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인체세포주에서 저선량 99mTc 에 의해 발현되는 방사선 적응반응에 관련된 유전자에 관한 연구
권안성(An Sung Kwon),범희승(Hee Seung Bom),최찬(Chan Choi),김지열(Ji Yeul Kim),임욱빈(Wook Bin Lim) 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.5
N/A Purpose: The purpose of this study was to search activated genes that could be related to radiation adaptive response (RAR) induced by low-level radiation from 99mTc in human cell lines. Methods : We used gene discovery array (GDA) and representational difference analysis (RDA) methods. 99mTc-pertechnetate was added to 2 x 106/mL NC-37 cells (human lymphoblastic cells) to make concentrations ranging from 148 MBq/mL to 148 Bq/mL by serial 10 fold dilutions. After 44 hours, 2 Gy gamma irradiation was given to them using a Cs-137 cell irradiator. Results: As compared to the control (Con) group to which no 99mTc was added, those cells to which 148 and 14.8 KBq of 99mTc were added showed significantly lower damage to chromosomes, which was evaluated by metaphase analysis. Cells with 148 KBq 99mTc (T148 group) showed most significant protection. Activated genes in the T148 group as compared to Con group were evaluated by GDA and RDA methods. GDA revealed genes of casein kinase 2 (CK2) beta chain, immunoglobulins (Ig), human leukocyte antigen (HLA)-B, and two novel genes. Twenty RAR related clones were selected by RDA method. The size of those genes was from 234 to 603 base pairs. Conclusions: RAR was induced by low dose irradiation from 99mTc in NC-37 cell lines. Genes related to the response included CK2, Ig, HLA-B in human lymphoblastic cell lines. (Korean J Nucl Med 2001:35: 313-323)
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Tc-99m MDP와 Tc-99m DTPA 신티그라피를 시행한 환자의 말초혈액 림프구에서 유도되는 방사선 적응반응의 비교
범희승(Hee Seung Rom),이명호(Ming Hao Li),민정준(Jung Jun Min),권안성(An Sung Kwon),김지열(Ji Yeul Kim) 대한핵의학회 2000 핵의학 분자영상 Vol.34 No.3
N/A Purpose: The purpose of this study was to compare the radiation adaptive response (RAR) in peripheral lymphocytes (PL) of patients induced by Tc.-99m MDP and Tc-99m DTPA scintigraphies. Materials and Methods: Lymphocytes from 45 patients (25 males, 20 females, mean age 44±18 years) were collected before and after scintigraphies using 740 MBq Tc-99m MDP (n=22) or Tc-99m L)TPA (n=23). Lympho-cytes from 20 controls (12 males, 8 females, mean age 43±7 years) were also callected. They were exposed to challenge dose of 2 Gy γ-rays using a Cs-137 cell irradiator, Number of ring-form (R) and dicentric (D) chromosomes was counted under the light microscope. From them a representative score, Ydr, was calculated as Ydr=(D+R)/cells. Adaptation index (AI) was defined as difference of Ydr between unconditioned and conditioned lymphocytes. Ydr was also measured after an administration of cyclohexi-mide (CHM), a protein synthesis inhibitor, before challenge dose. Results: RAR was induced in both groups of patients. CHM abolished the adaptive response in both groups. AI of Tc-99m MDP group was significantly higher than that of Tc-99m DTPA group. Conclusion: Tc-99m MDP induced RAR was more prominent than those induced by Tc-99m DTPA. (Korean J Nucl Med 2000;34:252-9)
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안구적출에 따른 위둔덕의 칼슘결합단백질의 재구축 및 상호 연관성
안병수,고길석,안명수,김경주,권안성,정명섭,박춘매,조병옥,김진우,Samudra Acharya,Parmeshwar Narayan Amatya,장인엽 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1
Background: Superior colliculus is a part of midbrain, and participates in the visual reflexes, It receives afferent fibers from optic nerve, visual cortex, and spinotectal tract. After optic deprivation, the microscopic structure of the superior colliculus changed. Calcium-binding proteins (CBPs) Play an important role in the neuronal protection, differentiation and reorganization of the central nervous system, Objectives and Methods: The effects of neonatal retinal deafferentation on a CBPs, calbindm D-28k (CB), Parvalbumin (PB) and calretimn (CR), and the existence of colocalization between the CBPs were examined immunohistochemically in the rat superior colliculus. Results: On the experimental (contralateral to enucleation) side of superior colliculus, the number of CB-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. The number of PB-IR neurons and fibers was also reduced on the experimental side (88.5% compared to control), In the other hand, the CR-IR cells were dramatically increased (642% compared to control), but CR-IR fibers were markedly decreased on the experimental side. The colocalization between CB-CR and PV-CR was rarely observed in the superior colliculus Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively stable in CB- and PV-IR neurons and plastic in CR-IR neurons.
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權熙勳,安聖洙 忠州大學校 1983 한국교통대학교 논문집 Vol.16 No.2
Korean industrial situations are very rapidly changing. Sophisticate pattern of industry is resulting in the numbers and kind of workers who are need in industry. The more sophisticated the industry, the more crucial the technician's role. In order to educated electronic techician necessary to the electronic industrial society during the short period of two year course of jonior technical college, this paper suggest the educational method and curriculum which are proper to the requirement of radically developing electronic industry. In undertaking the construction of this curriculum for electronic technology based upon job analysis and studying a curriculums, this curriculum promote a good all-round education & trainning in electronic technology which will be enable to find empolyment in a wide variety of positions in electronic industry. This paper look into the problems of the junior technical college and suggest directions method for improvement of the junior technical college education.
ScienceON
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