A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

卒業定員制 實施 以後의 被學事警告者의 實態에 關한 調査硏究 : Focusing on the BNU Students of Academic Years 1981 and 1982 釜山大學校 1981, 1982學年度 入學生을 中心으로

林再澤,鄭弘燮 釜山大學校 學生生活硏究所 1982 硏究報 Vol.18 No.1

The present study is an attempt to find some informations of the under-graduate students who were probated or dismissed by BNU for unsatisfactory academic reasons since the Graduation Quota Policy. The variables considered in this research were several life backgrounds, college entrance examination score, attitudinal characteristics, and personality of the students. The subjects were 1961 students (1330 freshmen and 631 sophmores) who were probated or dismissed by the BNU regulations for Spring and Fall semesters of Academic Year 1981 and Spring semester of Academic Year 1982. The Groduation Quota Policy refers to a new educational system in which, out of 130% of college entrees, only 100% of the students are supposed to be given bacelers degree, whereas the rest of students (30%) are supposed to drop out of college programs since the Academic Year 1981 in case of every College and University in Korea. According to the implementation of this policy. students with a cumulative undergraduate course average below 1.8, or two or more grade F for two or more times before the graduation of the college are removed their name from the register by the school. The life backgrounds and the attitudinal characteristics of the subject were measured on the questionaries which were established by the Student Guidance Center at BNU, and their Personality Characteristics were measured on the Standardized Personality Diagnostic Test by Sang Ro Lee et al. These data including the subjects' college Enterance examination scores were collected at the beginning of the subjects' college entrance. As a reference, we constrasted some variables of the subjects with those of the other equivalent students including the subjects at BNU. The major findings are as follows: 1) The students who were probated or dismissed for unsatisfactory academic reasons after implementation of Grduation Quota System relatively increased more than those before the implementation of Graduation Quota System, that is, 11.7% to 1.8%, 11.2% to 1.9%, and 10.3% to 0.8% at Spring and Fall semesters of Academic Year 1981 and Spring semester of the Academic Year 1982 respectively. 2) By the analysis of data in terms of the place of residence family atmosphere, and socio-economic status, there were no significant difference between the distribution of the subjects and that of the other equivalent general students. But the propotion of the rural subjects were significantly higher that of urban subjects on the .005 level of significance. 3) There were no significant differences between the distribution of the subjects and that of the other general students according to the liberal high schools and the specialized high schools including commercial, technical, agricultural schools. 4) The less college entrance examination scores the subjects earned, the higher proportion of them were probated or dismissed by BNU concerned for unsatisfactory academic performance reasons. 5) There were a little significant relations between college entrance examination score and the subjects involved. That is, the subjects who have earned less college entrance examination scores appeared more dominant in the order of humannities, physical sciences, social sciences, engineering, and arts area. 6) Students who earned the college entrance examination scores under the lower 30 percentile in each department were equal to 41.9% of all the probated and dismissed students. and the propotion of the subjects who were higher than 20 percentile of college entrance examination score distribution of the whole students each department appeared 9.8 percent of the total subjects. 7) Where were no significant differences between the distribution of the subjects and that of the other general students in terms of types of perception of the social problems, levels of satisfaoften with their areas of study, level of interest in circle activities, and personality traits

속성 퇴비화에 관한 연구

임채규,박경수,김홍섭,노영복 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1

This experiment was conducted to fine out the rapid composting method and the effects microorganism agent. The results were summarilized as follows; 1. Each temperatures of every composts highest rised at the 5 days during the experiment composting. 2. Composting temperature of the more 60℃ was killed the harmful lavar and pathogenic microorganisms. 3. The lowest C/N ratio was No.2, among the each treatments. 4. C/N ratio of the each treatments were same results of the general compost C/N ratio. 5. The synthesized of these results were recognized the effects of microorganism agent and the each treatments were showed the more rapid composting then general compost.

수산화마그네슘에 의한 배연탈황에 관한 연구

정홍기,장성호,임찬섭,김장호 동아대학교 환경문제연구소 1995 硏究報告 Vol.18 No.2

This study has been carried out to evaluate the effect on desulfurization of flue gas by Mg(OH)₂in steam-power cogeneration plant. We investigated the effects of desulfurizatin on the temperature of flue gas and pH of circulation water in aborber. The desulfurization installations of flue gas in cogeneration and thermoelectric power plant will be increased every year because of stricted environmental regulation in Korea. Some conclusions obtained are summerized as follows ; 1. With regard to running cost, Mg(OH)₂ absorbent for desulfurization of flue gas snowed more economic than Ca(OH)₂. 2. While bunker-C oil with 1.6% sulfur was used, desulfurization of flue gas by Mg(OH)₂ was performed and then SO₂ content in the exhausted gas was under 50 ppm. 3. In flue gas desulfurization, the optimum condition on the temperature and pH was 55-60℃ and 6 respectively, then efficiency was 96%.

