강산 및 강알칼리 음독에서 냉각 용액을 사용한 희석 요법과 중화 요법의 가능성

이중의,송형곤,김동훈,권운용,곽영호,서길준,윤여규 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: There is no effective treatment modality for caustic agent ingestion. Dilution and neutralization are prohibited because of the risk of secondary thermal injury. This experiment is designed to evaluate the amount of dilution and neutralization heat and to gauge the applicability of dilution and neutralization therapy using cold solutions to suppress the peak temperature. Methods: This is an in-vitro chemical experiment. HCl, CH3COOH, NaOH, and NH4OH are selected as representatives of strong and weak acids and strong and weak alkali, respectively. 20℃, 11.6M, 5.8M, and 2.9M solutions of each acid and alkali are made and mixed using a magnetic stirrer at a room air temperature of 28℃. The peak temperature, the duration above 40℃, and the heat amount are measured or calculated. Results: When a 11.6M HCl or NaOH solution is diluted with same amount of water, 32 or 18cal. per mL of HCl or NaOH is produced, respectively. HCl produces a significant peak temperature, but NaOH does not. The lower the concentration, the lower the amount of heat production. 11.6M CH3COOH and NH4OH solutions don't produce dilution heat.11.6M and 5.8M solutions of all acids and alkali produce destructive neutralization heat. However, 2.9M solutions produce neutralization heat which might be controllable. When a 11.6M HCl or NaOH solution is neutralized with a -10℃ 2.9 M NaOH or HCl solution, respectively, the peak temperature produced is below 40℃ and seems to add little thermal damage to viable tissue. Conclusion: Dilution and neutralization with a cold solution in cases of strong acid or alkali ingestion is a promising method to avoid thermal injury.

Epidermal growth factor improves the migration and contractility of aged fibroblasts cultured on 3D collagen matrices

KIM, DAEHWAN,KIM, SO YOUNG,MUN, SEOG KYUN,RHEE, SANGMYUNG,KIM, BEOM JOON UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.35 No.4

<P>Epidermal growth factor (EGF) plays a critical role in fibroblasts by stimulating the production of collagen and supports cell renewal through the interaction between keratinocytes and fibroblasts. It is well known that the contractile activity of fibroblasts is required for the remodeling of the extracellular matrix (ECM), which contributes to skin elasticity. However, the role of EGF in the contraction of aged fibroblasts under 3-dimensional (3D) culture conditions is not yet fully understood. In the present study, we demonstrated that young fibroblasts spread and proliferated more rapidly than aged fibroblasts under 2-dimensional (2D) culture conditions. Cell migration assay using a nested collagen matrix revealed that the migration of young fibroblasts was also greater than that of aged fibroblasts under 3D culture conditions. However, the addition of recombinant human EGF (rhEGF) resulted in the enhanced migration of aged fibroblasts; the migration rate was similar to that of the young fibroblasts. The aged fibroblasts showed decreased cluster formation compared with the young fibroblasts on the collagen matrix, which was improved by the addition of rhEGF. Furthermore, cell contraction assay revealed that the basal contractility of the aged fibroblasts was lower than that of the young fibroblasts; however, following treatment with rhEGF, the contractility was restored to levels similar or even higher to those of the young fibroblasts. Taken together, our results suggest that rhEGF is a potential renewal agent that acts to improve the migration and contraction of aged fibroblasts more efficiently than young fibroblasts under 3D culture conditions; thus, EGF may have valuable regenerative effects on aged skin.</P>

CAT score and SGRQ definitions of chronic bronchitis as an alternative to the classical definition

