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Micropropagation of Atractylodes macrocephala Koidz. by Axillary Bud Culture
Mei Lan Jin(김미란),Yeon Bok Kim(김연복),Chinreddy Subramanyam Reddy(친레디 서부라만얌 레디),Jin Tae Jeong(정진태),Sang Hoon Lee(이상훈),Sung Cheol Koo(구성철),Mok Her(허목),Jae Ki Chang(장재기),Woo Moon Lee(이우문) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2
Mei Lan Jin,김청아,이상훈,허목,구성철,한종원,이우문 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Adenophora triphylla var japonica is a perennial herb that belongs to Campanulaceae. Radix Adenophorae is a dried rhizome of A. triphylla and same genus plant. It has contains chemicals such as cycloartenyl acetate, lupenone, β-sitosterol, taraxerone, octacosanoic acid, and praeruptorin A. Radix Adenophorae considered to be effective regulating humoral and cellular immunity, antimutation, restraining adenocarcinoma cell, strengthening cardiac function, allaying a fever, and easing pain and cough. In this study, we tried to establish a mass production system of A. triphylla which has high economic value as a medicinal herb by plant tissue culture in order to cultivate standard varieties. Methods and Results : In this study, A. triphylla internode was used as a explant and it was surface sterilized by 1% sodium hypochlorite for 5 minutes, consequently several times washed with ddH2O. Further it was placed in to MS medium including with axillary bud. The 1/2MS, B5, SH was used in this research. And the plant growth regulator of 0.1 - 2 ㎎/ℓ auxins (NAA, IBA) and cytokines (BA) were used respectively to achieve multiple shoots. The whole study was carried out in the department of Herbal crop research, Eumseong, RDA. Conclusion : In this study we obtained, 6.2 multi-shoots per an explant, and the shoot growth was also favorable in the presence of 1.0 ㎎/ℓ BA and 1.0 ㎎/ℓ IBA.