돈분뇨 액비 시용이 양파의 생육, 양분흡수량 및 토양 화학성에 미치는 영향

이종태(Jong Tae Lee),하인종(In Jong Ha),김희대(Hee Dae Kim),문진성(Jin Seong Moon),김우일(Woo Il Kim),송원두(Won Doo Song) 한국원예학회 2006 원예과학기술지 Vol.24 No.2

벼와 양파 2모작 논토양에서 효율적인 돈분뇨 액비 시용을 위하여 액비 시용에 따른 양파의 생육, 양분흡수량 변화와 토양의 이화학적 특성을 조사하였다. 사용된 액비의 질소, 인산 및 칼륨 함량은 각각 4.2, 0.34, 1.2g kg⁻¹ 등이었다. 추비용 액비는 시용 10일 전에 물로 2배 희석하여 월 2회씩 4회와 6회로 나누어 시용하였다. 기비는 질소표준시비량 기준으로 19,280kg ha⁻¹로 시용하였으며 추비에 사용된 액비량은 101,910kg ha⁻¹이었다. 양파의 초장은 정식 136일 후부터 186일까지 급격히 증가하였으며 화학비료구, 액비기비+화학비료 추비구 및 액비기비+액비 4회 추비구 간에는 차이가 없었으나 무비구와 액비전량 기비구는 양분부족으로 생육이 저조하였다. 구 비대초기의 구경은 무비구에서 가장 컸으나 그 이후 화학비료구와 액비시용구에서 크게 증가하였다. 엽 생체중의 변화는 초장과 유사한 경향을 보인 반면 구 생체중은 구경과 비슷한 경향을 나타내었다. 초장과 엽중은 화학비료구와 액비전량 기비구 및 액비기비+액비 6회 추비구간 다소 작았으나 구경, 구중 및 엽수는 처리간 차이가 뚜렷하지 않았다. 양파 구와 잎의 양분흡수량은 전 생육기간에 시그모이드 형을 나타내었으며 잎과 구의 양분흡수량은 각각 잎과 구의 생육이 진전됨에 따라 증가하는 경향이었다. 화학비료구와 액비기비+화학비료 추비구 및 액비 4회 추비구간에 양분흡수량의 차이는 없었고 액비전량 기비구에서 잎의 질소와 칼륨흡수량이, 구의 질소흡수량이 화학비료구보다 적었다. 무비구는 질소, 칼륨 및 마그네슘 흡수량이 다른 처리구보다 적었다. 액비기비+액비 4회 추비구에서 질소와 인산이용률이 각각 23.5%, 13.2%로 가장 높았으나 액비기비+액비 4회 추비구에서 칼륨이용률이 높았고 상품 수량도 55.0Mg ha⁻¹로 가장 많았다. 구의 질소, 인산 및 칼륨함량은 생육이 진전됨에 감소하는 경향이었고 잎의 질소함량은 생육초기에 가장 많은 반면 칼륨함량은 구비대 초기에 가장 많았다. 수확 후 토양 pH, 질산태 질소, 교환성 칼륨 및 EC 농도는 화학비료구보다 액비시용구에서 약간 증가하였다. 본 연구 결과를 종합해 볼 때 돈분뇨 액비를 기비로 시용하고 3배 희석한 액비를 2월과 3월에 4회 추비하면 균형적인 양분공급이 가능하고 적정 수량도 확보할 수있었다. This study was carried out to evaluate the effect of liquid pig manure (LPM) on the nutrient uptake, yield of onion and soil properties. The LPM applied to onion field contained 4.2 g kg⁻¹ N, 0.34 g kg⁻¹ P, 1.2 g kg⁻¹ K and it was diluted with water by 1:2 for top-dressing, which was applied separately 4 or 6 times from February to April. The LPM application rate for basal fertilization was 19,280 kg ha⁻¹ which included 80 kg N, 6.6 kg P, and 22 kg K. The application rate for top-dressing was 101,910 kg ha⁻¹ which included 160 kg N, 13 kg P, and 53 kg K. The plant height was not almost different among treatments until 136 days after planting. Thereafter, it enlarged abruptly until 186 days, on May 4. The significant difference among CF and LPM + top-dressing by CF or LPM I was not shown, but no fertilizer and LPM only (it was applied totally as basal fertilization) made plant height of onion lessen at the maximum growth stage because of insufficient nutrients. The plant diameter increased after 136 days, and there was a significant difference between CF or LPM and no fertilizer from 186 days after planting. The leaf weight was similar tendency to plant height while bulb weight was similar to plant diameter. The amount of nutrient uptake in leaf coincided with leaf growth and the nutrient uptake in bulb swelled simultaneously with bulb growth. The difference between CF and LPM + top-dressing by CF or LPM I was not significant in nutrient uptake of onion leaf and bulb. But LPM only decreased in nitrogen and potassium of leaf and in nitrogen of bulb. And no fertilizer made a remarkable difference to others in nitrogen, potassium and magnesium of leaf and bulb. The utilization of nitrogen and phosphorus in LPM + top-dressing by CF were 23.5%, 13.2% respectively, more than the others. The LPM + top-dressing by LPM I was most available with potassium, and it leaded the most abundant marketable yield by 55.0 t ha⁻¹ as well. The nitrogen, phosphorus, and potassium contents in bulb were inclined to decrease with progress of onion growth. The nitrogen contents in leaf maximized at the initial growth stage while potassium contents increased at the initial bulbing stage. The LPM application increased pH, nitrate nitrogen, potassium and EC in soil more than chemical fertilizer (CF) did.

