The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Yang Qianqian,Yan Ding,Zou Chaoying,Xue Qian,Lin Shuhui,Huang Qingtian,Li Xiaofen,Tang Daolin,Chen Xin,Liu Jinbao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Triple-negative breast cancer (TNBC) is a heterogeneous malignancy in women. It is associated with poor prognosis, aggressive malignant behavior, and limited treatment options. In the ubiquitin‒proteasome system (UPS), deubiquitinases (DUBs) are potential therapeutic targets for various tumors. In this study, by performing unbiased siRNA screening, we identified STAMBP, a JAMM metalloprotease in the DUB family, as a driver of human TNBC tumor growth. Functionally, the knockdown of STAMBP inhibited the proliferation, migration, and invasion of multiple TNBC cell lines. Immunoprecipitation–mass spectrometry combined with functional and morphological analysis verified the interaction between STAMBP and the actin-binding protein RAI14. Mechanistically, STAMBP stabilized the RAI14 protein by suppressing the K48-linked ubiquitination of RAI14 and thus prevented its proteasomal degradation. Therefore, knocking down STAMBP resulted in the reduction in RAI14 protein levels and suppression of tumor growth in vitro and in vivo. Importantly, high levels of STAMBP were correlated with poor prognosis in TNBC patients. In summary, we reveal a previously unrecognized DUB pathway that promotes TNBC progression and provides a rationale for potential therapeutic interventions for the treatment of TNBC.

Fluorescent Microarrays of <i>in Situ</i> Crystallized Perovskite Nanocomposites Fabricated for Patterned Applications by Using Inkjet Printing

Liu, Yang,Li, Fushan,Qiu, Lichun,Yang, Kaiyu,Li, Qianqian,Zheng, Xin,Hu, Hailong,Guo, Tailiang,Wu, Chaoxing,Kim, Tae Whan American Chemical Society 2019 ACS NANO Vol.13 No.2

<P>Perovskite materials have exhibited promising potential for universal applications including backlighting, color conversion, and anticounterfeiting labels fabricated using solution processes. However, owing to the tendency of those materials to have uncontrollable morphologies and to form large crystals, they cannot be utilized in discontinuous microminiaturization, which is crucial for practical optoelectronic applications. In this research, combining the effects of adding polyvinylpyrrolidone (PVP), precisely controlling the inkjet printing technique, and using a postprocessing procedure, we were able to fabricate <I>in situ</I> crystallized perovskite-PVP nanocomposite microarrays with perfect morphologies. The viscosity of the perovskite precursor increased with the addition of PVP, eliminating the outward capillary flow that induces the coffee-ring effect. In addition, because of the presence of metallic bonds with the C═O groups in PVP and the spatial confinement of such a polymer, we were able to fabricate regulated CsPbBr<SUB>3</SUB> nanocrystals capped with PVP and with a uniform size distribution. The as-printed patterns showed excellent homogeneity on a macroscale and high reproducibility on a microscale; furthermore, those patterns were invisible in the ambient environment, compatible with flexible substrates, and cost-efficient to produce, indicating that this technique holds promising potential for applications such as anticounterfeiting labels.</P> [FIG OMISSION]</BR>

Functional analysis of the HD-Zip I gene ZmHDZ1 in ABA-mediated salt tolerance in rice

Qianqian Wang,Kangyong Zha,Wenbo Chai, Yu Wang,Bin Liu,Haiyang Jiang,Beijiu Cheng,Yang Zhao 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

The homeodomain-leucine zipper I (HD-Zip I) transcription factors play crucial roles in the regulation of abiotic stress responses, whereas, up to now, most of them are still functionally unknown. In present study, we identified a maize HD-Zip I gene, designing as ZmHDZ1, and investigated its potential roles in salt stress response. The qRT-PCR results showed that expression of ZmHDZ1 was induced by ABA and salt stress. Transactivation assay and transient expression analyses indicated that ZmHDZ1 protein was located in the nucleus in tobacco leaf cells and had the transactivation activity in yeast. Over-expression of ZmHDZ1 in rice reduced tolerance to salt stress, thus, led to greater accumulation of malondialdehyde (MDA) as well as higher level of relative electrolyte leakage (REL) compared to WT plants. In addition, the transgenic seedlings also increased sensitivity to exogenous ABA. Furthermore, the expression levels of four salt responsive genes were different between transgenic and WT rice under normal or NaCl treatment. These results suggested that ZmHDZ1 function as a negative regulator in response to salt stress through the ABAmediated signal transduction pathways.

