Effects of ergothioneine-enriched mushroom extract on oxidative stability, volatile compounds and sensory quality of emulsified sausage

Tao, Ye,Xiao, Shan,Cai, Jiaming,Wang, Jihui,Li, Lin Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10

Objective: The aim of this work was to assess the effect of ergothioneine (ESH)-enriched mushroom extract on oxidative stability, volatile compounds, and sensory quality of emulsified sausage. Methods: The ESH content was determined by high performance liquid chromatography. The antioxidant activity of Flammulina velutipes (F. velutipes) extract was determined through radical-scavenging activity of 1,1 diphenyl-2-picryl-hydrazyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radicals. Four different groups of emulsified sausage were manufactured: control, no antioxidants; BHA, 0.01% butylated hydroxyanisole; EEME, 0.8% ESH-enriched mushroom (F. velutipes) extract; AE, 0.012% authentic ESH, after storage for 14 days (at 4℃), the quality of sausage including oxidative stability (2-thiobarbituric acid reactive substances and protein carbonyls content), volatile compounds and sensory quality were studied. Results: It was demonstrated that adding ESH-enriched F. velutipes extract to sausage could effectively prevent lipid and protein oxidation, and its efficacy was equivalent with 0.01% BHA. During meat processing, the ESH mainly contributed to the antioxidative activity of F. velutipes extract. The flavor and sensory attributes of emulsified sausage were improved through adding ESH-enriched F. velutipes extract. Conclusion: Accordingly, the extract of F. velutipes contained high-level of ESH and could be a good antioxidant candidate for processed meat production.

LncRNA-IMAT1 Promotes Invasion of Meningiomas by Suppressing KLF4/hsa-miR22-3p/Snai1 Pathway

Tao Zhang,Yu Ge,Daijun Wang,Qin Liu,Shuchen Sun,Lingyang Hua,Jiaojiao Deng,Shihai Luan,Haixia Cheng,Qing Xie,Ye Gong,Tao Zhang 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.6

Malignant meningiomas often show invasive growth that makes complete tumor resection challenging, and they are more prone to recur after radical resection. Invasive meningioma associated transcript 1 (IMAT1) is a long noncoding RNA located on Homo sapiens chromosome 17 that was identified by our team based on absolute expression differences in invasive and non-invasive meningiomas. Our studies indicated that IMAT1 was highly expressed in invasive meningiomas compared with non-invasive meningiomas. In vitro studies showed that IMAT1 promoted meningioma cell invasion through the inactivation of the Krüppel-like factor 4 (KLF4)/hsa-miR22-3p/Snai1 pathway by acting as a sponge for hsa-miR22-3p, and IMAT1 knockdown effectively restored the tumor suppressive properties of KLF4 by preserving its tumor suppressor pathway. In vivo experiments confirmed that IMAT1 silencing could significantly inhibit the growth of subcutaneous tumors and prolong the survival period of tumor-bearing mice. Our findings demonstrated that the high expression of IMAT1 is the inherent reason for the loss of the tumor suppressive properties of KLF4 during meningioma progression. Therefore, we believe that IMAT1 may be a potential biological marker and treatment target for meningiomas.

Bavachin Suppresses Alpha-Hemolysin Expression and Protects Mice from Pneumonia Infection by Staphylococcus aureus

( Ye Tao ),( Dazhong Sun ),( Xinran Ren ),( Yicheng Zhao ),( Hengjian Zhang ),( Tao Jiang ),( Jiyu Guan ),( Yong Tang ),( Wu Song ),( Shuqiang Li ),( Li Wang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Staphylococcus aureus (S. aureus) infection causes dramatic harm to human health as well as to livestock development. As an important virulence factor, alpha-hemolysin (hla) is critical in the process of S. aureus infection. In this report, we found that bavachin, a natural flavonoid, not only efficiently inhibited the hemolytic activity of hla, but was also capable of inhibiting it on transcriptional and translational levels. Moreover, further data revealed that bavachin had no neutralizing activity on hla, which did not affect the formation of hla heptamers and exhibited no effects on the hla thermal stability. In vitro assays showed that bavachin was able to reduce the S. aureus-induced damage of A549 cells. Thus, bavachin repressed the lethality of pneumonia infection, lung bacterial load and lung tissue inflammation in mice, providing potent protection to mice models in vivo. Our results indicated that bavachin has the potential for development as a candidate hla inhibitor against S. aureus.

Markov switching quantile regression models with time-varying transition probabilities

Tao Ye,Yin Juliang 한국통계학회 2022 Journal of the Korean Statistical Society Vol.51 No.3

Markov switching models are widely used in the time series field for their ability to describe the impact of latent regimes on the behaviour of response variables. Meanwhile, Markov switching quantile regression models with fixed transition probabilities (MSQR-FTP) also provide rich dynamics to modeling financial data, however, it is not always clear how to describe the dynamics on the transition probabilities. This paper extends the transition probabilities to be the time-varying case by allowing them to include information from related variables. By establishing a connection between a quantile regression and an asymmetric Laplace distribution, this paper proposes a maximum likelihood estimation (MLE) method for MSQR-TVTP, and shows the consistency of the MLE. Finally, the performance of the proposed method is illustrated through a simulation study. As an empirical application, we further apply the method to the S&P 500 weekly percentage returns.