水稻에 있어서 葉綠素 突然變異系統의 生理·生化學的 特性에 관한 硏究

金弘燮,林采圭 조선대학교 기초과학연구소 1993 自然科學硏究 Vol.16 No.1

인위 돌연변이 유기에 의한 새로운 유전형질의 개발과 유용한 유전자 정보를 밝히기 위하여 낙동벼에서 유기된 엽록소 돌연변이 계통에 대하여 생리 생화학적 특성을 조사하였다. 얻어진 결과를 요약하면 다음과 같다. 1. 낙동벼에서 고정된 엽록소 결핍계통을 선발하였다. 이 계통은 생육초기부터 엽록소결핍으로 정상엽에 비하여 노란색을 나타내기 때문에 엽록소의 광합 성 관여 유전자 탐색에 이 용가능하다. 2. 엽록소결핍계통은 전 생육기간동안 낙동벼의 51~87% 정도의 엽록소 함량을 보였으나 엽록소 a/b함량비는 차이가 없었다. 엽 록소 결핍계통의 총 엽록소 함량은 낙동벼의 70.2%였으며 anthocy-anin함량과 flavonol함량은 각각 285% 은 142% 로 높 은 함량비를 나타냈다. 3.엽록소 결핍계통의 carotenoids함량은 낙동벼의 71.1%였고, xanthophylls함량은 56.5%였다. 특히 carotenoids성분중 lutein과 neoaxanthin함량의 감소 가 커서 각각 낙동벼의 32%와 34.4%로 매우 낮았으며, β-carotene은 차이가 없었고 antheraxanthin함량은 오히 려 증가하여 106.9%를 나타냈다. 4. 개엽상태에서나 군락상태에서 엽록소 결핍계통의 반사율과 투과율은 모두 낙동벼에 비하 여 많아 광흡수량은 떨어졌다. 5. 엽록소 결핍계통은 낙동벼에 비하여 출수기가 5일 늦고 간장, 수장, 주당수수, 수당입수, 임실율, 천립중 및 수량에서 감소하였다. 6. 엽록소 결핍계통과 낙동벼는 esterase, phosphog1ucose isomerase, malic enzyme, hexokinase 동위원소 특성에서 차이를 나타내지 않았다. 그러나 잎 에서 엽록체만을 순수분리 하여 추출한 엽록체 단백질 pattern에서는 차이를 보였다. Morphological, physiological and biochemical characteristics were examined in the mutant strains of chlorophyll induced in rice(Oryza sativa L).It was to develop a new inherited character and manifest a useful information of genes by induction of its artificial mutations. The results were summarized as follows : 1. The chlorophyll-deficiency strains fixed in the rice (Oryza sativa L.) were selected. This strains will be very of useful as a material to detect genes connected with phot-os3'llthesis of chlorophyll because of displaying yellow colors by chlorophyll-deficiency of leaver compared with normal leaves from early growth. 2. The chlorophyll-deficiency strains didn't differ between a/b content ratio of chlorophyll. Where as, 1 was shown that between but between about 51 and 87 percent contents of chlorophyll compared with the rice during all growing period. Total chlorophyll contents of these strains were measured as only 70.2% of the rice(Oryza sativa L.), but the contents of antocyanin and flavonol were highly increased to 285 and 142 percent respectively. 3. In the chlorophyll-deficiency strains compared with the rice(Oryza sativa L.), the contents of carotenoids and xanthophylls were 71 1 and 56.5 percent repectively. Especially in the carotenoid ingredients, the contents of lutein and neoxanthin highly decreased to 32 and 34.4 persent respectively, but the contents of β-carotene were equal and the contents of antheraxanthins increased to 106.7 percent. 4. In the individual or community condition, the reflexiblity and transmission of the chlorophylldefieiency strains increased and its absorptivity decreased in comparision with the rice(Oryza sativa L. ) 5. In the chlorophyll-deficiency strains compared with the rice(0ryza sativa L.), the earing season was fivedays late, culm length, panicle length, number of panicle perhill, number of spikelets per panicle, ripened grain ratio and 1000-grain weight and vield decreased. 6. The chloroph11-deficiency strains and rice(0ryza sativa L.) differed little in the characteristics of isoenzymes, esterase, phosphogluicose, isomerase, malic enzyme and hexokinase. But the protein pattern of chloroplast extracted by pure seperation differed.

