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Seon Yeong Ji,Min Yeong Kim,So Yung Kim,Hyun Hwangbo,Hyesook Lee,Suhkmann Kim,Yung Hyun Choi 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Previous studies have suggested that 5-aminolevulinic acid (ALA), a heme precursor, may be a potential treatment for inflammatory responses and oxidative stress. In this study, we investigated the inhibitory effect of ALA on inflammatory and oxidative stress activated by lipopolysaccharide (LPS) in RAW 264.7 macrophages and zebrafish models. Our results indicated that ALA treatment significantly attenuated the LPS-induced release of pro-inflammatory mediators including nitric oxide (NO) and prostaglandin E2, which was associated with decreased inducible NO synthase and cyclooxygenase-2 expression. ALA also inhibited the LPS-induced expression of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6, reducing their extracellular secretion. Additionally, ALA abolished reactive oxygen species (ROS) generation, improved the mitochondrial mass, and enhanced the expression of heme oxygenase-1 (HO-1) and the activation of nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in LPS-stimulated RAW 264.7 macrophages. However, zinc protoporphyrin, a specific inhibitor of HO-1, reversed the ALA-mediated inhibition of pro-inflammatory cytokines production and activation of mitochondrial function in LPS-treated RAW 264.7 macrophages. Furthermore, ALA significantly abolished the expression of LPS-induced pro-inflammatory mediators and cytokines, and showed strong protective effects against NO and ROS production in zebrafish larvae. In conclusion, our findings suggest that ALA exerts LPS-induced anti-inflammatory and antioxidant effects by upregulating the Nrf2/HO-1 signaling pathway, and that ALA can be a potential functional agent to prevent inflammatory and oxidative damage.
Change of Blood Cells` Number from Capillaries and Venous Blood of 20`s Healthy Adults
( Ji-yeong Seon ),( Jae Sik Lee ) 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.4
It was confirmed that we got somewhat different results even though we performed same items with same methods from capillaries and venous blood of healthy 72 cases. Items which capillaries blood has higher value than venous blood are the numbers of erythrocyte, lymphocyte, basophil, hematocrit, MCHC and RDW. Total numbers of RBC (p<0.035), lymphocyte and basophil are shown statistically significance. Items which venous blood has higher value than capillary blood are the numbers of platelet (p<0.00) and neutrophil (p<0.01). Fallible items in clinics can be shown the numbers of RBC, platelet, lymphocyte and neutrophil because we got somewhat different results even though we performed same items with same methods from capillaries and venous blood, respectively. It is necessary to choose the clear criteria and normal value depends on clinical specimen as the number of platelets are measured with an abnormal value shown over 40% down. As normal difference according to each item and method is currently acceptable and applicable in clinics, it is considered that a new normal value depends on clinical specimen should be established and is to be useful positively in clinics.
( Seon Yeong Ji ),( Hee-Jae Cha ),( Ilandarage Menu Neelaka Molagoda ),( Min Yeong Kim ),( So Young Kim ),( Hyun Hwangbo ),( Hyesook Lee ),( Gi-Young Kim ),( Do-Hyung Kim ),( Jin Won Hyun ),( Heui-Soo 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.6
In this study, we investigated the inhibitory effect of 5-aminolevulinic acid (ALA), a heme precursor, on inflammatory and oxidative stress activated by lipopolysaccharide (LPS) in RAW 264.7 macrophages by estimating nitric oxide (NO), prostaglandin E2 (PGE2), cytokines, and reactive oxygen species (ROS). We also evaluated the molecular mechanisms through analysis of the expression of their regulatory genes, and further evaluated the anti-inflammatory and antioxidant efficacy of ALA against LPS in the zebrafish model. Our results indicated that ALA treatment significantly attenuated the LPS-induced release of pro-inflammatory mediators including NO and PGE2, which was associated with decreased inducible NO synthase and cyclooxygenase-2 expression. ALA also inhibited the LPS-induced expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, reducing their extracellular secretion. Additionally, ALA abolished ROS generation, improved the mitochondrial mass, and enhanced the expression of heme oxygenase-1 (HO-1) and the activation of nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in LPS-stimulated RAW 264.7 macrophages. However, zinc protoporphyrin, a specific inhibitor of HO-1, reversed the ALA-mediated inhibition of pro-inflammatory cytokines production and activation of mitochondrial function in LPS-treated RAW 264.7 macrophages. Furthermore, ALA significantly abolished the expression of LPS-induced pro-inflammatory mediators and cytokines, and showed strong protective effects against NO and ROS production in zebrafish larvae. In conclusion, our findings suggest that ALA exerts LPS-induced anti-inflammatory and antioxidant effects by upregulating the Nrf2/HO-1 signaling pathway, and that ALA can be a potential functional agent to prevent inflammatory and oxidative damage.
