Significance of Thrombocytosis in Clinicopathologic Characteristics and Prognosis of Gastric Cancer

Li, Fang-Xuan,Wei, Li-Juan,Zhang, Huan,Li, Shi-Xia,Liu, Jun-Tian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Purpose: We aimed to study the relationship between thrombocytosis and clinical features of gastric cancerfocussing on platelet counts and gastric cancer progression through different TNM stages. Methods: According to the normal range of platelet count in our institution, 1,596 patients were divided to two groups: a thrombocytosis group (120 patients, > $400{\times}1000/{\mu}L$) and a control group (1,476 patients, ${\leq}400{\times}1000/{\mu}L$). Results: The incidence of thrombocytosis was 7.5%. Higher platelet counts were observed in patients with older age, larger tumor size, deeper invasion, lymph node metastasis, distant metastasis and advanced TNM stage. In multivariate logistic regression, tumor size, depth of tumor invasion, lymph node metastasis and TNM stage were independent risk factors for thrombocytosis of gastric cancer patients. On prognostic analysis, age, tumor size, tumor location, histologic type, depth of tumor invasion, lymph node metastasis, distant metastasis and TNM stage and platelet count were important factors. Tumor size, invasion depth, lymph node metastasis, TNM stage and the platelet count were independent prognostic factors. Conclusion: Thrombocytosis is associated with clinical features of gastric cancer patients and correlates with a poor prognosis.

Effects of different culture systems on the culture of buffalo (Bubalus bubalis) spermatogonia stem cell-like cells in vitro

Ting-Ting Li,Shuang-Shuang Geng,Hui-Yan Xu,Ao-Lin Luo,Peng-Wei Zhao,Huan Yang,Xing-Wei Liang,Yang-Qing Lu,Xiao-Gan Yang,Ke-Huan Lu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

Investigation of Hetero - Material - Gate in CNTFETs for Ultra Low Power Circuits

Wei Wang,Min Xu,Jichao Liu,Na Li,Ting Zhang,Sitao Jiang,Lu Zhang,Huan Wang,Jian Gao 대한전자공학회 2015 Journal of semiconductor technology and science Vol.15 No.1

An extensive investigation of the influence of gate engineering on the CNTFET switching, high frequency and circuit level performance has been carried out. At device level, the effects of gate engineering on the switching and high frequency characteristics for CNTFET have been theoretically investigated by using a quantum kinetic model. It is revealed that hetero - material - gate CNTFET(HMG - CNTFET) structure can significantly reduce leakage current, enhance control ability of the gate on channel, and is more suitable for use in low power and high frequency circuits. At circuit level, using the HSPICE with look - up table(LUT) based Verilog - A models, the performance parameters of circuits have been calculated and the optimum combinations of ФM1/ФM2/ФM3 have been concluded in terms of power consumption, average delay, stability, energy consumption and power - delay product(PDP). We show that, compared to a traditional CNTFET - based circuit, the one based on HMG - CNTFET has a significantly better performance (SNM, energy, PDP). In addition, results also illustrate that HMG - CNTFET circuits have a consistent trend in delay, power, and PDP with respect to the transistor size, indicating that gate engineering of CNTFETs is a promising technology. Our results may be useful for designing and optimizing CNTFET devices and circuits.

Aerodynamic properties of a streamlined bridge-girder under the interference of trains

Huan Li,Xu-hui He,Liang Hu,Xiaojun Wei 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.35 No.3

Trains emerging on a streamlined bridge-girder may have salient interference effects on the aerodynamic properties of the bridge. The present paper aims at investigating these interferences by wind tunnel measurements, covering surface pressure distributions, near wake profiles, and flow visualizations. Experimental results show that the above interferences can be categorized into two primary effects, i.e., an additional angle of attack (AoA) and an enhancement in flow separation. The additional AoA effect is demonstrated by the upward-moved stagnation point of the oncoming flow, the up-shifted global symmetrical axis of flow around the bridge-girder, and the clockwise-deflected orientation of flow approaching the bridgegirder. Due to this additional AoA effect, the two critical AoAs, where flow around the bridge-girder transits from trailing-edge vortex shedding (TEVS) to impinging leading-edge vortices (ILEV) and from ILEV to leading-edge vortex shedding (LEVS) of the bridge-girder are increased by 4° with respect to the same bridge-girder without trains. On the other hand, the underlying flow physics of the enhancement in flow separation is the large-scale vortices shedding from trains instead of TEVS, ILEV, and LEVS governed the upper half bridge-girder without trains in different ranges of AoA. Because of this enhancement, the mean lift and moment force coefficients, all the three fluctuating force coefficients (drag, lift, and moment), and the aerodynamic spanwise correlation of the bridge-girder are more significant than those without trains.

