단백질원에 따른 흰다리새우 (Litopenaeus vannamei)의 성장효과

김수경,김은지,서형철,장인권,김수경 한국수산과학회 양식분과 2012 한국수산과학회 양식분과 학술대회 Vol.2012 No.6

흰다리새우 유생의 저염분 순치방법에 따 른 생화학적 특성변화

김수경,심나영,장진우,전제천,김수경,신윤경 한국환경생물학회 2017 환경생물 : 환경생물학회지 Vol.35 No.1

This study focused on the physiological change of the shrimp, Litopenaeus vannamei postlarvae 15 stages, under different acclimation methods up to the endpoint of 4 practical salinity unit (psu). Besides using sea water as the control, two acclimation methods, fast acclimation (50% salinity reduction every 8 hours) and slow acclimation (50% salinity reduction every day), were adapted. Results show that the survival rate, glucose and blood uric nitrogen of each group were not significantly different. However, the ion profile differed according to the acclimation methods. Magnesium and sodium of shrimps acclimated to low salinity in both the methods, showed lower concentration than shrimps at 32 psu sea water. Especially, Na+ concentration, which directly influences the osmolality of shrimp, decreased sharply in the fast acclimated group during the first eight hours (from 32 psu to 16 psu). To reduce acclimation stress, it is recommended to take more than eight hours during the first step for reducing the salinity.

바이오플락 탈질수가 어린 흰다리새우, Litopenaeus vannamei의 생존율 및 생리특성에 미치는 영향

김수경,장진우,조영록,김준환,김수경 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.2

This study investigates the effect of denitrified biofloc water on changes in the water quality parameters and the physiological characteristics of shrimps. Biofloc rearing water contains a large number of microorganisms and can rapidly stabilize the water quality and energy saving if reusable due to high water temperatures. Rearing water contain floating bacteria with both aerobic and anaerobic bacteria. Therefore, when the carbon source is added in limited air supply, the anaerobic state is activated and the denitrification process is possible. In this study, the denitrification water had the following properties: ammonia (6.9 mg L-1), nitrite (0.3 mg L-1), nitrate concentration (9.2 mg L-1), high pH (8.42) and alkalinity (590 mg L-1). The experimental group consisted of seawater (SW, control), a mixture of Seawater and denitrified biofloc water (DNW) in the ratio of 3:1, 1:1 and DNW only. All experiments were done in triplicate. As a result, the survival rate never changed even when 100% of the denitrification water was utilized. However, a body fluid analysis showed that creatine and BUN were increased due to index of stress and the tissue damage resulting from the high denitrified water content. Body fluid ions (Na+, K+, and Cl-) significantly decreased as the denitrified water content increased. It was recommended that the denitrification water be mixed with a certain ratio (less than 50%) in the future as it may affect the osmotic pressure control in shrimps. 안정된 바이오플락 사육수에는 대량의 미생물들이 존재하고 있으며 사육수온이 높아 재사용이 가능할 경우 빠른 수질안정화 및 에너지 절약을 할 수 있다. 바이오플락 사육수 내 부유하고 있는 자가 및 타가 영양세균은 호기성과 혐기성 세균을 모두 포함하고 있어 탄소원을 넣고 산소를 공급하지 않는 혐기성 상태로 만들면 탈질과정이 가능 하다. 본 연구에서 바이오플락 탈질수의 특성은 암모니아(6.9 mg L-1), 아질산 (0.3 mg L-1), 질산농도 (9.2 mg L-1), 높은 pH (8.42), alkalinity (590 mg L-1)였으며 이 탈질수를 첨가한 사육수의 물리적 환경 변화가 어린새우의 생존 및 생리적 특성에 미치는 영향을 조사하였다. 그 결과 탈질수를 100% 사용하여도 생존율의 변화를 보이지 않았으나 혈림프를 포함한 체액 분석결과 탈질수 혼합에 의한 조직손상 및 스트레스 지표인 크레아틴, 혈중 요소성 질소의 증가가 관찰되었고 탈질수 혼합비율이 높을수록 새우 체내 이온(Na+, K+, Cl-)의 농도가 유의적으로 감소하여 향후 삼투압조절에 영향을 미칠 수 있는 것으로 나타남에 따라 탈질수를 일정비율로 (50% 미만) 혼합하여 사용하는 것이 바람직한 것으로 사료된다.

