Single-Port Robotic Cholecystectomy: Early Experience from 8 Cases

( Hyung Jun Kwon ),( Horyon Kong ),( Sang Geol Kim ),( Yun Jin Hwang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Single-port laparoscopic cholecystectomy (SPLC) is a technical concept to reduced pain and improve cosmetic results when compared to multi-port cholecystectomy. However, SPLC is associated with technical limitation due to the enhanced complexity of the approach and limited number of specialized instruments or platforms. On the other hand, using a robotic platform may overcome these problems and enable more precise surgical actions by increasing freedom of movement and by restoring intuitive instrument control. In this presentation, we report the early clinical experience of our first 8 sing-port robotic cholecystectomy (SPRC) cases. Methods: Between November 2016 and February 2017, eight patients underwent SPRC with the da Vinci Xi robot and single-site instrumentation. We retrospectively reviewed clinical data on those patients. Results: All of 8 patients had completion of SPRC. Seven patients were female and one was male. Average patient age was 43.3±11.8 years and BMI was 22.4±1.4 kg/m2. Three patients (37.5%) were diagnosed with chronic calculous cholecystitis. Three patients (37.5%) underwent operation for polypoid lesions of the gallbladder. One patient (12.5%) was diagnosed with acute calculous cholecystitis. The mean operation time (skin-to-skin) was 83.9±30.7 min, docking time was 13.25±8.1 min, and console time was 42.1±26.4 min. The intraoperative blood loss was negligible. The mean Visual Analog Pain Scale score 6hr after the surgery was 2.9±0.4. The mean length of hospital stay average postoperative hospital stay was 2.3±1.0 day. There were no intraoperative complication and one patient developed seroma on port site. Conclusions: Robotic single-port cholecystectomy appears feasible and safe in our early experience.

Impact of postoperative duration of Aspirin use on longevity of bioprosthetic pulmonary valve in patients who underwent congenital heart disease repair

Hwang, Tae-Woong,Kim, Sung-Ook,Lee, Sang-Yun,Kim, Seong-Ho,Choi, Eun-Young,Jang, So-Ick,Park, Su-Jin,Kwon, Hye-Won,Lim, Hyo-Bin,Lee, Chang-Ha,Choi, Eun-Seok The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.11

Purpose: Generally, aspirin is used as a protective agent against thrombogenic phenomenon after pulmonary valve replacement (PVR) using a bioprosthetic valve. However, the appropriate duration of aspirin use is unclear. We analyzed the impact of postoperative duration of aspirin use on the longevity of bioprosthetic pulmonary valves in patients who underwent repair for congenital heart diseases. Methods: We retrospectively reviewed the clinical data of 137 patients who underwent PVR using a bioprosthetic valve between January 2000 and December 2003. Among these patients, 89 were included in our study and divided into groups I (${\leq}12$ months) and II (>12 months) according to duration of aspirin use. We analyzed echocardiographic data from 9 to 11 years after PVR. Pulmonary vale stenosis and regurgitation were classified as mild, moderate, or severe. Results: The 89 patients consisted of 53 males and 36 females. Their mean age was $14.3{\pm}8.9$ years (range, 2.6-48 years) and body weight was $37.6{\pm}14.7kg$ (range, 14-72 kg). The postoperative duration of aspirin use was $7.3{\pm}2.9$ months in group I and $32.8{\pm}28.4$ months in group II. However, no significant difference in sex ratio, age, body weight, type of bioprosthetic valve, and number of early redo-PVRs. In the comparison of echocardiographic data about 10 years later, no significant difference in pulmonary valve function was found. The overall freedom rate from redo-PVR at 10 years showed no significant difference (P=0.498). Conclusion: Our results indicated no benefit from long-term aspirin medication (>6 months) in patients who underwent PVR with a bioprosthetic valve.

