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수종의 한약재가 HepG 2.2.15 Cell의 HBeAg발현 억제에 미치는 효과(效果)
우홍정,이장훈,김영철,Woo, Hong-Jung,Lee, Jang-Hoon,Kim, Young-Chul 대한한방내과학회 1999 大韓韓方內科學會誌 Vol.20 No.1
Purpose : Hepatitis B virus DNA transfected cell line(HepG2.2.15) was cultured to evaluate the effect of herbs on the expression of HBeAg and the replication of HBV. HepG2.2.15 produces HBV particles as well as viral proteins into cell culture media. Methods : Extracts of herbs were adminitered to the cells on the proper concentration. Culture media was collected 48 hours after the herbal administration and HBeAg level in the media was examined by ELISA method. To confirm that the anti-viral effect was not due to direct cytotocixity of the extracts, normal cell proliferation was shown by cell counting. And as of the interference in protein synthesis of HepG2.2.15 by herb-extracts, we used the result of study that we performed before by ${\alpha}FP$ assay using EIA method. Results& Conclusion : Herb medicines like 地楡(Sanguisorbae Radix) and 覆盆子(Rubi Frusctus) showed significant inhibitory effect on HBeAg expression at p<0.01 and 五味子(Acanthopanacis Cortex) at p<0.05. Whereas, though some herbs such as ?草根(Rubiae Radix), 山査(Crataegii Fructus), 白芍藥(Paeoniae Radix Alba), and 大黃(Rhei Radix et Rhizoma) showed the tendecy to suppress HBeAg. most of them were not significant statistically. From the above, we could conclude that those herb medicines can be applied to patients effectively and further studies on effective fraction of some herbs are thought to be needed.
茵蔯淸肝蕩이 HepG 2.2.15 Cell의 HBeAg발현 억제에 미치는 效果
金善珉,李長勳,禹弘楨 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1998 東西醫學硏究所 論文集 Vol.1998 No.-
Background and Purpose Hepatitis B virus DNA transfected cell line(HepG2.2.15) was cultured to evaluate the effect of oriental medicine on the expression of HBeAg and the replication of HBV. HepG2.2.15 produces HBV particles as well as viral preteins into cell culture media. Methods & Results Injinchunggantang-extract was administered on the cells on the proper concentration determined by the preliminary test. Culture media was collected 48 hours after the herbal administration and HBeAg level in the media was examined by ELISA method. To confirm that the anti-viral effect was not due to cytotocixity of Injinchunggantang-extract normal cell proliferation was shown by cell counting. And no interference in protein synthesis of HepG2.2.15 by Injinchunggantang-extract was confirmed by αFP assay using EIA method.
박용진,金榮哲,李長勳,禹弘楨 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1998 東西醫學硏究所 論文集 Vol.1998 No.-
The purpose of this study is to evaluate the effect of Injinchunggantang-derivative on proliferation of hepatocyte in rats. Cell viability is studied by MTT assay. The gene related to cell replication such as p53, waf1, bcl-2 and bcl-x?? is quantitized by quantitative RT-PCR and the proteins coded by these genes are studied by Western blotting. The results are as follows. 1. The hepatocytes cultured in medium with Injinchunggantang-derivative showed better viability spared with control group in MTT assay, and the hepatocytes cultured in medium with the Injinchunggantang-derivative-and-ethanol-mixed group showed better viability than the hepatocytes cultrued in 10% ethanol culture medium(control group), noting that Injinchunggantang-derivative has protective effect on hepatocyte injury. There was no dose- and time-dependence. 2. In quantitative RT-PCR, ⅰ)Bcl-2 gene increased significantly both in Injinchunggantang-derivative group and in Inchunggantang-derivative-and-ethanol-mixed group, while it showed no significant increase or decrease in other group. ⅱ) Bcl-xl gene increased significantly in Injinchunggantang-derivative group as well as in Injinchunggantang-derivative-and-ethanol-mixed group. ⅲ) P53 gene showed no significant increase or decrease in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchunggantang-derivative-and-ethanol-mixed group, suggesting that 10% ethanol induced cell toxicity, thus increased gene expression. ⅳ) Waf1 gene showed no significant increase or decrease in hepatocytes cutured in medium with Injinchunggantang-derivative, while increased in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchunggantang-derivative-and-ethanol-mixed group, suggesting that 10% ethanol induced cell toxicity increased waf1 gene expression. 3. In the study on protein by western blotting, the band of bcl-2 and bcl-xl were widened in Injinchunggantang-derivative group. Especially the amount of bcl-xl increased significantly compared with other groups. But in the study on p53 and waf1, there was no significant difference among those groups. Above study shows that Injinchunggantang-derivative has good effect on cell viability and that the genes resistant to cell death such as bcl-2 and bcl-xl are induced Injinchunggantang-derivative to resist to cell death by toxic agent. And this is reconfirmed protein study using western blotting. These results suggest that Injinchunggantang-derivative has inhibitory effect on cell death as well as protective effect on hepatocyte. Therefore this prescription is recommended in various liver disease such as chronic liver disease drug-induced hepatic injury.
기혈음양허손(氣血陰陽虛損) 변증(辨證) 분석을 위한 설문문항 개발
우홍정,김세훈,이승보,최미영,김영철,이장훈,Woo, Hong-Jung,Kim, Se-Hoon,Lee, Seung-Bo,Choi, Mi-Young,Kim, Young-Chul,Lee, Jang-Hoon 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.4
Objectives : Consumption is a chronic wasting disease and major portion of Oriental Medicine's therapy. However, there is no standard diagnostic method for consumption that is $q{\grave{i}}-x{\bar{u}}$, $xu{\grave{e}}-x{\bar{u}}$, $yang-x{\bar{u}}$, $y{\bar{i}}n-x{\bar{u}}$. Methods : A questionnaire which includes symptoms and signs for diagnosis of $q{\grave{i}}-x{\bar{u}}$, $xu{\grave{e}}-x{\bar{u}}$, $yang-x{\bar{u}}$, $y{\bar{i}}n-x{\bar{u}}$ was evaluated by Delphi technique. Each question was valuated by interviewing 27 oriental medicine doctors. Then. we choose questions given over 5 points and reorganized some items according to the recommendations by interviewed-doctors. We then accessed the value of re-organized questions composing of the questionnaires. Conclusion : We finally chose each 9 items of $q{\grave{i}}-x{\bar{u}}$, $xu{\grave{e}}-x{\bar{u}}$, $yang-x{\bar{u}}$, $y{\bar{i}}n-x{\bar{u}}$'s questionnaire. Further study is necessary for modification of questionnaire by statistics and certification by clinical trial.