Simultaneous Single-Position Lateral Lumbar Interbody Fusion Surgery and Unilateral Percutaneous Pedicle Screw Fixation for Spondylolisthesis

Hui Lv,Yu Sheng Yang,Jian Hong Zhou,Yuan Guo,Hui Chen,Fei Luo,Jian Zhong Xu,Zhong Rong Zhang,Ze Hua Zhang 대한척추신경외과학회 2023 Neurospine Vol.20 No.3

Objective: To evaluate the clinical and radiological efficacy of a combine of lateral single screw-rod and unilateral percutaneous pedicle screw fixation (LSUP) for lateral lumbar interbody fusion (LLIF) in the treatment of spondylolisthesis. Methods: Sixty-two consecutive patients with lumbar spondylolisthesis who underwent minimally invasive (MIS)-TLIF with bilateral pedicle screw (BPS) or LLIF-LSUP were retrospectively studied. Segmental lordosis angle (SLA), lumbar lordosis angle (LLA), disc height (DH), slipping percentage, the cross-sectional areas (CSA) of the thecal sac, screw placement accuracy, fusion rate and foraminal height (FH) were used to evaluate radiographic changes postoperatively. Visual analogue scale (VAS) and Oswestry Disability Index (ODI) were used to evaluate the clinical efficacy. Results: Patients who underwent LLIF-LSUP showed shorter operating time, less length of hospital stay and lower blood loss than MIS-TLIF. No statistical difference was found between the 2 groups in screw placement accuracy, overall complications, VAS, and ODI. Compared with MIS-TLIF-BPS, LLIF-LSUP had a significant improvement in sagittal parameters including DH, FH, LLA, and SLA. The CSA of MIS-TLIF-BPS was significantly increased than that of LLIF-LSUP. The fusion rate of LLIF-LSUP was significantly higher than that of MIS-TLIF-BPS at the follow-up of 3 months postoperatively, but there was no statistical difference between the 2 groups at the follow-up of 6 months, 9 months, and 12 months. Conclusion: The overall clinical outcomes and complications of LLIF-LSUP were comparable to that of MIS-TLIF-BPS in this series. Compared with MIS-TLIF-BPS, LLIF-LSUP for lumbar spondylolisthesis represents a significantly shorter operating time, hospital stay and lower blood loss, and demonstrates better radiological outcomes to maintain lumbar lordosis, and reveal an overwhelming superiority in the early fusion rate.

Suppression of Cellular Apoptosis Susceptibility (CSE1L) Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells

Zhu, Jin-Hui,Hong, De-Fei,Song, Yong-Mao,Sun, Li-Feng,Wang, Zhi-Fei,Wang, Jian-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

The cellular apoptosis susceptibility (CSE1L) gene has been demonstrated to regulate multiple cellular mechanisms including the mitotic spindle check point as well as proliferation and apoptosis. However, the importance of CSE1L in human colon cancer is largely unknown. In the present study, we examined expression levels of CSE1L mRNA by semiquantitative RT-PCR. A lentivirus-mediated small interfering RNA (siRNA) was used to knock down CSE1L expression in the human colon cancer cell line RKO. Changes in CSE1L target gene expression were determined by RT-PCR. Cell proliferation was examined by a high content screening assay. In vitro tumorigenesis was measured by colony-formation assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis. We found CSE1L mRNA to be expressed in human colon cancer cells. Using a lentivirus based RNAi approach, CSE1L expression was significantly inhibited in RKO cells, causing cell cycle arrest in the G2/M and S phases and a delay in cell proliferation, as well as induction of apoptosis and an inhibition of colony growth capacity. Collectively, the results suggest that silencing of CSE1L may be a potential therapeutic approach for colon cancer.

