卵巢畸形腫의 病理組織學的 檢索

梁大石,金春元 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.1

To many the term ovarian dermoid cyst denotes merely a ball of hair invested by amantle of ectodermal tissue occasionally supporting one or more teeth. Careful examination, however, frequently reveals a variety of tissues including bone, cartilage, smooth muscle, fat, and so forth. Benign custic teratomas are believed to be associated with a high includence of complications; it appears, however, that this concept is based on clinical impressions handed down to us. The laterality, ago incidence, size and microscopic description of these tumors are discussed in considerable detail. Brief mention also is made regarding the incidence of struma ovarili, pregnancy, torsion and coincidental lesions found in association with ovarian teratomas. A histopathologic study was made on 137 teratomas of ovary that were obtained during a period of 8 years and 10 months, from May 1972 to Feb, 1981. at the department of Clinical Pathology, College of Medicine, Han Yang University. The following results are obtained 1. Begin cystic teratomas are 96.5%, maligant teratomas are 2.1% of which shows 1.4% squamous carcinoma and 0.7% of adenocarcinoma and struma ovaril are 1.4%. 2. Age distribution is 71.9% in 2nd to 3rd decades and mean ageis 32 years. The youngest one is 4 years and the oldest is 68 years. 3. No site predilection was identified with bilaterality in 11.3% of cases. 4. The overage size of the tumor is 9.84cm in diameter and 80.5% of cases are between 5cm to 14cm in cross diameter. 5. Clinical symptom is developed within 1 year in 60% of case. 6. The main symptoms are abdomnal pain, addominal mass and menstrual distrubance, in order of frequency any they are 50%, 41.7% and 10.6%, respectively. 7. Associated lesions are pregancy, cervicitis, squamous metaplasia and uterine myoma, serous cystadenoma and proteinuria and they are 20%, 10.6%, 5.3% and 24.2%, respectively. 8. The complications are tortion(15.9%), rupture(2.3%) and infection(0.8%). 9. The componets are skin and its appendage are 97% and 87.9% respectively. The majority is ectodermal origin tissue.

貯藏方法에 따른 家兎血淸 Transaminase 및 Alkaline Phosphatase 活成値藥動

金春元 우석대학교 의과대학 1967 우석의대잡지 Vol.4 No.1

This is an experimental study to evaluate the changes and stability of rabbit's serum transaminase and alkaline phosphatase activities in varying temperatures. Four adult male rabbits were artificially intoxicated by subcutaneous injection of 30% carbon tetracholoride olive oil. Blood samples were drown at 12 hours after the injection and sera were seperated form the blood within 3 hours. Among the total specimens, each 33 were stored respectively at room temperature (20.4˚±4.6℃), in refrigerator (4˚∼8℃) and in deep freezed(-18˚∼20℃). Then activities of serum glutamic oxalacetic transaminase(SGOT), serum glutamic pyruvic transaminase (SGOT) and alkaline phosphatase(ALP) were measured periodically up to 34 days. The results were as follows. 1. Room tempereture: Activities of SGOT, SGPT and ALP were preserved up to 1,9 and 27 days respectively. 2. Refrigerator: Activities of SGOT, SGPT and ALP were preserved up to 4,14 and 19 days respectively. 3. Deep freezer: Activities of SGOT, SGPT and ALP were preserved up to 24,9 and 19 days respectively.

