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Helicobacter pylori 감염 위 점막 조직에서 단백체 발현 양상에 대한 연구
강호석 ( Ho Suk Kang ),홍성노 ( Sung Noh Hong ),박혜림 ( Hye Rim Park ),권미정 ( Mi Jung Kwon ),이준행 ( Jun Haeng Lee ),김재준 ( Jae J Kim ) 대한소화기학회 2014 대한소화기학회지 Vol.64 No.1
목적: H. pylori는 위점막 손상과 위암 발암 과정을 시작하는 요인으로 생각된다. 이번 연구는 H. pylori 감염 위점막의 단백체 분석(proteomics)을 통해 H. pylori 감염과 연관되어 발현에 차이를 보이는 단백질을 확인하고자 하였다. 대상 및 방법: 상부위장관검사를 이용하여 H. pylori 양성 위점막 조직 15예와 H. pylori 음성 위점막 조직 15예를 얻었다. 이에 대해 단백질 분리, 2차원 전기영동, 그리고 단백질 질량분석을 시행하였다. 결과: H. pylori 양성 위점막에서 Keratin 1, ezrin, ATP synthase subunit alpha mitochondrial isoformc, Keratin type I cytoskeletal 19 등의 단백질이 과발현됨을 확인하였고, 반면 71 kd heat shock cognate protein, ATP synthase subunit alpha mitochondrial precursor, annexin IV 등이 저발현됨을 확인하였다. 이들 단백질 중 Western blot과 면역화학염색을 통해 ezrin의 발현이 H. pylori 양성 위점막에서 증가함을 다시 한번 확인하였다. 결론: 이번 연구는 ezrin 단백질이 향후 H. pylori 연관 위염의 분자표지인자 등으로 사용될 수 있음을 제시하였다. Background/Aims: Helicobacter pylori infection is linked to the development of gastric cancer. H. pylori associated gastric inflammation is considered to be the first important step in the histogenesis of such neoplasia. However, studies that compare proteome of gastric mucosa infected with or without H. pylori are lacking. Methods: We employed proteomics analysis on the endoscopic biopsy specimens of gastric mucosa obtained from two groups (30 cases): healthy subjects without H. pylori infection (15 cases), and gastritis patients with H. pylori infection (15 cases). The pooled proteins obtained from gastric mucosa infected with or without H. pylori were separated by two dimensional gel electrophoresis and analyzed by a computer-aided program. The altered protein expressions were then identified by mass spectrometry and validated by Western blotting and immunohistochemistry. Results: On mass spectrometry using MALDI TOF™ Analyzer, the up-regulation of Keratin 1, ezrin, adenosine triphosphate (ATP) synthase subunit alpha mitochondrial isoform c, Keratin type I cytoskeletal 19, and Keratin type I cytoskeletal 9 were identified; in contrast, 71 kd heat shock cognate protein, ATP synthase subunit alpha mitochondrial precursor, and annexin IV were down-regulated. Among them, membrane cytoskeleton linker ezrin was validated using Western blot and immunohistochemistry. Conclusions: Expression of ezrin was significantly different between the gastric mucosa with and without H. pylori infection. Therefore, ezrin could be considered a promising potential molecular marker for detecting H. pylori infection in gastric mucosa. (Korean J Gastroenterol 2014;64:10-17)
성인에서 상피하 종양으로 나타난 대장의 미만성 신경절신경종증 2예 보고
김태준 ( Tae Jun Kim ),임현 ( Hyun Lim ),강호석 ( Ho Suk Kang ),문성훈 ( Sung Hoon Moon ),김종혁 ( Jong Hyeok Kim ),박충기 ( Choong Kee Park ),권미정 ( Mi Jung Kwon ),이봉화 ( Bong Hwa Lee ) 대한소화기학회 2015 대한소화기학회지 Vol.66 No.2
Colonic diffuse ganglioneuromatosis is a benign neoplastic condition characterized by disseminated, intramural, or transmural proliferation of neural elements involving the enteric plexuses, sometimes associated with von Recklinghausen’s disease and other multiple tumor syndromes. Colonic diffuse ganglioneuromatosis is usually large, ranging from 1 to 17 cm, and thus can distort the surrounding tissue architecture as well as infiltrate the adjacent bowel wall. However, colonic diffuse ganglioneuromatosis is an exceptional finding in adults and only individual cases are reported in the literature. Herein, we report two unusual cases of adult patients with colonic diffuse transmural ganglioneuromatosis presenting as a large subepithelial tumor. (Korean J Gastroenterol 2015;66:111-115)
생체외 ( In - Vitro ) 에서 직접 용해제에 의한 간내 담석 용해도의 고찰
박찬욱(Chan Wook Park),류권호(Kwon Ho Ryu),권강호(Kang Ho Kwon),이종태(Jong Tae Lee),이준성(Joon Seong Lee),김연수(Yun Soo Kim),이문성(Moon Sung Lee),황성규(Seong Gyu Hwang),김선주(Sun Joo Kim),심찬섭(Chan Sup Shim),(H . Igimi) 대한내과학회 1996 대한내과학회지 Vol.50 No.4
