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급성 구강감염부위에서 Staphylococcus lugunensis 의 특성
유용욱,이미성,차정단,김기경,신상희,문상은,김강주 원광대학교 생명공학연구소 1999 생명공학연구소보 Vol.6 No.1
Staphylococcus lugdunensis (S. lugdunensis) is a newly identified pathogenic species of coagulase negative staphylococci and an occasional but not rare cause of severe infections, such as infective endocarditis after dental extraction, bacteremia, osteomyelitis, peritonitis, and soft tissue infections. As antibiotic use increased, resistance rapidly developed. Some strains have plasmids related to antibiotic resistance. To characterize S. lugdunensis in acute oral infection, S. lugdunensis was isolated from the patients with acute oral abscess, osteomyelitis, and normal persons. Antibiotic resistance, in vitro cellular toxicity, in vivo virulence, δ-like hemolysin activity, and synergistic hemolysis on sheep blood agar plates were investigated. The dot blot analysis and Southern blot analysis of staphylococcal DNA was performed with δ-hemolysin gene probe of Staphylococcus aureus. (S. aureus) Staphylococcal DNA was cloned, nucleotide sequence was analysed, and homology was compared with other sequence in Gene Bank. S. aureus, S. lugdunensis, Staphylococcus cohnii (S. cohnii), and other coagulase negative staphylococci (CNS) were isolated from the patients with acute oral infection. The isolation ratio of S. lugdunensis in the patients with infection was higher than that of healthy persons, but the isolation ratio of S. aureus in the patients with infection was similar to that of healthy persons. S. lugdunensis from the patients with acute oral infection showed the resistance to penicillin, methicillin, cephalothin, and clindamycin. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had δ-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with S. aureus on sheep blood agar plates. In dot blot analysis, all strains of S. lugdunensis showed the positive reaction with the probe of δ-hemolysin gene in S. aureus, but a 7.3 kb HindⅢ fragment was observed in the DNA of S. lugdunensis that gave synergistic hemolysis in a Southern blot analysis. The molecular size of partially purified δ-hemolysin was about 50 kd. The cloned fragments from the chromosomal DNA of S. lugdunensis showed the partial homology with the insulin receptor-related and dopamine receptor of humans. These results suggest that S. lugdunensis might be an important pathogen in acute oral infection and show some homology with eukaryotes.
Mi-Dan Kang,Ga-Young Kim,Hyeon-Ji Jeon,Young-Hoon Jung 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Lipases are one of the widely used biocatalysts that can hydrolyze and synthesize esters in various reactions. In this study, a recombinant lipase derived from Lactobacillus rhamnosus IDCC 3201 was constructed. It was expressed in Escherichia coli BL21 (DE3) using pET-21a(+) vector and the produced His-tagged lipase was purified by Ni-NTA affinity chromatography. Molecular size of a lipase was showing approximately 24 kDa by SDS-PAGE. The purified lipase was successfully releasing fatty acids, showing olive oil as a on the chromogenic plate containing olive oil as a substrate. The maximum lipase activity (3438.5 U/mg) was observed at 60℃ and the activity was maintained until 90℃. In addition, at different pH of 7.0 to 11.0, the lipase activity was retained more than 70% activity. However, the addition of various cofactors did not show positive effects on enzyme activities. Also, among C10 to C18 chemicals, para-nitrophenyl laurate showed highest value of enzyme activities. In conclusion, the lipase from L. rhamnosus IDCC 3201 has high thermostable and pH-stable properties and can utilize a wide range of substrates which makes it a potential candidate for using in industry.
( Su Mi Kim ),( Choong Hak Park ),( Jin Wan Park ),( Yun Dan Kang ),( Jong Soo Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: The goal of this study is to determine the effect of sulforaphene with cisplatin combination treatment on ovarian cancer cell and to reveal potential mechanisms responsible for the effect. 방법: OVCAR-3 cells were treated with sulforaphene and cisplatin. After treatment, preliminary screening was done through 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) Assay. Reactive oxygen species (ROS) and mitochondrial membrane depolarization were studied. Furthermore, to explore the signaling mechanism of sulforaphene, western blot was conducted. Additionally, the expression of caspases-3, 8, 9, phosphoinositide 3-kinase (PI3K), and phosphatase and tensin homolog (PTEN) was analyzed. 결과: Sulforaphene enhances cisplatin efficacy through simultaneous activation of mitochondrial and external pathway of apoptosis as well as modulation of the expression of PI3K and PTEN. 결론: Combination treatment with the sulforaphene and cisplatin resulted in a tic increase in cytotoxic potency and apoptosis in human ovarian cancer NIH: OVCAR-3 cells. Our results suggest that sulforaphene as a chemo-enhancing adjuvant could improve the efficacy of cisplatin in treating ovarian cancer.
