Gene Expression Patterns of Cutaneous Squamous Cell Carcinoma and Actinic Keratosis: Biomarkers Screening for Skin Disease Diagnosis

( Xiaojing Li ),( Zhifeng Li ),( Zhao Han ),( Ling Zhang ),( Zhao Liu ),( Baoguo Liu ) 대한피부과학회 2018 Annals of Dermatology Vol.30 No.5

Background: Actinic keratosis (AK) was an intraepidermal tumor which caused by ultraviolet irradiation-induced skin damage. Objective: The aim was to screen biomarkers for development of skin disease by comparing the gene expression profiles between cutaneous squamous cell carcinoma (CSCC) and AK. Methods: GSE45216 with 30 cutaneous squamous cell carcinoma patients and 10 actinic keratosis patients were downloaded and significance analysis of microarrays was processed to screen differently expressed genes (DEGs). Fisher’s exact test was processed for DEGs enrichment. Pathway relationship network systematically reflected the signal conduction and synergism between enriched pathways based on Kyoto Encyclopedia of Genes and Genomes database. Gene co-expression network was constructed according to gene expression data. Quantitative real- time-PCR was used to verify screened biomarkers. Results: Total 410 DEGs were screened and enriched into various functions, such as signal transduction and negative regu-lation of apoptotic process. They also participated into cytokine- cytokine receptor interaction and focal adhesion. The pathway relationship network was constructed with 27 nodes. Hub nodes with higher degree of this network were mitogen-activated protein kinase signaling pathway and apoptosis. The gene co-expression network was constructed with 39 nodes. Thereinto, hub node was ELOVL fatty acid elongase. The expression levels of ELOVL4 and HPGD were significantly higher in CSCC samples than that in AK samples, while the expression levels of INHBA and LAMC2 in CSCC samples were significantly lower than that in AK samples. Conclusion: These screened genes, including ELOVL4, HPGD, INHBA and LAMC2, played important roles in transformation from AK to CSCC. (Ann Dermatol 30(5) 536∼543, 2018)

Bayesian Genome-wide Mapping Interacting QTL for Survival Time

Xiaojing Zhou,Dan Zhao 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.8

A Novel Function for FoxM1 in Iinterkinetic Nuclear Migration in the Developing Telencephalon and Anxiety-Related Behavior

Xiaojing Wu,Xiaochun Gu,Xiaoning Han,Ailing Du,Yan Jiang,Xiaoyun Zhang,Yanjie Wang,Guangliang Cao,Chunjie Zhao 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8

Arginine Supplementation Recovered the IFN-γ-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2α Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells

Xia, Xiaojing,Che, Yanyi,Gao, Yuanyuan,Zhao, Shuang,Ao, Changjin,Yang, Hongjian,Liu, Juxiong,Liu, Guowen,Han, Wenyu,Wang, Yuping,Lei, Liancheng Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.5

During the lactation cycle of the bovine mammary gland, autophagy is induced in bovine mammary epithelial cells (BMECs) as a cellular homeostasis and survival mechanism. Interferon gamma ($IFN-{\gamma}$) is an important antiproliferative and apoptogenic factor that has been shown to induce autophagy in multiple cell lines in vitro. However, it remains unclear whether $IFN-{\gamma}$ can induce autophagy and whether autophagy affects milk synthesis in BMECs. To understand whether $IFN-{\gamma}$ affects milk synthesis, we isolated and purified primary BMECs and investigated the effect of $IFN-{\gamma}$ on milk synthesis in primary BMECs in vitro. The results showed that $IFN-{\gamma}$ significantly inhibits milk synthesis and that autophagy was clearly induced in primary BMECs in vitro within 24 h. Interestingly, autophagy was observed following $IFN-{\gamma}$ treatment, and the inhibition of autophagy can improve milk protein and milk fat synthesis. Conversely, upregulation of autophagy decreased milk synthesis. Furthermore, mechanistic analysis confirmed that $IFN-{\gamma}$ mediated autophagy by depleting arginine and inhibiting the general control nonderepressible-2 kinase (GCN2)/eukaryotic initiation factor $2{\alpha}$ ($eIF2{\alpha}$) signaling pathway in BMECs. Then, it was found that arginine supplementation could attenuate $IFN-{\gamma}$-induced autophagy and recover milk synthesis to some extent. These findings may not only provide a novel measure for preventing the $IFN-{\gamma}$-induced decrease in milk quality but also a useful therapeutic approach for $IFN-{\gamma}$-associated breast diseases in other animals and humans.

