Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

Yang Yang,Cheng Zhimin,Zhang Wanfeng,Hei Wei,Lu Chang,Cai Chunbo,Zhao Yan,Gao Pengfei,Guo Xiaohong,Cao Guoqing,Li Bugao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p< 0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism. Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

On a perturbed Sparre Andersen risk model with dividend barrier and dependence

Zhimin Zhang,Xiu Wu,Hu Yang 한국통계학회 2014 Journal of the Korean Statistical Society Vol.43 No.4

In this paper, we consider a Sparre Andersen risk model perturbed by a Brownian motion,where the inter-claim time and individual claim size follow some bivariate distribution. Assume that a barrier dividend strategy is applied to the surplus process, so that dividendsare paid out whenever the surplus level attains a barrier b. Integral equations and integrodifferentialequations satisfied by the Gerber–Shiu discounted penalty functions and theexpected discounted dividend payments are derived, and solutions are also given for somespecial cases.

Asymmetric reduction of chloroacetophenones to produce chiral alcohols with microorganisms

Zhimin Ou,Jianping Wu,Lirong Yang,Peilin Cen 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.1

Four strains of yeast with reduction activity of chloroacetophenones were screened, in which Saccharomyces cerevisiae B5 showed best reduction activity and stereoselectivity. High optical purity (R)-2'-chloro-1-phenylethanol can be obtained with Saccharomyces cerevisiae B5 as biocatalyst. The influence of several co-substrates on the enantiometric excess (ee%) and yield of (R)-2'-chloro-1-phenylethanol was evaluated. 5% (v/v) ethanol is optimal cosubstrate for (R)-2'-chloro-1-phenylethanol formation. The optimal bioconversion conditions of 2'-chloroacetophenone catalyzed by Saccharomyces cerevisiae B5 are as follows: pH 8.0, 25 oC and 24 h. The yield and the enantiometric excess of (R)-2'-chloro-1-phenylethanol can both reach more than 99% with 10.75 g/l Saccharomyces cerevisiae B5 (the cell dry weight) and 1 g/l 2'-chloroacetophenone used in the biotransformation.

Asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester by undifferentiated cells of white turnip in phosphate buffer/organic solvent

Zhimin Ou,Qingmei Chen,Gensheng Yang,Li Xu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.2

Ethyl (S)-3-hydroxy-3-phenylpropionate was synthesized by asymmetric reduction of 3-oxo-3-phenylpropionic acid ethyl ester with undifferentiated cells of white turnip in phosphate buffer/organic solvent. The conversion increased with the LogP_oct of organic solvent increase. The phosphate buffer (0.2 mol/L, pH 7.0)/dodecane was selected as optimum medium for reduction. The optimal content of dodecane in medium is 10% (v). The conversion decreased with initial substrate concentration increase. Addition of more biomass of plant cells and 10% ethanol as co-substrate can improve conversion. The plant cells can be reused well for three times. The enantiomeric excess of ethyl (S)-3-hydroxy-3-phenylpropionate reached 100% with 1% allyl bromide as inhibitor.

When does surplus reach a given target before ruin in the Markov-modulated diffusion model?

Hu Yang,Zhimin Zhang 한국통계학회 2010 Journal of the Korean Statistical Society Vol.39 No.2

In this paper, we consider a Markov-modulated diffusion risk model. We study when the surplus reaches a given level b .U.0// before ruin. We show that the Laplace transform of such random time can be expressed via the expected discounted penalty functions. Finally, we modify the Markov-modulated diffusion model by a barrier dividend strategy,and give some expressions for the expected discounted penalty functions and the expected discounted dividend payments.

