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누에 핵다각체병 바이러스의 Ecdysteroid UDP-glucosyltransferase 유전자가 누에의 발육에 미치는 영향
강경돈,이은정,Shizuo George Kamita,성수일 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
The baculovirus egt gene encodes an ecdysteroid UDP-glucosyltransfernse(EGT) which catalyzes the transfer of glurose from UDP-glucose to the insect molting hormone ecdysteroid resulting in a functionally inactive ecdysteroid. In baculovirus-infected insect larvae, EGT has been shown block molting and pupation. In this study, we compared the development of 4th and 5th instar silkworm, Bombyx mori, larvae injected with either wild-type Bombyx mori nucleopolyhedrovirus (BmNPV) or a mutant BmNPV(BmEGTZ) in which the egt gene was disrupted by the insertion of a lacZ gene cassette. Larvae injected with BmEGTZ died roughly 12 h more rapidly compared to identical larvae infected with BmNPV. In addition, BmEGTZ-infected larvae prematurely stopped feeding and gain less weight compared to BmNPV-infected larvae. In order to investigate why BmEGTZ-infected larvae died more rapidly than BmNPV-infected larvae, the array of hemolymph proteins in BmEGTZ- or BmNPV-infected larvae were analyzed by SDS-PAGE. The hemolymph of BmEGTZ-infected larvae showed virus-specific proteins, including polyhedrin, about 12 h earlier than the hemolymph of BmNPV-infected larvae
Identification of the Genes Involved in 1-Deoxynojirimycin Synthesis in Bacillus subtilis MORI 3K-85
강경돈,조용석,송지혜,박영식,이재연,황교열,이상기,정지형,권오석,성수일 한국미생물학회 2011 The journal of microbiology Vol.49 No.3
1-Deoxynojirimycin (DNJ), a D-glucose analogue with a nitrogen atom substituting for the ring oxygen,is a strong inhibitor of intestinal α-glucosidase. DNJ has several promising biological activities, including its antidiabetic, antitumor, and antiviral activities. Nevertheless, only limited amounts of DNJ are available because it can only be extracted from some higher plants, including the mulberry tree, or purified from the culture broth of several types of soil bacteria, such as Streptomyces sp. and Bacillus sp. In our previous study, a DNJ-producing bacterium, Bacillus subtilis MORI, was isolated from the traditional Korean fermented food Chungkookjang. In the present study, we report the identification of the DNJ biosynthetic genes in B. subtilis MORI 3K-85 strain, a DNJ-overproducing derivate of the B. subtilis MORI strain generated by γ-irradiation. The genomic DNA library of B. subtilis MORI 3K-85 was constructed in Escherichia coli, and clones showing α-glucosidase inhibition activity were selected. After DNA sequencing and a series of subcloning, we were able to identify a putative operon which consists of gabT1, yktc1, and gutB1 genes predicted to encode putative transaminase, phosphatase, and oxidoreductase, respectively. When a recombinant plasmid containing this operon sequence was transformed into an E. coli strain, the resulting transformant was able to produce DNJ into the culture medium. Our results indicate that the gabT1, yktc1, and gutB1genes are involved in the DNJ biosynthetic pathway in B. subtilis MORI, suggesting the possibility of employing these genes to establish a large-scale microbial DNJ overproduction system through genetic engineering and process optimization.
강경돈,이기훈,정병희,류운영,남중희 한국잠사학회 1999 한국잠사곤충학회지 Vol.41 No.3
The silk/paper mulberry paper was prepared by recycling of the hard twisted silk waste and its mechanical properties was investigated. The silk waste was pretreated with sodium hydroxide (0.5%)and sodium carbonate (4%) to make it easy for heating and fibrillation. The silk/paper mulberryh and sheet paper has better mechanical properties of tearing strength and air permeability compared with the traditional and modified traditional paper. Treating with alkaline solution, the hard twisted silk waste could be used for an novel composite material.