A case of cervical chondrocutaneous branchial remnants

( Sang Hyeon Hwang ),( Jae Yun Lim ),( Ju Yeon Choi ),( Han Saem Kim ),( Jung In Kim ),( Jung Min ),( Hyun Min Seo ),( Ho Joo Jung ),( Jae Hui Nam ),( Ga Young Lee ),( Won Serk Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

Cervical chondrocutaneous branchial remnants are rare developmental anomalies of the brachial apparatus which appear as a firm, protruding papule or nodule on the neck along the line of anterior border of the sternocleidomastoid muscle. Primitive branchial embryonic tissues migrate into the final lateral facial position to form the adult derivatives of head and neck. However, failure of proper migration, fusion, or maturation of the branchial apparatus components results in a variety of congenital defects. Most branchial cysts and fistulae are considered to be derived from the first and second branchial clefts. Of these, cervical chondrocutaneous branchial remnants are infrequent, whereas branchial cysts and sinuses are relatively more common. Our patient, a 9-month-old girl, presented with a solitary skin-colored about 0.4 cm sized soft papule on the lower third of the left neck. The lesion had been presented at birth and physical examination revealed no remarkable findings except for the skin lesion. The histopathologic examination revealed normal epidermis and characteristic cartilage core in the dermis without connection to the epidermis. The histopathological features were consistent with cervical chondrocutaneous branchial remnants.

Reducing Effect of IL-32α in the Development of Stroke Through Blocking of NF-κB, but Enhancement of STAT3 Pathways

Hwang, Chul Ju,Yun, Hyung-Mun,Jung, Yu Yeon,Lee, Dong Hun,Yoon, Na Young,Seo, Hyun Ok,Han, Jin-Yi,Oh, Ki-Wan,Choi, Dong Young,Han, Sang-Bae,Yoon, Do Young,Hong, Jin Tae Springer-Verlag 2015 Molecular Neurobiology Vol.51 No.2

Theoretical approach for the mechanical behaviors of conical composite tubes considering fiber orientation errors under static loads

Hwang, Sang-Kyun,Hwang, Hui-Yun Elsevier 2018 Composite structures Vol.183 No.-

<P><B>Abstract</B></P> <P>This study analyzes the mechanical behaviors of conical composite tubes, considering fiber orientation errors, under static loads. Fiber orientation errors are defined as fiber orientation changes that occur due to the taper angle of conical composite tubes during the wrapping process. Such fiber orientation errors are one of many important parameters in predicting mechanical properties, and must be taken into account for the design of conical composite tubes. Thus, this study derived the theoretical equations to analyze the mechanical behaviors of conical composite tubes under static loads considering fiber orientation errors according to the laminate plate theory. For verification of the derived equations, the calculation results were compared with the results of other research that did not take fiber orientation errors into account as well as experimental results that did include fiber orientation errors. As a result, the derived equations could predict the static behaviors of conical composite tubes 5.5% errors. Also, we performed parametric studies such as taper angles, sizes, and stacking sequences. The effect of taper angles on the static behaviors was the most important and the static deformations with and without the consideration of fiber orientation errors had a 500% difference over 10<SUP>O</SUP> of the taper angle.</P>

HCV, Alcoholic : O-024 ; The association between IL28B polymorphism and the progression of liver fibrosis in patients with chronic hepatitis C

( Yun Ji Park ),( Sun Young Hwang ),( Sun Pyo Hong ),( Soon Ho Um ),( Hong Sik Lee ),( Hoon Jai Chun ),( Chang Duck Kim ),( Ho Sang Ryu ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: The genetic variation of IL28B gene on chromosome 19 has relevance to spontaneous seroclearance and virologic response to the interferon based standard therapy of chronic hepatitis C. Up to date, however, it is not clear whether these polymorphisms of IL28B are related with disease progression of chronic hepatitis C. In this study, we aimed to determine the association between IL28B genotype and the stage of fibrosis in patients with chronic hepatitis C. Methods: A total of 189 patients seropositive for anti-HCV were enrolled, 10 of whom had been spontaneously recovered from infection. Two SNP polymorphism of IL28B, rs8099917 and rs12979860 were investigated using RFMP genotyping and sequencing. The degree of liver fibrosis was determined by liver biopsy in 91 patients who had no definite evidence of cirrhosis in imaging studies. Results: In the whole series, rs12979860 CC, CT, TT genotype were found in 85.7%, 13.8%, 0.5% of patients, respectively, while rs8099917 genotype TT, TG, GG, in 86.2%, 13.2%, 0.5%, respectively. The rs12979860 CC genotype and reciprocative rs8099917 TT genotype were found in 154 of 179 patients with chronic HCV infection and in 9 of 10 spontaneously serocleared patients (86.0% vs. 90.0%, p=0.925). rs12979860 CC genotype was identified in 84.3% of patients with mild to moderate fibrosis (F0-F2) while in 87.7% of patients with advanced fibrosis (F3-4) (p=0.551). In rs8099917 TT genotype, each of them was 85.7% and 86.4% (p=0.901). In multivariate logistic regression analysis, the only significant factor associated with advanced liver fibrosis was age (OR 4.733, p<0.001). Conclusions: In the present study, IL28B polymorphisms (major alleles, rs12979860 CC and rs8099917 TT) were associated neither with spontaneous seroclearance nor with the progression of fibrosis. However, the small sample size of the present study warrants further prospective multicenter study to clearly the role of SNPs in the progression of chronic hepatitis C.

