffe method, 1.045 L(SD 0.27) for arithmetic mean and 1.02(GSD 1.13) for geometric mean by refractometer(P<0.05). Hippuric acid levels were 0.67 g/L(SD 0.79) for arithmetic mean and 0.39 g/L(GSD 2.94)(p<0.05). The urine creatinine concentrations were affected by gender(p < 0.01) but SG levels were not affected by gender or age(p>0.05). After adjustment, urine hippuric acid was correlated with creatinine(HPLC & Jaffe)(r=0.723, P<0.05, r=0.708, P<0.05) and SG(r=0.936, P<0.05) and the control group shows significantly higher than the case group. In the case group for adjusted urine hippuric acid was correlated with creatinine(HPLC & Jaffe), (r=0.736, P<0.05), r=0.549, P<0.05), SG(r=0.549, P<0.05). After adjusting urine hippuric acid by urine creatinine(HPLC and Jaffe method) and specific gravity, significant associations were found between the control group and case group, respectively(r=0.832, P<0.05, r=0.845, P<0.05) and (r=0.841, P<0.05, r=0.849, P<0.05). Specific gravity adjustment appears to be more appropriate for variations in the urine creatinine method. Conclusion: we found that urinary creatinine concentrations were significantly affected by gender, and other factors and that care should therefore be exercised when correcting urinary metabolites according to the urinary creatinine concentration in spot urine. It is determined that additional study is needed for biological monitoring.

C3H6에 의한 NOx의 SCR 촉매에서 귀금속과 제올라이트가 double wash-coating된 monolith의 촉매활성

안호근 ( Ho Geun Ahn ),김동열 ( Dong Yeol Kim ),김기영 ( Ki Yeong Kim ),최영기 ( Young Key Choi ) 한국공업화학회 2007 응용화학 Vol.11 No.2

無腦兒의 一例

金基榮 全南大醫大 1974 전남의대학술지 Vol.11 No.4

자라 膀胱의 短絡電流 및 電位差에 미치는 Sympathomimetic amine 및 諸利尿薺의 影響 : Antyda japonica

金基榮 全南大醫大 1974 전남의대학술지 Vol.11 No.4

병원 조직병리 검사실내 봉입과정의 공기중 크실렌 농도와 생물학적 표식자 관련성 조사연구

김기영,김윤신 漢陽大學校 環境 및 産業醫學硏究所 1997 環境과 産業醫學 Vol.6 No.1

The objective of this study was to examine the correlation between the xylene concentration in air and the concentration of methylhippuric acid in urine of workers exposed to xylene. The atmospheric xylene concentration was analyzed by gaschromato graphy after collecting air sampled by charcoal tube, and the urinary methyl hippuric acid concentration was analyzed by high performance liquidchromato graphy after collecting 50 ml of spot urine samples collected at the end of a shift. The control group consisted of 55 workers who had never been exposed to xylene, and the exposed group of 16 workers who had been exposed to xylene at the histology(pathology) laboratories of the 16 hospitals located in Seoul. The results were as follows; 1. The mean concentrations of o-xylene, (m+p0-xylene and ethyl benzene were 4.16±4.79 ppm, 12.83±13.44 ppm and 4.18±4.46 ppm respectively. The average total concentration of xylene was 16.98±18.17 ppm. 2. Urinary concentration of 0-methylhippuric acid, (m+p)-methylhippuric and mandelic acid in the exposed group were 91.27±106.20 mg/g creatinine, 301.71±164.01 mg/g creatine and 50.29±32.76 mg/g creatinine, respectively. The total concentration of methylhippuric acid was 392.98±261.31 mg/g creatinine in the exposed group. However, methylhippuric acid was not detected at all in the control group. 3. Statistically significant relationship between xylene and methyl hippuric acid, and ethylbenzene and mandelic acid were found. 4. The compenent analysis of xylene used in the laboratories showed contain, on the average, 11.91 % o-xylene, 16.41 % p-xylene, 36.37 % m-xylene, 34.91 % ethylbenzene, and 0.36 % others. In summary although the airborne concentrations at the hisology/pathology laboratories were below current occupational exposure limits, it is recommended to perform the mouting(coverslipping) works under a well ventilated laboratory hood in order to reduce unnecessary xylene exposure. since xylene contain ethylbenzene regardless of its purity, it is recommended to monitor not only methylhippuric acid for xylene but also mandelic acid for ethylbenzene as a biological marker.