Arginine Supplementation Recovered the IFN-gamma-Mediated Decrease in Milk Protein and Fat Synthesis by Inhibiting the GCN2/eIF2alpha Pathway, Which Induces Autophagy in Primary Bovine Mammary Epithelial Cells

Liancheng Lei,Xiaojing Xia,Yanyi Che,Yuanyuan Gao,Shuang Zhao,Changjin Ao,Hongjian Yang,Juxiong Liu,Guo-wen Liu,Wenyu Han,Yuping Wang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.5

During the lactation cycle of the bovine mammary gland, autophagy is induced in bovine mammary epithelial cells (BMECs) as a cellular homeostasis and survival mecha-nism. Interferon gamma (IFN-) is an important antiproliferative and apoptogenic factor that has been shown to induce autophagy in multiple cell lines in vitro. However, it remains unclear whether IFN- can induce autophagy and whether autophagy affects milk synthesis in BMECs. To understand whether IFN- affects milk synthesis, we isolated and purified primary BMECs and investigated the effect of IFN- on milk synthesis in primary BMECs in vitro. The results showed that IFN- significantly inhibits milk synthesis and that autophagy was clearly induced in primary BMECs in vitro within 24 h. Interestingly, autophagy was observed following IFN- treatment, and the inhibition of autophagy can improve milk protein and milk fat syn-thesis. Conversely, upregulation of autophagy decreased milk synthesis. Furthermore, mechanistic analysis con-firmed that IFN- mediated autophagy by depleting argi-nine and inhibiting the general control nonderepressible-2 kinase (GCN2)/eukaryotic initiation factor 2 (eIF2) signaling pathway in BMECs. Then, it was found that arginine supplementation could attenuate IFN--induced autophagy and recover milk synthesis to some extent. These findings may not only provide a novel measure for preventing the IFN--induced decrease in milk quality but also a useful therapeutic approach for IFN--associated breast diseases in other animals and humans.

A Pilot Study of Clinical Evaluation and Formation Mechanism of Irritable Bowel Syndrome-like Symptoms in Inflammatory Bowel Disease Patients in Remission

( Haiyang Wang ),( Xiaojing Zhao ),( Xiufang Cui ),( Meifeng Wang ),( Chunhua Jiao ),( Jiajia Li ),( Yan Yang ),( Yi Li ),( Hongjie Zhang ) 대한소화기기능성질환·운동학회 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.4

Background/Aims Some inflammatory bowel disease (IBD) patients in remission suffer from irritable bowel syndrome (IBS)-like symptoms (IBD-IBS). The pathogenesis has not yet been elucidated. The study aim is to evaluate relationships among quality of life (QOL), psychological status, and visceral sensitivity, and explore the formation mechanism of IBD-IBS. Methods Forty-seven patients with Crohn’s disease in remission, 24 ulcerative colitis in remission, 26 IBS, and 20 healthy controls were included in the study. The abdominal pain, QOL, anxiety, and depression were evaluated through questionnaires. Visceral sensitivity was measured by rectal balloon distension. The serum levels of 5-hydroxytryptamine (5-HT) and nerve growth factor (NGF) were measured by enzyme-linked immunosorbent assay. The expressions of tryptase, 5-HT, NGF, and related receptors in colonic tissues were detected by immunohistochemistry and western blot. Results Prevalence of IBS-like symptoms in Crohn’s disease and ulcerative colitis patients in clinical remission was 29.8% and 50.0%, respectively. The QOL was lower, the anxiety/depression scores were higher in IBD-IBS patients than those without IBS-like symptoms. Additionally, patients with IBD-IBS existed visceral hypersensitivity. Besides, abdominal pain was associated with poor QOL, visceral hypersensitivity, anxiety, and depression in IBD-IBS patients. The number of mast cells (MCs) and expressions of 5-HT, NGF, and related receptors were higher in IBD-IBS patients than those with no such symptoms. The serum levels of 5-HT and NGF positively correlated with abdominal pain and visceral hypersensitivity. Conclusion IBD-IBS patients may have low QOL and psychological abnormalities, as wells as visceral hypersensitivity which may be related to increased 5-HT and NGF levels released from activated mast cells. (J Neurogastroenterol Motil 2021;27:612-625)

Proteomic Analysis on Exosomes Derived from Patients’ Sera Infected with Echinococcus granulosus

Wen Wang,Xiaojing Zhou,Fang Cui,Chunli Shi,Yulan Wang,Yanfei Men,Wei Zhao,Jiaqing Zhao 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.5

Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcus granulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.

