실내 동적 환경에서의 SLAM 및 딥러닝 기반 미아 발견 시스템

김은비(Eun-Bee Kim),최영은(Yeong-Eun Choi),강수현(Su-Hyeon Kang),김소연(So-Yeon Kim),김해영(Hae-Yeong Kim),신수용(Soo-Young Shin) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.11

Label-free and rapid colorimetric detection of pathogen using specific aptamer based on enzyme-like activity of magnetic nanoparticles

Ji Young Park,Ha Young Jung,Seung Hun Back,Eun Joo Kim,Ju Hee Lee,Bo Bae Kim,Seon Ah Cheon,Eun Bee Kim,Min Young Joo,Seung Woo Lee,Ji Min Seo,Myung Sun Kim,Gi Wook Kim,Moon Il Kim,Tae Jung Park 한국진공학회 2014 한국진공학회 학술발표회초록집 Vol.2014 No.8

GG-05 : The comparison of cervical elongation with uterine prolapse; Clinical correlations

( Eun Bee Noh ),( Su Yeon Park ),( Soo Rim Kim ),( Yeo Jung Moon ),( Sei Kwang Kim ),( Sang Wook Bai ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

The descent of uterine cervix is one of the important findings of apical prolapse and can be seen in cervical elongation or uterine prolapse patients with Valsalva for a clinic examination. The objective of this study is to compare the clinical characteristics of cervical elongation with that of uterine prolapse without cervical elongation. A retrospective chart review of women undergoing cervix amputation or vaginal hysterectomy for pelvic organ prolapse between January 2005 and February 2013 at Yonsei University Health System was conducted. Based on the pelvic organ prolapse quantification (POP-Q) examination, 30 women with cervical elongation (cases, without uterine prolapse) and 29 women with uterine prolapse (controls, without cervical elongation) were recruited. Cervical elongation was defined as the difference between point C and point D of POP-Q system greater than 4 cm, and uterine prolapse as the point D descent greater than -1 cm. In case group, 16 patients (53.3%) had cervix amputation surgery, 12 (40.0%) had vaginal hysterectomy and the rest 2 patients had total abdominal hysterectomy. Vaginal hysterectomy was performed in all patients in control group. The mean cervix length was 6.9±1.9 cm (mean±SD) in cervix elongation group and 0.2±0.7 cm in control group (p<0.001). There were significant differences in age (67±6.9 vs. 51.6±12.8 years, p<0.001), height (152.8±5.1 vs. 156.5±5.2 cm, p=0.008), vaginal parity (3.1±0.9 vs. 2.1±0.8, p<0.001), difficult labor history (72.4% vs.46.7%, p=0.044), postmenopuasal status (96.6% vs. 40.0%, p<0.001) and diabetes mellitus (31% vs. 6.7%, p=0.016) in demographics. There were no significant differences in urinary symptoms (leakage, frequency, urgency, nocturia and postvoiding fullness) and physical examinations (Q-tip and stress tests). In POP-Q system, there were significant differences in stages (III vs. IV, OR 0.125, 95% CI 0.035-0.451, p=0.001) and point Aa (2.7±1.1 vs. -1.3±2.3 cm, p<0.001), Ba (4.6±2.0 vs. -1.2±2.7 cm, p<0.001), D (3.4±2.3 vs. 4.0±2.1 cm, p<0.001), Ap (1.8±1.2 vs. -0.5±1.5 cm, p<0.001), and Bp (2.8±2.6 vs. -0.4±1.7 cm, p<0.001). In the multivariate logistic regression model, only POP-Q point Ap (OR 0.042, 95% CI 0.003-0.514) remained statistically significant. Women with cervical elongation have less descent of posterior vaginal wall than those with uterine prolapse.

