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( Bog Ja Jeoung ),( Yu Jin Jeong ),( Eun Bin Kim ),( Eun Sol Cho ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1
Purpose : In previous researches, we learned that exercising promotes physical fitness ,strength and positive change in the physical satisfaction level but reviewing previous researches, there was a limit in our finding as it was based on a singular physical activity or exercise and we could not exactly find out which exercise provided the most physical change and satisfaction level. Therefore, the purpose of this study was to find out effects of physical fitness and satisfaction according to participate in exercise method(circuit strength, weight bearing program and badminton). Method: Twelve college students participated exercise program(circuit strength program : n=4, weight bearing program : n=4 and badminton : n=4) who measured 20m PACER, push-up, grip strength, sit-up and sit-reach and body composition, and satisfaction which ``Sports Participants Satisfaction Questionnaire`` developed by Yoo et al(2010). The questionnaire covered improving physical function (3), improved exercise capacity (3), promote social and psychological benefits (3), two healthy living (2) which consisted of total of 14 questions with 5 multiple choices. With regard to the body satisfaction level survey, we have used the BSQ: Body Shape Questionnaire developed in 1987 by Cooper, Taylor and Fairvurn. Park, Eun-ah and Noh, Young-kyung was edited the BSQ questionnaire for this research. The body satisfaction level survey consisted of four sub-variables and a total of 27 questions. Result: First, there is a high level of increase overall in physical strength measurement results especially in sit-ups, flexibility, push-ups and cardiovascular endurance. However, there was little difference between the groups in terms stamina changes while we could not compare physical strength changes based on the type of exercise. Second, there was a slight decrease in weight, BMI, waist and hip circumference with regard to physical changes. However, the physical changes between groups were small while there was no physical change based on the type of exercise. In addition, the was no changes in the level of satisfaction based on after and before exercising while there was only slight difference exercise groups. Third, overall satisfaction level showed significant increase among the exercise groups while the health exercise showed the highest level of satisfaction followed by badminton and circuit training. In terms of exercise satisfaction level by category, physical function improvement and improved exercise capacity showed the highest level of increase and there was little difference in the exercise satisfaction level based on the exercise type. Fourth, if we look at the satisfaction level for physical strength change, satisfaction level was high for strength and agility category but showed no changes between exercise groups. Conclusion: This research is the study of the satisfaction level and changes in physical and physical strength based on three different exercises for different exercise groups. Based on the results of the study, exercising regularly helps increase the overall physical level in all exercise groups and the satisfaction level gradually increases. However, there was little difference between the exercise groups and this research was unable to verify the difference based on the exercise groups. Since the research was limited to 12 participants, a follow-up research should be conducted based on an extended level of participants.
Generation of Soluble Human Tumor Necrosis Factor-α Receptor 1-Fc Transgenic Pig
Cho, Bumrae,Koo, Ok Jae,Hwang, Jong-Ik,Kim, Hwajung,Lee, Eun Mi,Hurh, Sunghoon,Park, Sol Ji,Ro, Han,Yang, Jaeseok,Surh, Charles D.,d’Apice, Anthony J.,Lee, Byeong Chun,Ahn, Curie Lippincott Williams Wilkins, Inc. 2011 Transplantation Vol.92 No.2
BACKGROUND.: Acute humoral xenograft rejection (AHXR) is an important barrier to xenograft survival. Human tumor necrosis factor-α (hTNF-α) is one of the essential mediators of AHXR and induces activation of porcine endothelial cells (PECs), resulting in upregulation of major histocompatibility complex molecules, adhesion molecules, and proinflammatory chemokines. We investigated whether introduction of a soluble human tumor necrosis factor receptor I-Fc (shTNFRI-Fc) fusion gene can suppress activation of PECs and, more importantly, produced shTNFRI-Fc transgenic pigs. METHODS.: The shTNFRI-Fc gene expression vector was constructed and inserted into PECs. The inhibitory effects of shTNFRI-Fc were tested by luciferase assay, reverse-transcriptase polymerase chain reaction, and flow cytometry. A shTNFRI-Fc transgenic pig was generated by somatic cell nuclear transfer. The expression of shTNFRI-Fc in the transgenic pig was evaluated by PCR, western blot, enzyme-linked immunosorbent assay, and immunohistochemistry. The inhibitory effects of shTNFRI-Fc in the serum obtained from the transgenic pig were also tested. RESULTS.: In comparison with control green fluorescent protein, shTNFRI-Fc protein showed much stronger inhibitory effects on NF-&kgr;B activation in the HEK293-NF-&kgr;B-luciferase reporting cell line, expression of chemokines and adhesion molecules in PECs, and TNF-α-mediated cytotoxicity. We successfully generated shTNFRI-Fc transgenic pig. Sera obtained from the transgenic pig inhibited induction of chemokines, and E-selectin in PECs stimulated with Human TNF-α. CONCLUSIONS.: We have generated transgenic pigs producing shTNFRI-Fc protein that can inhibit TNF-α-mediated activation of PECs. Because TNF-α is an important mediator of xenograft rejection, the use of xenografts that can produce shTNFRI-Fc proteins de novo could be an effective approach in overcoming a considerable component of the xenograft rejection process, especially AHXR.