Quantum Transport Simulations of CNTFETs

Wei Wang,Huan Wang,Xueying Wang,Na Li,Changru Zhu,Guangran Xiao,Xiao Yang,Lu Zhang,Ting Zhang 대한전자공학회 2014 Journal of semiconductor technology and science Vol.14 No.5

In this paper, we explore the electrical properties and high-frequency performance of carbon nanotube field-effect transistors (CNTFETs), based on the non-equilibrium Green’s functions (NEGF) solved self - consistently with Poisson’s equations. The calculated results show that CNTFETs exhibit superior performance compared with graphene nanoribbon field-effect transistors (GNRFETs), such as better control ability of the gate on the channel, higher drive current with lower subthreshold leakage current, and lower subthreshold-swing (SS). Due to larger bandstructure-limited velocity in CNTFETs, ballistic CNTFETs present better high-frequency performance limit than that of Si MOSFETs. The parameter effects of CNTFETs are also investigated. In addition, to enhance the immunity against short - channel effects (SCE), hetero - material - gate CNTFETs (HMGCNTFETs) have been proposed, and we present a detailed numerical simulation to analyze the performances of scaling down, and conclude that HMG-CNTFETs can meet the ITRS’10 requirements better than CNTs.

Determination of calcium carbonate and styrene-butadiene latex content in the coating layer of coated paper

Huan Zhao,Yimei Hong,Qingxi Hou,Wei Liu,Yang Li,Fang Tong 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

The surface infrared spectra of coated paper with different levels of calcium carbonate and SB-latex in the coating layer were measured using ATR-FTIR. The relations between different levels of CaCO3 and SBlatex and their respective characteristic peak areas were then established. The results show that the general regression neural network (GRNN) model can be used to estimate the CaCO3 and SB-latex contents in coatings of coated papers. The maximum errors for calcium carbonate and SB-latex were only 3.32% and 3.39%, respectively, and occurred under conditions that are at the extreme of what might be encountered in an actual production facility.

Quantum Transport Simulations of CNTFETs: Performance Assessment and Comparison Study with GNRFETs

Wang, Wei,Wang, Huan,Wang, Xueying,Li, Na,Zhu, Changru,Xiao, Guangran,Yang, Xiao,Zhang, Lu,Zhang, Ting The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.5

In this paper, we explore the electrical properties and high-frequency performance of carbon nanotube field-effect transistors (CNTFETs), based on the non-equilibrium Green's functions (NEGF) solved self - consistently with Poisson's equations. The calculated results show that CNTFETs exhibit superior performance compared with graphene nanoribbon field-effect transistors (GNRFETs), such as better control ability of the gate on the channel, higher drive current with lower subthreshold leakage current, and lower subthreshold-swing (SS). Due to larger band-structure-limited velocity in CNTFETs, ballistic CNTFETs present better high-frequency performance limit than that of Si MOSFETs. The parameter effects of CNTFETs are also investigated. In addition, to enhance the immunity against short - channel effects (SCE), hetero - material - gate CNTFETs (HMG-CNTFETs) have been proposed, and we present a detailed numerical simulation to analyze the performances of scaling down, and conclude that HMG-CNTFETs can meet the ITRS'10 requirements better than CNTs.