주부 김수경

김수경 충북연구원 2009 앞,뒤 바라보다 Vol.- No.2

해수 저염분 순치과정에서 먹이섭취가 흰다리새우, Litopenaeus vannamei 유생에 미치는 영향

김수경,심나영,조지현,김종현,김수경 한국환경생물학회 2018 환경생물 : 환경생물학회지 Vol.36 No.3

저염분 순치기간에 먹이를 섭취함으로서 얻을 수 있는 초기 유생의 생리적 변화는 다음과 같이 요약할 수 있다. 하루 동안의 순치기간에 염분농도에 따른 유생의 생존율은 차이를 보였지만 각 염분구간별 먹이섭취 유무에 따른 생존효과는 유사하였다. 그러나 먹이섭취 시 유생이 에너지원으로 활용할 수 있는 cholesterol, triglyceride의 증가가 있었으며 삼투압에 영향을 미칠 수 있는 glucose 농도가 유의적으로 증가하였다. 또한 저염분 순치기간 동안에 스트레스, 조직손상 및 대사작용의 지표가 되는 BUN과 creatine의 감소가 있었다. 먹이섭취로 인한 소화효소의 활성이 먹이를 섭취한 실험구 모두에서 증가하였다. 그러므로 유생발달과 함께 중요한 소화, 순환, 생식기관이 형성되며 탈피 성장을 위한 신진대사가 빠르게 진행되는 유생시기에 저염분 순치가 효율적으로 이루어져 향후 지속적인 성장을 유도하기 위해서는 먹이를 섭취하는 것이 더 효과적인 것으로 판단되었다. This study focused on the effects of feeding on postlarvae of shrimp, Litopenaeus vannamei, during the identified acclimation time to low salinity. A total of 5 different salinity groups with or without feeding (32, 24, 16, 8, and 2 psu, 1 liter, triplicates) were prepared, and 30 shrimp were settled at PL21 (postlarvae) and placed in each group. After 24 hours of the experimentation process, the survival rate of the fed and starved groups was observed to be lower in the 2 psu group compared to other salinity groups, with the rate of 86.6% and 81.1%, respectively. The condition index of glucose and triglyceride, which are important factors for osmoregulation and as energy sources, was 4.2-7.6 times and 2.7-3.4 times higher in the fed groups than the starved groups at all the levels of salinities. The creatine level increased by 1.1-1.5 times in the starved groups as compared to the fed groups. Likewise, the activity of all the digestive enzymes like, lipase, α-amylase, trypsin, and alkaline protease were clearly higher in the fed groups (ANOVA, p<0.05). Apparently, it was observed that feeding is effective for the postlarvae of shrimp, which shows a characteristic fast metabolism and larval development, during the acclimation period to low salinity.

노인 및 치매 환자의 회상 치료 효과

김수경 대한작업치료학회 2003 대한작업치료학회지 Vol.11 No.2

This article presents an overview of the reminiscence concept and the results of intervention studies in reminiscence therapy on the elderly and the dementia. Reminiscence is thinking and talking about one’s past. it is a composit mental process with involves psychological, emotional, and social interaction aspects. Reminiscence therapy contribute to psychological adaptation by improving of self-esteem, self-integration and life satisfaction and increasing emotional support, activity of daily living adaptation and coping skill on the elderly and the dementia.Depression, anxiety of death, disability and pain were decreased through reminiscence therapy.Especially, communication was improved and behavioral problem was reduced in patients with dementia. Reminiscence therapy are effective method on psychosocial aspects of the elderly with or without disability and it expected to have potential as a therapeutic activity in the field of occupational therapy.

상투성의 전복, 구체성의 미학 : 작사가 양인자론 a study of songwriter, Yang In-Ja

김수경 이화여자대학교 이화어문학회 2003 이화어문논집 Vol.21 No.-

This article investigates the characteristics of the song written by Yang In-Ja who made Cho Yong-pil more popular in late 1980s. Her songs are not typical or not full of cliches. They are very beautiful, and people in that decade loved her songs. The characteristics of her song are, 1) The optimistic insight over the life: In her words of songs, we can find the person who never falls in despair or sorrow even after heart-broken separation. 2) The sense of details : The power of expression of her songs is the concrete, detailed description about when, where, how they met and took apart from each other. 3) The starting point of "ballard" in Korean style : The majority of her songs are ballard, and her ballard had a lot of affect on the ballard in 1990s. There are so many things that made one song: sound, melody, words, singer, back-musics and even dances. I think, the words are one of the best important thing in them, and words of Yang In-Ja verified, demonstrated it.

