BMB : Reports ; An inhibitory role of NEK6 in TGFβ/Smad signaling pathway

( Jie Zuo ),( Haijie Ma ),( Hao Cai ),( Yanhua Wu ),( Wei Jiang ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.8

The NEK6 (NIMA-related kinases 6) is reported to play po-tential roles in tumorigenesis. Although it is suggested to function in several cellular pathways, the underlying mechanism in tumorigenesis is still largely unknown. In the present study, we discovered interaction of NEK6 with Smad4, a key member of transforming growth factor beta (TGFβ) pathway. Over-expression of NEK6 in hepatocellular carcinoma (HCC) cell lines suppresses TGFβ- mediated transcription activity in a kinase activity-dependent manner. In addition, NEK6 suppresses the cell growth arrest induced by TGFβ. Mechanically, NEK6 blocks nuclear translocation of Smad4, which is essential for TGF β function. Moreover, we identified that NEK6 could be regulated by TGFβ and hypoxia. Our study sheds new light on the roles of NEK6 in canonical TGFβ/Smad pathway and tum-origenesis. [BMB Reports 2015; 48(8): 473-478]

A general method for active surface adjustment of cable net structures with smart actuators

Zuo-Wei Wang,Tuan-Jie Li 국제구조공학회 2015 Smart Structures and Systems, An International Jou Vol.16 No.1

Active surface adjustment of cable net structures is becoming significant when large-size cable net structures are widely applied in various fields, especially in satellite antennas. A general-duty adjustment method based on active cables is proposed to achieve active surface adjustment or surface profile reconfiguration of cable net structures. Piezoelectric actuators and voice coil actuators are selected for constructing active cable structures and their simplified mechanical models are proposed. A bilevel optimization model of active surface adjustment is proposed based on the nonlinear static model established by the direct stiffness method. A pattern search algorithm combined with the trust region method is developed to solve this optimization problem. Numerical examples of a parabolic cable net reflector are analyzed and different distribution types of active cables are compared.

Effects of exploration and molecular mechanism of CsV on eNOS and vascular endothelial functions

Zuo, Deyu,Jiang, Heng,Yi, Shixiong,Fu, Yang,Xie, Lei,Peng, Qifeng,Liu, Pei,Zhou, Jie,Li, Xunjia Techno-Press 2022 Advances in nano research Vol.12 No.5

This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.

Magnetic, dielectric and optical properties of five-layered Aurivillius phase Bi6Fe2Ti3O18-based ceramics

Xuzhong Zuo,Enjie He,Jin Bai,Shunjin Zhu,Xucai Kan,Zhenzhen Hui,Jie Yang,Xuebin Zhu,Jianming Dai 한국물리학회 2019 Current Applied Physics Vol.19 No.12

The structural, magnetic, dielectric and optical properties of Aurivillius Bi6Fe2Ti3O18-based ceramics were investigated in detail. The replacement of Co for Fe/Ti ions obviously varies the grain morphology. Compared with Bi6Fe2Ti3O18 (BFTO) with antiferromagnetic ordering, a spin glass state can be observed in Bi5.25La0.75Fe2Ti3O18 ceramic, while other samples exhibit the ferromagnetic behavior. The specimen Bi6Fe2Ti2(NbCo)0.5O18 (BFTNCO) represents the largest remanent magnetization Mr of 0.93emu/g among all the samples, which can be attributed to the combination of a short lattice parameter c and a large lattice distortion as well as more magnetic ions in a unit cell. The room-temperature dc conductivity of BFTNCO is one order magnitude lower than that of BFTO. In addition, the band gaps of Co-doped samples are about 0.2eV smaller than these of Co-free samples. This work provides a promising path forward to tailor the multiferroic and optical properties in five-layered Aurivillius compounds.

Weighted composition operators on Bers-type spaces of Loo-Keng Hua domains

Zhi-Jie Jiang,Zuo-an Li 대한수학회 2020 대한수학회보 Vol.57 No.3

Let $\mbox{HE}_I$, $\mbox{HE}_{II}$, $\mbox{HE}_{III}$ and $\mbox{HE}_{IV}$ be the first, second, third and fourth type Loo-Keng Hua domain respectively, $\vp$ a holomorphic self-map of $\mbox{HE}_I$, $\mbox{HE}_{II}$, $\mbox{HE}_{III}$, or $\mbox{HE}_{IV}$ and $u\in H(\M)$ the space of all holomorphic functions on $\M\in\{\mbox{HE}_I, \mbox{HE}_{II}, \mbox{HE}_{III}, \mbox{HE}_{IV}\}$. In this paper, motivated by the well known Hua's matrix inequality, first some inequalities for the points in the Bers-type spaces of the Loo-Keng Hua domains are obtained, and then the boundedness and compactness of the weighted composition operators $W_{\vp,u}:f\mapsto u\cdot f\circ\vp$ on Bers-type spaces of these domains are characterized.