정상 한국 성인의 혈청 Apoprotein A-Ⅰ치에 관한 연구

정용호,김춘원 한양대학교 의과대학 1983 한양의대 학술지 Vol.3 No.1

In recent years, much attention has been focused on high density lipoproteins in the predictor of atherosclerosis, HDL is approximately 50% protein by weight and 70% of this component is apoprotein A-I. Since theprotein moiety of HDL is the most important determinant of its compositional and structural stability, attention has turned to the estimation of HDL protein rather than cholesterol as an index of plasma HDL-concentration. Apoprotein A-I, themajor HDL protein, has been most intensively investigated in this regard. The plasma level of this protein is highly positively correlated with HDL-cholesterol, and consequently its measurement should provide a useful addition to HDL-cholesterol as a predictor of coronary heart disease. Author measured the value of serum apoprotein A-I in normal korean adult. Sera were obtained for 65 korean male and 55 female. The results were summeralized as follows: 1. The mean value of serum apoprotein A-I in normal korean male and female were 148.9±26.3mg6dl, 152.727.8mg/dl, respectively and its average were 150.4±26.2mg/dl. 2. Sex and age difference showed no statistical significance in the mean value of serum apoprotein A-I.

正常韓國人의 血淸Prolactin値에 關한 硏究

李季淑,金春元 한양대학교 의과대학 1983 한양의대 학술지 Vol.3 No.1

Nowadays, human prolactin is well estabished as a separate entity from pituitary growth hormone by a variety of biologic, chemical, immunologic and clinical characteristics. Specific and sensitive radioimmunoassay have been developed by several laboratories and this development has afforded an opportunity to study the prolactin under the physiologic and pathologic events. Author was determined the serum prolactin in 185 healthy Korean males and females according to age and sex differences by radioimmunoassay. The results were as follows: 1. The normal value (mean±1 SD) of serum prolactin was 10.8±5.3 ng/mL. 2. The normal values (mean±1 SD) in males and females were 10.0±4.8 ng/mL and 11.7±5.7 ng/mL, respectively. The difference of normal values of serum prolactin between males and females were significantly higher in females than in males. 3. The normal values of serun prolactin in females were in decreasing order to froth, first, third, fifth and second decades.

火傷 및 火傷後 Prednisolone 長期投與가「마우스」臟器의 電解質에 미치는 影響에 關한 實驗的 硏究

金春元,白承龍 고려대학교 의과대학 1973 고려대 의대 잡지 Vol.10 No.1

One of the most perplexing subjects in clinical physiology is fluid and electrolyte metabolism. It has long been recognized that deprivations, losses, and abnormalities of distribution affecting water and inorganic substances in the body are extreme importance in many clinical conditions. Author has attempted an experimental study to clarify the changes of tissue electrolyte contents after burn and prednisolone injection after burn. 510 male S.M. mice, weighing about 15~20gm were selected and divided into 4 groups as follows; normal control group, prednisolone injection group, burn group and prednisolone injection after burn group. The contents of Na, K and Cl in unit weight of the kidney, spleen and liver were measured after 4 hours, 1, 2, 3, 5, 7, and 10 days of experimental treatment. The result obtained are summarized as follows: 1. Electrolyte contents in unit weight of each organ of normal control group were as follows: Kidney: Na: 78.7±6.5, K: 76.8±5.8, Cl: 73.0±4.8 Spleen: Na: 44.4±2.4, K: 116.7±9.3, Cl: 88.9±5.8 Liver: Na: 40.3±2.7, K: 87.7±7.6, Cl: 44.0±2.4 The Na content was highest in the kidney and the K and Cl were highest in the spleen, while the Na and Cl contents in the liver were lowest. 2. In the spleen and liver of burn group, marked increase of Na and marked decrease of K was noticed throughout the course. Cl was moderately decreased only in the spleen. 3. There was no significant difference of electrolytes change in the spleen and lover between 10% and 20% burn of second degree. 4. In prednisolone injection after burn group, normal recovery of electrolytes change in the spleen and liver were delayed by prednisolone injection. 5. Both in burn and prednisolone injection after burn groups, tissue electrolytes change were not remarkable in the kidney on the contrary of marked change in the spleen and liver.