Regeneration of Bovine Mammary Gland in Immunodeficient Mice by Transplantation of Bovine Mammary Epithelial Cells mixed with Matrigel

Woo Tae Ha1,Hyun Jung Park,Won-Young Lee,Ha Yeon Jeong,Hyuk Song 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. MAC-T cells (1×107) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

The Comparisional Analysis of Colostrum Protein between the First and Third Days after Calving using Proteomic Analysis

Woo Tae Ha1,Hyun Jung Park,Won-Young Lee,Ha Yeon Jeong,Hyuk Song 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

The colostrum proteins are an important energy source for newborns and improves their innate immune system. Recently, there are many interest about beneficial factors in colostrum to health and many products using colostrum are attentive into dietary supplements in global industry. The aim of this study was to compare the enriched proteins between the colostrum from the first and the third day after calving using proteomic analysis and to analyze which enriched protein will be useful to industry of dietary supplements. In this study, cows in the experimental group were fed a standard composition of feed for 12 months, after which we collected the colostrum on the first and the third day after calving. By comparison between the first and the third day colostrum, several factors, including beta-lactoglobulin, fibrinogen gamma-B chain, complement C3, zinc-alpha-2 glycoprotein, bP47 protein, beta casein, and alpha-S2 casein were enriched in the third day colostrum, whereas immunoglobulin gamma 1 and beta- casein A2 were enriched in the first day colostrum. The results suggest that the colostrum composition depends on time and the first day colostrum is important to establish the primary specific immune system, whereas the third day colostrum might regulate the non-specific immune system and increase nutrition using casein and the third day colostrum might be useful dietary products for supporting the immune system.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

荒廢山地에서의 산불이 森林植生및 土壤에 미치는 影響에 관한 硏究(III)

禹保命,權台鎬,李峻雨,金景河 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.2

The third year's results on effects of surface fire on frest vegetations and soil properties were investigated in Mt.Gwanak, Kyunggi-do successively after the study of the first year's effects in the same place. Organic matters in soil had increased just after fire and whatsome decreased during 1 year after fire, and resulted in percentage that was less than unfired area in the third year after fire. The pH of subsoil was continuously increasing from just after fire till after 1 year, but decreased somewhat in the third year after fire. More exact classification into Increasers, Decreasers, Invaders and Neutral species was possible in the third year's study. According to comparison of similarities, the vegetational structure of the species at fired area has been gradually restoring to original state as time goes by.

冠岳樹木圓地域內 荒廢山地土壤의 肥沃化를 통한 綠化促進에 關한 硏究(II)

禹保命,權台鎬,李宗學,金景河,李峻雨,麻鎬燮 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.2

The shallow soil depth and severe rock exposures on the hillslopes in the Kwanak aboretum area which resulted from the heavy soil erosion have made very poor growth of forest vegetation. In order to establish the forest vegetation in this area, it is urgent to recover the fertility of soil and the productivity of existing trees. For this reason, fertilization experiment (using the briquet compound fertilizer) was conducted on summer and autumn season in 1983 with the 4 main native species (Pinus densiflora, Pinus rigida, Juniperus rigida, Rhododendron schlippenbachii) growing on these eroded hillslopes. Result in 1985 and 1986 of this experiment represented that the fertilization was effective toward both height and root-collar diameter growth of trees and spring fertilization was, relatively, more effective than autumn fertilization. It also represented that effects of fertilization to P. rigida were more than those to other species. Therefore, besides the engineering methods and afforestation measures for soil erosion control, rapid establishment of vegetation through conservation and recovery of existing trees by fertilization is available measures for the rehabilitation of rockily eroded hillslopes like Mt. Kwanak area.