Comparative Analysis of α-glucosidase Activity in Bombyx mori and Antheraea yamamai
강경돈,Shizuo George Kamita,Koichi Suzuki,성수일 한국잠사학회 2010 International Journal of Industrial Entomology Vol.21 No.2
α-Glucosidase (EC 3.2.1.20) is a glycosidase that hydrolyzes disaccharides, oligosaccharides, and polysaccharides resulting in the release of α-D-glucose. In this study, α-glucosidase activity in the hemolymph and midgut of the mulberry silkworm Bombyx mori and Japanese oak silkmoth Antheraea yamamai was measured using maltose,sucrose, trehalose, and p-nitrophenyl α-D-glucopyranoside as substrates. In general, hemolymph α-glucosidase activity was higher in B. mori than in A. yamamai. In contrast, midgut α-glucosidase activity was higher in A. yamamai than in B. mori for all of the substrates tested. α-Glucosidase activity in the midgut of both B. mori and A. yamamai showed similar responses to changes in pH and temperature for all of the substrates tested. Native (7.5%) PAGE of hemolymph and midgut proteins from B. mori and A. yamamai followed by staining with 4-methylumbelliferyl α-D-glucoside (MUG) indicated that the α-glucosidases of these related lepidopterans are functionally similar but structurally different. In comparison to α-glucosidase activity from A. yamamai, α-glucosidase activity from B. mori was generally less sensitive to the α-glucosidase inhibitors, 1-deoxynojirimycin (DNJ), acarbose, and voglibose when the activity was determined using maltose,sucrose, and trehalose.
Effect of Low Molecular Weight Silk Fibroin on the Inhibition of Tyrosinase Activity
강경돈,이기훈,박영환,신봉섭,남중희 한국잠사학회 2004 International Journal of Industrial Entomology Vol.9 No.1
Low molecular weight silk fibroin (LMSF), which was prepared by hydrolysis of silk fibroin using hightemperature and high-pressure method, was found to inhibit the oxidation of L-3,4,-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase (EC 1.14.18.1). LMSF contained mostly free amino acids such as L-glycine, L-alanine, and L-serine and oligopeptides, mainly glycine-alanine dimer. As a result of analyzing the inhibition kinetics from Lineweaver- Burk plots, L-glycine and glycine-alanine dimer showed noncompetitive behavior while uncompetitive behavior was observed in L-alanine, and Lserine. When weight percent concentration of ID50 was compared, L-glycine was most effective on the inhibition and LMSF was also good enough for the inhibition effect of tyrosinase activity. LMSF showed a mixed-type inhibition and the inhibitory mechanism of LMSF might be caused by free amino acids and oligopeptides. As a result of spectroscopic observation with time, initial rate of increase of DOPAchrome decreased remarkably and the time to reach maximum absorbance increased as an increase of the concentration of L-glycine, meaning that L-glycine made itself mainly responsible for the formation of chelate with Cu2+ in tyrosinase. However, in case of Lalanine, L-serine, and especially glycine-alanine dimmer, the production of DOPAchrome after an arrival at maximum absorbance decreased, indicating the production of adducts through the reaction with DOPAquinone.
파이프라인 하이퍼큐브에서의 효율적인 정수 정렬 알고리즘
강경돈(Kyoung Don Kang),유관우(Kwan Woo Ryu) 한국정보과학회 1991 정보과학회논문지 Vol.18 No.6
본 논문에서는 파이프라인 하이퍼큐브(pipelined hypercube)에서의 몇 가지 기본적인 라우팅 문제에 관해 연구하고, 그 연구 결과들을 이용하여 0과 p^(0(1))(p의 상수 승) 사이의 n=p^(1+1/k)(k>0)개의 정수를 p-프로세서 파이프라인 하이퍼큐브에서 효율적으로 정렬(sorting)할 수 있음을 보인다. 본 논문에서 고려될 정렬 알고리즘은 columsort[9]와 cubesort[4]이다. Cubesort는 p-프로세서 파이프라인 하이퍼큐브에서 O(k³(n/p)) 시간에 수행될 수 있음을 보인다. 이 알고리즘은 k가 상수일 때 O(n/p)의 시간 복잡도를 가지므로 최적 알고리즘이 된다. Columnsort도 k가 상수일 때, p-프로세서 파이프라인 하이퍼큐브에서 O(n/p) 시간에 수행되어 최적 알고리즘이 된다. In this paper, we present some basic routing algorithms on the pipelined hypercube, and by using these algorithms we show that n=p(1+1/k)(k>0) elements can be efficiently sorted on the p-processor pipelined hypercube when each of these elements is an integer between 0 and p^(0(1)). We consider columnsort[9] and cubesort[4] algorithms. Cubesort can be performed on the p-processor pipelined hypercube in O(k³(n/p)) time, and it is an optimal algorithm if k is a constant. Columnsort can also be performed in O(n/p) time, which is optimal, on the p-processor pipelined hypercube if k is a constant.