( Su Mi Kim ),( Jin Wan Park ),( Jong Soo Kim ),( Yun Dan Kang ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: Our purpose was to determine the prevalence and risk factors for maternal hypotension induced by regional anesthesia during elective cesarean section, and whether maternal hypotension has any impact on perinatal outcome. 방법: 440 mother-infant pairs were retrospectively analyzed between 2008 and 2013 at Dankook University hospital. Data collection included information on maternal blood pressure during the cesarean section that involved the use of regional anesthetics and any perinatal complications. 결과: A total of 90(20.5%) of the mothers experienced a decrease in their mean arterial blood pressure(MABP) by ≥ 30% compared with their preoperative MAPB and five mothers (1.1%) underwent a decrease in their MABP by ≥ 50% compared with their preoperative MAPB. The risk factors for hypotension included preoperative diagnosis of hypertension or a higher preoperative MABP. There were no significant differences in maternal diabetes, age, infant`s birth weight. There was no significant difference of the umbilical cord blood pH between the ones born to mothers whose MABP dropped ≥30% and the others born to mothers whose MABP dropped < 30%. There were also no differences in other selected infant`s parameters, such as meconium staining or Apgar score. 결론: Despite a very high prevalence of maternal hypotension after regional anesthesia during cesarean sections, term infants tend to tolerate this placental blood perfusion challenge in IV fluid resuscitation or anti-hypotensive such as ephedrine. Maternal hypotension occurring during elective cesarean section was not related to any short-term postnatal complication in term infants.
The Vascular Type Ehlers-Danlos Syndrome Diagnosed after Delivery
( Su-mi Kim ),( Yun-dan Kang ) 대한주산의학회 2017 Perinatology Vol.28 No.4
Vascular Ehlers-Danlos sydrome (vEDS) is a life-threatening autosomal dominant inherited disorder of connective tissue characterized by arterial aneurysm, dissection and rupture, bowel rupture, and rupture of the gravid uterus. vEDS results from mutation of COL3A1, which encodes the chains of type III collagen, a major protein in vessel walls and hollow organs. A 32-year-old primigravida had aneurysmal rupture in right posterior popliteal artery 9 days after induced vaginal delivery. The reason of labor induction was preterm premature rupture of membrane. She was diagnosed with vEDS by sequence analysis of COL3A1 mutation. A multidisciplinary team is required to provide tailored counseling and education about vEDS. Also, a long term follow up is need for individuals with vEDS.
( Yun Dan Kang ),( Soo Mi Kim ),( Ye Kyu Park ),( Jong Soo Kim ),( Jin Wan Park ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
In this study, collagen hydrogel has been evaluated as a stem cell carrier by a number of different criteria. First, we tested whether collagen hydrogel induced the secretion of neurotropic factors from UBC-MSCs. Second, by utilizing a 3-D co-culture with neuronal precursors, we tested the efficiency with which the neurotropic factors from UBC-MSCs were delivered to nearby neural precursors. We first tested whether the cell population that proliferated attached to the bottom of the plastic culture dish out of a mononuclear fraction obtained from the umbilical cord blood was enriched with mesenchymal stem cells by flow cytometric analysis. Next, we performed a RT-PCR analysis to examine neurotrophic factor and growth factor expression in the mesenchymal cell-enriched cell population of the umbilical cord blood. And we compared the 2-D culture of UBC-MSCs with the 3-D culture of UBC-MSCs in terms of neurotropic factor expression. We conducted co-culture experiments to examine whether UBS-MSCs in a collagen gel were capable of stimulating outgrowth and neuronal differentiation of human neuronal precursor cells (hNPCs). In comparison with 2-dimensional (2-D) liquid culture, 3-D culture of UCB-MSCs in a collagen-based hydrogel significantly enhanced the expression of various neutropic factors including neurotrophin-4, nerve growth factor, brain-derived neurotropic factor, and ciliary neurotropic factor as verified by the gene and protein analysis. In addition, a co-culture with human neuronal precursor cells significantly enhanced neurite outgrowth and neural differentiation of hNPCs as evidenced by enhanced expression of β-III tubulin and NeuN. Our results demonstrate that the collagen-based hydrogel is excellent at inducing the neurotrophic factor release from UBC-MSCs and providing a tropic support to hNPCs, suggesting the possibility that 3-D UBC-MSC culture in the collagen hydrogel could be a promising strategy for nerve regenerative medicines.