Contributed Mini Review : New paradigms on siRNA local application

( Meng Pan ),( Jin Wen Ni ),( Hui Ming He ),( Shan Gao ),( Xiao Hong Duan ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.3

Small interfering RNA (siRNA) functions through pairing with specific mRNA sequences and results in the mRNA`s degradation. It is a potential therapeutic approach for many diseases caused by altered gene expression. The delivery of siRNA is still a major problem due to its rapid degradation in the circulation. Various strategies have been proposed to help with the cellular uptake of siRNA and short or small hairpin RNA (shRNA). Here, we reviewed recently published data regarding local applications of siRNA. Compared with systemic delivery methods, local delivery of siRNA/shRNA has many advantages, such as targeting the specific tissues or organs, mimicking a gene knockout effect, or developing certain diseases models. The eye, brain, and tumor tissues are ‘hot’ target tissues/ organs for local siRNA delivery. The siRNA can be delivered locally, in naked form, with chemical modifications, or in formulations with viral or non-viral vectors, such as liposomes and nanoparticles. This review provides a comprehensive overview of RNAi local administration and potential future applications in clinical treatment. [BMB Reports 2015; 48(3): 147-152]

Hermite variational implicit surface reconstruction

Pan, R.,Meng, X.,Whangbo, T. SPRINGER SCIENCE + BUSINESS MEDIA 2009 Science in China. Series F, Information sciences Vol.52 No.2

Research of Lower Limb Rehabilitation Training Gait

Meng Zhang,Xiao li Wang,Pan Su,Yang Yudan 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.12

Gait analysis is an important part of the lower limb disorder walking rehabilitation training, based on the research of the walking gait, helpful for recovery equipment control system design has important significance. This article mainly through 3 d motion capture system, the movement of human lower limb joints character such as displacement, velocity, and the body center of gravity test analysis, and in view of the human lower limb joints, four degrees of freedom model is set up, and establish the corresponding mathematical model, and using the simulation software, to verify the rationality of structure, for the future of medical rehabilitation, and provide important data for the intelligent control of the organization.

METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner

Pan Xiaoping,Hong Xiaolv,Li Sumei,Meng Ping,Xiao Feng 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Breast cancer (BC) is the most prevalent malignant neoplasm among women and is the fifth most common cause of cancer-associated death worldwide. Acquired chemoresistance driven by genetic and epigenetic alterations is a significant clinical challenge in treating BC. However, the mechanism of BC cell resistance to adriamycin (ADR) remains to be elucidated. In this study, we identified the methyltransferase-like 3/microRNA-221-3p/homeodomain-interacting protein kinase 2/Che-1 (METTL3/miR-221-3p/HIPK2/Che-1) axis as a novel signaling event that may be responsible for resistance of BC cells to ADR. A dual-luciferase reporter gene assay was employed to test the presence of miR-221-3p binding sites in the 3′UTR of HIPK2. Drug resistance was evaluated by immunoblotting multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP). Cultured ADR-resistant MCF-7 cells were assayed for their half maximal inhibitory concentration (IC50) values and apoptosis using an MTT assay and Annexin V-FITC/PI-labeled flow cytometry, and the cells were then xenografted into nude mice. METTL3 knockdown was shown to reduce the expression of miR-221-3p by reducing pri-miR-221-3p m6A mRNA methylation, thereby reducing the IC50 value of ADR-resistant MCF-7 cells, reducing the expression of MDR1 and BCRP, and inducing apoptosis. Mechanistically, miR-221-3p was demonstrated to negatively regulate HIPK2 and upregulate its direct target Che-1, thus leading to enhanced drug resistance in ADR-resistant MCF-7 cells. In vitro results were reproduced in nude mice xenografted with ADR-resistant MCF-7 cells. Our work elucidates an epigenetic mechanism of acquired chemoresistance in BC, in support of the METTL3/miR-221-3p/HIPK2/Che-1 axis as a therapeutic target for the improvement of chemotherapy.

100W-class arcjet thrusters with ammonia propellant

Xian MENG,Wenxia PAN,Chengkang WU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Residual Risk and Its Risk Factors for Ischemic Stroke with Adherence to Guideline-Based Secondary Stroke Prevention

Yuesong Pan,Zixiao Li,Jiejie Li,Aoming Jin,Jinxi Lin,Jing Jing,Hao Li,Xia Meng,Yilong Wang,Yongjun Wang 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.1

Background and Purpose: Despite administration of evidence-based therapies, residual risk of stroke recurrence persists. This study aimed to evaluate the residual risk of recurrent stroke in acute ischemic stroke or transient ischemic attack (TIA) with adherence to guideline-based secondary stroke prevention and identify the risk factors of the residual risk. Methods: Patients with acute ischemic stroke or TIA within 7 hours were enrolled from 169 hospitals in Third China National Stroke Registry (CNSR-III) in China. Adherence to guideline-based secondary stroke prevention was defined as persistently receiving all of the five secondary prevention medications (antithrombotic, antidiabetic and antihypertensive agents, statin and anticoagulants) during hospitalization, at discharge, at 3, 6, and 12 months if eligible. The primary outcome was a new stroke at 12 months. Results: Among 9,022 included patients (median age 63.0 years and 31.7% female), 3,146 (34.9%) were identified as adherence to guideline-based secondary prevention. Of all, 864 (9.6%) patients had recurrent stroke at 12 months, and the residual risk in patients with adherence to guidelinebased secondary prevention was 8.3%. Compared with those without adherence, patients with adherence to guideline-based secondary prevention had lower rate of recurrent stroke (hazard ratio, 0.85; 95% confidence interval, 0.74 to 0.99; P=0.04) at 12 months. Female, history of stroke, interleukin- 6 ≥5.63 ng/L, and relevant intracranial artery stenosis were independent risk factors of the residual risk. Conclusions: There was still a substantial residual risk of 12-month recurrent stroke even in patients with persistent adherence to guideline-based secondary stroke prevention. Future research should focus on efforts to reduce the residual risk.