( Joon Young Choi ),( Chin Kook Rhee ),( Hyoung Kyu Yoon ),( Kyeong-cheol Shin ),( So-young Park ),( Chang Youl Lee ),( Seung Won Ra ),( Ki Suck Jung ),( Kwang Ha Yoo ),( Chang-hoon Lee ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Purpose: Previous studies have used various definitions to classify chronic obstructive pulmonary disease (COPD) patients into chronic bronchitis (CB) and non-CB patients. This study was performed to identify differences among three definitions of CB based on the classical method, St. George’s Respiratory Questionnaire (SGRQ), and the CAT (COPD Assessment Test) score. Patients and Methods: We extracted data from the multicenter Korea COPD Subgroup Study (KOCOSS) cohort, for which patients recruited from among 47 medical centers in South Korea beginning in April 2012. Patients were classified according to three different definitions of CB: 1) classical definition; 2) SGRQ (using questions regarding cough and sputum); and 3) CAT score (comprising cough [CAT1] and sputum [CAT2] subscale scores). Results: A total of 2,694 patients were enrolled in this study. The proportions of CB were 10.8%, 35.8%, and 24.0% according to the classical, SGRQ, and CAT definitions, respectively. The three definitions yielded consistently significant differences between CB and non-CB patients in modified Medical Research Council dyspnea scale CAT score, SGRQ score, number of moderate to severe exacerbations per year and forced expiratory volume in 1 second. By three definitions, CB consistently predicted future risk of exacerbation. The kappa coefficient of agreement between the classical definition and SGRQ definition was 0.29, that of the classical definition and CAT definition was 0.32, and that of the SGRQ definition and CAT definition was 0.44 (figure). Conclusion: Patients with CB according to the new definitions based on SGRQ or CAT score showed similar clinical characteristics to those defined according to the classical definition. The new CB definitions may be used as alternatives to the classical definition.

Intermittent Fasting Attenuates Allergic Airway Disease in OVA Induced Obese Mice via Interplay Between Leptin and Innate Lymphoid Cells

( Ji Young Kang ),( Jung Hur ),( Jiwon Ryoo ),( Hyungjoon Cho ),( Jongmin Lee ),( Hwa Young Lee ),( Chin Kook Rhee ),( Seung Joon Kim ),( Seok Chan Kim ),( Sook Young Lee ),( Soon Seog Kwon ),( Young 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0

Purpose As obesity is a crucial risk factor in asthma, diet control such as fasting could be beneficial effect in the disease control. Recently innate lymphoid cells (ILCs) are known early pivotal player in asthma pathogenesis and obesity. We investigate to the effect of intermittent fasting and the role of ILCs as its mechanism in an obese allergic asthma model. Methods In 3-week- old C57BL/6, obese asthma was induced by high-fat diet (HFD) for 8 weeks with subsequent ovalbumin (OVA) administration for 8 weeks. Intermittent fasting (IF) group skipped a meal for 24 hours from 12 weeks to 16 weeks. Typical parameters of asthma were measured. Adipokines such as leptin/adiponectin and ILCs profiles were examined. Results In the HFD-OVA group, pathognomic findings of asthma including AHR, airway inflammation, submucosal gland hyperplasia, and collagen deposition were observed prominently. Leptin level in serum increased whereas adiponectin decreased. Expression of ILC2 and ILC3 in the lung tissue were elevated. In the HFD-OVA-IF group, AHR decreased significantly and pathologic findings in asthma by PAS stain and hydroxyproline assay ameliorated. After IF, the concentration of leptin diminished, but that of adiponectin did not change remarkably. Notably, airway inflammation and type 1/2 cytokines increased without any statistical significance. The infiltration of ILC2 and ILC3 in the lung reduced in the HFD-OVA-IF group Conclusion This study reveals that intermittent fasting attenuates AHR and pathologic features such as submucosal gland hyperplasia and collagen deposition, not airway inflammation in an obese asthma model via a modulation of leptin and ILCs.

積符號의 短縮 復號法에 關한 硏究

金永晙(YOUNG JOON KIM),李晩榮(MAN YOUNG RHEE) 대한전자공학회 1985 대한전자공학회 학술대회 Vol.1985 No.6

울산지역에서 주폐경기 ( Perimenopause ) 여성의 골다공증 유병률과 연관인자

김영일(Young Il Kim),박재후(Jae Hoo Park),이종수(Jong Soo Lee),김진우(Jin Woo Kim),양승오(Seoung Oh Yang),전대준(Dae Joon Jeon),김문찬(Mun Chan Kim),정태흠(Tae Heum Jeong),이윤구(Yoon Gu Lee),이병두(Byoung Doo Rhee) 대한내과학회 2002 대한내과학회지 Vol.62 No.1

