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정의만,Kyong Hoon Ahn,Hyung Jin Jeon,Ha Dong Kim,Ho Sup Lee,Kwang Mook Jung,Seok Kyun Kim,Joseph V. Bonventre,김대경 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.5
The Ca^(2+)-independent phospholipase A_2 (iPLA_2) subfamily of enzymes is associated with arachidonic acid (AA) release and the subsequent increase in fatty acid turnover. This phenomenon occurs not only during apoptosis but also during inflammation and lymphocyte proliferation. In this study, we purified and characterized a novel type of iPLA_2 from bovine brain. iPLA_2 was purified 4,174-fold from the bovine brain by a sequential process involving DEAE-cellulose anion exchange, phenyl-5PW hydrophobic interaction, heparin-Sepharose affinity, Sephacryl S-300 gel filtration, Mono S cation exchange, Mono Q anion exchange, and Superose 12 gel filtration. A single peak of iPLA_2 activity was eluted at an apparent molecular mass of 155 kDa during the final Superose 12 gel-filtration step. The purified enzyme had an isoelectric point of 5.3 on twodimensional gel electrophoresis (2-DE) and was inhibited by arachidonyl trifluoromethyl ketone (AACOCF3), Triton X-100, iron, and Ca^(2+). However, it was not inhibited by bromoenol lactone (BEL), an inhibitor of iPLA2, and adenosine triphosphate (ATP). The spot with the iPLA_2 activity did not match with any known protein sequence, as determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Altogether, these data suggest that the purified enzyme is a novel form of cytosolic iPLA_2.
Amine-modified single-walled carbon nanotubes protect neurons from injury in a rat stroke model
Lee, Hyun Jung,Park, Jiae,Yoon, Ok Ja,Kim, Hyun Woo,Lee, Do Yeon,Kim, Do Hee,Lee, Won Bok,Lee, Nae-Eung,Bonventre, Joseph V.,Kim, Sung Su Springer Science and Business Media LLC 2011 Nature nanotechnology Vol.6 No.2
<P>Stroke results in the disruption of tissue architecture and is the third leading cause of death in the United States. Transplanting scaffolds containing stem cells into the injured areas of the brain has been proposed as a treatment strategy, and carbon nanotubes show promise in this regard, with positive outcomes when used as scaffolds in neural cells and brain tissues. Here, we show that pretreating rats with amine-modified single-walled carbon nanotubes can protect neurons and enhance the recovery of behavioural functions in rats with induced stroke. Treated rats showed less tissue damage than controls and took longer to fall from a rotating rod, suggesting better motor functions after injury. Low levels of apoptotic, angiogenic and inflammation markers indicated that amine-modified single-walled carbon nanotubes protected the brains of treated rats from ischaemic injury.</P>
Purification and characterization of a cytosolic Ca2+-independent phospholipase A2 from bovine brain
Jeong, Eui Man,Ahn, Kyong Hoon,Jeon, Hyung Jin,Kim, Ha Dong,Lee, Ho Sup,Jung, Sung Yun,Jung, Kwang Mook,Kim, Seok Kyun,Bonventre, Joseph V.,Kim, Dae Kyong Springer-Verlag 2011 Molecules and cells Vol.32 No.5