토당귀 종자의 형태적 특성과 발아와 입모율에 대한 저장방법,생장조절제 및 프라이밍 처리 효과

안희정,안복주,·김도현,·안영섭,·김영국,박춘근,이상원,·박충범·차선우,·송범헌, 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was conducted to have basic and applied informations to establish the cultivation method and to improve the cultivation techniques of Angelica gigas Nakai through investigating the rates of germination and seedling establishment with treatment of plant growth regulator and seed priming. The germination rates of round shape seed were about 0.3~12% higher than those of parallel shape. The germination rates with different storage durations were increased at the level of 25oC and 4oC with the storage days, while they were clearly higher at -20oC compared to those at 4oC and 25oC without the storage durations. With the results of germination rates with treating of plant growth regulator and seed priming, their rates with the round shape seeds were comparatively higher about 6% than those with parallel shape seed and they were increased with increasing of the storage durations. The germination rates were generally appeared higher with GA3 than did those with seed priming. The highest germination rate was appeared at 50 ppm GA3 of plant growth regulator and at -0.5 MPa PEG6000 of seed priming. The germination rates?ㅤ ?ㅤ of Angelica gigas were different with different temperature, storage durations and the treatments of plant growth regulator and seed priming. These results could be used according to different cultivating conditions.

유기질비료 시비량에 따른 오미자 묘목의 초기 생육특성에 관한 연구

이경아,박태호,박성용,김도현,오문국,김민수,안태진,이상원,김영국,안영섭,박충범,차선우,송범헌 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was carried out to have basic and applied informations to find the optimum nitrogen fertilization level for organic cultivation and to investigate early growth characteristics of Schizandra chinensis Baillon. Different application levels of the organic fertilizer were based on the recommended rate of N fertilizer. Five different levels of the organic fertilizer were applied to S. chinensis and early growth responses such as the length of vine, the diameter of vine, the number of leaves, and the number of branches were investigated. Lengths of main and total vines were shown longer at the level of 1.0 time organic fertilizer than those at others. The vine length at early growth was the highest at the level of 1.0 time organic fertilizer among the treatments. Stem diameter was shown thicker level of 1.0 time organic fertilizer than others on August and September, whereas on October it was the thickest in the conventional cultivation. Number of leaf was increased higher at the level of 1.0 and 2.0 times organic fertilizer than that in the compared to conventional. The number of branches was appeared the lowest at the level of 1.0 time organic fertilizer at early growth, however they were increased through progressing their growth stages. They were not significantly difference among the treatments except for the level of 2.0 times organic fertilizer. Based on the research results, the growth of S. chinensis was commonly higher at the level of 1.0 time organic fertilizer compared to other treatments.

남자 알코올 의존 환자의 기질적 특성에 관한 연구

김성곤,민영기,이덕기,김미영,송영상,권도훈,고영훈,박제민,정병선 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

목 적: 본 연구의 목적은 한국인 남자 알코올 의존 환자의 기질적 특성을 Temperament and Character Inventory(TCI)를 이용하여 조사하는 것이다. 방 법: DSM-Ⅳ 진단 기준에 맞는 알코올 의존 환자 30명과 정상인 36명을 대상으로 연구학적 특성을 조사하고, 기질적 특성을 한글판 TCI를 이용하여 조사하였다. 모든 대상자의 나이는 30~60세였다. 결 과: 1) 두 군간 평균 나이는 유의한 차이가 없었으나, 교육 정도와 결혼을 유지하고 있는 비율 및 취업률은 알코올 의존군이 정상 대조군에 비해 유의하게 낮았다. 2) 알코올 의존군에서는 정상 대조군에 비해 Harm Avoidance(HA) 점수와 Self-transcendence(ST)점수는 유의하게 높았으며, Self-directiveness(SD)점수는 유의하게 낮았다. 3) 알코올 의존군 내에서의 각 척도들 간의 상관관계는, HA와 SD 점수, HA와 Persistence(PE) 점수, ST와 SD 점수는 각각 유의한 음의 상관관계, ST와 PE 점수, SD 점수와 Cooperativeness(CO) 점수, Reward Dependence(RD)와 CO 점수는 양의 상관관계를 보였다. 결 론: 이러한 본 연구의 결과는 한국인 남성에서 수줍음을 잘 타며, 걱정도 많고, 염세적이고 쉽게 피로해지는 기질적 특성이 알코올 의존과 연관성이 있음을 의미한다. Objectives : The purpose of this study was to assess the temperament of male parients with alcohol dependence. Methods : The subjects were 30 patients who met DSM-Ⅳ criteria for alcohol dependence and 36 normal controls. All subjects completed Temperament and Character Inventory(TCI) which was developed by Cloninger. Results : 1) The educational level, marital status, and occupation in alcohol dependence group were significantly lower than those of normal control group, but there was no difference in the age between two groups. 2) Among the scores of TCI, the score of harm avoidance(HA) and self-transcendence(ST) were significantly higher in the alcohol dependence group than in the normal control group. 3) In the alcohol dependence group, a significant negative correlation among the scores of TCI was between HA and SD, HA and Persistence(PE), ST and SD, and positive correlation between ST and PE, SD and Cooperativeness(CO), Reward Dependence(RD) and CO. Conclusion : These results suggest that shy, fearful, pessimistic and fatigable temperament are related to developing alcohol dependence in Korean males.