Removal of Cr(VI) from aqueous solution by rice husk derived magnetic sorbents

Ruifeng Yang,Shangru Zhai,Yuan Fan,Zhimin Lei,Na Liu,Jialiang Lv,Bin Zhai,Lei Wang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.4

−A novel magnetic porous sorbent obtained from agricultural waste rice husk was successfully synthesized through a simple carbon-thermal method. The sorbent was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometer, N2 sorption analysis, and X-ray photoelectron spectroscopy. The removal efficiency of the sorbent for Cr(VI) was also investigated. Chromium adsorption was fitted by the pseudo-second-order and Langmuir models. The maximum chromium adsorption capacity, Brunauer-Emmett-Teller surface area, and average Barrett-Joyner-Halenda pore size of the magnetic sorbent were 157.7mg·g−1, 134.1m2·g−1, and 4.99 nm, respectively. The saturated magnetization of the novel adsorbent was 77.8 emu·g−1, indicating that the material can facilitate separation and recovery from aqueous systems. The removal mechanisms of Cr(VI) were also discussed. The result illustrates that rice husk-derived magnetic carbonaceous materials are a potential adsorbent for Cr(VI) pollution treatment and provide a suitable method for the effective conversion of biomass waste, which may solve the problem of waste disposal and widen the applications of the materials.

MoO3 modified CeO2/TiO2 catalyst prepared by a single step sol–gel method for selective catalytic reduction of NO with NH3

Ye Jiang,Zhimin Xing,Xuechong Wang,Shanbo Huang,Qingyu Liu,Jingshan Yang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.29 No.-

A MoO3 modified CeO2/TiO2 catalyst was prepared by a single step sol–gel method for selective catalyticreduction of NO with NH3. The experimental results indicates that MoO3 modified CeO2/TiO2 catalystexhibited higher SCR activity and resistance to 10% H2O and 1000 ppm SO2. From the characterizationresults, it can be concluded that good physical properties, highly dispersed active species of Ce and Mo,more appearance of Ce3+ and chemisorbed oxygen, strong interaction among ceria, molybdenum andtitania, and high oxygen storage capacity might contribute to the excellent deNOx performance of CeO2–MoO3/TiO2 catalyst prepared by a single step sol–gel method.

On Privacy and Anonymity in Freenet System

Tianbo Lu,Zhimin Lin,Lingling Zhao,Yang Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5

As a typical representative of anonymous network applications, Freenet system has so many advantages in sharing, privacy, anonymity as well as uploading and downloading convenience that it adequately realizes expression freedom. So it’s widely used in different fields. For Freenet system, academia and system designers have been conducting research and improvement which mainly on following points: structure, topology, routing algorithm and the Darknet mechanism which proposed by new version. The purpose of these efforts is to increase the system network utilization, enhance the reliability of transmission and improve the safety and robustness of the system. This paper reviews and summarizes the research progress of Freenet system by collating and analyzing relevant articles. We also analyze and compare the main ideas, the algorithm application as well as pros and cons of different articles surrounding different topics. In addition, we also tease out the development and the evolution trends of Freenet system in time order, and combined with current network situation, we made reasonable proposals and prospects and draw scientific conclusions.

Correction to: Effects of solvent extraction on the microstructure of bituminous coal‑based graphite

Lipeng Wang,Zongxu Yao,Zhimin Guo,Xiaofeng Shen,Zhiang Li,Zhengqi Zhou,Yuling Wang,Jian‑Guo Yang 한국탄소학회 2022 Carbon Letters Vol.32 No.3

Stress-Release Law and Deformation Characteristics of Large-Span Tunnel Excavated with Semi Central Diaphragm Method

Shen Zhou,Liping Li,Zhimin An,Hongliang Liu,Guangyu Yang,Pengfei Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.6

The proportion of large-span tunnels in newly-built highway tunnels is getting higher and higher, revealing the stress release law and deformation characteristics of the tunnel at different excavation steps has important guiding significance for the rational design of the supporting structure parameters. In this paper, a typical section of the Laohushan Tunnel was selected to analyze the stress and deformation response of the large-span tunnel excavated with the semi central diaphragm method (SCDM), with the numerical simulation and field monitoring method. The study found that during the excavation of the large-span tunnel with the SCDM, the stress and deformation response characteristics are different from those ordinary tunnels. Influenced by the settlement of the vault, there was a significant outward expansion tendency, and a high degree of stress concentration in the sidewall. In particular, the supporting structure at the sidewall has a deformation characteristic of expanding outward and then converging inward. In this paper, the in-depth analysis of the stress release law and deformation characteristics of the excavation process of large-span tunnels can provide reference for the excavation and support parameter design of similar projects.