참당귀 뿌리 직경별 유효성분 함량 분석

구성철,허목,한종원,이상훈,김미란,홍충의,이이,이우문,장재기 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.05

Background : Angelica gigas Nakai (AGN) is a perennial plant found in East Asian countries and an crucial medicinal herb especially in Korea. It contains several chemicals like pyranocoumarins, which are major active components including decursin (D) and decursinol angelate (DA), essential oils and polyacetylenes. This study was carried out to determine the change in active components depending on the root diameter of AGN. Methods and Results : Several processing steps are involved to use AGN roots as medicine. The dried AGN roots are divided into body (B), thick root (TkR), medium root (MR) and thin root (TnR) according to their diameter before cutting into medicine. Among them, B and TkR are mainly used as medicine. The mean diameter of dried each root part (B, TkR, MR, TnR) were measured as 51.95, 7.05, 2.88, and 1.57 ㎜, respectively. The water content of each root part was slightly reduced depending on the size of the diameter (higher in B). In addition, the two active components, D and DA, were analyzed by high performance liquid chromatography. The change of both D and DA content showed a similar tendency. Both D and DA content were increased as the root diameter decreased (higher in TnR). Conclusion : This study showed that the thinner the root diameter, the higher the D and DA content in AGN roots, suggesting that the thinner root parts of AGN could be used as potential materials.

완두나물 재배에 적합한 유용자원 선발과 완두나물 적정 재배조건 설정

구성철,강범규,김현태,서정현,정광호,윤홍태,오인석,최만수 한국작물학회 2015 한국작물학회지 Vol.60 No.4

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25°C). However, whole length, hypocotyl length, and sprout yield were highly increased at 23°C compared to 20 and 25°C. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23°C than at 20 and 25°C, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts. 본 연구는 완두나물로 활용 가능한 유전자원을 선발하고, 그에 적합한 재배조건을 설정하고자 재배 온도 및 기간에따른 완두나물의 재배특성 및 항산화 활성을 분석하여 완두를이용한 채소용 나물 최적 재배 조건을 구명하고자 실시하였다. 1. 완두 유전자원 38점 중 완두 나물로 이용 가능한 자원으로 PI269803, PI343278, PI343283, PI343300와 PI343307 을 1차적으로 선발하였다. 2. 1차 선발된 완두자원을 대상으로 완두나물 재배 조건을설정하기 위하여 20°C, 23°C와 25°C에서 각각 7일간 재배하였다. 그 결과 전장과 배축장은 20°C와 25°C에 비해23°C에서 가장 높게 증가하였다. 3. 재배온도별 나물수율은 23°C에서 가장 높게 나타났으며, 유전자원 PI269803와 PI343300이 다른 자원에 비해 높은 나물수율을 나타내었다. 4. 재배온도별 완두나물의 조단백질 함량을 분석한 결과 온도에 따른 조단백질 변화는 나타나지 않았다. 5. 재배 온도별 완두나물의 폴리페놀 함량과 자유 라디칼소거능을 측정함으로써 항산화 특성을 조사하였다. 그결과 PI343278와 PI343283에서는 재배 온도가 증가함에 따라 폴리페놀 함량과 자유 라디칼 소거능이 감소하는 경향을 나타내었다. 그러나 PI269803와 PI343300에서는 20°C와 25°C에 비해 23°C에서 높은 폴리페놀 함량과 항산화 활성을 나타내었다. 6. 높은 나물수율과 항산화 특성을 보이는 PI269803과 PI343300은완두나물 이용에 적합한 자원으로 예상된다.