Functional roles and mechanisms of ginsenosides from Panax ginseng in atherosclerosis

Qianqian Xue,Ningning He,Zhibin Wang,Xiuxiu Fu,Lynn Htet Htet Aung,Yan Liu,Min Li,Jae Youl Cho,Yanyan Yang,Tao Yu 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.1

Atherosclerosis (AS) is a leading cause of cardiovascular diseases (CVDs) and it results in a high rate of death worldwide, with an increased prevalence with age despite advances in lifestyle management and drug therapy. Atherosclerosis is a chronic progressive inflammatory process, and it mainly presents with lipid accumulation, foam cell proliferation, inflammatory response, atherosclerotic plaque formation and rupture, thrombosis, and vascular calcification. Therefore, there is a great need for reliable therapeutic drugs or remedies to cure or alleviate atherosclerosis and reduce the societal burden. Ginsenosides are natural steroid glycosides and triterpene saponins obtained mainly from the plant ginseng. Several recent studies have reported that ginsenosides have a variety of pharmacological activities against several diseases including inflammation, cancer and cardiovascular diseases. This review focuses on describing the different pharmacological functions and underlying mechanisms of various active ginsenosides (Rb1,-Rd, -F, -Rg1, -Rg2, and -Rg3, and compound K) for atherosclerosis, which could provide useful insights for developing novel and effective anti-cardiovascular drugs.

Protective effect and mechanism of ginsenoside Rg2 on atherosclerosis

Qianqian Xue,Tao Yu,Zhibin Wang,Xiuxiu Fu,Xiaoxin Li,Lu Zou,Min Li,Jae Youl Cho,Yanyan Yang The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Background: Ginsenoside Rg2 (Rg2) has a variety of pharmacological activities and provides benefits during inflammation, cancer, and other diseases. However, there are no reports about the relationship between Rg2 and atherosclerosis. Methods: We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to detect the cell viability of Rg2 in vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). The expression of inflammatory factors in HUVECs and the expression of phenotypic transformation-related marker in VSMCs were detected at mRNA levels. Western blot method was used to detect the expression of inflammation pathways and the expression of phenotypic transformation at the protein levels. The rat carotid balloon injury model was performed to explore the effect of Rg2 on inflammation and phenotypic transformation in vivo. Results: Rg2 decreased the expression of inflammatory factors induced by lipopolysaccharide in HUVECs-without affecting cell viability. These events depend on the blocking regulation of NF-κB and p-ERK signaling pathway. In VSMCs, Rg2 can inhibit the proliferation, migration, and phenotypic transformation of VSMCs induced by platelet derived growth factor-BB (PDGF-BB)-which may contribute to its anti-atherosclerotic role. In rats with carotid balloon injury, Rg2 can reduce intimal proliferation after injury, regulate the inflammatory pathway to reduce inflammatory response, and also suppress the phenotypic transformation of VSMCs. Conclusion: These results suggest that Rg2 can exert its anti-atherosclerotic effect at the cellular level and animal level, which provides a more sufficient basis for ginseng as a functional dietary regulator.

Layout optimization of box girder with RBF-NNM-APSO algorithm

Junle Yang,Yixiao Qin,Qianqian Jiao 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11

The layout optimization problem of complex box girder structure is solved with a new method RBF-NNM-APSO formed with the digital neural network model (NNM) of radial basis function (RBF) and adaptive particle swarm optimization (APSO) algorithm in this paper. The optimized surrogate model is proposed and applied to the configuration optimization of heavy-duty box girder of casting crane for improving the mechanical properties of the optimized object and expediting proceedings. First, the parametric command flow finite element numerical model of box girder is established. The RBF neural network is trained by constructing a mixed orthogonal experimental table of parameters, and the relationship between the design variables and the maximum stress and deformation is established. Subsequently, the trained RBF neural network design scheme is optimized by APSO algorithm. Finally, on the premise of not increasing the total mass, a new layout form of box girder is obtained.

