Chemical Vapor Deposition Growth of Graphene Domains Across the Cu Grain Boundaries

Yang Wang,Yu Cheng,Yunlu Wang,Shuai Zhang,Chen Xu,Xuewei Zhang,Miao Wang,Yang Xia,Qunyang Li,Pei Zhao,Hongtao Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.08

Many aspects in the chemical vapor deposition (CVD) growth of graphene remain unclear such as its behavior near the catalyst grain boundaries. Here we investigate the CVD growth mechanism of graphene across the Cu grain boundaries using unidirectional aligned graphene domains, which simplifies the analysis of both graphene and Cu to a large extent. We found that for a graphene domain grown across the Cu grain boundary, the domain orientation is determined by the Cu grain where the domain nucleation center is located, and the Cu grain boundary will not change the growth behavior for this graphene domain. This growth mechanism is consistent with the Custep-attached nucleation and edge-attachment-limited growth mechanism for H-terminated graphene domains and will provide more guidance for the synthesis of high-quality graphene with less domain boundaries.

Changes in the physicochemical properties and in vitro protein digestibility of peanut hulls treated via mechanical activation

Miao-miao Feng,Ya-fen Wang,Xuan Cai,Hong-cai Zhang,Jian-xiong Xu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.6

In this study, peanut hulls powder (PHP) wastreated via mechanical activation (MA) and divided intothree groups (control, PHP150 and PHP250). Physicochemicalproperties including mean particle size distribution(MPSD), powder properties, solubility and in vitro proteindigestibility of PHP were then investigated. The resultsshowed that MA could decrease the particle size of PHP bydestroying its crystal structure, resulting in an increase ofamorphization and a decrease of crystallinity and crystallinesize. The results of in vitro protein digestibility and crudefi ber contents showed that MA increased the protein digestibilityof PHP by 43.32% and 74.70% ( P < 0.05), while crudefi ber content was decreased by 0.42% and 26.65% ( P < 0.05). These fi ndings indicated a large application potential of MAin PHP treatment.

Gemcitabine-based Concurrent Chemoradiotherapy Versus Chemotherapy Alone in Patients with Locally Advanced Pancreatic Cancer

Wang, Bu-Hai,Cao, Wen-Miao,Yu, Jie,Wang, Xiao-Lei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To explore improved treatment by retrospectively comparing survival time of gemcitabine-based concurrent chemoradiotherapy (GemRT) versus chemotherapy (Gem) alone in patients with locally advanced pancreatic cancer (LAPC). Methods: From January 2005 to June 2010, 56 patients with LAPC from Subei People's Hospital were treated either with Gem (n=21) or GemRT (n=35). Gem consisted of 4-6 cycles gemcitabine alone (1000 mg/m2 on Days 1, 8, 15, 28-day a cycle). GemRT consisted of 50.4Gy/28F radiotherapy with concurrent 2 cycles of gemcitabine (1000 $mg/m^2$ on days of radiation 1, 8, 15, 21-day a cycle). Radiation was delivered to the gross tumor volume plus 1-1.5 cm by use of a three-dimensional conformal technique. The follow-up time was calculated from the time of diagnosis to the date of death or last contact. Kaplan-Meier methodology wes used to evaluate survival. Results: Patient characteristics were not significantly different between treatment groups. The disease control rate and the objective response rate of GemRT versus Gem was 97.1% vs 71.4%, 74.3% vs 38.1%. The overall survival (OS) was significantly better for GemRT compared to Gem (median 13 months versus 8 months; 51.4% versus 14.3% at 1 year, respectively). Conclusion: Radiation therapy at 50.4Gy with 2 concurrent cycles of gemcitabine results in favorable rates of OS. Concurrent chemoradiotherapy should be the first choice for patients with LAPC.

Organizational Commitment of Five Working Groups of MNCs

Miao Wang,Jong-Wook Kwon,Jun-Jian Li 강원대학교 경영경제연구소 2023 Asia-Pacific Journal of Business Vol.14 No.1

Purpose - The purpose of this study is to confirm that five different working positions show different Organizational Commitment (OC) and factors influencing OC levels in the Chinese MNCs. Design/methodology/approach - The study collected data of 326 employees in different positions in Chinese MNCs abroad and in domestic country. To test hypotheses, ANOVA analysis and linearity regression are used with SPSS 26 and AMOS 26 software on the collected data. Findings - Results shows that different working groups do not show significant difference of AC and CC level. However, there are significant difference of NC among the five groups in the Chinese MNCs. Employee's position and marital status are positively related to CC and NC. Tenure is positively related to AC. Research implications or Originality - Our research offers insight for further research direction on OC difference research. It provides implication for Chinese MNCs to consider more about strategies how to stimulate host country employee's normative commitment.

Bioactive Compounds / Food Microbiology : Improvement of Natamycin Production by Cholesterol Oxidase Overexpression in Streptomyces gilvosporeus

( Miao Wang ),( Shao Hua Wang ),( Gong Li Zong ),( Zhong Wen Hou ),( Fei Liu ),( D Joshua Liao ),( Xi Qiang Zhu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2

Natamycin is a widely used antifungal antibiotic. For natamycin biosynthesis, the gene pimE encodes cholesterol oxidase, which acts as a signalling protein. To confirm the positive effect of the gene pimE on natamycin biosynthesis, an additional copy of the gene pimE was inserted into the genome of Streptomyces gilvosporeus 712 under the control of the ermE* promoter (permE*) using intergeneric conjugation. Overexpression of the target protein engendered 72% and 81% increases in the natamycin production and cell productivity, respectively, compared with the control strain. Further improvement in the antibiotic production was achieved in a 1 L fermenter to 7.0 g/l, which was a 153% improvement after 120 h cultivation. Exconjugants highly expressing pimE and pimM were constructed to investigate the effects of both genes on the increase of natamycin production. However, the co-effect of pimE and pimM did not enhance the antibiotic production obviously, compared with the exconjugants highly expressing pimE only. These results suggest not only a new application of cholesterol oxidase but also a useful strategy to genetically engineer natamycin production.

