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B형 간염 바이러스의 만성 감염 및 수직 감염 예방과 HLA 대립유전자와의 연관성
김종현(Jong-Hyun Kim),표철우(Chul-Woo Pyo),허성숙(Seong-Suk Hur),김양겸(Yang-Kyum Kim),고대균(Dae-Kyun Koh),오진희(Jin-Hee Oh),허재균(Jae-Kyun Hur),강진한(Jin-Han Kang),백순영(Soon-Young Paik),유문간(Mun-Gan Rhyu),김금용(Gum-Ryong Ki 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.3
Herpes simplex virus-thymidine kinase 유전자가 전이된 종양 세포에서 Gancyclovir와 방사선 조사에 의한 항 종양 효과
이재우,오승택,안창혁,임근우,조현일,김금용,김태규,Lee, Jae Woo,Oh, Seong Taek,Ahn, Chan Hyuk,Lim, Kun Woo,Cho, Hyun-Il,Kim, Gum Ryong,Kim, Tai-Gyu 대한면역학회 2001 Immune Network Vol.1 No.1
Background: Many types of cancer become resistant to current chemotherapeutic and radiotherapeutic intervention. To overcome this situation application of gene therapy by the introduction of suicide genes followed by their prodrugs may be promising. A viral enzyme, Herpes simplex thymidine kinase (HSV-tk), which converts ganciclovir from an inactive prodrug to a cytotoxic agent by phosphorylation, are being actively investigated for use in gene therapy for cancer. The purpose of this study was to determine whether combining prodrug-activating gene therapy and irradiation might result in enhanced antitumor effects. Methods: The HSV-tk gene was cloned into the retroviral vector, pLXSN and established the clones producing retroviruses carrying the HSV-tk gene. The carcinoma cell line, HCT116 and Huh-7 were transduced with high-titer recombinant retroviruses. These cell lines were treated with ganciclovir before or after irradiation for the defining combinational effect of suicide gene therapy and radiotherapy. Results: The titers of cloned PA3 17 amphotropic retroviruses ranged from 4 to 6 X $10^6CFU/ml4$. After selectional periods, the expression of HSV-tk was confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). The growth of cells expressing HSV-tk was inhibited as increase of GCV dose after 48 hr and the growth inhibitory effect of GCV was much higher after 72 hr. When the cells transduced with HSV-tk gene were exposed to radiation, the growth inhibitory effect of GCV was significantly increased, as compared with non-transduced parental cells. Conclusions: The results suggest that the addition of HSV-tk gene therapy to standard radiation therapy may improve the effectiveness of treatment for solid tumors.
한국형 출혈열 및 만성간염과 조직적합성 항원간의 유전적 관련성에 관한 연구 (Ⅱ) : (Ⅱ)만성간염과 조직적합성 항원간의 유전적 관련성에 관한 연구
한훈(Hoon Han),김태규(Tae-Kyu Kim),유문간(Moon-Gun Rhyu),임병욱(Byung-Uk Lim),김금용(Gum-Ryong Kim),이종훈(Chong-Hoon Lee),김부성(Boo-Sung Kim),김호연(Ho-Youn Kim),윤영석(Young-Suk Yoon),방병기(Byung-Kee Bang),민병석(Byong-Sok Min) 大韓微生物學會 1986 大韓微生物學會誌 Vol.21 No.2