Both CD45RA⁺ and CD45RO⁺ human CD4⁺ T cells drive direct xenogeneic T-cell responses against porcine aortic endothelial cells

Kim, Chi Hwa,Oh, Keunhee,Kim, Dong-Eun,Lee, Seul Bee,Yang, Ji Hye,Lee, Gene,Cho, Jaejin,Lee, Dong-Sup Blackwell Publishing Ltd 2010 Xenotransplantation Vol.17 No.3

<P>Kim CH, Oh K, Kim D-E, Lee SB, Yang JH, Lee G, Cho J, Lee D-S. Both CD45RA<SUP>+</SUP> and CD45RO<SUP>+</SUP> human CD4<SUP>+</SUP> T cells drive direct xenogeneic T-cell responses against porcine aortic endothelial cells. Xenotransplantation 2010; 17: 224–232. © 2010 John Wiley & Sons A/S.</P><P>Abstract: Background: </P><P>Xenogeneic cellular immune responses are mediated by either direct or indirect pathways depending on the participation of donor or host antigen presenting cells, respectively. The contribution of direct response of human T cells, especially memory T cells, to porcine antigen presenting cells is currently unknown. Here, we sought to determine whether human peripheral blood memory/activated phenotype T cells are directly responsive to porcine endothelial cells.</P><P>Methods: </P><P>Porcine aortic endothelial cells (PAECs) were prepared from Yorkshire or miniature pigs. Highly purified human T cells, including naïve and memory/activated phenotype cells, were incubated with PAECs with or without the addition of exogenous cytokines. T-cell proliferation and T-cell receptor (TCR) V&bgr; usage in response to PAECs were analyzed.</P><P>Results: </P><P>Both CD8<SUP>+</SUP> and CD4<SUP>+</SUP> T cells responded directly to PAECs and exhibited exclusive responsiveness to SLA class I and class II molecules, respectively. Naïve and memory/activated phenotype CD4<SUP>+</SUP> T cells responded against PAECs, whereas only naïve phenotype CD8<SUP>+</SUP> T cells contributed to such a response. In addition, both populations of xenogeneic human CD4<SUP>+</SUP> T cells exhibited similar and diverse V&bgr; usage.</P><P>Conclusion: </P><P>Due to the considerable contribution of human CD45RO<SUP>+</SUP>CD4<SUP>+</SUP> T cells to the xenoreactivity against PAECs, effective control of xenogeneic memory/activated T-cell responses would significantly affect long-term survival of transplanted grafts.</P>

Improved <i>In Vivo</i> Stability of Radioiodinated Rituximab Using an Iodination Linker for Radioimmunotherapy

Kim, Eun Jung,Kim, Byoung Soo,Choi, Dan Bee,Chi, Sung-Gil,Choi, Tae Hyun Mary Ann Liebert 2016 Cancer Biotherapy & Radiopharmaceuticals Vol.31 No.8

Early detection of the growth of <i>Mycobacterium tuberculosis</i> using magnetophoretic immunoassay in liquid culture

Kim, Jeonghyo,Lee, Kil-Soo,Kim, Eun Bee,Paik, Seungwha,Chang, Chulhun L.,Park, Tae Jung,Kim, Hwa-Jung,Lee, Jaebeom Elsevier Applied Science 2017 Biosensors & bioelectronics Vol. No.

<P><B>Abstract</B></P> <P>Tuberculosis (TB) is an often neglected, epidemic disease that remains to be controlled by contemporary techniques of medicine and biotechnology. In this study, a nanoscale sensing system, referred to as magnetophoretic immunoassay (MPI) was designed to capture culture filtrate protein (CFP)-10 antigens effectively using two different types of nanoparticles (NPs). Two specific monoclonal antibodies against CFP-10 antigen were used, including gold NPs for signaling and magnetic particles for separation. These results were carefully compared with those obtained using the commercial mycobacteria growth indicator tube (MGIT) test via 2 sequential clinical tests (with ca. 260 clinical samples). The sensing linearity of MPI was shown in the range of pico- to micromoles and the detection limit was 0.3pM. MPI using clinical samples shows robust and reliable sensing while monitoring <I>Mycobacterium tuberculosis</I> (MTB) growth with monitoring time 3–10 days) comparable to that with the MGIT test. Furthermore, MPI distinguished false-positive samples from MGIT-positive samples, probably containing non-tuberculous mycobacteria. Thus, MPI shows promise in early TB diagnosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Magnetophoretic immunoassay (MPI) was developed to quantify <I>Mycobacterium tuberculosis</I> (MTB)-specific biomarker. </LI> <LI> The MPI offers a pico-molar level sensitivity with high specificity. </LI> <LI> Diagnostic utility of MPI assay was compared with that of the mycobacteria growth indicator tube (MGIT) test. </LI> <LI> This platform allows that the earlier monitoring of MTB growth in conventionally undetectable time range. </LI> <LI> And lower false-positive result from non-tuberculous mycobacteria (NTM) growth. </LI> </UL> </P>

