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전윤수,허 정 대구효성가톨릭대학교 1994 연구논문집 Vol.49 No.1
The purpose of this study was to investigate the effect of mental practice on shooting performance. The subjects were 20 male students who were belonging to the department of Physical Education in I city. They were randomly devided into two groups (control and experimental group). The subjects in experimental group were trained by Skinks rifle video tape for 8 weeks and the subjects in control group were not trained for 8 weeks. Before and after 8 weeks training, All subjects performed 10 M rifle shooting and the records were analyzed by t-test. The results were summarized as follows; 1. After 8 weeks training, the performance of control group in shooting was increased but it was not shown a significant level. 2. After 8 weeks training, the performance of experimental group in shooting was significantly increased.(p< .001) 3. After 8 weeks training, the performance of experimental group in shooting was significantly higher than that of control group. (p< .01)
Heo, Yun-Jeong,Chung, In-Young,Cho, Wan-Je,Lee, Bo-Young,Kim, Jung-Hoon,Choi, Kyoung-Hee,Lee, Jin-Won,Hassett, Daniel J.,Cho, You-Hee American Society for Microbiology 2010 Journal of Bacteriology Vol.192 No.2
<B>ABSTRACT</B><P>The adaptive response to hydrogen peroxide (H2O2) in <I>Pseudomonas aeruginosa</I> involves the major catalase, KatA, and OxyR. However, neither the molecular basis nor the relationship between the aforementioned proteins has been established. Here, we demonstrate that the transcriptional activation of the <I>katA</I> promoter (<I>katAp</I>) in response to H2O2 was abrogated in the <I>P. aeruginosa</I> PA14 <I>oxyR</I> null mutant. Promoter deletion analyses revealed that H2O2-mediated induction was dependent on a region of DNA −76 to −36 upstream of the H2O2-responsive transcriptional start site. This region harbored the potential operator sites (OxyR-responsive element [ORE]) of the <I>Escherichia coli</I> OxyR binding consensus. Deletion of the entire ORE not only abolished H2O2-mediated induction but also elevated the basal transcription, suggesting the involvement of OxyR and the ORE in both transcriptional activation and repression. OxyR bound to the ORE both <I>in vivo</I> and <I>in vitro</I>, demonstrating that OxyR directly regulates the <I>katAp</I>. Three distinct mobility species of oxidized OxyR were observed in response to 1 mM H2O2, as assessed by free thiol trapping using 4-acetamido-4′-maleimidylstilbene-2,2′-disulfonic acid. These oxidized species were not observed for the double mutants with mutations in the conserved cysteine (Cys) residues (C199 and C208). The uninduced transcription of <I>katAp</I> was elevated in an <I>oxyR</I> mutant with a mutation of Cys to serine at 199 (C199S) and even higher in the <I>oxyR</I> mutant with a mutation of Cys to alanine at 199 (C199A) but not in <I>oxyR</I> mutants with mutations in C208 (C208S and C208A). In both the C199S and the C208S mutant, however, <I>katAp</I> transcription was still induced by H2O2 treatment, unlike in the <I>oxyR</I> null mutant and the C199A mutant. The double mutants with mutations in both Cys residues (C199S C208S and C199A C208S) did not differ from the C199A mutant. Taken together, our results suggest that <I>P. aeruginosa</I> OxyR is a bona fide transcriptional regulator of the <I>katA</I> gene, sensing H2O2 based on the conserved Cys residues, involving more than one oxidation as well as activation state <I>in vivo</I>.</P>
Heo Jinbeom,Lee Jinyoung,Nam Yun Ji,Kim YongHwan,Yun HongDuck,Lee Seungun,Ju Hyein,Ryu Chae-Min,Jeong Seon Min,Lee Jinwon,Lim Jisun,Cho Yong Mee,Jeong Eui Man,Hong Bumsik,Son Jaekyoung,Shin Dong-Myung 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Aberrant activation of embryogenesis-related molecular programs in urothelial bladder cancer (BC) is associated with stemness features related to oncogenic dedifferentiation and tumor metastasis. Recently, we reported that overexpression of transcription factor CP2-like protein-1 (TFCP2L1) and its phosphorylation at Thr177 by cyclin-dependent kinase-1 (CDK1) play key roles in regulating bladder carcinogenesis. However, the clinical relevance and therapeutic potential of this novel CDK1-TFCP2L1 molecular network remain elusive. Here, we demonstrated that inhibitor of DNA binding-2 (ID2) functions as a crucial mediator by acting as a direct repressive target of TFCP2L1 to modulate the stemness features and survival of BC cells. Low ID2 and high CDK1 expression were significantly associated with unfavorable clinical characteristics. TFCP2L1 downregulated ID2 by directly binding to its promoter region. Consistent with these findings, ectopic expression of ID2 or treatment with apigenin, a chemical activator of ID2, triggered apoptosis and impaired the proliferation, suppressed the stemness features, and reduced the invasive capacity of BC cells. Combination treatment with the specific CDK1 inhibitor RO-3306 and apigenin significantly suppressed tumor growth in an orthotopic BC xenograft animal model. This study demonstrates the biological role and clinical utility of ID2 as a direct target of the CDK1-TFCP2L1 pathway for modulating the stemness features of BC cells.