Immunohistochemical Staining to Identify Concomitant Systemic Mastocytosis in Acute Myeloid Leukemia with RUNX1::RUNX1T1

Hwang Sang Mee,Kim Beom Joon,Lee Jee-Soo,Seong Moon-Woo,Seo Soo Hyun,Paik Jin Ho,Kim Sang-A,Lee Ji Yun,Lee Jeong-Ok,Chang Yoon Hwan,Bang Soo Mee 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.6

Systemic mastocytosis with associated hematological neoplasm (SM-AHN) poses diagnostic challenges because of the coexistence of atypical mast cell proliferation and hematological neoplasms. We assessed the presence of SM-AHN in patients with acute myeloid leukemia (AML) with RUNX1::RUNX1T1 from 2014 to 2020. Bone marrow (BM) samples were evaluated for mast cell aggregates using CD117 and CD25 immunohistochemical (IHC) staining. The KIT D816V variant burden at diagnosis and post induction was assessed using droplet digital PCR. Among 23 patients diagnosed as having AML with RUNX1::RUNX1T1, four (17.4%) were also diagnosed as having SM-AHN. No significant differences in clinical characteristics or overall survival (P=0.565) were observed between patients with or without SM-AHN, except for the presence of KIT variants (P=0.040). After induction therapy, IHC staining revealed the presence of mast cell aggregates in the BM, and the KIT D816V variant burden decreased with decreasing blast count and was similar in BM aspirates, smear slides, and sections. Concomitant SM-AHN was not infrequent in AML patients with RUNX1::RUNX1T1. This study showed the importance of CD117 and CD25 IHC staining after induction chemotherapy for SM-AHN screening, especially in patients with KIT variants.

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells

Yun, Hong Shik,Baek, Jeong-Hwa,Yim, Ji-Hye,Um, Hong-Duck,Park, Jong Kuk,Song, Jie-Young,Park, In-Chul,Kim, Jae-Sung,Lee, Su-Jae,Lee, Chang-Woo,Hwang, Sang-Gu TaylorFrancis 2016 Cancer Biology & Therapy Vol.17 No.2

<P><B>ABSTRACT</B></P><P>Tumor cell radioresistance is a major contributor to radiotherapy failure, highlighting the importance of identifying predictive biomarkers for radioresistance. In this work, we established a radioresistant H460 (RR-H460) cell line from parental radiosensitive H460 lung cancer cells by exposure to fractionated radiation. The radiation-resistant, anti-apoptotic phenotype of RR-H460 cell lines was confirmed by their enhanced clonogenic survival and increased expression of the radioresistance genes Hsp90 and Her-3. RR-H460 cells displayed characteristics of cancer stem-like cells (CSCs), including induction of the surface marker CD44 and stem cell markers Nanog, Oct4, and Sox2. RR-H460 cells also exhibited sphere formation and malignant behavior, further supporting a CSC phenotype. Using proteomic analyses, we identified 8 proteins that were up-regulated in RR-H460 CSC lines and therefore potentially involved in radioresistance and CSC-related biological processes. Notably, 4 of these—PAI-2, NOMO2, KLC4, and PLOD3—have not been previously linked to radioresistance. Depletion of these individual genes sensitized RR-H460 cells to radiotoxicity and additively enhancing radiation-induced apoptosis. Our findings suggest the possibility of integrating molecular targeted therapy with radiotherapy as a strategy for resolving the radioresistance of lung tumors.</P>