Change of Blood Cells' Number from Capillaries and Venous Blood of 20's Healthy Adults
Seon, Ji-Yeong,Lee, Jae Sik 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.4
It was confirmed that we got somewhat different results even though we performed same items with same methods from capillaries and venous blood of healthy 72 cases. Items which capillaries blood has higher value than venous blood are the numbers of erythrocyte, lymphocyte, basophil, hematocrit, MCHC and RDW. Total numbers of RBC (p<0.035), lymphocyte and basophil are shown statistically significance. Items which venous blood has higher value than capillary blood are the numbers of platelet (p<0.00) and neutrophil (p<0.01). Fallible items in clinics can be shown the numbers of RBC, platelet, lymphocyte and neutrophil because we got somewhat different results even though we performed same items with same methods from capillaries and venous blood, respectively. It is necessary to choose the clear criteria and normal value depends on clinical specimen as the number of platelets are measured with an abnormal value shown over 40% down. As normal difference according to each item and method is currently acceptable and applicable in clinics, it is considered that a new normal value depends on clinical specimen should be established and is to be useful positively in clinics.
Seon Yeong Ji,Min Yeong Kim,So Yung Kim,Hyun Hwangbo,Da Hye Kim,Hyesook Lee,Joung-Hyun Park,Bae-Jin Lee,Yung Hyun Choi 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Numerous studies have been reported that excessive salt intake is associated with increased risk of hypertension, renal failure and cardiovascular events. Recently, several gamma-aminobutyric acid (GABA) supplements have been shown to reduced high salt-induced diseases. Although there is suggested that GABA salt (GS), a fermented salt by Lactobacillus brevis BJ20 containing GABA, attenuated high salt-induced hypertension, the effect of GS on the organs is unclear. The aim of this study was to elucidate the effect of oral administration of GS, GS containing live lactic acid bacteria (LGS) and GS containing post-biotics (PGS) on organ weight, hematology and clinical parameters in high salt-fed C57BL/6 Mice. Normal group was orally administered with 0.3% NaCl, and high salt groups were fed with salt, GS, LGS and PGS containing 4% NaCl for 8 weeks. High salt intake had no effect on the body weight and hematological profiles. However, the weights of spleen and heart were markedly decreased in high salt groups, while liver, lung and pancreas were not altered. In kidney, the expressions of T-cell immunoglobulin mucin domain-1 and inflammatory cytokines were greatly increased in high salt mice, but attenuated by GS administration, suggesting GS have biological safety and prevent high salt intake-mediated disease.
RAW 264.7 대식세포에서 환원형 glutathione인 luthione의 면역 증강 활성 평가
지선영(Seon Yeong Ji),권다혜(Da Hye Kwon),황혜진(Hye Jin Hwang),최영현(Yung Hyun Choi) 한국생명과학회 2023 생명과학회지 Vol.33 No.5
항산화제로서 산화적 손상의 방지에 중요한 역할을 하는 것으로 알려진 glutathione (GSH)의 면역 조절에 대한 연구는 현재까지 제대로 이루어지지 않았다. 본 연구에서 우리는 환원형 GSH인 luthione<SUP>®</SUP>이 RAW 264.7 세포에서 면역 강화 효과가 있는지를 조사하였다. 유세포 분석 및 면역 형광 실험의 결과에 의하면, luthione은 대조군 세포에 비해 대식세포의 대표적인 기능인 식세포 활성을 luthione 처리 농도 의적으로 증가시키는 것으로 나타났다. 또한, cytokine array의 결과에 의하면, IL-5, IL-1β와 IL-27의 발현이 luthione이 처리된 세포에서 유의하게 증가하였다. 아울러 luthione에 의한 TNF-α 및 IL-1β의 생성 증가는 그들의 단백질 발현 증가를 통해 이루어졌으며, NO 및 PGE2와 같은 면역 매개체 유리의 증가는 iNOS 및 COX-2의 발현 증가와 관련이 있었으며, 이는 M1 대식세포 분화 마커인 CD86 발현의 증가와 연관성이 있었다. 그리고 heatmap 분석을 통하여 SOCS1/3 매개 STAT/JAK 신호 전달 경로가 luthione에 의한 면역 조절 증가에 관여함을 확인하였다. 결론적으로, 우리의 결과는 luthione이 M1 macrophage polarization의 분자 조절자로 작용하여 면역 능력을 향상시킬 수 있음을 시사한다. Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione<SUP>®</SUP>, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E2 was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.