Background: Osteoporotic fractures pose a major public problem, not only in Western populations but also of increasing significance in Asian populations. However, most previous studies for the prevalence of osteoporosis in Korea were carried out from the data of hospitalized or out-patient based subjects, hospital staff, healthy volunteers and so forth. The purpose of present study was to evaluate the normative data of bone mineral density (BMD) in Korean young and perimenopausal women and to determine the prevalence and risk factors of low bone mass, including osteoporosis and osteopenia in a community- based population. Methods: In 1999, a baseline survey was carried out in the Dong-gu of Ulsan city, a urban area but located in seashore of southeastern Korea. The selected community sample of 3,822 inhabit ants (all were women aged 49-54 years) were chosen. We studied 1,629 women among them and 127 healthy women aged 20-35 years. BMD of lumbar spine and femur was measured using dual-energy X-ray absorptiometry. For analysis of risk fact ors, those were excluded who was the current or recent user of estrogen and who had osteoporosis related disease or unknown menopause due to previous hysterectomy. Thus, 1,020 subjects were analysed for the association of low BMD and risk factors. Significant determinants of BMD were investigated using univariate and multiple regression analysis. Results: When our young normal data were used, 9.8% for the lumbar spine and 1.4% for the femoral neck of our study population (n=1,629) were classified as osteoporosis. Among them, the menopausal status of above 1,020 subjects had independent association with low BMD (T-score<-1.0) of both lumbar spine (odds ratio=4.71 in postmenopause, p <0.001) and femoral neck (odds ratio=2.86 in postmenopause, p <0.001). In premenopausal women (n=507), weight and age of menarche were associated independently with low bone mass, including osteoporosis and osteopenia of both lumbar spine and femoral neck. In postmenopausal women (n=513), weight and duration since menopause were associated independently with low bone mass of both lumbar spine and femoral neck. Also, daily amount of calcium intake had significant association (regression coefficient =0.047; p <0.05) independently with BMD of femoral neck. Conclusion: The result s of this population-based study suggest that it is import ant to prevent bone loss and screen the BMD in women aged 49-54 years with menopause, low body weight, late menarche, prolonged duration since menopause, low calcium intake.(Korean J Med 62:11- 24, 2002)

Effect of Ageing on Pulmonary Inflammation in a Murine Model of Acute Asthma

( Ji Young Kang ),( Chin Kook Rhee ),( Chan Kwon Park ),( Seong Joon Kim ),( Seok Chan Kim ),( Soon Seog Kwon ),( Young Kyoon Kim ),( Hwa Sik Moon ),( Jeong Sup Song ),( Sung Hak Park ),( Sook Young L 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.-

Adipose tissue-derived mesenchymal stem cells cultured at high cell density express brain-derived neurotrophic factor and exert neuroprotective effects in a 6-hydroxydopamine rat model of Parkinson’s disease

Park, Joon Beom,Lee, Jin Suk,Cho, Byung Pil,Rhee, Ki-Jong,Baik, Soon Koo,Kim, Jiye,Kang, Seong Joon,Park, Dong-Joon,Oh, Ji-Eun,Shin, Ha Cheol,Kim, Yong Man,Kim, Hyun Soo,Bae, Keum Seok,Eom, Young Woo Springer-Verlag 2015 Genes & Genomics Vol.37 No.2

The Korean Academy of Tuberculosis and Respiratory Diseases : Slide Session ; OS-096 : Critical Care ; Utility of High-Flow Nasal Cannula Oxygen Therapy for Acute Respiratory Failure in Patients with Hematologic Malignancies: A Retrospective Study in a Si