Investigation of Hetero - Material - Gate in CNTFETs for Ultra Low Power Circuits

Wang, Wei,Xu, Min,Liu, Jichao,Li, Na,Zhang, Ting,Jiang, Sitao,Zhang, Lu,Wang, Huan,Gao, Jian The Institute of Electronics and Information Engin 2015 Journal of semiconductor technology and science Vol.15 No.1

An extensive investigation of the influence of gate engineering on the CNTFET switching, high frequency and circuit level performance has been carried out. At device level, the effects of gate engineering on the switching and high frequency characteristics for CNTFET have been theoretically investigated by using a quantum kinetic model. It is revealed that hetero - material - gate CNTFET(HMG - CNTFET) structure can significantly reduce leakage current, enhance control ability of the gate on channel, and is more suitable for use in low power and high frequency circuits. At circuit level, using the HSPICE with look - up table(LUT) based Verilog - A models, the performance parameters of circuits have been calculated and the optimum combinations of ${\Phi}_{M1}/{\Phi}_{M2}/{\Phi}_{M3}$ have been concluded in terms of power consumption, average delay, stability, energy consumption and power - delay product(PDP). We show that, compared to a traditional CNTFET - based circuit, the one based on HMG - CNTFET has a significantly better performance (SNM, energy, PDP). In addition, results also illustrate that HMG - CNTFET circuits have a consistent trend in delay, power, and PDP with respect to the transistor size, indicating that gate engineering of CNTFETs is a promising technology. Our results may be useful for designing and optimizing CNTFET devices and circuits.

Synthesis of S-adenosyl-L-methionine in Escherichia coli

Xiao-Nan Wei,Minjie Cao,Jian Li,Huan Li,Yi Song,Cuihong Du 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6

S-adenosyl-L-methionine (SAM) is an importantphysiological metabolite in vivo and may be useful inmedicines. SAM is produced from L-methionine and ATPcatalyzed by S-adenosyl-L-methionine synthetase (SAMS)in vivo. In this study, the gene encoding SAMS was clonedand a genetically engineered Escherichia coli (E. coli)BL21(pET-28a-SAMS) was constructed. The recombinantSAMS with a molecular mass of approximately 46 kDawas expressed by inducing the engineered E. coli usingisopropyl-β-D-1-thiogalactopyranoside (IPTG) as an inducer. To produce SAM using a low-cost, nontoxic and highperformanceexpression system, lactose was used as asubstitute for IPTG to induce BL21(pET-28a-SAMS). Byoptimizing the expression conditions, the concentration ofSAM produced by the engineered E. coli was 48 mg/L in theculture medium supernatant. To increase the concentrationof SAM produced, a coupled system was constructedconsisting of E. coli BL21(pET-28a-SAMS) and Saccharomycescerevisiae (S. cerevisiae) JM-310. In this coupled system,ATP generated from S. cerevisiae was provided to E. colifor producing a higher concentration of SAM. The SAMconcentration in the coupled system reached 1.7 g/L. SAMwas purified by a weak acid cationic exchange resin D113,and a simple and economical purification procedure forSAM isolation was achieved. SAM was confirmed byHigh Performance Liquid Chromatography-tandem MassSpectrometry analysis. Our study provides a feasible andconvenient approach to produce SAM.

Challenges and Advances in Materials and Fabrication Technologies of Small-Diameter Vascular Grafts

Mei‑Xian Li,Qian‑Qi Wei,Hui‑Lin Mo,Yu Ren,Wei Zhang,Huan‑Jun Lu,정윤기 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

The arterial occlusive disease is one of the leading causes of cardiovascular diseases, often requiring revascularization. Lack of suitable small-diameter vascular grafts (SDVGs), infection, thrombosis, and intimal hyperplasia associated with synthetic vascular grafts lead to a low success rate of SDVGs (< 6 mm) transplantation in the clinical treatment of cardiovascular diseases. The development of fabrication technology along with vascular tissue engineering and regenerative medicine technology allows biological tissue-engineered vascular grafts to become living grafts, which can integrate, remodel, and repair the host vessels as well as respond to the surrounding mechanical and biochemical stimuli. Hence, they potentially alleviate the shortage of existing vascular grafts. This paper evaluates the current advanced fabrication technologies for SDVGs, including electrospinning, molding, 3D printing, decellularization, and so on. Various characteristics of synthetic polymers and surface modification methods are also introduced. In addition, it also provides interdisciplinary insights into the future of small-diameter prostheses and discusses vital factors and perspectives for developing such prostheses in clinical applications. We propose that the performance of SDVGs can be improved by integrating various technologies in the near future.