가토 결막세포내에서의 Chlamydia trachomatis의 구조에 관한 전자현미경적 연구

최태열,이맹복,김춘원 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

As human pathogens, the organism of C. trachomatis cause a number of diseases. Primarily these are diseases of the eye (tranchoma and inclusion conjunctivitis) and of the urogenital tract (lymphogranuloma venerum (LGV), urethritis, and cervicititis). The Chlamydiae are non motile, gram negative organism that sharing a similar morphology, and a common group antigen. They og through a unique developmental cycle within the cytoplasm of their host's cells. The Chlamydiae are prokaryotic obligate parasites of eukaryotic cells. Chlamydiae have two morphological forms-elementary body(EB) and the reticulate body(RB). We studied the ultrastructure of Chlamydia trachomatis in the rabbit's conjunctiva using electron microscope (Hitachi H-600). The segments of conjunctiva were cultured in the Eaglis modified essential medium containing 10% fetal calf serum before inoculation of elementary bodies. The results were as follows; 1. The purified elementary body was uniformly spherical particel containing electron-dense material and covered with two lamellated membranes of about 0.3μdiameter. 2. After 12 hrs of infection, elementary bodies were phagocytosed by endocytosis. The elementary body was enclosed by a vacuole made from the cell membrane. 3. After 24 hrs of infection, elementary bodies were changing to reticulate bodies which began to undergo binary fission. 4. After 48 hrs of infection, there were complete changes from elementary bodies to reticulate bodies and numerous glycogen particles in the cytoplasm of cell. 5. After 72 hrs of infection, there were complete of reticulate bodies to elementary bodies and increased number and size of vesicles in cytoplasm of cell.

미분화 소세포폐암에 있어서 Neuron Specific Enolase의 임상적 의의

박성수,김홍근,이정희,김춘원 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

Neuron specific enolase, a distinct isomer of the glycolytic enzyme enolase, is present not only in the central nervous system but also in all neuroendocrine or amine precursor uptake and decarboxylation cells. Neuron specific enolase has also been shown to be present in considerable quantity in small cell lung cancer. To determine the value of serum neuron specific enolase as a tumor marker for diagnosing and monitoring therapeutic response in patients with small cell lung cancer, serum neuron specific enolase was measured by radio-immunoassay in 20 normal controls, 15 patients with non-small cell lung cancer and 14 patients with small cell lung cancer. The mean values were 5.12±2.97ng/㎖ in the control group, 6.60±3.33 ng/㎖ in patients with non-small cell lung cancer, and 34.30ng/㎖ in patients with small cell lung cancer. Patients with small cell lung cancer had a significantly higher mean value than control group and patients with non-small cell lung cancer (P<0.05, P<0.05). Patients with extended stage of small cell lung cancer had significantly higher mean value (45.1±42.97ng/㎖) than patients with limited stage of small cell lung cancer (7.4±1.78ng/㎖). Serial measurements in 6 patients receiving combination chemotherapy showed a correlation in 4 patients between serum neuron specific enolase and therapeutic response. This study indicated that serum neuron specific enolase might be a useful marker for staging, but further investigation is required for monitering therapeutic response in patients with small cell lung cancer.

胃內視鏡 直視下生檢組織에 對한 病理學的 檢索

李貞吉,金春元 한양대학교 의과대학 1983 한양의대 학술지 Vol.3 No.1

One of the the most important indications for gastrofiberscopic examination of the stomach is to prove or disprove gastric mucosa, extension of gastric mucosal pathology including diagnosis of chronic gastritis. Because of the flxibility and easy manuverability of modern fiberendoscopic instrument, there no longer should be any blind areas of stomach for the endoscopist. Thus the combination of gastrofiberscopy with directed biopsy is potentially capable of determining with great accuracy wheather or not malignancy and well tolerated procedure, even in the aged patients and children. Morbidity and mortality are nowadays very low. A histopathologic analysis were performed on 3556 gastrofiberscopic biopsies for understanding gastric diseases among korean. The results were as follows: 1. Distribution of astomach diseases were chronic gastritis 42.7%, gastric adenocarcinoma 23.3%, peptic ulcer 19.4%, and positive diagnosis by gastrofiberscopy was 86.7%. 2. The highest incidence of chronic gastritis was third to fifth decade and the ratio of male to female was 1.7:1. 3. The highest incidence of gastric adenocarcinoma was fourth to sixth decade and the ratio of male to female was 2:1. Histopathologic types of gastric adenocarcinoma were intestinal type 42.9%, undifferentiated type 12.1% and combined type 28.8%, respectively. 4. The highest incidence of peptic ulcer was fourth to fifth decade and the ratio of male to female was 4:1. 5. The highest incidenc of gastric metaplasia was fifth decade and the ratio of male to female was 3:1. 6. Other benign and malignant gastric tumor were polyp 0.9%, squamous cell carcinoma 0.09%, lymphoma 0.06% and leiomyosarcoma 0.03%, respectivly. 7. Concidence ratio between gastrofiberscopic and histopathologic diagnosis were 86.7% of peptic ulcer and 77.1% of gastric adenocarcinoma, respectively.