Structural Characterization and Thermal Behavior of a Novel Energetic Material: 1-Amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene

Ren, Xiaolei,Zuo, Xiangang,Xu, Kangzhen,Ren, Yinghui,Huang, Jie,Song, Jirong,Wang, Bozhou,Zhao, Fengqi Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel energetic material, 1-amino-1-(2,4-dinitrophenylhydrazinyl)-2,2-dinitroethylene (APHDNE), was synthesized by the reaction of 1,1-diamino-2,2-dinitroethylene (FOX-7) and 2,4-dinitrophenylhydrazine in N-methyl pyrrolidone (NMP) at 110 $^{\circ}C$. The theoretical investigation on APHDNE was curried out by B3LYP/6-311+$G^*$ method. The IR frequencies analysis and NMR chemical shifts were performed and compared with the experimental results. The thermal behavior of APHDNE was studied by DSC and TG/DTG methods, and can be divided into two crystal phase transition processes and three exothermic decomposition processes. The enthalpy, apparent activation energy and pre-exponential factor of the first exothermic decomposition reaction were obtained as -525.3 kJ $mol^{-1}$, 276.85 kJ $mol^{-1}$ and $10^{26.22}s^{-1}$, respectively. The critical temperature of thermal explosion of APHDNE is 237.7 $^{\circ}C$. The specific heat capacity of APHDNE was determined with micro-DSC method and theoretical calculation method, and the molar heat capacity is 363.67 J $mol^{-1}K^{-1}$ at 298.15 K. The adiabatic time-to-explosion of APHDNE was also calculated to be a certain value between 253.2-309.4 s. APHDNE has higher thermal stability than FOX-7.

Nonlinear structural model updating based on the Deep Belief Network

Ye Mo,Zuo-Cai Wang,Genda Chen,Ya-Jie Ding,Bi Ge 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.5

In this paper, a nonlinear structural model updating methodology based on the Deep Belief Network (DBN) is proposed. Firstly, the instantaneous parameters of the vibration responses are obtained by the discrete analytical mode decomposition (DAMD) method and the Hilbert transform (HT). The instantaneous parameters are regarded as the independent variables, and the nonlinear model parameters are considered as the dependent variables. Then the DBN is utilized for approximating the nonlinear mapping relationship between them. At last, the instantaneous parameters of the measured vibration responses are fed into the well-trained DBN. Owing to the strong learning and generalization abilities of the DBN, the updated nonlinear model parameters can be directly estimated. Two nonlinear shear-type structure models under two types of excitation and various noise levels are adopted as numerical simulations to validate the effectiveness of the proposed approach. The nonlinear properties of the structure model are simulated via the hysteretic parameters of a Bouc-Wen model and a Giuffré- Menegotto-Pinto model, respectively. Besides, the proposed approach is verified by a three-story shear-type frame with a piezoelectric friction damper (PFD). Simulated and experimental results suggest that the nonlinear model updating approach has high computational efficiency and precision.

Overtopping Failure Process and Core Wall Fracture Mechanism of a New Concrete Core Wall Dam

You-ming Zuo,Jia-wen Zhou,Hai-bo Li,Jie-yuan Zhang,Chang Tan,Xiaodong Wang,Yu-shan Wang,Yue Zhou 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.5

Concrete core wall dams have emerged as a cost-effective alternative to the reconstruction of old dams. However, the flooding mechanism and flood line of these dams differ significantly from traditional earth and rockfill dams due to the concrete core wall functioning as reinforcement. This study presents model tests that simulate the overtopping failure of earth and rockfill dams with concrete core walls of various thicknesses. The hydrologic curve and two-dimensional evolution of the breach are analyzed, and mechanical analysis examines the relationship between core wall thickness and free face during core wall failure. Results indicate that the presence of the concrete core wall shifts the overtopping failure mode to the scour pit failure mode. The scour pit failure mode occurs when upstream water scours the downstream core wall to form scour holes and free faces. Continuous scouring increases the depth of the free face, ultimately causing the moment between the two sides of the core wall to exceed the bending moment of the core wall, resulting in fracture. The study provides a theoretical basis for the design of core walls for this new type of dam.

TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction

( Suqin Shen ),( Jie Zuo ),( Huan Feng ),( Meirong Bai ),( Chenji Wang ),( Youheng Wei ),( Yanhong Li ),( Yichen Le ),( Jiaxue Wu ),( Yanhua Wu ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.6

T-complex protein 10A homolog 2 (TCP10L) was previously demonstrated to be a potential tumor suppressor in human hepatocellular carcinoma (HCC). However, little is known about the molecular mechanism. MAX dimerization protein 1 (MAD1) is a key transcription suppressor that is involved in regulating cell cycle progression and Myc-mediated cell transformation. In this study, we identified MAD1 as a novel TCP10L-interacting protein. The interaction depends on the leucine zipper domain of both TCP10L and MAD1. TCP10L, but not the interaction-deficient TCP10L mutant, synergizes with MAD1 in transcriptional repression, cell cycle G1 arrest and cell growth suppression. Mechanistic exploration further revealed that TCP10L is able to stabilize intracellular MAD1 protein level. Consistently, the MAD1-interaction-deficient TCP10L mutant exerts no effect on stabilizing the MAD1 protein. Taken together, our results strongly indicate that TCP10L stabilizes MAD1 protein level through direct interaction, and they cooperatively regulate cell cycle progression. [BMB Reports 2016; 49(6): 325-330]

WEIGHTED COMPOSITION OPERATORS ON BERS-TYPE SPACES OF LOO-KENG HUA DOMAINS

Jiang, Zhi-jie,Li, Zuo-an Korean Mathematical Society 2020 대한수학회보 Vol.57 No.3

Let HE_I, HE_II, HE_III and HE_IV be the first, second, third and fourth type Loo-Keng Hua domain respectively, 𝜑 a holomorphic self-map of HE_I, HE_II, HE_III, or HE_IV and u ∈ H(𝓜) the space of all holomorphic functions on 𝓜 ∈ {HE_I, HE_II, HE_III, HE_IV}. In this paper, motivated by the well known Hua's matrix inequality, first some inequalities for the points in the Bers-type spaces of the Loo-Keng Hua domains are obtained, and then the boundedness and compactness of the weighted composition operators W_𝜑,u : f ↦ u · f ◦ 𝜑 on Bers-type spaces of these domains are characterized.