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( Wu Jie Su ),( Yan Wu ),( Hui Ling Wu ),( Wen Bing Wang ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.15 No.2
Open reading frame 4 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm4, is a gene whose function is completely unknown. With the recently developed BmNPV bacmid and a modified pFastBac1 whose polyhedrin promoter was replaced with ie1 promoter, a recombinant bacmid expressing Bm4-EGFP fusion protein under the control of ie1 promoter in BmN cells was successfully constructed. The result not only showed that the polyhedrin promoter can be replaced efficiently with other promoters to direct the expression of foreign gene in BmN cells by using Bac-to-Bac/BmNPV baculovirus expression system but also laid the foundation for rescue experiment of Bm4 deletion mutant due to the ability of ie1 promoter to direct gene expression throughout the infection cycle.
On the denominators of ε-harmonic numbers
Bing-Ling Wu,Xiao-Hui Yan 대한수학회 2020 대한수학회보 Vol.57 No.6
Let $H_n$ be the $n$-th harmonic number and let $v_n$ be its denominator. Shiu proved that there are infinitely many positive integers $n$ with $v_n =v_{n+1}$. Recently, Wu and Chen proved that the set of positive integers $n$ with $v_n = v_{n+1}$ has density one. They also proved that the same result is true for the denominators of alternating harmonic numbers. In this paper, we prove that the result is true for the denominators of $\varepsilon$-harmonic numbers, where $\varepsilon=\{ \varepsilon_i \}_{i=1}^\infty $ is a pure recurring sequence with $\varepsilon_i\in \{-1,1\}$.
Hui-Jie Fan,Zhang-Bin Tan,Yu-Ting Wu,Xiao-Reng Feng,Yi-Ming Bi,Ling-Peng Xie,Wen-Tong Zhang,Zhi Ming,Bin Liu,Ying-Chun Zhou 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2
Background: Oxidative stress-induced cardiomyocytes apoptosis is a key pathological process inischemic heart disease. Glutathione reductase (GR) reduces glutathione disulfide to glutathione (GSH) toalleviate oxidative stress. Ginsenoside Rb1 (GRb1) prevents the apoptosis of cardiomyocytes; however,the role of GR in this process is unclear. Therefore, the effects of GRb1 on GR were investigated in thisstudy. Methods: The antiapoptotic effects of GRb1 were evaluated in H9C2 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, annexin V/propidium iodide staining, and Western blotting. Theantioxidative effects were measured by a reactive oxygen species assay, and GSH levels and GR activitywere examined in the presence and absence of the GR inhibitor 1,3-bis-(2-chloroethyl)-1-nitrosourea. Molecular docking and molecular dynamics simulations were used to investigate the binding of GRb1 toGR. The direct influence of GRb1 on GR was confirmed by recombinant human GR protein. Results: GRb1 pretreatment caused dose-dependent inhibition of tert-butyl hydroperoxide-induced cellapoptosis, at a level comparable to that of the positive control N-acetyl-L-cysteine. The binding energybetween GRb1 and GR was positive ( 6.426 kcal/mol), and the binding was stable. GRb1 significantlyreduced reactive oxygen species production and increased GSH level and GR activity without altering GRprotein expression in H9C2 cells. Moreover, GRb1 enhanced the recombinant human GR protein activityin vitro, with a half-maximal effective concentration of z2.317 mM. Conversely, 1,3-bis-(2-chloroethyl)-1-nitrosourea co-treatment significantly abolished the GRb1’s apoptotic and antioxidative effects of GRb1in H9C2 cells. Conclusion: GRb1 is a potential natural GR agonist that protects against oxidative stresseinducedapoptosis of H9C2 cells.
E-Commerce Security in Rural Tourism based on Factor Analysis
Hui Song,Ling Wu 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.7
The current third-party network service in B2C business is not perfect in China, rural tourism enterprises frequent change the third-party platform because the cooperation risk. The rural tourism industry is developing fast; however, most tourism enterprises are not clear whether third-party network platform will increase the economic and social value by cooperation. Also, the third-party platform is not effective to improve the business level, and do not know how to improve the service quality. Therefore, the third-party platform should pay more attention to the service quality and perceived value, grasp the collaborative operation of cognition, improve the cooperation performance by increasing relationship quality, and promote to establish a solid and long-term cooperation relationship.
Case Reports : Sturge-Weber Syndrome
( Ling Chen ),( Jin Jin Wu ),( Min Hui Xu ),( Nian Chen ),( Ya Dong Yang ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.4
Sturge-Weber syndrome (SWS) is a neurocutaneous syndrome, characterized by the association of facial port-wine hemangiomas in the trigeminal nerve distribution area, with vascular malformation(s) of the brain (leptomeningeal angioma) with or without glaucoma. Herein, we reported Sturge-Weber syndrome in a 50-year-old man, who presented port-wine hemangiomas and epilepsy. In this case, the patient`s epilepsy episodes from his first year of life had been ignored and separated from the entity of SWS by his physicians, which led to delayed treatment. This case illustrates the importance of careful examination of patients of any age with hemangiomas in the trigeminal nerve with concomitant episodes of epilepsy. In such cases, there should be yearly neuroimaging screenings to guaranteed early interdisciplinary interventions from the time of definite diagnosis. (Ann Dermatol 23(4) 551~553, 2011)
Hui Song,Ling Wu 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.2
Along with the development of the tourism electronic commerce, we started using advanced computer network and communication technology. At the same time, scholars began to widespread concern about tourism enterprise's internal and external resources, and then expand the tourism information dissemination and promotion. In this paper, we built the curriculum theory and the practice teaching system of tourism electronic commerce based on internet, and make empirical analysis of teaching evaluation system. The result shows that multimedia teaching and management informatization is the important part of education system innovation. For tourism management, how to make use of multimedia and the practice teaching to improve students' ability is especially important. On this basis, we put forward the construction of tourism electronic commerce course, such as a combination of teaching theory and practice.
Hui SONG,Ling WU 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.3
With the continuous development of tourism education, practical teaching has become more and more important to the cultivation of tourism management professionals. At the same time, online learning becomes an important way of training tourism management professional talents and teaching method. This research focuses on the investigation of the tourism management, and tries to find out the optimal practice teaching system. Through online survey, the results prove that the practice teaching is particularly important for tourism management. Also, online classes can provide more ways of learning and vision, especially for tourism majors. Therefore, it is important to carry out network teaching, online education can improve the quality of undergraduate education; and plays an important role for constructing three dimensional modes.