Effect of natural pre-converted nitrite sources on color development in raw and cooked pork sausage

Hwang, Ko-Eun,Kim, Tae-Kyung,Kim, Hyun-Wook,Seo, Dong-Ho,Kim, Young-Boong,Jeon, Ki-Hong,Choi, Yun-Sang Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: The effect of pre-converted nitrites from natural sources (spinach, lettuce, celery, and red beet) on color development in raw and cooked pork sausage was investigated in this study. Methods: The pork sausage was manufactured with six treatments: NC (negative control, nitrite free), PC (positive control, 150 ppm sodium nitrite), FS (3.0% fermented spinach extracts), FL (3.0% fermented lettuce extracts), FC (3.0% fermented celery extracts), and FR (3.0% fermented red beet extracts). Results: The pH value of the pre-converted nitrites groups was lower than those treated with 150 ppm sodium nitrite (p<0.05). The color values of raw and cooked pork sausage added with pre-converted nitrite showed slightly lower and/or similar lightness, lower redness, and higher yellowness values than PC. Color development (redness values) of cooked samples added with FS was higher than those of the NC and other treatments (FL, FC, and FR). Additionally, treatments with FS and FL were most effective for reducing thiobarbituric acid reactive substances and volatile basic nitrogen than the NC. Conclusion: Effects of natural nitrites from fermented vegetables on shelf stability of raw and cooked pork sausages were investigated. Fermented spinach extract was much more useful for maintaining the color development, but also inhibiting lipid and protein oxidation of cooked pork sausage. Therefore, pre-converted nitrite from spinach as a natural nitrite could be used as another natural nitrite source for making processed meat products.

Association between Hepatic Steatosis and the Development of Hepatocellular Carcinoma in Patients with Chronic Hepatitis B

( Yun Bin Lee ),( Yeonjung Ha ),( Young Eun Chon ),( Mi Na Kim ),( Joo Ho Lee ),( Hana Park ),( Kyu Sung Rim ),( Seong Gyu Hwang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: As nonalcoholic fatty liver disease becomes a worldwide epidemic, hepatic steatosis has been frequently demonstrated in chronic hepatitis B patients without excessive alcohol intake. Whether presence of hepatic steatosis in these patients is associated with higher risk of developing hepatocellular carcinoma (HCC) has not been well established. We investigated the impact of histologically proven hepatic steatosis on the risk of HCC development. Methods: Chronically hepatitis B virus-infected patients without significant alcohol consumption, who underwent liver biopsy from January 2007 to December 2015 in a single centre, were consecutively included. Patients were categorised into two groups according to the presence or absence of hepatic steatosis ≥ 5%, and the association of steatosis with subsequent HCC risk was analysed. To adjust for differences in patient characteristics including metabolic features, inverse probability weighting (IPW) using propensity scores was used. Results: Hepatic steatosis was histologically proven in 70 patients (21.8%) among a total of 321 patients. During the median (interquartile range) follow-up of 5.3 (2.9-8.3) years, 17 of 321 patients (5.3%) developed HCC: 9 of 251 patients (3.6%) without steatosis and 8 of 70 patients (11.4%) with steatosis. The 5-year cumulative incidences of HCC were 1.9% and 8.2% among patients without steatosis and with steatosis, respectively (P=.004) (Figure A). Steatosis was associated with higher risk of HCC development (adjusted hazards ratio [HR], 3.005; 95% confidence interval [CI], 1.122-8.051; P=.029). After balancing with IPW, the incidences of HCC were not significantly different between the groups (P=.187) (Figure B), and the association between steatosis and HCC development was not significant (adjusted HR, 2.292; 95% CI, 0.693-7.587; P=.17). Conclusions: Hepatic steatosis was associated with higher risk of developing HCC in patients with chronic hepatitis B. However, the association of steatosis per se with HCC development was not evident after adjustment for metabolic characteristics.