大腸菌內毒素가 細菌貪食能에 미치는 實驗的 硏究

李寅貴,崔泰悅,金春元 한양대학교 의과대학 1984 한양의대 학술지 Vol.4 No.2

Endotoxemia is frequently experienced clinically, and it rapidly progresses toward death by shock. The endotoxin is a protein-lipopolysacchride complex, which mainly acts upon plasma coagulation factor, platelet, leukocytes and reticuloendothelial system. The liver and spleen are major target organs in endotoxemia. To study the mechanism for the phagocytic and digestive action of reticuloendothelial system, mainly liver and spleen, in rats. An experimental groups were devided as follows; 1 ml normal saline (Group Ⅰ), Staphylococcus aureus 10?CFU 1ml(ATCC 25922, Group Ⅱ), E. coli endotoxin 0.3mg/kg (lipopolysaccharide Difco 026:B6, Group Ⅲ) and Staph. aureus 10?CFU/ml with E. coli endotoxin 0.3mg/kg (Group Ⅳ) were injected into tail vein, respectively. Studies included alkaline phosphatase and alanine aminotransferase in serum, viable colony count of bood, liver and spleen and the histopathogic observation of liver and spleen. The obtained results are follows: 1. Alkaline Phasphatase and alanine aminotransferase are markedly increased in Group Ⅲ and Ⅳ. 2. The number of viable bacteria of blood is gradually decreased according to time in group Ⅱ and Ⅳ, and the number of viable bacteria in group Ⅳ is lesser than Group Ⅱ. 3. The number of viable bacteria of liver and spleen are gradually decreased according to time in group Ⅱ and Ⅳ and the number of viable bacteria in group Ⅳ is lesser than group Ⅱ in liver and spleen. 4. The microscopic finding of liver in group Ⅳ which show severe congestion, infiltration of PMN cells in sinusoid, increased kupffer cells and peripheral type necrosis of hepatic lobule. 5. The microscopic finding of spleen in group Ⅳ which show severe congestion, infiltration of PMN cells in all field and enlarged splenic corpuscle with marginal necrosis.

정신분열증 환자에 있어서 조직적합항원에 관한 연구

구무길,최태열,김춘원 한양대학교 의과대학 1988 한양의대 학술지 Vol.8 No.1

It has been recognized that HLA Class II antigen is more closely related with certain diseases than Class I antigen. In schizophrenic patients, study on HLA antigne done were almost class I antigen. For more clarifying, author studied on the association with Class II antigens in 33 Korean schizphrenic patients under the base of DSM-iii criteria. HLA antigen typeing tray was supplied from UCLA, Terasaki's laboratory. The results are as follows: 1. The relative risk and etiologic fraction of HLA-DR5, and DQw3 are 2.03/0.19 and 1.37/0.16 respectively. 2. In paranoid type, the relative risk and etiologic fraction of HLA-DR5, and DQw3 are 3.12/0.32 and 1.51/0.20 respectively. Since HLA-DQw3 have been known to be associated with DR5 commonly, results from this study provide confirmatory evidence that there is a positive association between HLA-DR5 and schizophrenia especially in paranoid type.