A. 高校 科學 (生物·地球科學) 및 數學科 探究學習의 評價模型 定立과 評價紙 開發

李相協,權炳奎,林在圭,孫文求,吳岱燮,楊洪準,梁承榮,姜用熙,林成圭,金正遠,金吉民,兪正在 경북대학교 과학교육연구소 1982 科學敎育硏究誌 Vol.6 No.-

Currently the evaluation of high school science and mathematics learning has been too much knowledge-centered without concerning the inquiring ability or scientific attitude. This study, therefore, was made for the purpose of establishing of evaluation model for high school science (biology and earth science) and mathematics learning and its evaluation items. In this paper, the research was done about three fields, biology, earth science and mathematics. 1. For the biology, the evaluation methods of inquiry learning were studied with the foreign references, and proper evaluation items were suggested. 2. For the earth science, after analizing 5 kinds of earth science textbook of current using in high school, essential 19 items were selected. 5 of them were tested in test group and control group, then, the results were analized. 3. For the mathematice, the teaching elements of「Set」and「Flow chart」in mathematics (Ⅰ) were selected and structured. Detailed teaching objects were established and evaluation items were introduced.

Characterization of the in vitro Activities of the P1 and Helper Component Proteases of Soybean mosaic virus Strain G2 and Tobacco vein mottling virus

Lim, Hyoun-Sub,Jang, Chan-Yong,Nam, Ji-Ryun,Li, Meijia,Hong, Jin-Sung,Bae, Han-Hong,Ju, Ho-Jong,Kim, Hong-Gi,Ford, Richard E.,Domier, Leslie L. The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.2

Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HC-Pro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed poly-proteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV-and TVMV-encoded proteases are differentially sensitive to protease inhibitors.

Soybean mosaic virus Infection and Helper Component-protease Enhance Accumulation of Bean pod mottle virus-Specific siRNAs

Lim, Hyoun-Sub,Jang, Chan-Yong,Bae, Han-Hong,Kim, Joon-Ki,Lee, Cheol-Ho,Hong, Jin-Sung,Ju, Ho-Jong,Kim, Hong-Gi,Domier, Leslie L. The Korean Society of Plant Pathology 2011 Plant Pathology Journal Vol.27 No.4

Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV and Soybean mosaic virus (SMV) and in transgenic soybean plants expressing SMV helper component-protease (HC-Pro). In many potyviruses, HC-Pro is a potent suppressor of RNA silencing. In plants infected with BPMV alone, accumulation of siRNAs was positively correlated with symptom severity and accumulation of BPMV genomic RNAs. Plants infected with both BPMV and SMV and BPMV-infected transgenic soybean plants expressing SMV HC-Pro exhibited severe symptoms characteristic of BPMVSMV synergism, and showed enhanced accumulation of BPMV RNAs and siRNAs compared to plants infected with BPMV alone and nontransgenic plants. Likewise, SMV HC-Pro enhanced the accumulation of siRNAs produced from a silenced green fluorescent protein gene in transient expression assays, while the P19 silencing suppressor of Tomato bushy stunt virus did not. Consistent with the modes of action of HC-Pro in other systems, which have shown that HC-Pro suppresses RNA silencing by preventing the unwinding of duplex siRNAs and inhibiting siRNA methylation, these studies showed that SMV HC-Pro interfered with the activities of RNA-induced silencing complexes, but not the activities of Dicer-like enzymes in antiviral defenses.

[Free Paper] Liver resection to Bismuth Type I and Type II cholangiocarcinoma

Jin Hong Lim,Gi Hong Choi,Sung Hoon Choi,Sung Hoon Kim,Jin Sub Choi,Kyung Sik Kim 대한외과학회 2011 대한외과학회 학술대회 초록집 Vol.2011 No.11

알코올 중독 환자의 심리 유형과 임상적 특성 간의 관계에 대한 예비적 연구

임홍식(Hong-Sik Lim),이미경(Mi-Kyung Lee),박영숙Young-Sook Park),최정윤(Jung-Yun Choi),홍성곤(Sung-Kon Hong),최상섭Sang-Sub Choi),이재갑(Jae-Kap Lee) 한국중독정신의학회 2002 중독정신의학 Vol.6 No.1

Objectives:This study has been carried out in order to understand the degree of preference of the psychological types of the alcoholics and their sociodemographic variables and then to verify the correlation of these variables using statistical methods, which will be the preliminary keys to the dynamic understanding of the drug addiction. Methods:We have conducted a questionnaire survey of the 31 alcoholics, which had questions about MBTI and sociodemographic variables. The results were statistically analyzed using SPSS. Results:Most of the them were in their thirties, with little school background, and under poor economic condition. Many of them were second sons in their family and many of them had only mother, while father was dead. There was a relative tendency, even not so prominent in a statistical sense, that many of the alcoholics were extroverts, and that they liked thinking function, and preferred the sensing function. Conclusion:We hope that this preliminary study will be a starting point for a deeper study which will reveal the relations between the addiction to alcohol and the degree of the preference of the psychological types, stereotyping, and the dynamic meaning.