Effects of myeloid sirtuin 1 deficiency on hypothalamic neurogranin in mice fed a high-fat diet

Kim, Kyung Eun,Jeong, Eun Ae,Shin, Hyun Joo,Lee, Jong Youl,Choi, Eun Bee,An, Hyeong Seok,Park, Kyung-Ah,Jin, Zhen,Lee, Dong Kun,Horvath, Tamas L.,Roh, Gu Seob Elsevier 2019 Biochemical and biophysical research communication Vol.508 No.1

<P><B>Abstract</B></P> <P>Hypothalamic inflammation has been known as a contributor to high-fat diet (HFD)-induced insulin resistance and obesity. Myeloid-specific sirtuin 1 (SIRT1) deletion aggravates insulin resistance and hypothalamic inflammation in HFD-fed mice. Neurogranin, a calmodulin-binding protein, is expressed in the hypothalamus. However, the effects of myeloid SIRT1 deletion on hypothalamic neurogranin has not been fully clarified. To investigate the effect of myeloid SIRT1 deletion on food intake and hypothalamic neurogranin expression, mice were fed a HFD for 20 weeks. Myeloid SIRT1 knockout (KO) mice exhibited higher food intake, weight gain, and lower expression of anorexigenic proopiomelanocortin in the arcuate nucleus than WT mice. In particular, KO mice had lower ventromedial hypothalamus (VMH)-specific neurogranin expression. However, SIRT1 deletion reduced HFD-induced hypothalamic neurogranin. Furthermore, hypothalamic phosphorylated AMPK and parvalbumin protein levels were also lower in HFD-fed KO mice than in HFD-fed WT mice. Thus, these findings suggest that myeloid SIRT1 deletion affects food intake through VMH-specific neurogranin-mediated AMPK signaling and hypothalamic inflammation in mice fed a HFD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Myeloid surtuin1 deficiency increases food intake and body weight in normal diet (ND)-fed mice. </LI> <LI> Myeloid surtuin1 deficiency decreases hypothalamic POMC and neurogranin in ND-fed mice. </LI> <LI> High-fat diet (HFD) increases hypothalamic neurogranin in mice. </LI> <LI> Myeloid surtuin1 deficiency reduces HFD-induced neurogranin, p-AMPK, and parvalbumin in the hypothalamus. </LI> </UL> </P>

Volumetric Analysis of Subcortical Structures in Obese at 3T

Eun-Bee Kim,Hyeon-Man Baek 한국자기학회 2019 Journal of Magnetics Vol.24 No.2

Obesity usually occurs due to homeostasis and hedonic food intake behavior caused by plasticity variation in both cortical and subcortical brain structures. However, little volumetric analysis has been done to study the relationships between obesity and subcortical structures. For this study, we aimed to investigate the volumetric differences of subcortical structures between 21 obese patients and 10 healthy controls using high resolution 3T MRI T1-weighted scans. Obese patients showed reduced subcortical gray matter volume in right caudate and right nucleus accumbens and enlarged volume in right amygdala. Vertex-wise shape analysis of subcortical structures showed bilateral caudate alterations in obese patients. Moreover, the bilateral amygdala negatively correlated with increasing age in obese patients. In conclusion, we present data showing association between obesity and subcortical brain structures. Various studies have shown that morphological changes can cause functional modifications in the brain. Therefore, we believe our analysis of volumetric differences in subcortical structures could be helpful for identifying neurophysiological changes that occur in obese patients.

<i>In vivo</i> synthesis of europium selenide nanoparticles and related cytotoxicity evaluation of human cells

Kim, Eun Bee,Seo, Ji Min,Kim, Gi Wook,Lee, Sang Yup,Park, Tae Jung Elsevier 2016 Enzyme and microbial technology Vol.95 No.-