DMA 가스 검지를 위한 ZnO 박막의 aging 효과

김성우,최혁환,이명교,권태하 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

The DMA(Dimethylamine) gas sensors were fabricated with the ZnO-based thin films grown by a RF magnetron sputtering method. The characteristics of the sensors to aging conditions were investicated. The sensors with ZnO-based thin films aged for 72hrs at a temperature of 330 ℃ in oxygen showed high sensitivity, low working temperature and good linearity. The sensors with ZnO-based thin film doped with 0.1 wt.% Pt catalytics and aged for 72hrs at a temperature of 330 ℃ in oxygen showed the maximum sensitivity of 90 at a working temperature of 150 ℃ and to DMA gas of 160 ppm. The sensor was electrically stablized.

ZnO 박막 센서의 암모니아 가스 검지 특성

최우창,김성우,류지열,박성현,최혁환,이명교,권태하 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1

The ammonia gas sensors were fabricated with the ZnO-based thin films grown by a RF magnetron sputtering method. We investigated the sensitivity and response time according to temperature variation and ammonia gas concentration. The sensors sputtered in oxygen atmosphere showed higher sensitivity than those sputtered in argon atmosphere. The Au(0.3 wt.%) doped-ZnO thin film sensors aged at 330 ℃ showed the maximum sensitivity of 28 and good response time at a working temperature of 250 ℃ and to 160 ppm ammonia gas. The Pt(0.1 wt.%) doped-ZnO thin film sensors showed the maximum sensitivity at a low working temperature of 200 ℃.

ZnO 박막 센서의 DMA 가스 검지 특성

김성우,최우창,류지열,박성현,최혁환,이명교,권태하 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1

The DMA(Dimethylamine) gas sensors were fabricated with the ZnO-based thin films grown by a RF magnetron sputtering method. We investigated the sensitivity and response time according to temperature variation and DMA gas concentration. The ZnO-based thin film sensors sputtered in oxygen atmosphere showed higher sensitivity than those sputtered in argon atmosphere. The ZnO-based thin film sensors doped with Al_(2)O_(3), In_(2)O_(3) and V_(2)O_(5) and sputtered in oxygen atmosphere showed the maximum sensitivity of 218(working temperature, 250 ℃, DMA gas, 160 ppm) and speedy response time. The ZnO-based thin film sensors doped with Al_(2)O_(3), In_(2)O_(3), TiO_(2) and V_(2)O_(5), sputtered in oxygen atmosphere and aged at 330 ℃ showed the maximum sensitivity of 156(working temperature, 250 ℃, DMA gas, 160 ppm).

개인선량계 교정을 위한 환산인자 계산

이태영,이원구,하정우 대한방사선 방어학회 1991 방사선방어학회지 Vol.16 No.1

MCNP 코드를 사용하여 IAEA에서 권고하고 있는 물팬톰과 미국내 선량계 성능시험 프로그램에서 규정하고 있는 PMMA 팬톰내 0.07mm 및 10mm 깊이에서의 환산인자 H(d)/Ka을 계산하였다. 계산은 팬톰의 한면에 수직으로 입사하는 단일에너지 광자의 확장정열빔에 대해 수행하였다. 결과는 팬톰내에서 정의되는 선량당량으로 선량계를 교정할 때 환산인지로 사용될 수 있다. MCNP code was used to calculate conversion factor H(d)/Ka at the depths of 0.07 and 10mm within a water phantom recommended by IAEA and within a PMMA phantom required by the US dosimeter proficiency testing programmes. The calculations were performed for an expanded parrallel beam of monoenergetic photons of perpendicular incidence on one faces of the phantom. The results can be used as conversion factor in calibrating individual dosemeters in terms of the dose equivalent quantities defined directly in the phantom.