Han-Sol Park,전병환,Sung Hoon Woo,Jaechan Leem,Jung Eun Jang,Min Sock Cho,박인선,이기업,고은희 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.6
Methionine and choline-deficient diet (MCD)-induced fatty liver is one of the best-studied animal models of fatty liver disease. The present study was performed to clarify the relative contributions of individual lipid metabolic pathways to the pathogenesis of MCD-induced fatty liver. Hepatic lipogenesis mediated by the sterol regulatory element-binding protein (SREBP-1c) was increased at 1 week, but not at 6 weeks, of MCD feeding. On the other hand, ^(14)C-palmitate oxidation did not change at 1 week, but significantly decreased at 6 weeks. This decrease was associated with increased expression of fatty acid translocase, a key enzyme involved in fatty acid uptake. Expression of endoplasmic reticulum stress markers was increased in mice given MCD for both 1 and 6 weeks. These findings suggest the presence of time-dependent differences in lipid metabolism in MCD-induced fatty liver disease: SREBP-1c-mediated lipogenesis is important in the early stages of fatty liver disease, whereas increased fatty acid uptake and decreased fatty acid oxidation become more important in the later stages.
환기시스템에 따른 강제환기식 돈사의 에너지 절감 효과 분석: 한국식 및 유럽식 환기
강솔뫼 ( Sol-moe Kang ),이인복 ( In-bok Lee ),조정화 ( Jeong-hwa Cho ),최영배 ( Young-bae Choi ),정효혁 ( Hyo-hyeog Jeong ),김다인 ( Da-in Kim ),조윤우 ( Yun-woo Cho ),이은주 ( Eun-ju Lee ) 한국농공학회 2023 한국농공학회 학술대회초록집 Vol.2023 No.0
한국에서의 고기 소비 수요가 계속 증가함에 따라 돈사 시설은 점점 대형화되고 있으며 사육형태는 밀집 사육으로 바뀌고 있다. 그로 인해 돈사의 내부환경은 열악해지고 있으며, 적절한 사육환경을 조성하기 위해서는 환기 제어가 필요하다. 따라서, 적절한 사육환경 조성을 위해 많은 돈사 시설이 자연 환기에서 강제 환기로 바뀌고 있다. 최근, 유럽에서 개발한 피트 채널 입기 형태의 돈사 시설이 국내에 신축되고 있지만, 그에 따른 냉난방 에너지 효율에 관한 연구는 아직 미미합니다. 따라서, 본 연구에서는 다양한 강제 환기시스템의 냉난방 에너지 부하를 분석하여 강제 환기식 돈사의 에너지 효율성을 평가하였다. 건물에너지 시뮬레이션(Building Energy Simulation, BES) 기술을 사용하여 강제환기식 돈사의 냉난방 에너지 부하를 모의하기 위한 모델을 만들었다. 두 개의 농장에서 돈방 내부 온도와 피트 채널 전후의 온도에 대해 각각 현장실험을 수행하여 BES 모델을 검증하였다. 다양한 환기시스템을 분석하기 위한 시뮬레이션 모델은 농림식품부와 농협에서 개발한 2016년 축사표준설계도를 기반으로 설계하였다. 최종적으로 환기시스템과 지역에 따라 총 72개의 케이스를 구성하여 냉난방 에너지를 분석하였다. 지역에 관계없이 유럽형 환기 구조인 피트 채널 입기 구조에서 냉난방 에너지 효율이 가장 높았으며, 배기 구조에 따른 냉난방 에너지의 변화는 거의 없었다. 복도가 없고 중천장이 없는 내부 구조는 냉방 에너지 효율이 가장 높게 나타났으며, 평평한 중천장과 중앙 복도 구조는 난방 에너지 효율이 가장 높게 나타났다. 따라서, 제주에서는 피트 채널 입기를 사용하며 복도와 중천장이 없는 구조에서 7.3%로 가장 큰 냉난방 에너지 절감효과를 보였다. 홍성과 포천에서는 피트 채널 입기를 사용하며 평평한 중천장과 중복도 구조에서 각각 9.6%와 10.6%로 가장 큰 냉난방 에너지 절감효과를 보였다.
Genetic Mapping of green-stripe in Tomato
Woon Cho,Eun Sol Kang,Je Min Lee 한국원예학회 2021 원예과학기술지 Vol.39 No.3
Tomato (Solanum lycopersicum) has a high level of genetic variation in fruit color, which is an important fruit quality trait considered by breeders and consumers. The fruit stripe color pattern is determined by the green stripe (gs) locus and is observed only in the peel tissue in tomato. Using two different F₂ populations (351 and 97 individuals), we mapped gs to the long arm of chromosome 7 and found that it cosegregated with TOMATO AGAMOUS-LIKE 1 (TAGL1). Association analysis using a TAGL1-specific derived cleaved amplified polymorphic sequences (dCAPS) marker distinguished 118 non-green-striped and 11 green-striped tomato cultivars. The chlorophyll content of green-striped peels (GSPs) was higher than that of light-green peels (LGPs), and the carotenoid content of red peels (RPs) was higher than that of yellow-striped peels (YSPs). Although we did not find sequence variation in the promoter and coding regions of TAGL1 between non-striped and striped tomatoes, TAGL1 expression in the YSPs was lower than that in the RPs. Therefore, the differential expression of TAGL1 may contribute to fruit stripe patterning. Marker-assisted selection using the TAGL1-specific marker identified here will facilitate breeding of fruit stripe patterning in tomato.