국내 더덕 속 식물의 형태학적 특성과 유효성분 함량 비교 분석

구성철,유진,장인복 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.5

Background: Among the Codonopsis plants, C. lanceolata and C. pilosula are cultivated for edi- ble and medicinal purposes. But basic agronomic data of C. ussuriensis are required for domestic cultivation. This study aimed to compare the agronomic characteristics of the three Codonopsis species for cultivation. Methods and Results: The three Codonopsis species were cultivated in the same field and their agronomic characteristics were compared. C. ussuriensis demonstrated similar morphological characteristics to C. pilosula, such as hairless stems and leaves, stem color, wingless seeds, and root weight. However, the shape and size of the leaf and the color of the corolla are intermediate between C. lanceolata and C. pilosula. However, all three species showed different root shapes and seed sizes and weights. In terms of phytochemical components, the main components of C. lanceo- lata and C. pilosula were tangshenoside I and lobetyolin, while C. ussuriensis was ussurienoside I. Moreover, all components demonstrated high content with increasing annual age. Conclusions: This study demonstrated different agronomic characteristics of C. ussuriensis com- pared to C. lanceolata and C. pilosula, and the results are valuable as basic data for cultivating C. ussuriensis.

대전광역시와 중국 지방정부 교류를 통해 본 지방 관계의 한계

구성철 대한중국학회 2018 대한중국학회 학술대회 발표집 Vol.2018 No.1

전분함량이 높은 콩 유전자원 선발

구성철,전명기,이영훈,김현영,강범규,고종민,백인열,윤홍태,최만수 한국육종학회 2014 한국육종학회지 Vol.46 No.1

본 연구는 고 전분 함유 유용 유전자원 선발 및 콩 우량품종 육성의 기초자료로 활용하고자 국내 콩 주요 품종 및 보존 유전자원의 전분함량과 변이 양상을 분석하였으며 그 결과는 다음과 같다. 1.총 2354점 국내 콩 주요 품종 및 보존 유전자원에 대한 요오드-전분 반응에 의한 착색 정도에 따라 1에서 4등급으로 분류를 하였으며, 대부분의 콩 유전자원이 2∼3등급으로 분류되었고, 가장 강한 착색 반응을 보인 4등급으로 126점의 유전자원이 선발되었다2.전분함량의 정량적 분석을 수행한 결과, 1, 2등급으로 분류된 유전자원 Daewang, Soheonje, Jyuiku #109는 각각 0.57, 0.45, 0.54%의 낮은 전분함량을 나타내었다. 반면, 4등급으로 분류된 유전자원은 2.81∼4.55%의 상대적으로 높은 전분함량을 보여 요오드-전분 반응법은 유전자원의 대량검정에 유용하게 이용할 수 있을 것으로 판단되었다.3.각 유전자원에서 전분함량의 차이가 다른 구성 성분의 함량에 영향을 미치는지를 알아보기 위해, 조단백질함량, 조지방함량, 수분함량을 각각 측정하였다. 전분함량의 변화는 조단백질 함량 변화에 영향을 미치며, 조지방과 수분함량의 변화에 영향이 없음을 확인하였다. Starch is the most important carbohydrate in the human and animal diets and it also has numerous industrial applications. Starch is widely used as a key material for processed foods such as bread, pancakes, cereals, noodles, pasta, porridge and tortilla. Increasing the starch content in food crops is one of the important targets for crop breeding. We used a starch-iodine test to identify varieties with high starch content in 2354 soybean germplasm collection and found a total of 126 germplasm that showed a strongly positive reaction. We quantified starch content from highly stained 7 soybean accessions by standard method (glucose-oxidase). As results, starch contents of 7 soybean germplasm were 2.81∼4.55%, whereas the weakly stained controls showed low starch content with less than 1%. We also measured the contents of protein, fatty acid, water potential and free sugar in 7 soybean germplasm with high starch content. High starch germplasm showed lower protein content, while low starch germplasm showed higher protein content. Our results suggest that the increasing of starch content could affect protein level in soybean seed.

