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Microplate법에 의한 수박 라이코핀 고함유 유전자원 선발
이우문,권민정,송이슬,김수,이희주,양은영,최학순,허윤찬,박동금,윤무경 한국육종학회 2014 한국육종학회지 Vol.46 No.1
Recent researches focused on breeding lycopene-rich watermelon varieties are active since lycopene content in watermelon is 60% higher than in tomato. We developed a new microplate method using a UV spectrophotometer to select genetic resources of watermelon containing high lycopene content. The microplate method showed more accurate analysis (about 97% positive correlation) than HPTLC, suggesting the microplate method is a reliable analytical technique for the rapid and simple selection of watermelon resources. Lycopene contents of 96 watermelon accessions using the new microplate method was a wide variation of 3.2-98.3 ㎍.g-1FW to assess, allowing selection of 15 high-lycopene watermelon accessions. Lycopene contents of the 15 watermelon accessions was 4.4∼9.6 fold higher than lycopene content of a commercial cultivar ‘Speedplus’. These results suggest that the watermelon accessions selected using a novel microplate method can be used for breeding of lycopene-rich cultivars. 수박은 토마토에 비하여 라이코핀 함량이 60% 높으며, 라이코핀 고함유 수박 품종개발에 관심이 집중되고 있다. 그러나 라이코핀 함량 분석을 위한 대량 분석 체계가 마련되지 않아 고함유 유전자원의 선발에 어려움이 있었다. 따라서 본 연구에서는 신속하고 간편한 lycopene 함량 분석기술의 개발과, 개발된 기술(microplate법)을 이용하여 라이코핀 고함유 유전자원을 선발하고자 하였다. 그 결과 UV spectrophotometer를 이용한 방법을 개선하여 Microplate법을 개발하였다. Microplate법은 HPTLC를 이용한 방법과 약 97%로 높은 정의 상관을 나타내어 분석의 정확성이 높은 방법임을 확인하였다. 따라서 Microplate법을 이용하여 수박 유전자원, 계통 및 96점을 대상으로 수박 과실의 라이코핀 함량을 분석한 결과 라이코핀 함량이 생체중 1 g 당 3.2∼98.3 μg·g-1의 넓은 변이를 보였다. 선발 유전자원은 시판 대비품종(스피드플러스; 10.3 μg·g-1 FW)에 비하여 4.4∼9.6배 높은 수준이었다. 선발된 자원들은 라이코핀 고함량 품종개발에 이용 될 수 있을 것으로 판단되었다.
禹文楨 嶺南大學校 工業技術硏究所 1991 연구보고 Vol.19 No.1
To investigate the enhancement in shear strength of reinforced earth an experimental study has been conducted using the modified shear box. The results of tests were evaluated in terms of the internal deformation and shear strength of soils, clay and sand. It is considered that there might be a similarity in the deformation between reinforced clay and sand, which is characterized as enlargement of deformation zone and restriction of relative movement of soils. It is suggest that the reinforcement plays a role in modifying the deformation of reinforced earth which results in the enhancement of shear strength, and that the reinforced earth may reasonably be understood by taking into account the altered deformation due to the inclusion of reinforcement.
외식소비자의 뷔페식당 이용특성이 고객만족도에 미치는 영향
우문호(Moon Ho Woo) 한국관광연구학회 2010 관광연구저널 Vol.24 No.2
This paper examined the effect of user`s characteristics of buffet restaurant on customer satisfaction as the moderator effect of service level. To examine this issue and generate empirical generations, the author conduct the relationship of the user`s characteristics of buffet restaurant, moderator effect of service level, customer satisfaction using a hierarchical linear model. The expectancy benefit of customer, external divergence, use convenience, and customer consideration were as proxy for the use characteristics of buffet restaurant. The product management, price management, place management, and promotion management were as proxy for the service level. The satisfaction for use characteristics of buffet restaurant and service level were as proxy for the customer satisfaction. Based on the analysis of one hundred eighty cases, the following results were found. First, the use characteristics of buffet restaurant, service level, and customer satisfaction will be drawn. Second, the use characteristics of buffet restaurant have a positively significant influence on customer satisfaction. Third, the interaction effects between the use characteristics of buffet restaurant and service level have a positively significant influence on customer satisfaction.
禹文楨 嶺南大學校 工業技術硏究所 1975 연구보고 Vol.3 No.1
To investigate the effect of effective overburden pressure on settlement, the observed and the predicted settlements have been considered with data based on 7 field records, using the modified N, or Ne instead of N values which are obtained by means of penetration test. The resulting chart is presented to facilitate design procedure.
于文生 조선대학교 동북아문제연구소 1995 동북아연구 Vol.1 No.1
近年來, 吉林省的對外貿易取得흔大成績, 特別是吉林省同周邊國家的邊境貿易表現尤爲突出, 直到 1994年上半年, 邊境貿易迅速發展, 然而, 從 1994年 下半年開始, 吉林省同周邊國家的邊境貿易出現滑坡, 而且下滑幅度흔大. 因此, 重新回顧邊境貿易的發展, 正確看待同周邊國家邊境貿易的下滑, 了解邊境貿易驟然下滑的原因, 對于吉林省今後고好同周邊國家邊境貿易, 積겁參與圖們江地區開發和東北亞區域經濟合作具有重要意義.