살조개, Protothaca jedoensis 초기치패의 저질 및 먹이생물별 성장

김정(Jung KIM),추부관(Bu Gwan Chu),장선익(Sun Ik JANG),한종석(Jong Seok HAN),라성주(Sung Ju RHA),안윤근(Yun Keun AN),윤호섭(Ho Seop YOON),최상덕(Sang Duk CHOI) 전남대학교 수산과학연구소 2011 수산과학연구소논문집 Vol.19 No.-

In order to obtain the basic information for artificial seedling production of venus clam, Protothaca jedoensis, growth and survival rate were investigated by substratum composition on spat collection, and species and concentration of food organisms during spat stage. The optimal substratum for spat collection was the sand size (Ø0.5-2 ㎜) showing that the highest growth and survival rate of spat were 493.2±0.4 ㎛, 13.0±0.9 %, respectively. As for spat rearing by four species of food organism (phytoplankton), Isochrysis galbana, Chaetoceros calcitrans, Monochrysis lutheri, and Pavlova lutheri were used singularly and the mixture of the four species was used. The highest growth and survival rates were observed in the Isochrysis galbana (1644.2±0.8 ㎛, 79.7±2.3 %), and followed by mixture group, Chaetoceros calcitrans, Monochrysis lutheri, and Pavlova lutheri. In the concentration of the four mixed food organisms on spat rearing, the highest growth and survival rates were observed in the concentration of 1×104 cells/㎖ (1637.0±1.0 ㎛, 75.0±0.9 %), and followed by 2×104 cells/㎖, 4×104 cells/㎖, and 0.5×104 cells/㎖. 살조개, Protothaca jedoensis의 인공종묘생산 기술개발을 목적으로 생물학적 기초자료를 얻고자 저질입도별 채묘 및 치패사육에서 먹이생물의 종류와 농도에 따른 성장과 생존율을 조사하였다. 저질입도별 채묘에서 최적 채묘기질은 극조립사(Ø0.5-2㎜)로 가장 높은 성장(493.2±0.4㎛)과 생존율(13.0±0.9%)을 보였다. 먹이생물별 치패사육에서 Isochrysis galbana가 가장 높은 성장(1644.0±0.8㎛)과 생존율(79.7±2.3%)을 보였으며, 혼합구, Chaetoceros calcitrans, Monochrysis lutheri, Pavlova lutheri 순으로 나타났다. 먹이생물(혼합) 농도별 치패사육에서 1×104cells/㎖가 가장 높은 성장((1637.0±1.0㎛)과 생존율(75.0±0.9%)을 보였으며, 2×104cells/㎖, 4×104cells/㎖, 0.5×104cells/㎖ 순으로 나타났다.

단색 분광기 회절격자의 정밀 구동 기구에 관한 연구

김대석(Dae-Suk Kim),최기봉(Kee-Bong Choi),김수현(Soo-Hyun Kim),곽윤근(Yoon Keun Kwak) Korean Society for Precision Engineering 1997 한국정밀공학회지 Vol.14 No.11

가변 형상 무한 궤도 차량의 성능 해석 및 설계

김한호(Han ho Kim),곽윤근(Yoon Keun Kwak) 한국자동차공학회 2001 한국 자동차공학회논문집 Vol.9 No.1

제주도 지역에서 귤응애에 의한 직업성천식

김윤근(Yoon Keun Kim) 대한천식알레르기학회 1999 천식 및 알레르기 Vol.19 No.6

N/A

천식 환아의 형제에서 흡입성 항원에 의한 피부반응도 및 기관지과민성과 염색체 11q13 유전형 사이의 연관성

김윤근(Yoon Keun Kim),손지웅(Jee Wong Son),조상헌(Sang Heon Cho),이명현(Myung Hyun Lee),고영률(Young Yull Koh),민경업(Kyung Up Min),김유영(You Young Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.4