Biochemical and proteomic analysis of soybean sprouts at different germination temperatures

구성철,김상곤,배동원,김현영,김현태,이영훈,강범규,백성범,백인열,윤홍태,최만수 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.3

Soybean sprouts are available throughout the year and have gained popularity as a functional food owing to their high nutritional value. In the present study, soybean seeds were germinated at different temperatures and the effects on growth characteristics, nutrient composition, and secondary metabolites were investigated. Sprout qualities such as whole length and hypocotyl length were observed to increase at a higher temperature of germination (25 vs. 20 C). The total protein content of the sprouts increased, whereas the total fatty acid content decreased upon germination at 25 C. The total phenolic content was higher in soybean sprouts than in soybean seeds. Additionally, antioxidant activity increased in a temperature-dependent manner. Both DPPH and ABTS activity were higher at 25 C than at 20 C. Proteomic analysis was conducted to generate temperature responsive protein profiles of soybean sprouts. Using 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, 33 differentially expressed spots were identified. Further analysis of these spots revealed potential function in protein storage and modification. Upon germination at 25 C, 16 spots increased significantly, whereas 17 protein spots were observed to decrease. Interestingly, a trypsin inhibitor was highly expressed at 25 C. Semi-quantitative RT-PCR analysis showed that mRNA expression level of most of genes encoding the identified proteins correlated well with their protein abundance, suggesting their temperature- dependent transcriptional regulation in soybean sprouts. In summary, our results clearly indicate an effect of temperature on growth of and secondary metabolite production in soybean sprouts.

Identification of molecular mechanism controlling P34 gene expression in soybean

구성철,Jun Su Seo,박민정,Hyun Min Cho,Mi Suk Park,Cheol Woo Choi,Wook-Hun Jung,Kyoung Hee Lee,Byung Jun Jin,김석현,심상인,정정성,정종일,김민철 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

P34 has long been known as one of majorallergenic proteins in soybean. Recently, two low-P34soybean accessions, PI603570A and PI567476, wereidentified. In order to understand the molecular mechanismof a significant reduction of P34 protein level in PI567476,we performed comparative analysis of the P34 cDNAs andgenomic sequences from low-P34 and normal soybeanaccessions. While no sequence variation was detected inP34 coding regions of the soybean accessions tested, theP34 gene from PI567476 contains ‘ATGT’ 4-bp insertionin front of the start codon. We also found the lengthpolymorphism of ‘TA’ repeats in the P34 promoter regionsamong accessions. Northern and western blot analysisrevealed that, even though mRNA levels of P34 are similarin low-P34 and normal soybean accessions, lower accumulationof P34 protein is only detected in the low-P34accession, suggesting that the genetic polymorphisms inP34 promoters have a more significant effect on translationefficiency than transcription of the P34 gene. Transientexpression analysis showed that the P34 promoter ofPI567476 has significantly reduced activity compared tothat of normal accession, cv. Clark. In addition, the chimericpromoter generated by introducing the 4-bp insertionin front of the start codon of the P34 gene from normalClark accession showed markedly reduced activity. Theresults suggest that lower accumulation of P34 protein inlow-P34 accession is largely due to the reduced translationefficiency caused by the 4-bp insertion in the P34 promoter,thus providing direct evidence for the molecularmechanism to explain the regulation of P34 gene expressionin various soybean accessions.

한중수교 30주년: 한중관계 회고와 그 미래

구성철 한국아시아학회 2022 아시아연구 Vol.25 No.1

이 논문은 한중수교 30주년을 기념해 지난 한중관계 30년을 회고하고 앞으로의 미래에 대하여 분석하였다. 한국에서의 중국의 영향력은 ‘권력의 생성과 증진’이라는 차원에서는 점진적으로 제고됐다. 그러나 ‘권위의 부재’라는 차원에서 한국에서의 중국의 영향력은 여전히 제한적인 상황임을 증명하는 것이 이 논문의 첫 번째 목적이다. 이 논문에서 필자는 지난 30년간의 한중관계의 발전과 변화를 살펴보고, 마지막 결론 부분에서는 최근 몇 년간 사드 배치와 코로나19 팬데믹 등의 이유로 한국인의 대중국 인식 변화가 일어나 한중관계가 많이 훼손되었다고 단정한다. 또한 한중관계의 훼손이 상당 기간 유지될 수밖에 없는 이유를 간략하게 정리하고 이를 극복하기 위한 세 가지 방안을 제시하였다. This paper recollects the past 30 years of Korea-China relationship and analyzes the future of it, commemorating the 30th anniversary of the diplomatic relations between Korea and China. The first goal of this paper is verifying that the influence of China on Korea has gradually increased in respect of ‘the creation and increase of power’ but still, China has limited influence on Korea in respect of ‘absence of the authority.’ In this paper, the writer looks into the development and the change of the relation between Korea and China in the past 30 years, and in conclusion affirms that the relation between Korean and China has been harmed due to the change of Koreans’ perception of China caused by deployment of THAAD and Covid-19 pandemic in the past several years. In addition, the writer states the reasons why the damage to the relation between Korean and China is to be continued for some period of time and suggests three ways to overcome the damage.

