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      • 土壤의 몇가지 物理的, 化學的 性質이 煙草植物의 이온平衡(Ionic Balance)에 미치는 影響

        金才正 충북대학교 엽연초연구소 1978 煙草硏究 Vol.5 No.-

        An experiment with the tobacco plant was conducted in the pots. A sandy humic soil was used with 2 levels of pH,3.5, and 5.8, with 2 kinds of anions, Cl as NH4Cl and SO4 as (NH4) 2SO4, and with 4 levels of pF, 1.5, 2.0, 2.5, and 3.5. The pH-treatment created different N-forms; NH4 at low pH(3.5) and NO3 at high pH (5.8) At low pH with high concentration of NH4 given as NH4Cl, the high content of Cl in tobacco resulted in plants suffering from NH4- and Cl-toxicity, as well as Mn-toxicity. As a result of this a small and extremly abnormal growth was clearly appeared. In the tobacco grown at low pH with NH4 given as (NH4)2SO4, a large amount of the NH4-uptake developed Mg-and Ca-deficiency. The plants grown at high pH were all healthy, however, dry matter production was higher and maturity faster when SO4 was used as anion compared to Cl. It seems that at both pH values, the N-metabolism in the plants growing on the SO4 treatment is more active than in those growing on the Cl treatment. High moisture content in soil is necessary for a good development and growth of the tobacco plant. Phosphate uptake seemed to be limitted at higher moisture stress. The dry matter yields of tops and roots, respectively, were in the order of pF 1.5>2,0>2.5>3.5. Date of chemical analysis and dry matter yields of tops and roots showed that the tobacco plant followed the normal (C-A) concept. In the normal growth of plants, the carboxylate contente of tops was quite comparable to the estimated (C-A) values. If NH4 content of plants remains in quite high quantities, it must be analysed and take into consideration for the (C-A) calculation. Al is net transported towards tops in toxic amounts due to its high immobility, it mostly stay in or on the roots, probably precipitated as a salt, When Al is present in high quantities, it has to be considered into the (C-A) calculation.

      • 地下灌水에 의하여 栽培된 煙草葉의 化學成分에 關한 硏究 : 地下水位가 다른 土壤에 대하여 In Soil with Different Ground Water Level

        金才正,趙成鎭 충북대학교 엽연초연구소 1983 煙草硏究 Vol.10 No.-

        Leaves of the tobacco plant, which was grown on the soil with different ground water level, were chemically analysed with respect to inorganic cations and anions. The followings are results. 1.It was regarded that (C-A) content of tobacco leaf was around 2,000 meq/kg D.M., as a constant value, regardless of ground water level in soil where tobacco was grown. 2.In tobacco grown on soil with higher ground water level, (C-A) content of every position of leaves was much higher than organic N content, but in upper leaf of tobacco grown with lower ground water level vice versa. The difference between (C-A) content and organic N content was much bigger in upper leaves of tobacco grown on soil with deeper ground water level. At the pot with 150cm ground water level the relation was organic N content>(C-A) content in upper leaves, organic N=(C-A) in middle leaves and organic N<(C-A) in lower leaves.

      • 四肢動脈縫合手術의 實證的 硏究

        金在正 우석대학교 의과대학 1967 우석의대잡지 Vol.4 No.1

        In recent years, a great progress has been made in the field of the small arterial surgery. The outcome of the arterial sutures, however, is not always favorable nor the technic is well popularized. It is an essential provision to master the technic of suture anastomsis of small arteries to be able to provide adequate measured for the vascular injuries encountered in the traumatic conditions of the limbs which otherwise may make it inevitable to sacrifice the parts. In order to get experienced with the suture anastomosis technic in the small arteries and to find a way to prevent the thrombus formation which is said to be a major obstacle in such a surgery, author performed following experimental surgery e.g., 12 femoral arteries in 6 mongrel dogs were divided and resutured employing various suture methods and local irrigation. Following is the summary of the findings: 1. Patency rate of the suture anastomosis in small arteries varying from 2.5 to 3.5 ㎜, in diameter was 83.3 per cent which is a rather excellent ratio in view of the reported series. 2. A beneficial effect of the heparin local irrigation in preventing the thrombus formation was not substantiated. 3. Regarding the method of small arterial anastomosis, interrupted simple everted sutures appear to be superior to running everted sutures.