Epigenetically Modified Bone Marrow Stromal Cells in Silk Scaffolds Promote Craniofacial Bone Repair and Wound Healing

Han, Qianqian,Yang, Pishan,Wu, Yuwei,Meng, Shu,Sui, Lei,Zhang, Lan,Yu, Liming,Tang, Yin,Jiang, Hua,Xuan, Dongying,Kaplan, David L.,Kim, Sung Hoon,Tu, Qisheng,Chen, Jake Mary Ann Liebert 2015 Tissue engineering. Part A Vol.21 No.15

<P>Epigenetic regulation of gene expression is a central mechanism that governs cell stemness, determination, commitment, and differentiation. It has been recently found that PHF8, a major H4K20/H3K9 demethylase, plays a critical role in craniofacial and bone development. In this study, we hypothesize that PHF8 promotes osteoblastogenesis by epigenetically regulating the expression of a nuclear matrix protein, special AT-rich sequence-binding protein 2 (SATB2) that plays pivotal roles in skeletal patterning and osteoblast differentiation. Our results showed that expression levels of PHF8 and SATB2 in preosteoblasts and bone marrow stromal cells (BMSCs) increased simultaneously during osteogenic induction. Overexpressing PHF8 in these cells upregulated the expression of SATB2, Runx2, osterix, and bone matrix proteins. Conversely, knockdown of PHF8 reduced the expression of these genes. Furthermore, ChIP assays confirmed that PHF8 specifically bound to the transcription start site (TSS) of the SATB2 promoter, and the expression of H3K9me1 at the TSS region of SATB2 decreased in PHF8 overexpressed group. Implantation of the BMSCs overexpressing PHF8 with silk protein scaffolds promoted bone regeneration in critical-sized defects in mouse calvaria. Taken together, our results demonstrated that PHF8 epigenetically modulates SATB2 activity, triggering BMSCs osteogenic differentiation and facilitating bone formation and regeneration in biodegradable silk scaffolds.</P>

Enhancing the adsorption function of F⁻ by iron and zirconium doped zeolite: Characterization and parameter optimization

Bo Yang,Chaomin Jia,Guirong Sun,Bingxu Quan,Chunhui Zhang,Qianqian Huo,Peidong Su 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2

This study presented an original study on the F⁻ removal by Fe and Zr doped Gismondine-dominated Zeolite. Various modified zeolites are prepared by systematically adjusting the synthesis variables, namely pH, mass ratio of zeolite to modify agent (m<SUB>Zeolite:m(Fe+Zr)</SUB>), mass ratio of Fe to Zr (Fe:Zr) to investigate their effects on the F⁻ adsorption. The performance of prepared Fe-Zr-Zeolite on F⁻ removal was examined through both statistic adsorption and dynamic adsorption. Results indicated that when the modified pH was 7, the Fe:Zr ratio was 1:1 and m<SUB>Zeolite:m(Fe+Zr)</SUB> was 1:2, the modified natural zeolites which was named Fe-Zr-Zeolite showed the best removal efficiency on F⁻. Fe-Zr-Zeolite could remove over 80% when the initial concentration was less than 20 mg/L and F⁻ adsorption followed pseudo-second-order adsorption kinetic and Langmuir adsorption isotherm, indicating that F⁻ adsorption by Fe-Zr-Zeolite was chemically dominated. Besides, Fe-Zr-Zeolite had better removal efficiency of F⁻ under acidic conditions than that under alkaline conditions, and the competing anions, typically, Cl-, NO₃⁻ and SO₄<SUP>2-</SUP> had negligible effect on F⁻ removal by Fe-Zr-Zeolite. The dynamic adsorption test demonstrated that in order to maintain the high removal efficiency of F⁻, the filling thickness of Fe-Zr-Zeolite should be at least 30 ㎝ and the small the flow rate is, the higher the F⁻ removal efficiency will be.

The design of large packaging production line system based on Embedded Technology

Huan Yang,Qianqian Zhang,Chenghao Yuan,Hancheng zhao,Yanjun Xiao 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.8

CT 와 IVUS 데이터를 이용한 혈관 모델링 기술

장치엔치엔(Qianqian Zhang),양상욱(Sangwook Yang),최영(Young Choi) (사)한국CDE학회 2011 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2011 No.1

In achieving 3D model of blood vessel from computed tomography(CT), it is not easy to separate vascular endothelium and vascular wall since the portion of the blood vessel in a CT volume data is relatively too small and has not sufficient resolution nor distinctive voxel intensity. In case of using vascular contrast agent, detection of the vascular endothelium is feasible but detection of vascular wall is still difficult. This paper proposes a 3D reconstruction process of blood vessel from CT data with intravascular ultrasound(IVUS) data. The shape of vascular endothelium is achieved from CT data by segmentation and then converted to polygonal data with iso-surface extraction. A representative shape of the polygonal data is approximated into a B-spline curve. If a vascular wall shape from an IVUS image is appropriately positioned on the curve at the location of the IVUS image acquisition, the cross-section shape of vascular wall on the location is defined. The 3D surface model of vascular endothelium and vascular walls is constructed from the series of cross-section contours.