Design of Large-scale Boolean Networks Based on Prescribed Attractors

Jinfeng Pan,Jun-e Feng,Min Meng,Jianli Zhao 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.3

In this paper, the problem of designing large-scale Boolean networks with prescribed attractors is considered. The obtained Boolean networks satisfy the attractor constraints, as well as that the size of the basins of attraction (BOAs) of desired attractors is at its maximum. This is achieved by network aggregation, under which desired attractors and undesired attractors can be mapped to input-state cycles in the subnetworks. The input-state cycles corresponding to desired attractors must be contained in subnetworks, and for each undesired attractor at least one input-state cycle is not contained. An algorithm is proposed to maximize the size of the BOAs of desired attractors. Finally, a practical example is given to illustrate the effectiveness of the proposed methods.

Researchon the Development of One IP(intellectual property) Animation & Game in Chinese Market

Yang Pan,최철영,Zilu Meng 한국인터넷방송통신학회 2019 International Journal of Internet, Broadcasting an Vol.11 No.2

"No IP, No Content" has become a phenomenon in the content industry, high-quality IP has a strategic importance. It has become a Trinity in the movie-anime-game basic package, Competition is more intense. However, there is a tremendous amount of know-how hidden behind the use of IP to operate games well. We are worth exploring in detail. The research and analysis of digital IP market, the advantages and disadvantages will be the focus of our pragmatic research. As an emerging market in the digital industry, China has great research value. This paper analyzes the status quo of the current Chinese market by comparing the mature US and Japanese markets. For example, the research of American Disney Company analyzes the mature market structure of Japan. , compared with the excellent case of the Chinese market legend of qin.By studying the current situation of China's digital market, analyzing the interest trends of the customer base, discovering existing problems and improving the accuracy of the prediction and judgment of the Chinese digital market in the future.According to the survey, the IP heat is mainly concentrated in six categories of games, animation, TV, variety, movies, and novels. At present, the most popular IP of manufacturers have three major categories. This article will conduct research and analysis on digital IP, and analyze the market status of China, the United States, and Japan and the research on outstanding representative works in the market.

Influence of the roof lithological characteristics on rock burst: a case study in Tangshan colliery, China

Jienan, Pan,Zhaoping, Meng,Quanlin, Hou,Yiwen, Ju,Guofu, Li Techno-Press 2009 Geomechanics & engineering Vol.1 No.2

Many factors influence occurrences of rock burst in coal mines, such as mining methods, control methods of the coal roof, lithological characteristics of the roof and floor, tectonic stress, groundwater and so on. Among those factors, lithological characteristics in the roof are the intrinsic controlling factors that affect rock burst during coal mining. Tangshan colliery is one of the coal mines that have suffered seriously from rock bursts in China. In this paper, based on the investigating the lithological characteristics of coal roofs and occurrence of rock bursts in Tangshan colliery, a numerical method is used to study the influence of roof lithological characteristics on rock burst potential. The results show that the lithological characteristics in the roof have an important impact on the distributions of stresses and elastic strain energy in coal seams and their surrounding rocks. Occurrences of rock bursts in this colliery have a close correlation with the thick-bedded, medium- to fine-grained sandstones in the roof. Such strata can easily cause severe stress concentration and accumulate enough energy to trigger rock bursts in the working face during mining operations.

Preparation and photocatalytic activity of composite metal oxides derived from Salen-Cu(II) intercalated layered double hydroxides

Yue Meng,Shengjie Xia,Guoxiang Pan,Jilong Xue,Junhui Jiang,Zheming Ni 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.8

Copper(II) Schiff-base Complexes (Salen-Cu(II)) intercalated zinc-chromium layered double hydroxides (ZnSBCuCr-LDHs) were successfully prepared via coprecipitation. Then, a novel photocatalytic material Zn-Cu-Cr composite oxides (ZnSBCuCr-MO) was prepared after calcination at 500 oC with ZnSBCuCr-LDHs as precursor. The structure and properties of the materials were thoroughly characterized by powder X-ray diffraction (XRD), inductively coupled plasma atomic emission spectrometry (ICP-AES), ultraviolet visible diffuse reflectance spectroscopy (UV-Vis DRS), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The photocatalytic activities of the materials were investigated by photodegradation of a cationic dye Rhodamin B (RhB), which was hard to adsorb by LDHs. The effects of the photocatalyst dosage, the initial pH and temperature of RhB solution on the photocatalytic efficiency were discussed. The results showed that ZnSBCuCr-MO displayed higher photocatalytic activity for RhB than calcined ZnCr-LDHs (ZnCr-MO) and calcined ZnCuCr-LDHs (ZnCuCr-MO). Meanwhile, ZnSBCuCr- MO exhibited better stability and reusability. In addition, the possible photocatalytic mechanism and degradation pathway for RhB were studied through density functional theory (DFT) calculation of the RhB molecule, UV-vis absorption variation and LC-MS analyses of the RhB solution.