      • KCI등재

        토양(土壤)의 몇가지 이화학적조건(理化學的條件)이 연초(煙草)의 생육(生育) 및 이온평형(平衡)에 미치는 영향(影響) I. 토양(土壤)의 pH, pF와 음(陰)이온(Cl<sup>-</sup>, SO<sub>4</sub><sup>-</sup>)이 연초(煙草)의 생육(生育) 및 이온평형(平衡)에 미치는 영향(影響)

        김재정,조성진,Kim, Jai-Jong,Cho, Seong-Jin 한국토양비료학회 1981 한국토양비료학회지 Vol.14 No.3

        토양(土壤)의 몇가지 물리적(物理的), 화학적성질(化學的成質)이 연초식물(煙草植物)의 생육(生育) 및 Ionic Balance에 어떤 영향을 미치고 있는가를 연구(硏究)하기 위하여 폿트실험을 하였다. 공시(供試)된 토양(土壤)은 pH를 3.5와 5.8인 두가지로 조정하였고 이에 2종류의 음(陰)이온을 ($NH_4Cl$의 Cl, $(NH_4)_2SO_4$의 $SO_4$) 처리하였고, 4수준(水準)의 pF치(値)(1.5-2.0-2.5-3.5)를 두었다. 그 결과(結果)를 요약(要約)하면 다음과 같디. 1. 산도(酸度)가 높은 토양(土壤)(pH3.5)에 $NH_4Cl$이 가(加)해진 경우 연초(煙草) Cl의 함량(含量)이 높았으며, $NH_4$와 Cl의 해작용(害作用)뿐아니라, Mn의 독작물(毒作用)까지 받았으므로 거의 생장(生長)하지 못하고 대단히 비정상적(非正常的)인 생육(生育)을 하였다. 한편 ($(NH_4)_2SO_4$를 많이 흡수하므로 Mg와 Ca의 결핍증(缺乏症)을 보였다. 그러나 산도(酸度)가 낮은 토양(pH5.8)에 가(加)해진 $NH_4-N$은 질산화작용(窒酸化作用)에 의하여 모두 $NO_3-N$로 변하였으며, $NH_4-N$와 함께 가(加)해진 Cl이나 $SO_4$에 관계없이 연초(煙草)는 건전한 생육(生育)을 하였으며 Cl 보다는 $SO_4$가 가(加)해진 토양(土壤)에서 자란 식물(植物)이 건물중(乾物重)도 더 높고 성숙도(成熟度)도 더 빨랐다. 2. 토양(土壤)의 수분함량(水分含量)은 식물(植物)이 인산(燐酸)을 흡수하는데 제한인자(制限因子)가 되는 것으로 생각되며, 지상부(地上部)와 뿌리의 건물수량(乾物收量)은 pF 1.5>2.0>2.5>3.5>의 순서이었고 pF3.5은 연초(煙草)의 일시계절점으로 생각되었다. 3. 연초식물(煙草植物)의 화학분석치(化學分析値)와 건물수량(乾物收量)은 이 식물(植物)이 정상적(正常的)인 (C-A) 개념(槪念)에 잘 적용된다는 것을 보여주었으며, 정상적(正常的)인 생육(生育)을 하고 있는 연초식물(煙草植物)에 있어서는 지상부(地上部)의 Carboxylate의 함량(含量)은 (C-A) 값과 거의 비슷함을 보여 주었다. 만약 식물체중(植物體中)에 $NH_4$함량(含量)이 상당히 많은 양(量)으로 존재(存在)한다면 $NH_4$를 분석(分析)하여 그 값을 (C-A)의 값을 계산하는데 사용해야 할것이다. Al은 가동성(可動性)이 매우 낮은 물질이므로 식물(植物)의 지상부(地上部)의 해(害)를 줄정도의 양(量)으로는 존재(存在)하지 않고 많은 양(量)이 뿌리의 내부나 그 주위에 존재(存在)한다. 식물체(植物體)가 Al을 많이 함유(含有)하고 있을 경우에도 (C-A) 값의 산출에 Al의 함량(含量)을을 고려해야 한다. An experiment with the tobacco plant was conducted in the pots. A sandy humic soil was used with 2 levels of pH, 3.5 and 5.8 with 2 kinds of anions, Cl as $NH_4Cl$ and $SO_4$ as $(NH_4)_2SO_4$, and with 4 levels of pF, 1.5, 2.0, 2.5, and 3.5. The pH-treatment created different N-forms; $NH_4$ at low pH(3.5) and $NO_3$ at high pH (5.8). The results are summarized as follows: 1. At low pH (3.5) with high concentration of $NH_4$ given as $NH_4Cl$, the high content of $NH_4$ and Cl in tobacco resulted in plants suffering from $NH_4$ and Cl toxicity as well as Mn toxicity. As a result of these toxicity, an extremly abnormal growth of tobacco was clearly appeared. In the tobacco grown at low pH with $NH_4$ given as $(NH_4)_2SO_4$, a large amount of the $NH_4$ uptake developed Mg and Ca deficiencies. $NH_4-N$, which had been applied to the soil of high pH (5.8), was almost completely transformed into $NO_3-N$ by nitrification and, on this low acidic soil, the plants were all healthy regardless of Cl or $SO_4$ added together with $NH_4-N$. However, dry matter production was higher and maturity faster when $SO_4$ was used as anion than when Cl was used. 2. High moisture content in soil, to some extent, is necessary for a good development and growth of the tobacco plant. Phosphate uptake seemed to be limited at higher moisture stress. The dry matter yield of tops and roots of tobacco were in the order of pF 1.8 > 2.1 > 2.6 > 3.6, respectively. 3. Data of chemical analysis and dry matter yields of tops and roots showed that the tobacco plant followed the normal (C-A) concept. In the normal growth of plants, the carboxylate content of tops was quite comparable to the estimated (C-A) values. If $NH_4$ content of plants remains in quite high quantities, it must be analysed and taken into consideration for the (C-A) calculation. Al is not transported toward tops in toxic amounts due to its high immobility, it mostly stay in or on the roots, probably due to precipitation as a aolt. When Al is present in high quantities, it has to be considered into the (C-A) calculation.