Simple synthesis of Biphosphonates with Excellent Flameproofing Properties

( Hui Zhen Zhi ),( Yang Cao ),( Wei Wei Zhang ),( Xu Feng Yang ),( Jin Fei Yang ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.2

Four excellent halogen-free biphosphonate flame retardants (EBDMP, EBDEP, MBDMP, MBDEP) were synthesized through poly condensation and Arbuzov rearrangement reactions without using any solvents. The products were characterized by ESI-MS, FT-IR and 1H NMR. The application of EBDEP in polyurethane soft foam was studied and the results illustrated its excellent flame-proofing properties.

CCR6 Is a Predicting Biomarker of Radiosensitivity and Potential Target of Radiosensitization in Rectal Cancer

Hui Chang,Jia-wang Wei,Ya-lan Tao,Pei-rong Ding,Yun-fei Xia,Yuan-hong Gao,Wei-wei Xiao 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4

Purpose This study aimed to explore the functions and mechanisms of C-C motif chemokine receptor 6 (CCR6), a gene associated with progression and metastasis of colorectal cancer (CRC), in radiosensitivity of rectal cancer (RC). Materials and Methods RNA sequencing and immunohistochemical analysis on CCR6 expression were performed in pretreatment tissues of RC patients exhibiting different therapeutic effects of radiotherapy. Colonogenic survival assay was conducted in different CRC cell lines to assess their radiosensitivity. And the impact of CCR6 expression on radiosensitivity was validated through RNA interference. The DNA damage repair (DDR) abilities of cell lines with different CCR6 expression were evaluated through immunofluorescence-based H2AX quantification. Results The CCR6 mRNA level was higher in patients without pathologic complete remission (pCR) than in those with pCR (fold changed, 2.11; p=0.004). High-level expression of CCR6 protein was more common in the bad responders than in the good responders (76.3% vs. 37.5%, p < 0.001). The CRC cell lines with higher CCR6 expression (LoVo and sw480) appeared to be more radioresistant, compared with the sw620 cell line which had lower CCR6 expression. CCR6 knockdown made the LoVo cells more sensitive to ionizing radiation (sensitization enhancement ratio, 1.738; p < 0.001), and decreased their DDR efficiency. Conclusion CCR6 might affect the RC radiosensitivity through DDR process. These findings supported CCR6 as a predicting biomarker of radiosensitivity and a potential target of radiosensitization for RC patients.

Media Optimization for Laccase Production by Trichoderma harzianum ZF-2 Using Response Surface Methodology

( Hui Ju Ga ),( Xiang Chu ),( Yan Wen Wang ),( Fei Zhou ),( Kai Zhao ),( Zhi Mei Mu ),( Qing Xin Liu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12

Trichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-atime method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal, and CuSO4 were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l, soybean meal 23.07 g/l, (NH4)2SO4 1 g/l, CuSO4 0.51 g/l, Tween-20 1 g/l, MgSO4 1 g/l, and KH2PO4 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.

Electrical current induced mechanism in microstructure and nano-indention of Al–Zn–Mg–Cu (AZMC) Al alloy thin film

Fei-Yi Hung,Jiunn-Der Liao,Truan-Sheng Lui,Li-Hui Chen 한국물리학회 2011 Current Applied Physics Vol.11 No.6

In this study, the microstructural variation and nano-indention of Al-5.7Zn-2.4Mg-1.5Cu (AZMC) thin film was investigated using DC electrical current at a density of 1000 A/cm^2. The results show that microstructural changes due to the electrical current involved both the solid solubility effect and enhanced diffusion. The electrical current drove the Al atoms and Cu atoms of the matrix from the cathode to the anode. After electrical current testing, precipitation phases (Al_2Cu; CuMgAl_2) had decomposed into the cathode matrix and MgZn phases had grown in the anode zones. Meanwhile, the current also caused the hardness of the thin film to decrease and affected both the texture and dynamic strain mechanism of nano-indention.