( Hwa Young Lee ),( Hea Yon Lee ),( Ji Young Kang ),( Seung Joon Kim ),( Young Kyoon Kim ),( Sook Young Lee ),( Seok Chan Kim ),( Chin Kook Rhee ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Recently, it is suggested that high ow nasal cannmula (HFNC) therapy may provide an effective ventilation and tolerability in patients with moderate to severe acute respiratory failure. This study investigated utility of HFNC for acute respiratory failure in adult patients with hematologic malignancies. Methods: We identifi ed 52 acute respiratory failure patients with hematologic malignancies who received HFNC therapy between March 2012 and Jun 2014 in Seoul St. Mary`s hospital. Retrospective chart review was done to identify useful prognostic factors for succesful treatment. Results: Of the 52 patients, 15 (28.8 %) patients had successfully recovered and 30 (57.7 %) changed to invasive ventilation due to the HFNC treatment failure. The most common hematologic malignacies were acute leukemia (53.8 %), myelodysplastic syndrome (11.5 %) and lymphoma (11.5 %). The etiologies of acute respiratory failure were bacterial pneumonia (33, 61.5 %), pneumocystitis carinii pneumonia (7, 13.5 %), pulmonary edema (5, 9.6 %) and bronchiolitis obliterans organizing pneumonia (4, 7.7 %). The overall mortality was 63 %. HFNC treatment success was signifi cantly different factor between the survivors and nonsurvivors. In univariate analysis between HFNC success and failure groups, infectious pneumonia was associated with HFNC treatment failure with shorter duration of therapy (49.5 ± 9.85 hours vs. 77.7 ± 15.92 hours, P = 0.044) and longer ICU stay (13.7 ± 1.57 days vs. 7.6 ± 1.52 days, P = 0.039).Conclusion : HFNC therapy could provide successful oxygenation in acute respiratory failure patients with hematologic malignancies. Among the patients diagnosed acute respiratory failure, patients with infectious pneumonia would rather be monitored carefully to determine whether to continue HFNC therapy or convert to invasive ventilation.

TP-4 : Thematic Poster ; Effect of Low Dose Azithromycin on Airway Inflammation in a Murine Model of Acute Asthma

( Ji Young Kang ),( Hye Seon Kang ),( Hye Yon Lee ),( Chin Kook Rhee ),( Seong Joon Kim ),( Seok Chan Kim ),( Soon Seog Kwon ),( Young Kyoon Kim ),( Sook Young Lee ) 대한결핵 및 호흡기학회 2013 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.116 No.0

Background and Objectives: Recent research demonstrate that administration with low dose macrolide has an effect on chronic inflammatory airway diseases such as chronic obstructive pulmonary disease through anti-inflammatory action. However, the influence of the drug on asthma is not clearly verified. The aim of this study was to investigate whether low dose azithromycin, a kind of macrolide, affects on allergic airway inflammation in a murine model of acute asthma. Methods: 6 weeks old-female BALB/c mice were sensitized and challenged with ovalbumin. Low dose azithromycin (75 mg/kg) was administered subcutaneously daily 30 min before the challenge for 1 week. Six mice were used in each group (control vs OVA vs Azithromycin). Total and differential cell counts in bronchoalveolar lavage fluid (BALF) were measured and Th2 cytokine profiles in BALF were analyzed by ELISA. Interleukin (IL)-17, neutrophils elastase, IL-8 were assessed. Results: The BALF counts in total cell and eosinophils decreased significantly after the azithromycin administration. Th2 cytokines such as IL-4, IL-5, and IL-13 showed lower concentrations in azithromycin group than in OVA group. The levels of IL-17, netrophil elastase, and IL-8, which are known as parameters are related with the action of azithromycin, lessened by the treatment with azithromycin. Conclusion: This study suggests that low dose azithromycin has some effects on airway inflammatory responses in acute asthma of murine model.Background and Objectives: Recent research demonstrate that administration with low dose macrolide has an effect on chronic inflammatory airway diseases such as chronic obstructive pulmonary disease through anti-inflammatory action. However, the influence of the drug on asthma is not clearly verified. The aim of this study was to investigate whether low dose azithromycin, a kind of macrolide, affects on allergic airway inflammation in a murine model of acute asthma. Methods: 6 weeks old-female BALB/c mice were sensitized and challenged with ovalbumin. Low dose azithromycin (75 mg/kg) was administered subcutaneously daily 30 min before the challenge for 1 week. Six mice were used in each group (control vs OVA vs Azithromycin). Total and differential cell counts in bronchoalveolar lavage fluid (BALF) were measured and Th2 cytokine profiles in BALF were analyzed by ELISA. Interleukin (IL)-17, neutrophils elastase, IL-8 were assessed. Results: The BALF counts in total cell and eosinophils decreased significantly after the azithromycin administration. Th2 cytokines such as IL-4, IL-5, and IL-13 showed lower concentrations in azithromycin group than in OVA group. The levels of IL-17, netrophil elastase, and IL-8, which are known as parameters are related with the action of azithromycin, lessened by the treatment with azithromycin. Conclusion: This study suggests that low dose azithromycin has some effects on airway inflammatory responses in acute asthma of murine model.