불마름병 저항성 콩 품종의 선발 및 판별

구성철, 이영훈, 김현태, 강범규, 백인열, 윤홍태, 최만수 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Bacterial pustule (BP) caused by Xanthomonas axonopodis pv. Glycines (Xag) is one of major bacterial diseases in soybean in Korea. BP causes premature defoliation and reduction in seed yield and quality. To identify soybean genotypes resistant to bacterial pustule, 58 domestic soybean cultivars for fermented soy sauce and tofu were screened. Among them 8 cultivars showed strong resistant phenotype to BP, while 7 cultivars showed susceptible phenotype. In order to determine genetic relationship between domestic soybean cultivars, seven bacterial pustule-linked simple sequence repeat (SSR) markers were selected. All of SSR markers showed polymorphism. A total 32 alleles were detected and the number of alleles per locus varied from 2-6 with an average of 4.57. The Polymorphic Information Content (PIC) values ranged from 0.513 to 0.795 in 7 loci with an average of 0.622. The genetic similarity between cultivars was analyzed with Unweighted Pair Group Method with Arithmetic mean (UPGMA) clustering. This analysis divided the cultivars into 4 major groups. A number of strong resistant and susceptible cultivars to BP were belonged to group IV and group III, respectively. In addition, 7 SSR markers successfully distinguished 56 of the 58 domestic soybean cultivars, indicating the effectiveness of BP-linked SSR markers in variety identification. The results could be useful information to discriminate soybean genotypes and help in developing elite soybean cultivars resistant to BP.

완두나물 재배에 적합한 유용자원 선발과 완두나물 적정 재배조건 설정

구성철,강범규,김현태,서정현,정광호,윤홍태,오인석,최만수 한국작물학회 2015 Korean journal of crop science Vol.60 No.4

Thirty-eight Pea (Pisum sativum L.) genotypes were screened to identify varieties to be suitable for sprout. Based on seed yield and sprout qualities such as whole length and sprout yield, five genotypes (PI269803, PI343278, PI343283, PI343300 and PI 343307) were primarily selected as candidates for pea sprouts. In order to determine optimal cultivation condition for pea sprouting, growth characteristics were investigated according to the change of germination temperature and days for sprouting. Whole length and hypocotyl length were observed to increase as a time dependent manner at each tested temperature (20, 23, and 25°C). However, whole length, hypocotyl length, and sprout yield were highly increased at 23°C compared to 20 and 25°C. Especially, PI269803 and PI343300 showed higher sprout yield than the others. In addition, the effect of the change of germination temperature on antioxidant properties was estimated by measuring total phenolic content (TPC) and free radical scavenging activity (DPPH and ABST activity). TPC and DPPH/ABST activities of PI269803 and PI343300 were higher at 23°C than at 20 and 25°C, while antioxidant properties of PI343278 and PI343283 were decreased in a temperaturedependent manner. The results show a high degree of correlation between TPC and antioxidant activities and suggest that the temperature change for pea sprouting could be responsible for antioxidant properties. Taken together, these results provide optimal cultivation conditions for pea sprouting and suggest that PI269803 and PI343300 with high sprout yield and antioxidant properties could be used for pea sprouts.