일부 전자업체 사업장의 환경위해 평가에 관한 조사연구

김기영,강혜진,이도우,김윤신 漢陽大學校 環境 및 産業醫學硏究所 1995 環境과 産業醫學 Vol.4 No.1

An investigation was carried out to obtain the information of working environmental and health conditions of workers in 3 electronic industries during October 1993-october 1994. The mean concentration of light was 481.7 Lux in three electronics industries, the mean noise level was 80.5 dB. The mean lead concentration in air was 0.002mg/㎥, the mean dust concentration was 0.136mg/㎥. The mean concentration of n-hexane in a C industry exceeded TLV. The SUSY(Subjective Symtoms) of whole workers showed the highest mean scores, respectively, followed by MENT(Mental Instability), LIFE(Irregularity of life). Particular concerns to the use of VDTs include vision, radiation, reproduction, dermatitis, stress and ergonomics.

기중 톨루엔 농도와 요중 마뇨산 및 일부 간기능 지표와의 상관성 연구 : On Workers Exposed to Mixed Organic Solvent 혼합 유기용제 폭로 근로자를 대상으로

성영모,김윤신,김기영,송재철,이종락,손정일,이수진 漢陽大學校 環境 및 産業醫學硏究所 1995 環境과 産業醫學 Vol.4 No.1

In order to certify the correlation among toluene concentration in air[A-toluene] of 41 workplaces and urinary excretion of hippuric acid[U-hippuric acid] of their workers, and their liver fuction, from the 676 workers exposed to mixed organic solvent including toluene, the authors investigated A-toluene, U-hippuric acid and some liver function index. The results were as follows : 1. The study population were devided to 3 group[group Ⅰ ; 0.11, group Ⅱ ; 1.80, group Ⅲ ; 18.09 ppm(mean)] according to A-toluene, and mean U-hippuric acid of group Ⅰ, Ⅱ and Ⅲ were 0.51±0.44, 0.56±1.31, 0.72±0.61, respectively. And significant differences were being between group Ⅰ and Ⅲ, Ⅱand Ⅲ(p<0.05). 2. The authors excuted the multiple logistic regression analysis to search significant factors which influence to U-hippuric acid. The significant factors were A-toluene(p=0004) and sex(p=0.0315). 3. Correlation coefficient between U-hippuric acid and A-toluene was 0.1181(p=0.002) and that between U-hippuric acid and liver fuction index were -0.0990(p=0.010) for ALT, -0.1304(p=0.001) for AST and -0.1480(p=0.012) for -GTP. 4. In order to obtain the prediction model between A-toluene and U-hippuric acid, the authors excuted the simple linear regression analysis, and the regression function were Y=0.5696+0.0045X(p=0.0022).

Bio-Filter의 미생물성물질에 대한 성능 및 효과에 대한 연구

문길주,김기영,강혜진,김윤신,허진 漢陽大學校 環境 및 産業醫學硏究所 1995 環境과 産業醫學 Vol.4 No.1

This study measured and analyzed the concentration of the microbes contained in the exterior air and interior air of duct of Bio-Filter system installed at KIST utilizing RCS Air Sampler and Blood Agar Petridish(BAP) from 13 September 1993 to 22 December 1993 in order to analyze the sterilizing capability and efficiency against the microbes of Bio-Filter. As a result of this experimentation, in exterior air and interior air of Bio-Filter system 3 kinds og germ group such as G(+) cocci, G(_) bacilli and G(+) bacilli were cultured and these germs were identified as that they have about ten kinds of characteristics. By comparision of the number of colony according to the kind of germ, in the exterior air G(+) cocci was cultured to each about 6.75 colonies in average and it was an extreme many, while in the system G(+) bacilli was cultured about 10 colonies before filter and it showed extreme many. When we check the decreasing rate of the kind of germ before and after operation of Bio-Filter, we can see that G(+) cocci has been decreased by 87.5%, G(_) bacilli by 85.7% and G(+) bacilli by 87.5% respectively.

우리나라 전자업체 사업장의 유해물질 평가 및 근로자의 건강영향에 관한 기초조사연구

박종안,김윤신,고응린,김기영,장지훈 漢陽大學校 環境 및 産業醫學硏究所 1992 環境과 産業醫學 Vol.2 No.1

In Korea, electronics industries as major export industries have been markelly increased and could be one of major issues in the field of occupational health risks. An investigation was carried out to obtain the basic information of working environmental and health statics of workers in 4 electronics industries in Korea during June Oct. 1992. The mean concentration of light was 367.6 Lux in four electronics industries, the mean noise level was 79.6 dB. The mean lead concentration in air was 0.0098 mg/m3 in a big industry, while the mean concentration in air was 0.021 mg/m3 in a small industry. In general, the mean concentration of each pollutant did not exceed the TLV. Major occupational risk factors found in electronics industries female workers and blue collar worker.