남성성과 여성성과 대립으로 본 헌화가

김수경 이화여자대학교 이화어문학회 1999 이화어문논집 Vol.17 No.-

This thesis is on the song "Hunwha-ga"(song of giving flower to woman named Suro) and attach story about Suro. In the song/story, there are one beautiful woman: Suro and three men; an old man, the dragon and her husband Soonjung. These three men have something in common with one. That, they adore her. But they love/adore/respect her in different way. " old man"ave her the flower and song. It is very elegant way of affection. He represents god or god-like, so, Suro is so beautiful that even god also had affection for her. But that god showed himself not as a handsome, young guy but as a plain old man. This setting makes the song/story not too lascivious or sensual, "e dragon"arried her away to the deep blue sea by force. It is very brutal way of love. He represents an ordinary man who would stop at nothing to gain a beautiful woman. So he made himself open to severe criticism, he took her back eventually. "r husband Soonjung"ust kept still when a strange old man gave his wife a flower, or when the dragon kidnapped her. It is very passive way of love. He represents 'her' purity and chastity because his name 'Soonjung' exactly means that. The point of the song/story is that the woman named Suro is so beautiful that god and the monster as well as an ordinary man cannot help loving her. Therefore, the song/story accentuated the feminity but made the masculinity weakened.

Synthesis and characterization of Dextran-Spermine-gCB polymer as a efficient targeted gene carrier

김수경,박경민,최지숙,김기문,김원종 한국고분자학회 2009 한국고분자학회 학술대회 연구논문 초록집 Vol.2009 No.10

The Changes of Cyclic AMP Content by Opiates in Chronic Haloperidol Treated Mouse Striatum

김수경,Kim, Soo-Kyung The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.1

Opioid수용체는 adenylate cyclase의 활성을 억제하므로써 cyclic AMP의 양을 감소시킨다. 본 연구에서는 striatum에서 dopamine과 opioid 신경전달계의 상호관계를 알아보고자 haloperidol(750ug/kg)을 10일간 복강내 투여하여 dopaminergic pathway를 차단시킨후 mouse striatum에서 선택적 opioid ${\mu},\;{\gamma}\;{\kappa}$ 수용체 agonist들에 의해 축적되는 cAMP양을 측정하여 본 결과, haloperidol단독투여에 의해서 cAMP는 유의한 증가를 나타내었으며, haloperidol 장기투여된 mouse striatum 에서 morphine(20mg/kg), DAGO(5Oug/kg), DPDPE(50ug/kg), U5O,488H (500ug/kg)투여에 의해서 haloperidol에 의한 cAMP 증가는 억제되었으며, 정상 mouse에 투여된 morphine, DAGO, DPDPE, U5O,488H에 비해서는 DAGO, DPDPE 투여군에서 증가를 나타내었다. Haloperidol장기투여로 인한 morphine, DAGO, DPDPE, U5O,488H의 영향은 naloxone에 의해서 morphine과 U5O, 488H투여군에서 길항되었으며 정상 mouse에 투여된 morphine, DAGO, DPDPE, U5O,488H에 의한 cAMP의 감소는 naloxone에 의하여 모든 실험군에서 길항되었다. 이상의 결과로 보아 dopaminergic denervation시 mouse striatum에서 ${\mu},\;{\gamma},\;{\kappa}$효현제에 의하여 축적되는 cAMP양은 ${\kappa}$수용체 효현제인 U5O,488H에서 가장 현저한 감소를 보여 각 수용체의 활성화정도는 변화되며, 그중에서 ${\kappa}$수용체는 그 기능이 가장 보존되고 있음을 알 수 있었다. Cyclic adenosine 3'5'-monophosphate (cyclic AMP) has been frequently accepted as an intracellular messenger for receptor-mediated action of opioids. In this experiment, it was designed to determine the interaction of dopaminergic and opioidergic system in the mouse striatum in normal and chronic haloperidol treated groups. Haloperidol 750ug/kg I.P. for 10 days was performed for dopamine denervation. The morphine, DAGO, DPDPE, and U5O,488H inhibited the increase of haloperidol-induced cyclic AMP content in chronic haloperidol treated mouse striatum. The inhibition of DAGO and DPDPE showed significant increase compared to normal mouse striatum. Naloxone showed antagonistic effect on the morphine and U5O,488H in chronic haloperidol treated group, and showed antagonistic effect on morphine, DAGO, DPDPE, and U5O, 488H in normal mouse striatum. These findings support that there is a functional interrelationship of dopaminergic and opioidergic pathway in the striatum. This result provides an evidence that following destruction of striatal dopaminergic neuron, there are some changes of cAMP content on the ${\mu},\;{\gamma},\;and\;{\kappa}$ opioid receptor, but the ${\kappa}$ opioid receptor still has its function.