http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
청간해주탕(淸肝解酒湯)이 알코올성 간손상 Proteome에 미치는 영향
정윤종,김영철,우홍정,이장훈,Jung, Yun-Jong,Kim, Young-Chul,Woo, Hong-Jung,Lee, Jang-Hoon 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.1
Objectives : The purpose of our study was to investigate the effects of Chungganhaeju-tang (Qingganjiejiu-tang) on alcoholic liver damage by applying proteomics. Materials and Methods : Sprague-Dawley rats were used in this experiment; the rats were divided into a control group, alcohol group and Chungganhaeju-tang + alcohol group. Ethanol was orally administered twice a day for 4 weeks to the alcohol group. Water without ethanol was administered twice a day for 4 weeks to the control group. Ethanol + Chungganhaeju-tang extract was orally administered twice a day for 4 weeks to the Chungganhaeju-tang + alcohol group. The livers of each group were processed and we investigated histology, OxyBlot, 2-dimensional electrophoresis, and western blot of liver of each group. Results : In the histological findings of the liver, the alcohol group showed portal fibrosis with a few septa or without septa. The Chungganhaeju-tang + alcohol group showed no fibrosis or portalfibrosis without septa. In the OxyBlot finding, Chungganhaeju-tangprevented liver damage by oxidation. In the 2-dimensional electrophoresis finding, formiminotransferase cyclodeaminase (FTCD), glucose regulated protein, 58 kDa (GRP58K), aryl sulfotransferase, sulfotransferase family 1A, member 2, similar to acyl-coenzyme A oxidase-like, and catalase were changed. Conclusion : Chungganhaeju-tangexerts an inhibitory effect against the fibrosis and oxidation induced by alcohol in rat liver cell, and some proteins induced by alcohol were changed by Chungganhaeju-tang.
인진(茵陳)분획물이 간세포활성, 세포주기 및 Fas-mediated Apoptosis에 미치는 영향
김영철,이장훈,우홍정,이종훈,Kim, Young-Chul,Lee, Jang-Hoon,Woo, Hong-Jung,Yi, Jong-Hoon 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.3
Objective : This study was carried out to examine the effect of five fractions of an aqueous extract from Artemisia capillaris $T_{HUNB}$. Methods : The queous extract from Artemisia capillaris $T_{HUNB}$. was fractionized into 5 kinds of material. We observed the effect of each fractions on etoposide-induced apoptosis, cell viability, cell cycle progression and mRNA expression of apoptosis-related genes in human hepatocyte cell line HepG2. Results and Conclusions : The data shows that butanol fraction of Artemisia capillaris $T_{HUNB}$. has no relation with cell cycle, however, it inhibits apoptosis significantly and the action may be due to the suppression of Fas and Sax genes and activation of Bcl-2 gene.
인진청간탕(茵蔯淸肝湯)이 HepG2 cell의 인터페론 신호전달계에 미치는 영향
이종훈,김영철,이장훈,우홍정,Yi, Jong-Hoon,Kim, Young-Chul,Lee, Jang-Hoon,Woo, Hong-Jung 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.1
Objectives/Methods : To analyze the effect of Injinchunggantang(IJCGT) to Interferon-${\alpha}/{\beta}$ signal transmission system in HepG2 cells, HepG2 Cell were treated with IJCGT. Also, revelation of MxA, 2'5'-OAS mRNA leaded by Interferon-${\alpha}/{\beta}$ and revelation and activation of Jak1, TYK1, and STAT 1, all main signal transmission factors, were analyzed. Results : The analysis resulted in the following 1. With interferon ${\alpha}/{\beta}$ there was no affect cell propagation of Hep G2 cells. With IJCGT alone, cell propagation of HepG2 was promoted, and cell propagation control function was recovered. 2. With interferon ${\alpha}/{\beta}$ cell death was unaffected. With IJCGT apoptosis of HepG2 cell was restrained, and the cell's reaction to interferon was unaffected. 3. With interferon ${\alpha}/{\beta}$ treatment mRNA revelation of MxA and 2'5'-OAS was induced. When HepG2 cells were injected with IJCGT without interferon ${\alpha}/{\beta}$ treatment, mRNA revelation of MxA and 2'5'-OAS increased in proportion to the treatment density. With pre-treatment of IJCGT, leaded with interferon ${\alpha}/{\beta}$, promoted revelation of MxA, 2'5' -OAS mRNA. 4. Though mRNA revelation of lakl, TYK1 and STAT1 was unaffected with IJCGT, activation of STAT1 was promoted with an increase of phosphorylation of STAT1 protein. With pre-treatment of IJCGT, Jak1, TYK2, STAT1 phosphorylation, leaded with interferon, strengthened. 5. TNF-a, IL-1b and LPS present, revelation of MxA and 2'5'-OAS mRNA leaded by interferon was restrained when HepG2 cells were treated with IJCGT, and the interferon signal transmission system restraint action leaded by inflammatory cytokines was moderated. Conclusion : These results support a role for IJGCT in promotion of anti-virus action through maintainance of the liver's sensibility toward interferon. A clinical study of an interferon treated patient treated also with IJGCT is needed to determine its efficacy.