Background: Increased IgE antibody responses to inhalant allergens and bronchial hyperres- ponsiveness are important phenotypes in development of asthma. Although heredity reported to be important in expression of these phenotypes in twin and family studies, genetic factor(s) controlling these phenotypes is unknown. Objective: To evaluate whether genetic factor in chromosome 11q13 may control the expression of IgE responses to common inhalant allergens and bronchial hyperresponsiveness, linkage analysis between these phenotypes and gene marker of chromosome 11q13 was investigated. Material and methods: The phenotyping and genotyping using microsatellite marker (D11S97) were performed in 77 probands with bronchial asthma and 80 their sibs. The linkage analysis between these phenotypes and the genotype was evaluated by affected or quantitative trait locus (QTL) sib-pair analysis. Results: Positive skin test responses to inhalant allergens were 55/77(71.4%) in probands and 44/79(55.6%) in sibs, respectively. Positive bronchial provocation test responses to methacholine were 27/61(44.3%) in sibs, geometric mean of PC20-methacholine were 5.2 mg/ ml in probands and 39.4 mg/ml in sibs, respectively, and slope of dose response curve(mean+- SE, %/mg/ml) were 11.3 +- 3.22 in probands and 1.97 +- 0.5 in sibs, respectively. Of 34 sib-pairs with positive skin test responses to allergens, two D11S97 alleles were shared by 21(61.8% ) sib -pairs, one allele by 11(32.3% ) sib-pairs, and no identical allele by two(5.9% ) sib-pairs. In affected sib-pairs, sharing rate of the alleles was 77.9%, which indicates linkage of the phenotype and genotype(p<0.001). Of 25 sib-pairs with bronchial hyperresponsiveness to methacholine, two D11S97 alleles were shared by seven(28%) sib-pairs, one allele by 11(44%) sib-pairs, and no identical allele by seven(28% ) sib-pairs. In affected sib-pairs, sharing rate of the alleles was 50%, which indicates no linkage between the phenotype and genotype(p) 0.05). Differences of geometric value(mean +- SE) of PC-methacholine and slope of dose response curve(mean +- SE, %/mg/ml) were 1.11+- 0.17 and 8.33+- 3.35 in sib-pairs sharing two alleles, respectively, 0.99 +- 0.14 and 14.27+-5.75 in sib-pairs sharing one allele, respectively, and 0.57+-0.13 and 3.64+-1.62 in sib-pairs sharing no allele, respectively. There was no difference of the above values among the three groups. Conclusion: The expression of skin reactivity to common inhalant allergens was linked to gene marker of chromosome 11q13, not with bronchial responsiveness to methacholine.

영유아천명후 기관지천식으로의 이행에 미치는 부모이 아토피와 기관지과민성의 영향

김윤근(Yoon Keun Kim),고영률(Young Yull Koh),이명현(Muyng Hyun Lee),정진화(Jin Hwa Jung),지영구(Young Koo Jee),조상헌(Sang Heon Cho),민경업(Kyung Up Min),김유영(You Young Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.4

Background: Many young children suffer from wheezing illness during viral respiratory infection, and some of them experience wheezing many years later and ultimately develop bronchial asthma. It is not clear whether atopy or bronchial hyperresponsiveness in the family is a significant risk factor for asthma in this clinical setting. Objective : To examine the genetic basis for the development of asthma after early childhood wheezing. Materials and methods : A measurement of serum total IgE concentration, skin prick test to common inhalant allergens, and methacholine bronchial provocation test were performed in 29 asthmatic children and their parents, and 22 non-asthmatic children with the past history of wheezing illness during the first three years of age and their parents. A questionnaire was performed to assess the presence of asthma and allergic rhinitis in the parents. Results : Positive skin test response to common inhalant allergens was more prevalent in asthmatics than in non-asthmatics(67.8% vs. 27.2%). Serum total IgE concentration was significantly higher in asthmatics than in non-asthmatics(geometric mean: 173 vs. 83 IU/ ml). Positive skin test response to comman inhalant allergens was more prevalent in parents of asthmatics than in thoae of non-asthmatics(51.7% vs. 25.0%), but serum total IgE level was not different between the two groups(geometric mean: 132 vs. 120 IU/ml). Positive rate of methacholine bronchial provocation test, geometric mean of PC20-methacholine, and BR index were not different between the parents of asthmatics and non-asthmatics (18.1% vs. 13.9%; 164 vs. 180 mg/ml; 1.154+-0.077 vs. 1.055+-0.068, respectively). Conclusion : It is suggested that personal atopy is important in the development of asthma after early childhood wheezing, and parental atopy rather than bronchial hyperresponsiveness is a risk factor for the development of childhood asthma in this clinical setting.

호염기구 히스타민 유리능의 가족적 발현에 관한 연구

김윤근(Yoon Keun Kim),정진화(Jin Hwa Jung),김창근(Chang Keun Kim),고영률(Young Yull Koh),조상헌(Sang Heon Cho),민경업(Kyung Up Min),김유영(You Young Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.4

Backgronnd: Bronchial asthma is a complex genetic disorder. Although serum IgE level and bronchial hyperresponsiveness are well known to be under genetic control, the influence of genetic factors on basophil releasability has been seldom studied. Objective : The present study was carried out to investigate whether genetic factors may influence the basophil histamine releasability. Materials and methods : We studied 50 children, 32 with atopic asthma (AA) and 18 normal control (NC), and their parents. Suspensions of leukocytes were isolated and stimulated with Ca ionophore and anti-IgE antibody. Then, histamine in the supernatant was as-sayed by an automated fluorometric analyzer. Results : Among the probands, AA children had a significantly higher anti-IgE induced histamine release than NC children. In contrast, Ca ionophore-induced histamine release was similar between the two groups. Ca ionophore-induced or anti-IgE-induced histamine release was not significantly different between parents of AA children and those of NC children. However, the maximal histamine release by Ca ionophore in parents had a significant correlation with that of probands, whereas the values by anti-IgE were not correlated between probands and their parents. Conclusion : We confirmed that basophils from patients with atopic asthma are characterized by a specific increase in IgE-mediated histamine release. The significant correlation of Ca ionophore-induced maximal histamine release between children and their parents suggests that genetic factors may play an important role in the control of non-IgE-mediated relessability from basophils.