      • 土壤의 몇가지 理化學的 條件이 煙草의 生育 및 이온平衡에 미치는 影響

        金才正 충북대학교 엽연초연구소 1981 煙草硏究 Vol.8 No.-

        In order to study "effects of some physico- chemical conditions of the soil on growth and ionic balance of the tobacco plant", 4 parts of the experiment were carried out ; they were concerning effect of acidity (pH), moisture (pF) and anions (Cl and SO4) in soil on growth and ionic balance of tobacco (experiment 1), effect of ammonium and nitrate form of nitrogen in soil on growth and ionic balance of the tobacco plant (experment 2), effect of ammonium and nitrate form of nitrogen in strongly acidic soil on ionic balance of tobacco leaf (experiment 3), and effect of the various ground water levels in soil on transpiration and ionic balance of the tobacco plant (experiment 4). The results of these experiments are summarized as follows. 1.At low pH (3.5) with high concentration of NH4 given as NH4Cl, the high content of NH4 and Cl in tobacco resulted in plants suffering from NH4-and Cl - toxicity as well as Mn- toxicity. As a result of these toxicity, an extremly abnormal growth of tobacco was clearly appeared. In the tobacco grown at low pH with NH4 given as (NH4)2 SO4, a large amount of the NH4 uptake developed Mg- and Ca-deficiency. NH4-N, which had been applied to the soil of high pH (5.8), was almost completely transformed into NO3-N by nitrification and on this low acidic soil the plant were all healthy regardless of Cl or SO4 added together with NH4 -N. However, drymatter production was higher and maturity faster when SO4 used as anion compared to Cl. 2.High moisture content, to some extent, in soil is necessary for a good development and growth of the tobacco plant. Phosphate uptake seemed to be limitted at higher moisture stress. The dry matter yield of tops and roots of tobacco, respectively, were in the order of pF 1.8>2.1>2.6>3.6. 3.In the thickness and the weight per unit area of the fresh leaf, their values, respectively, was higher in NO3 treatment than in NH4 treatment and increased with increasing pF values of soil (pF 1.8 - 3.6). 4.In general the growth speed of tobacco leaf was faster in the order of pF 1.8>2.1>2.6>3.6 of soil. In the regime of pH 1.8- 2.6, the leaf growing of early stage was better in NH4 treatment than in NO3 treatment , but the leaf growing of latter stage was better in NO3 treatment than in NH4 treatment. In the pot of pF 3.6 that is short of available water to plant and lead to the temporary wilting point, the growing of plant was more restrained in NO3 treatment than in NH4 treatment through the whole growing period. 5.Judging from yield, size of leaf and stem of the tobacco plant grown on the pots with ground water levels, 15, 35, 55, 75, 95, 120 and 145cm, a good growth was observed on the pots with ground water levels, 55, 75 and 95 cm, especially the most active growth on the pot with 55 and 75cm. A morbid growth of tobacco was due to the unhealthy roots in the nearly water saturated soil in the pot with ground water levels 15 and 35 cm, and tobacco growth on the pot with ground water levels, 120 and 145 cm was inhibited because of water stress. 6.Under the vinylhouse conditions it was estimated that the relationship between dry matter production (P) and evapotranspiration ( We ) of tobacco was "P= 4 We " and coefficient "4" in this equation was characteristic for evapotranspiration of the tobacco plant. There was a tendency that evapotranspiration is increased with gradual growing of tobacco leaf and then slightly decreased at the maturing period of leaf. 7.The content of total inorganic cation (∑C) and anion (∑A) of tobacco increased with increasing soil moisture, respectively. Total inorganic cation content of tobacco grown on the NO3 pot was higher than that on the NH4 pot, but the content of total inorganic anion of tobacco was almost same in both NO3 pot and NH4 pot. 8.The (C-A) content, which was calculated from the content of total inorganic cation and total inorganic anion, was nearly constant without effect of moisture content (or variation of ground water levels) in soil. But the forms of nitrogen had a great effect on the (C-A) content. The normal (C-A) content per Kg dry matter for leaf of the tobacco plant in the NH4 pot was 1200 - 1300 me, for stem 750- 850 me and for root 550-650 me, and for leaf in the NO3 pot it was 1800- 2300 me, for stem 950- 1250 me and for root 850- 1000 me. Also the (C-A) content of leaf of tobacco grown in strongly acidic soil was 1200 - 1300 me in the NH4 pot and 1800- 2000me in the NO3 pot. 9.Generally the (C-A) content of leaf of tobacco grown in the NO3 treated soil was almost 2 times as much as that in NH4 treated soil. When the (C-A) content considered to be carboxylate content of tobacco, it is regarded that tobacco grown on soil with NO3 treatment would be better in combustibility and smoking taste than that with NH4 treatment . 10. K and Ca content of tobacco leaf was higher when Cl was applied as anion to soil compared to SO4. Addition of Cl to soil, which is one of the ways to increase K uptake to tobacco, not only interferes the growth of tobacco but also deteriorates the quality. In this respect, application of NO3-N will be an advisable method to increase K content together with SO4 or H2 PO4 and to decrease Cl content of tobacco leaf.