만성 B형 간염을 동반한 초기 간경변 환자에서 인진청간탕(茵蔯淸肝湯) 투여 관찰한 5례
이장훈,김세훈,김철우,이지은,김영철,우홍정,정윤종,Lee, Jang-Hoon,Kim, Se-Hoon,Kim, Chul-Woo,Lee, Ji-Eun,Kim, Young-Chul,Woo, Hong-Jung,Jung, Yun-Jong 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.4
Liver cirrhosis is a liverdisease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules. Liver cirrhosis is regarded as a serious health problem with high prevalence in the world as well as in Korea. Liver cirrhosis is an irreversible disease. However, in cases of liver cirrhosis induced by chronic hepatitis B, we should treat the chronic hepatitis B, because it frequently progresses to cirrhosis and hepatoma. We observedimprovements of symptoms and laboratory tests with herb medicine Injinchunggan-tang (茵蔯淸肝湯) in five cases ofliver cirrhosis and chronic hepatitis B. The serum aminotransferase was preserved within normal value levels for a long time, 3 years to 7 years. However, further study is necessary to determine the effect of herb medicine on liver cirrhosis and chronic hepatitis B.
수종의 한약재가 HepG 2.2.15 Cell의 HBeAg발현 억제에 미치는 效果
金榮哲,李長勳,禹弘楨 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1999 東西醫學硏究所 論文集 Vol.1999 No.-
Purpose : Hepatitis B virus DNA transfected cell line(HepG2.2.15) was cultured to evaluate the effect of herbs on the expression of HBeAg and the replication of HBV. HepG2.2.15 produces HBV particles as well as viral proteins into cell culture media. Methods : Extracts of herbs were adminitered to the cells on the proper concentration. Culture media was collected 48 hours after the herbal administration and HBeAg level in the media was examined by ELISA method. To confirm that the anti - viral effect was not due to direct cytotocixity of the extracts, normal cell proliferation was shown by cell counting. And as of the interference in protein synthesis of HepG2.2.15 by herb - extracts, we used the result of study that we performing before by αFP assay using EIA method. Results & Conclusion : Herb medicines like 地楡(Sanguisorbae Radix) and 覆盆子 (Rubi Frusctus) showed significant inhibitory effect on HBeAg expression at P<0.01 and 五味子(Acanthopanacis Cortex) at P<0.05. Whereas, though some herbs such as 茵草根 (Rubiae Radix), 山査(Crataegii Fructus), 白芍藥(Paeoniae Radix Alba), and 大黃(Rhej Radix et Rhizoma) showed the tendecy to suppress HBeAg, most of them were not significant statistically. From the above, we could conclude that those herb medicines can be applied to patients effectively and further studies on effective fraction of some herbs are though to be needed.
Effects of Five Fractions of Artemisia capillaris THUNB on Fas-mediated Apoptosis in HepG2 Cells
Lee, Jang-Hoon,Woo, Hong-Jung,Yi, Jong-Hoon WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1999 東西醫學硏究所 論文集 Vol.1999 No.-
This study was carried out to examine the effect of five fractions of an aqueous extract from Artemisia capillaris THUNB(ACT). on etoposide-induced apoptosis, cell viability, cell cycle progression and mRNA expression of apoptosis-related genes in human hepatocyte cell line HepG2. The data shows that butanol fraction of ACT has no relation with cell cycle, however, it inhibits apoptosis and the action may be due to the suppression of Fas and Bax genes and activation of Bcl-2 gene.
박신명,이지현,이종훈,김영철,이장훈,우홍정 대한한방성인병학회 2000 韓方成人病學會誌 Vol.6 No.1
Primary biliary cirrhosis is a chronic disease of the liver characterized by autoimmune destruction of intrahepatic bile ducts and cholestasis. Primary biliary cirrhosis will cause cholestasis that may progress to cirrhosis and finally result in the liver failure. The pathogenesis of primary biliary cirrhosis is uncertain, however there is a close association between primary biliary cirrhosis and antimitochondrial antidody. Primary biliary cirrhosis is a relatively common autoimmune liver disease in western conuntry. But it is rare in Asia. Primary biliary cirrhosis affects mainly middle female. There is no definitive drug treatment, liver transplantation is indicated for patients with intractable symptoms and end-stage disease. The authors have experienced a case of primary biliary cirrhosis in 48 year-old female who had suffered from jaundice, ascites, fatigue and indigestion. We report a case of primary biliary cirrhosis with a review of literatures.