<P>Nanotechnology strives to combine new materials for development of noble nanoparticles. As the nanoparticles exhibit unique optical, electronic, and magnetic properties depending on their composition, developing safe, cost-effective and environmentally friendly technologies for the synthesis have become an important issue. In this study, in vivo synthesis of europium selenide (EuSe) nanoparticles was performed using recombinant Escherichia coli cells expressing heavy-metal binding proteins, phytochelatin synthase and metallothionein. The formation of EuSe nanoparticles was confirmed by using UV-vis spectroscopy, spectrofluorometry, X-ray diffraction, energy dispersive X-ray and transmission electron microscopy. The synthesized EuSe nanoparticles exhibited high fluorescence intensities as well as strong magnetic properties. Furthermore, anti-cancer effect of EuSe nanoparticles against cancer cell lines was investigated. This strategy for the biogenic synthesis of nanoparticles has a great potential as bioimaging tools and drug carrying agents in biomedical fields due to its simplicity and nontoxicity. (C) 2016 Elsevier Inc. All rights reserved.</P>

An Educational Study on the Failure Process of Re󰠏employment of University Graduates with Resignation Experience

Eun Bee Kim(김은비) 중앙대학교 한국인적자원개발전략연구소 2018 역량개발학습연구 Vol.13 No.3

본 연구는 퇴사경험이 있으면서 재취업에 실패한 대졸 청년들의 재취업 실패과정의 구조를 분석하여, 청년의 재취업 실패과정에 관해 설명, 해석, 예측 및 적용이 가능한 이론을 제시하고자 하였다. 이때, 이론적·개념적 표집 방법을 사용하여 모집된 11명의 연구 참여자들을 대상으로 심층면접을 실시하였다. 이렇게 수집된 연구 자료들은 근거이론 분석방법으로 이론적 표본 추출과정을 거쳐 분석하며 연구를 진행하였다. 함께 총 3단계의 분석과정을 통해 점차 추상성을 높이는 방식으로 진행하였다. 본 연구의 결과는 다음과 같다. 첫째, 개방코딩 과정에서 최종 150개의 개념, 38개의 하위범주, 12개의 범주가 도출되었다. 다음으로, 축코딩의 결과, 퇴사·재취업 청년의 재취업 실패과정 패러다임 모형을 제시하였고 과정분석을 통해 퇴사·재취업 청년의 재취업 실패과정 단계를 제시하였다. 분석의 마지막 단계인 선택코딩 과정에서는, 본 연구의 중심이 되는 ‘재취업 실패 청년들의 자기직면 : 직업선택 시 기준의 극단성’의 핵심범주를 발견하고, 이후 이야기 윤곽을 통해서 선택코딩의 결과에 대한 이해를 돕고자 하였다. 또한 연구를 통해 도출된 퇴사경험이 있는 대졸 청년의 재취업 실패과정 모형으로 ‘재취업 실패 청년들의 자기직면 : 직업 선택 시 기준의 극단성’으로 이론을 제시하였다. 본 연구는 그 결과를 바탕으로 퇴사경험이 있는 대졸 청년의 반복적인 재취업 실패과정을 해석하고 이해 할 수 있도록 교육학적인 관점으로 실제적인 근거를 마련하고, 배경이 되는 맥락적 요소들이 퇴사·재취업 청년들의 재취업 실패과정에 미치는 영향력을 분석하였다. 다음으로 청년실업이라는 문제에 비해 소외되었던 재취업 실패 과정에 있는 퇴사·재취업 청년들의 이야기를 통해, 그들의 경험과 사례를 근거로 반복적인 재취업 실패과정의 이론이 도출되었다. This study analyzed the structure of failure in reemployment of young university graduates with experience of leaving a company, and based on the experience, suggested an applicable substance theory possible to explain, interpret, and predict the process of failure. According to the result, first, a total of 150 concepts, 38 subcategories, and 12 categories were deducted in the process of open coding. Second, a paradigm model of young university graduates with experience of leaving a company as a result of axial coding and the stage of failure in reemployment through the process analysis were suggested. A central phenomenon of the paradigm model turned out to be repetitive failures in reemployment. Especially, negative awareness on experience in leaving a company and failure in reemployment due to a lack of preparation led to learned lethargy, and under such situations and context conditions such as the severance of human relationships, financial difficulties and low self-esteem were deducted. In this process, facing reality, grasping the situation, and exploring the necessary and satisfying factors for reemployment played as an interventional condition, and a central phenomenon called repetitive failures in reemployment was deducted. Interaction strategies appeared as finding incomplete occupational values, exploring one’s own competitiveness in the labor market and others; and such behaviors led to extreme career choice standards, inflexible career choice values, etc.