      • 幼若家兎의 長管骨 骨端成長板에 X 線照射가 미치는 影響에 關한 組織學的 硏究

        金在正,李弘鍵 고려대학교 의과대학 1975 고려대 의대 잡지 Vol.12 No.1

        The effect of X-irradiation upon the growth of long and flat bones has been of interest ever since the discovery of roentgen ray in 1895. The roentgen ray has been applied very effectively for the diagnosis and treatment of diseases but complications such as the stunting of bone growth, fracture, radiation osteitis, decalcification, bony necrosis and very rarely malignant tumors of bone have been reported to occur in the course of roentgen ray therapy. Since the appearance of Perthes’ report(1903) which first demonstrated gross underdevelopment of osseous structures following X-irradiation, numerous investigations have been recorded on the subject of the effect of X-irradiation on the skeletal system. From a careful review of all the literatures available, it appears that there has been a conspicuous lack of clearness about the amount of radiation doses necessary to inhibit the growth of bone, the duration of the effect and the accompanying histological changes. The author, therefore, has undertaken this study in order to obtain the detailed histological changes of the epiphyseal plates in the growing rabbits following X-irradiation. Experimental method: One handred rabbits weighing 500 to 700 gm were used all experiments. These were subdivided into Group Ⅰ, Ⅱ and Ⅲ according to the method of X-irradiation to the right knee: Group Ⅰ: A total dose of 3000 R was given in 6 increments within 3 weeks. Group Ⅱ: A total dose of 3000 R was given in 3 increments within 3 weeks. Group Ⅲ: A single dose of 3000 R was given at one setting. The irradiated rabbits were sacrificed at the 1st, 2nd, 3rd, 4th, 6th, and 8th week respectively and the histological observations of the epiphyseal plates of distal femurs were made. Major findings observed are summarized as follows: 1. The distortion of cartilage columns and the atrophy of epiphyseal plates following X-irradiation were found in each group. 2. In Group Ⅰ and Ⅱ, the damaged epiphyseal plates following X-irradiation began to regenerate at the 4th week and returned almost to normal feature at the 6th-8th week. On the other hand, in Group Ⅲ the calcified cells remained persistently at the zone of developing trabeculae of metaphysic as well as at the zone of calcifing cartilage and therefore normal ossification process of the trabeculae was disturbed and delayed. 3. Bony change at the zone of trabeculae following X-irradiation was mild in Group Ⅰ, moderate in Group Ⅱ and most severe in Group Ⅲ. 4. Regeneration process of the damaged epiphyseal plates began early in Group Ⅰ and it was most retarded in group Ⅲ. 5. In group Ⅲ, there were fatty infiltration and few cellular elements in the bone marrow cavity, and myelofibrosis of the marrow tissue appeared. Therefore, it was apparent that the regeneration process was retarded. 6. X-irradiation in divided doses causes less damage to the epiphyseal plate and accordingly regenerative process was so much faster.

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