N/A Objectives: The incidence of intrahepatic duct (IHD) stone is higher in east Asia including Korea than western contries. IHD stone has been regarded as a troublesome disease with poor surgical results. Recently, percutaneous transhepatic choledochoscopic lithotripsy with electrohydraulic lithotripsy of IHD stones showed good success rate, but it also couldn't remove stones in case of severe stenosis, acute angulation or peripheral impacted stones. A MTBE, widely used for dissolving cholesterol stone, was considered to be little effect for dissolving pigment stones. So we analysed IHD stones and dissolved them by direct solubilizer to investigate the dissolution rate of IHD stones. Methods. We have analyesd the compositions of 13 IHD stones removed during the percutaneous transhepatic cholangioscopy or pos-operative cholangioscopy by infrared spectrophotometer and dissoluted by direct solubilizer. Dry intact IHD stones were weighed and then inserted into 50ml glass tube. Within an 8hours incubation time, the 25ml of solvent were exchanged every 30minutes. After 2 and 8hours of incubation, the stone residue was dried-up at 60℃ for check weight reduction and compare to the initial dry weight. The solvent A medium was a 0.1M boric acid/sodium carbonate buffer (pH 9.4), EI)TA 1%(w/v), 1 M urea and solvent B was solvent A medium plus 0.04 M Lubrol PX and N-acethylcysteine. Results: The sites of dissolved IHD stons were both IHD stones 8cases(61.5%), left IHD stones 3(23.1%) and right IHD stones 2(15.4%), The components of IHD stones were calcium bilirubinate stones in 11cases(84.6%), followed by mixed stones 2 (15.4%). The dissolution rates after 2 and 8hours dissolution by solvent A is 11.7%, 24.7% and by solvent B is 25.7%, 62.7% respectively. The dissolution rates after 8hours in solvent B is higher than solvent A (p<0.05). Conclusion: In order to improve dissolution rates of IHD stones, the development of new solvent and the improvement of currently used solvents and methodology should be considered.
경피경간 담낭배액술 및 경피적 복막 배액술로 치유된 담낭천공 1예
박찬욱,이준성,이문성,조성원,심찬섭,송동화,권강호,홍수진 대한소화기내시경학회 1994 Clinical Endoscopy Vol.14 No.4
Perforation of gallbladder is a serious complication of acute cholecystitis with alarmingly high mortality rate. These high mortality and morbidity rates were caused by delay in prompt diagnosis and adequate therapy. Especially, mortality and morbidity rates rise markedly in the elderly patient with severe systemic illness. In the patients of gallbladder perforation who are poor candidate for general anesthesia and major operation, percutaneous cholecystic drainage procedure is good alternatives. We experienced a case of gallbladder perforation which was treated successfully by non-operative percutaneous transhepatic cholecystic drainage(PTCCD) in 65-year-old female. She couldn't be a candidate for cholecystectomy or operative chlecystostomy because of severe adhesion of gallbladder to adjacent organ and tissue due to previous gallbladder empyema. We decided to take non-operative percutaneous transhepatic cholecystic drainage and percutaneous peritoneal drainage of abdominal abscess. Thereafter, we examined gallbladder by percutaneous transhepatic cholecystoscopylPTCCS) and rule out gallstone and gallbladder malignancy. So, we presented the case with the brief review of the literatures.
박찬욱,문철,이동화,이준성,이문성,조성원,심찬섭,진소영,송동화,권강호,홍수진 대한소화기내시경학회 1994 Clinical Endoscopy Vol.14 No.4
Gastational choriocarcinoma is a malignant neoplasm of trophoblast. It may occur after hydatidiform mole, spontaneous abortion, normal pregnancy, and even an ectopic pregnancy. Extragenital choriocarcinoma is a rare tumor which attracts interest because of its controversial pathogenesis. It has been reported to occur within the lung, mediastinum, breast, prostate, thymus, pineal, nose, liver, bladder, and biliary tree, as well as most parts of the gastrointestinal tract. We experienced a case of primary choriocarcinoma of stomach with a metastasis to the liver of a 54-year-old man. So, we present a case with a review of literature.