Heo, Yun-Jeong,Lee, Yu-Rim,Jung, Hyun-Hee,Lee, JungEun,Ko, GwangPyo,Cho, You-Hee American Society for Microbiology 2009 Antimicrobial agents and chemotherapy Vol.53 No.6
<B>ABSTRACT</B><P>Phage therapy against <I>Pseudomonas aeruginosa</I> infections has received renewed attention owing to the increasing prevalence of antibiotic resistance in this bacterium. Here, we isolated and characterized two new potentially lytic bacteriophages (MPK1 and MPK6), which produced large and clear plaques on <I>P. aeruginosa</I> strain PAO1. Based on their morphology, MPK1 belongs to the <I>Myoviridae</I>, while MPK6 belongs to the <I>Podoviridae</I>. The group B polysaccharide of lipopolysaccharide was required for infection, suggesting that their host spectra are associated with the serotypes of <I>P. aeruginosa</I> strains. Intramuscular and intraperitoneal administration of MPK1 and, to a lesser extent, MPK6 significantly protected mice from mortality caused by PAO1-induced peritonitis-sepsis (<I>P</I> < 0.01). Mice treated with either phage also had lower bacterial burdens in their livers, lungs, and spleens. The antibacterial efficacy of MPK1 and MPK6 was also evaluated based on <I>Drosophila melanogaster</I> systemic infection caused by <I>P. aeruginosa</I>, for which phages were administered by feeding. Both phages significantly delayed the PAO1-induced killing of <I>D. melanogaster</I> (<I>P</I> < 0.001), although MPK1 persisted longer than MPK6 in uninfected <I>D. melanogaster</I> tissue samples. These results suggest that a mini-scale experiment using <I>D. melanogaster</I> infection is valid for evaluating the antibacterial efficacy of phage therapy against <I>P. aeruginosa</I> infections.</P>
Heo, Yun-Jeong,Jeon, Moon-Kook Elsevier 2017 Tetrahedron Vol.73 No.40
<P><B>Abstract</B></P> <P>This work describes a solid-phase synthetic method for building a compound library of <I>N</I>-alkyl-4-alkylamino-1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine-6-carboxamide derivatives, that are based on the biologically active 1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine scaffold. In the first step of this synthetic sequence, condensation reactions of ethyl 5-amino-1-aryl-1<I>H</I>-pyrazole-4-carboxylates with methyl cyanoformate resulted in the formation of esters that underwent hydrolysis to give 1-aryl-4,5-dihydro-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidin-4-one-6-carboxylic acids. The coupling reaction of these carboxylic acids with primary alkylamine-loaded acid-sensitive methoxybenzaldehyde (AMEBA) resins was followed by amination reactions mediated by benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP). Subsequent cleavage from the solid support resulted in the formation of the target <I>N</I>-alkyl-4-alkylamino-1-aryl-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidine-6-carboxamide derivatives. The reaction conditions for solid-phase transformations were optimized using a solution-phase model study with 2,4-dimethoxybenzyl-protected isobutylamine as a reactant in place of the AMEBA resin-loaded isobutylamine. The progress of the solid-phase reactions was monitored by on-bead ATR-FTIR spectroscopy. Diversification experiments were performed by using 1-aryl-4,5-dihydro-1<I>H</I>-pyrazolo[3,4-<I>d</I>]pyrimidin-4-one-6-carboxylic acids and a variety of primary and secondary amine building blocks to build the target compound library.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Heo, Yun-Jeong,Chung, In-Young,Choi, Kelly B.,Lau, Gee W.,Cho, You-Hee Microbiology Society 2007 Microbiology Vol.153 No.9
<P>A temperate transposable bacteriophage (MP22) was isolated from a Korean clinical isolate of Pseudomonas aeruginosa. It has a coliphage lambda-like morphology and a double-stranded DNA genome. The complete nucleotide sequence and annotation of the MP22 genome and its characteristics are presented. The MP22 genome is 36 409 bp long with a G+C content of 64.2 mol%. The genome contains 51 proposed ORFs, of which 48 (94 %) display synteny and significant nucleotide and protein sequence similarity to the corresponding ORFs of the closely related phage, D3112. Three of the predicted ORFs are unique proteins, whose functions are yet to be revealed. The phage c repressors exhibit striking dissimilarities and, when present as a single gene, did not show cross-immunity. In contrast, although an MP22 lysogen could be productively infected with D3112, MP22 could not grow on a D3112 lysogen, indicating a role of other D3112 genes in superinfection exclusion.</P>