Controlling Ejection State of a Pneumatic Micro-droplet Generator Through Machine Vision Methods

Fei Wang,Yiwei Wang,Weijie Bao,Hui Zhang,Jiangeng Li,Zhihai Wang 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.4

Pneumatic micro-droplet ejection is a printing technique that has potential applications in many fi elds, especially in thefi eld of bio-printing. The ejection is via a solenoid valve being briefl y turned on, so that high pressure gas enters the liquidreservoir, forming a gas pressure pulse, forcing the liquid out through a tiny nozzle to form a micro-droplet. For bio-printingapplications, the bio-inks are typically non-standard. The diffi culties are not only that the initial working parameters arediffi cult to set, but also the working conditions change over time in many cases. In order to maintain a stable single-dropejection state, a machine vision based ejection monitoring was designed to obtain the number, positions and sizes of thedroplets for each ejection, and a feedback control is realized by adjusting the “ON” time of the solenoid valve or the gaspressure at the front end of the solenoid valve.

Non-Doped Organic Light-Emitting Diodes with Saturated Red Emission

Fei Xiao,Bing-xian Shao,Huan-rong Wu,Hui-ying Fu,Xiao-yuan Hou,Xin-dong Gao,Yi-qiang Zhan 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.2

Non-doped organic light-emitting diodes with saturated red emission were fabricated using 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyldi(1-naphthyl)amine (DNP-2CN) or 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyl(1-naphthyl)phe- nylamine (DPN-2CN) as the emitting layer. Different electron-transporting materials, tris(8-hydroxylquinoline) aluminum (Alq$_3$), 2,2',2''-(1,3,5-phenylene)tris[1-phenyl-1$H$-benzimidazole] (TPBI) and 2-(4-biphenyl)-5-(4-tert-butylphenyl)-1,3,4-oxadiazole (PBD), were introduced into the devices for examining their energy level compatibility of DNP-2CN or DPN-2CN. The device with a structure of ITO/ NPB/ DNP-2CN/ BCP/ Alq$_3$/ LiF/ Al showed red emission with $\lambda_{max}$ at 670 nm (CIE coordinates: $x$ = 0.66, $y$ = 0.33) and a high luminance of 438 cd m$^{-2}$ at a driving voltage of 12 V. The device with a structure of ITO/ NPB/ DPN-2CN/ BCP/ Alq$_3$/ LiF/ Al showed a high brightness of 225 cd m$^{-2}$ at a driving voltage of 12 V with $\lambda_{max}$ at 674 nm (CIE coordinates: $x$ = 0.65, $y$ = 0.33).

Contributed Mini Review : Role of Wnt signaling in fracture healing

( Hui Yun Xu ),( Jing Duan ),( Dan Dan Ning ),( Jing Bao Li ),( Ruo Fei Liu ),( Rui Xin Yang ),( Jean X Jiang ),( Peng Shang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.12

The Wnt signaling pathway is well known to play major roles in skeletal development and homeostasis. In certain aspects, fracture repair mimics the process of bone embryonic development. Thus, the importance of Wnt signaling in fracture healing has become more apparent in recent years. Here, we summarize recent research progress in the area, which may be conducive to the development of Wnt-based therapeutic strategies for bone repair.

Material Removal Mechanisms of Cu-Co Metal-Powder Composite by Microorganisms

Fei Ma,Hui Huang,Xipeng Xu 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.5

Material characteristics of pure copper, pure cobalt and Cu–Co metal-powder composite were comparative studied through biomachining by Acidithiobacillus ferrooxidans (A. ferrooxidans) in three groups of culture solutions. Material removal mechanisms of Cu–Co metal-powder composite involved in biomachining were explored. It was first observed that the pure cobalt and Cu–Co metal-powder composite were machined by the A. ferrooxidans. The material removal of three workpieces presented linear increase along the machining process due to the metabolic activity of the A. ferrooxidans. Different with the pure metal, the material removal mechanism of Cu–Co metal-powder is explained by the dual eff ect of micro-galvanic corrosion and participation of Cu 2+ and Fe 3+ for oxidization of cobalt.