기관지천식의 가족적인 발현 및 아토피와 기관지과민성의 유전적인 역할

김윤근,이상록,손지웅,조상헌,이명현,고영률,민경업,김유영 (Yoon Keun Kim,Sang Rok Lee,Jee Woong Son,Sang Heon Cho,Myung Hyun Lee,Young Yoll Koh,Kyung Up Min,You Young Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.1

Background: Familial aggregation of the phenotypes can be caused by common environmental and genetic factors, but there has been no family study on familial aggregation of the bronchial asthma, and genetic role of atopy and bronchial responsiveness in the development of asthma in Korean families. Objective . We did family study to evaluate the familial aggregation of bronchial asthma, and the genetic role of atopy and bronchial responsiveness in the development of asthma. Materials and Methods '. Questionnaire, serum total IgE level, skin prick test with 10 common aeroallergens, and bronchial responsiveness to methacholine were performed in 154 parents of atopic asthmatics, 72 parents of atopic control, and 65 parents of non-atopic control. Resulta '. Bronchial asthma was more prevalent in parents of atopic asthmatics(7.1% ) than in parents of non-atopic control(0% ). Geometric mean of serum total IgE level was not different among parents of atopic asthmatics, atopic control, and non-atopic control(2.03+0.06, 2.10 +0.07, and 1.89 +0.09 IU/ml). Positive rates of skin prick test to 10 common aeroallergens were more prevalent in parents of atopic asthmatics(43.0% ) and atopic control(43.0% ) than in parents of non-atopic control(27.8%). Prevalence of bronchial hyperresponsiveness to methacholine was more prevalent in parents of atopic asthmatics(17.0% ) than in parents of atopic control(7.2%) and non-atopic control(1.5%), and slope of dose-response curve was more increased in parents of atopic asthmatics(11.0+ 1.5) than in parents of atopic control and non-atopic control(4.8+ 0.7 and 3.0+ 0.5). Conclusion . Bronchial asthma runs in Korean families, and genetic role of atopy and bronchial responsiveness may be important in the development of asthma.

약물 알레르기의 최신 지견

김윤근(Yoon Keun Kim) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.6

N/A

천식 환아의 형제에서 혈청 총 IgE 농도와 염색체 11q13 유전형 사이의 연관성

김윤근,손지웅,조상헌,이명현,고영률,민경업,김유영 ( Yoon Keun Kim,Jee Wong Son,Sang Heon Cho,Myung Hyun Lee,Young Yull Koh,Kyung Up Min,You Young Kim ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Backgrmcnd; It is known that total serum IgE levels closely corrleate with prevaience of asthma regardless of atopic status. Although heredity is reported to be important in expression of total serum IgE in twin studies, genetic factor controlling this phenotype is controversial. Objective .' To evaluate whether genetic factor in chromosome 1 1q13 may control the expres- sion of tatal serum IgE level, linkage analysis between this phenotype and gene marker of chromosome 11q13 was investigated. Material and method: Total serum IgE level and the genotype of chromosome 11q13 with microsatellite marker (D11597) was determined in 73 probands of asthmatic chiMren and 76 their sibs. Statistical significance of linkage was evaluated by affected and quantitative trait locus (QTL) sib-pair analysis. Result: In 20 affected sib-pairs with total serum IgE level higher than 305 IU/ml (geometric mean plus two folds SD in 53 normal controls), two D11S97 alleles were shared by ten sib- pairs, one allele by nine sib-pairs, and no allele by one sib-pairs. Sharing rate of the alleles in affect,ed sib-pairs, was 72.5%, which indicates linkage of the phenotype and genotype (x=4. 27, p=0.03). In 35 sib-pairs with total serum IgE level higher than 170 IU/ml (geometric mean plus one fold SD in 53 normal controls), two D11S97 alleles were shared by 16 sib-pairs, one allele by 15 sib-pairs, and no allele by four sib-pairs. The shar ing rate of the alleles in affected sibpairs, was 67.1%, which indicates linkage of the phenotype and the genotype(x=4. 24, p=0.03). Difference of geometric value of total serum IgE levels between probands and their sibs wa,s smaller in 32 sib-pairs sharing two alleles than in 32 those sharing one allele and 12 those with no identical allele (0.45+0.07 vs. 0.52+0.07 vs. 0.89 +0.21). Conclasion .' The expression of total serum IgE level was linked to gene marker of chromosome 11q13.