High‑speed growth of high‑quality polycrystalline diamond films by MPCVD

Kai Chen,Tao Tao,Wenxiao Hu,Yucong Ye,Kaiwen Zheng,Jiandong Ye,Ting Zhi,Xiwei Wang,Bin Liu,Rong Zhang 한국탄소학회 2023 Carbon Letters Vol.33 No.7

High-quality diamond films have attracted extensive attentions due to their excellent optical and electrical properties. However, several issues, such as random orientation, stress accumulation, and slow growth rate, severely limit its applications. In this paper, high-quality polycrystalline diamond films with highly ordered (100) orientation were prepared by microwave plasma chemical vapor deposition. The effects of growth parameters on the microstructure, quality and residual stress of diamond films were investigated. Experimental results indicate that relatively high temperature at low methane concentration will promote the formation of (100) oriented grains with a low compressive stress. Optimized growth parameters, a methane concentration of 2% along with a pressure of 250 Torr and temperature at 1050 ℃, were used to acquire high growth rate of 7.9 μm/h and narrow full width at half maximum of Raman peak of 5.5 cm? 1 revealing a high crystal quality. It demonstrates a promising method for rapid growth of high-quality polycrystalline diamond films with (100) orientation, which is vital for improving the diamond related applications at low cost.

Secure DNP3 Services Scheme in Smart Grid Link Layer Based on GCM-AES

Ye Lu,Tao Feng,Guohua Ma 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.8

This paper defines a new DNP3 link layer frame structure based on the link layer packet characteristics of transmission time requirements and security requirements in substation automation system (SAS). The new frame structure can provide three different work modes: authentication, authorization-encryption, non-authenticated encryption. Then we propose a link-layer security service mechanism in substation automation system based on the GCM-AES, including the session key agreement protocol based on EKE, GMAC-based message authentication protocol, GCM-AES Authentication Encryption-based DNP3 protocol and GCM-AES-based message transform algorithm. Through experimental calculation and analysis, the results show that the new security mechanisms achieve the efficient and safety in substation packets transmission.

PXR Mediated Protection against Liver Inflammation by Ginkgolide A in Tetrachloromethane Treated Mice

Ye, Nanhui,Wang, Hang,Hong, Jing,Zhang, Tao,Lin, Chaotong,Meng, Chun The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.1

The pregnane X receptor (PXR), a liver and intestine specific receptor,, has been reported to be related with the repression of inflammation as well as activation of cytochromosome P450 3A (CYP3A) expression. We examined the effect of PXR on tetrachloromethane (CCl4)-induced mouse liver inflammation in this work. Ginkgolide A, one main component of Ginkgo biloba extracts (GBE), activated PXR and enhanced PXR expression level, displayed both significant therapeutic effect and preventive effect against $CCl_4$-induced mouse hepatitis. siRNA-mediated decrease of PXR expression significantly reduced the efficacy of Ginkgolide A in treating $CCl_4$-induced inflammation in mice. Flavonoids, another important components of GBE, were shown anti-inflammatory effect in a different way from Ginkgolide A which might be independent on PXR because flavonoids significantly inhibited CYP3A11 activities in mice. The results indicated that anti-inflammatory effect of PXR might be mediated by enhancing transcription level of $I{\kappa}B{\alpha}$ through binding of $I{\kappa}B{\alpha}$. Inhibition of NF-${\kappa}B$ activity by NF-${\kappa}B$-specific suppressor $I{\kappa}B{\alpha}$ is one of the potential mechanisms of Ginkgolide A against CCl4-induced liver inflammation.

Chemical reconstruction of Castor Oil Research of Environmentally Friendly Lubricants

TAO De-hua,YE bin 한국트라이볼로지학회 2002 한국트라이볼로지학회 학술대회 Vol.2002 No.10

Natural castor oil was chemically reconstructed to extend the carbon chains by means of iso-reaction so as to improve the rheological behavior, by way of increasing the viscosity index and decreasing the pour point. The rheoloaical and triboloaical characteristics of the reconstructed castor oil were comparatively investigated with those of the natural castor oil and several other vegetable oils and a mineral oil. The friction and wear test results on a four-ball machine indicate that the chemically reconstructed castor oil has considerably improved rheological and tribological properties as compared with the natural castor oil. It shows a greatly increase viscosity index and largely decreased pour point, which makes it applicable to low temperature lubrication. The chemically reconstructed castor oil even shows better tribological behavior than pentaerythritol ester or di-iso-capryl sebacate. However, it is still needed to increase the oxidation stability of the reconstructed castor oil.

Probability Based Virtual Machines Placement for Green Data Center

Ye Heng-zhou,Li Tao-shen 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.12

Virtual Machine Placement (VMP) is regarded as an important criterion to improve resource utilization and reduce energy consumption for cloud data centers. The existing VMP schemes simply set the VM resource requirements fixed values and ignore their fluctuation characteristics. Assuming normal distribution resource requirements, we firstly present a model for data centers based on a more accurate energy consumption model for single machine. Then, an effective genetic algorithm is adopted to solve this model. In the algorithm, some important issues, such as the number of population, fitness function and calculating method of energy consumption are discussed. In the end, we validate our method by experiments.

Innovation Mechanism Study Based On The Technological Convergence : Implications For IT Enterprises

Ye Xu,Changqi Tao 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.9

Based on redefining the concept of technological convergence, this paper analyzes market demand of convergent products according to the characteristics of technological convergence in IT industry. We define market power as the function of product price and the improvement of convergent product’s performance. We also assume the original technology is symbiotic. Based on the above analysis, the paper modifies the Bass Model and the hypotheses of related models, builds a demand growth model of technological convergent products, and uses this model to analyze and estimate the demand growing of converged products. At last, we discuss the conditions that technological convergence will be successful.