Limiting the Number of Open Projects to Shorten the NPD Schedule

Miao-Ling Wang,Chun-I Yang,Sheng-Hung Chang 대한산업공학회 2011 Industrial Engineeering & Management Systems Vol.10 No.1

Many companies open multiple projects simultaneously due to market trends, which results in a crowding out effect because of limited resources. R&D engineers become overloaded and scheduling of product development is delayed resulting in timing misses and lost sales leads. The company in this case study (Company A), often opens up many projects simultaneously in order to respond to market needs quickly. The engineers are overloaded and, of course, the schedule is delayed. In order to identify problems, Company A began using Dr. Goldratt"s Thinking Processes (TP) during new product development (NPD). When the analysis phase of TP was completed, Company A"s core problem was identified as "the quantity of kick-off projects." Consequently, new rules and conditions and procedures were proposed for the opening, suspending, stopping, and closing of projects. Finally, the "Future Reality Tree" ensured that the proposed rules, conditions and procedures were set up as an available solution approved for practical application by executives. After a one-year trial run, the results showed that the Project Duration Rate was reduced by 53%, the Project Closed Rate was increased by 140% and the Project on Time Rate was increased from 10% to 68%. The above results give significant evidence of the benefits of the proposed methodology.

Effects of Dietary Supplementation with Hainanmycin on Protein Degradation and Populations of Ammonia-producing Bacteria In vitro

Wang, Z.B.,Xin, H.S.,Wang, M.J.,Li, Z.Y.,Qu, Y.L.,Miao, S.J.,Zhang, Y.G. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.5

An in vitro fermentation was conducted to determine the effects of hainanmycin on protein degradation and populations of ammonia-producing bacteria. The substrates (DM basis) for in vitro fermentation consisted of alfalfa hay (31.7%), Chinese wild rye grass hay (28.3%), ground corn grain (24.5%), soybean meal (15.5%) with a forage: concentrate of 60:40. Treatments were the control (no additive) and hainanmycin supplemented at 0.1 (H0.1), 1 (H1), 10 (H10), and 100 mg/kg (H100) of the substrates. After 24 h of fermentation, the highest addition level of hainanmycin decreased total VFA concentration and increased the final pH. The high addition level of hainanmycin (H1, H10, and H100) reduced (p<0.05) branched-chain VFA concentration, the molar proportion of acetate and butyrate, and ratio of acetate to propionate; and increased the molar proportion of propionate, except that for H1 the in molar proportion of acetate and isobutyrate was not changed (p>0.05). After 24 h of fermentation, H10 and H100 increased (p<0.05) concentrations of peptide nitrogen and AA nitrogen and proteinase activity, and decreased (p<0.05) $NH_3$-N concentration and deaminase activity compared with control. Peptidase activitives were not affected by hainanmycin. Hainanmycin supplementation only inhibited the growth of Butyrivibrio fibrisolvens, which is one of the species of low deaminative activity. Hainanmycin supplementation also decreased (p<0.05) relative population sizes of hyper-ammonia-producing species, except for H0.1 on Clostridium aminophilum. It was concluded that dietary supplementation with hainanmycin could improve ruminal fermentation and modify protein degradation by changing population size of ammonia-producing bacteria in vitro; and the addition level of 10 mg/kg appeared to achieve the best results.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Identification of reference genes for normalization of gene expression in Liposcelis entomophila (Psocoptera: Liposcelididae)

Miao Shiyuan,Yang Binbin,Wang Suisui,Wang Zhengyan,Lu Yujie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

Liposcelis entomophila is a nuisance pest that seriously threatens the safety of stored grains owing in part of pesticide resistance. To facilitate better control of L. entomophila, future studies need to focus on gene function characterization. This requires the identification of suitable reference genes (RGs) for reverse-transcription quantitative PCR (RT-qPCR), which is crucial for the normalization of target gene expression. Here, we evalu ated the expression stability of 12 candidate RGs in L. entomophila across six experimental conditions (devel opmental stage, population, body part, phosphine fumigation, hypoxia induction, and temperature treatment). Under developmental conditions, ACTB, HSP70, and RPL23 were the most stable RGs. Among the different populations and body parts, RPL23, EF1a, and ACTB were the most stable RGs. Comparison of gene expression among adults under different environmental conditions revealed that at least seven RGs were fit for normalizing gene expression data. Among them, RPL23, EF1a, and ACTB showed the best fit as RGs in RT-qPCR analysis. After validation, our results demonstrated that EF1a and ACTB, as well as their combination, were the ideal RGs for L. entomophila across various experimental conditions. This study provides a comprehensive RG selection sug gestion for gene expression studies of L. entomophila and will facilitate future functional genomic research on the development and tolerance of environmental factors in psocids.

How Do Firms in the Private Sector Perceive Constraints to Doing Business in China?

Miao Wang,Hong Zhuang 연세대학교 동서문제연구원 2019 Global economic review Vol.48 No.2

This paper studies perceptions of business constraints in China using rich firm-level data from the 2005 and 2012 World Bank Enterprise Surveys conducted jointly by the China National Bureau of Statics and the World Bank. In addition, we focus on the variations of perceptions across foreign and indigenous Chinese firms. Our results show that there exist significant differences in perceptions between foreign and domestic firms in China in the 2005 survey, but such differences are no long present in the 2012 survey. Our analysis also identifies several elements perceived as more severe constraints by both domestic and foreign firms.