Association between hearing loss and physical performance in patients on maintenance hemodialysis

Fan Weifeng,Zhong Xiaojing,Wu Qing,Zhang Lihong,Yang Zhenhao,Gu Yong,Guo Qi,Chen Xiaoyu,Yu Chen,Zhang Kun,Ding Wei,Qi Hualin,Zhao Junli,Zhang Liming,Zhang Suhua,Niu Jianying 대한신장학회 2024 Kidney Research and Clinical Practice Vol.43 No.3

Background: The correlation between hearing loss (HL) and physical performance in patients receiving maintenance hemodialysis (MHD) remains poorly investigated. This study explored the association between HL and physical performance in patients on MHD. Methods: This multicenter cross-sectional study was conducted between July 2020 and April 2021 in seven hemodialysis centers in Shanghai and Suzhou, China. The hearing assessment was performed using pure-tone average (PTA). Physical performance was assessed using the Timed Up and Go Test (TUGT), handgrip strength, and gait speed. Results: Finally, 838 adult patients (male, 516 [61.6%]; 61.2 ± 2.6 years) were enrolled. Among them, 423 (50.5%) had mild to profound HL (male, 48.6% and female, 53.4%). Patients with HL had poorer physical performance than patients without HL (p < 0.001). TUGT was positively correlated with PTA (r = 0.265, p < 0.001), while handgrip strength and gait speed were negatively correlated with PTA (r = –0.356, p < 0.001 and r = –0.342, p < 0.001, respectively). Physical performance in patients aged <60 years showed significant dose-response relationships with HL. After adjusting for confounders, the odds ratios (95% confidence intervals) for HL across the TUGT quartiles (lowest to highest) were 1.00 (reference), 1.15 (0.73–1.81), 1.69 (1.07–2.70), and 2.87 (1.69–4.88) (p for trend = 0.005). Conclusion: Lower prevalence of HL was associated with a faster TUGT and a stronger handgrip strength in patients on MHD.

Measurement of elasticity of normal placenta using the Virtual Touch quantification technique

Size Wu,Ruixia Nan,Yueping Li,Xiaojing Cui,Xian Liang,Yanan Zhao 대한초음파의학회 2016 ULTRASONOGRAPHY Vol.35 No.3

Purpose: The aim of this study was to measure the elasticity of normal placentas using the VirtualTouch quantification (VTQ) technique. Methods: This study was approved by the Institutional Ethics Committee. Fifty randomly selected,healthy pregnant women in their second trimester and 50 randomly selected, healthy pregnantwomen in their third trimester with a single fetus were included, and their placentas underwentVTQ through shear wave velocity (SWV) measurements. The measurements were performed atdifferent locations to sample different areas of the placenta. Measurements were performed 3-4times in each location, the mean shear wave velocities were calculated without the highest andlowest values of measurements in each region, and the results were compared. Results: The SWV of the placenta was 0.983±0.260 m/sec, and the minimal and maximal speedwas 0.63 m/sec and 1.84 m/sec, respectively. There was no significant difference between thesecond and third trimester of VTQ of the placenta in terms of SWV (0.978±0.255 m/sec vs. 0.987±0.266 m/sec, P=0.711). The maternal age between second and third trimester was27.9±4.3 years and 29.2±4.4 years, respectively; there was no significant difference betweenthem (P=0.159). Conclusion: The results of this study show that the SWV of normal placenta tissue is 0.983±0.260m/sec, it has little variation between the second and third trimesters, and the VTQ technique maypotentially play an additional role in placenta evaluation.

Dynamic measurements of hydrate based gas separation in cooled silica gel

Mingjun Yang,Yongchen Song,Lanlan Jiang,Xiaojing Wang,Weiguo Liu,Yuechao Zhao,Yu Liu,Shanrong Wang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

Hydrate based gas separation is a promising method for carbon dioxide capture. The purpose of thisstudy is to analyze hydrates formation and dissociation characters when gas mixture flows throughcooled silica gel. The additives mixture (THF/SDS) was used to saturate the silica gel partly, and gasmixture (CO2/H2) was injected into it to form hydrates. Magnetic resonance imaging (MRI) images wereobtained using fast spin echo multi-slice pulse sequence. Hydrates saturations were calculatedquantitatively using MRI data. The experimental results showed that the optimal initial solutionsaturation was 34.2% in this investigation. The gas component was analyzed to assess the separationefficiency. For hydrates dissociation processes at 1 atmospheric pressure, CO2 concentrations increasedobviously. Half of the six cycles showed that more than 85.00 mol% CO2 contained in the capture gas, andthe lowest CO2 concentration was 64.83 mol%. Hydrate blockages appeared frequently, which restrictedthe contact of gas and solution and caused the incomplete transformations of residual solution tohydrates. It was a key restricted factor for hydrate based CO2 capture.