HCV : PE-099 ; Establishment of an in vivo model of hepatocellular carcinoma using SV40T antigen transgenic mice

( Do Seon Song ),( Hee Yeon Kim ),( Myung Jun Song ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Transgenic mouse model can be helpful in predicting the clinical response of hepatocellular carcinoma (HCC). But it has a drawback in visualizing endogenously formed tumors. The aim of this study was to establish a transgenic mouse model using SV40 large T-antigen (Tag), and to evaluate the usefulness as non-invasive tools of magnetic resonance imaging (MRI) for HCC. Methods: We have generated transgenic mouse containing the oncogene SV40 large T agtigen under the control of liver specific albumin promoter/α-fetoprotein enhancer. The resulting transgenic mice are predisposed to inheritable liver tumor development. When HCC was verified grossly, mice underwent T2-weighted MR imaging examination with 4.7-T(Tesla) MR scanner. After 4 weeks of epirubicin based chemotherapy, mice underwent 2nd MRI examination and those images were correlated with the pathologic examination. Results: HCCs were identified at 8 months of age in 1 mouse and 10 months of age in 3 mice, grossly. Grossly, In the T2-weighted MR imaging, tumor exhibited slightly high signal intensity (No.1: 10.2x8.2 mm, No.2: 10.3x5.7 mm, No.3 : 19.3x15.7mm, No.4: 14.2x10.1 mm and 11.5x8.6 mm). After 4 weeks of chemotherapy, complete response was observed in No.2, partial response in No.3 and No.4, and progressive disease in No.1 in T2-weighted MR images. Pathologic examination showed Edmondson-Steiner grade III HCC in No.1, focal grade II HCC in No.2, grade II in No.3 and grade II in No.4. Conclusions: We established transgenic mouse model expressing oncogen SV40 Tag, and those induced in vivo HCCs. In addition, T2-weighted MR imaging can reveal the hepatic tumors in a live murine model. Thus, SV40 Tag expressing transgenic mice could be effective in vivo model for HCC, and T2 weighted MR imaging could be helpful in detection of hepatic tumors and evaluation of response in transgenic mice model.

Association between Sarcopenia and Minimal Hepatic Encephalopathy and Quality of Life in Patients with Liver Cirrhosis

( Do Seon Song ),( Dae Won Jun ),( Jae Yoon Jeong ),( Tae Yeob Kim ),( Sang Bong Ahn ),( Hee Yeon Kim ),( Young Kul Jung ),( Myeong Jun Song ),( Sung Eun Kim ),( Hyoung Su Kim ),( Soung Won Jeong ),( 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: We aimed to investigate the association between sarcopenia and minimal hepatic encephalopathy and quality of life in cirrhotic patients. Methods: Liver cirrhosis patients associated with chronic hepatitis B were prospectively recruited in 15 hospitals between April 2016 and December 2017. Among these 223 patients, 128 patients who assessed handgrip strength and skeletal muscle mass were analyzed. Minimal hepatic encephalopathy was diagnosed when psychometric hepatic encephalopathy score (PHES) was less than -4. Quality of life was assessed by 36-Item Short Form Health Survey (SF-36). Sarcopenia was defined as lower quintile using handgrip strength and skeletal muscle index (SMI). SMI was calculated by total muscle mass/height². Results: Mean age was 53.5 years, and 63.3% was male. MHE was 27.3%. Cut-off values of sarcopenia were as follows; 10.2 kg/m2 in men and 8.15kg/m2 in women for sarcopenia by SMI, and 27 kg in men and 18 kg in women for sarcopenia by handgrip strength. There were no significant differences in the prevalence of MHE and PHES score value between sarcopenic and non-sarcopenic group (P=0.077 and P=0.152 by SMI, P=0.935 and P=0.888 by handgrip strength). An SF-36 score of the sarcopenic group was significantly lower than that of the non-sarcopenic group (60.2±17.9 vs. 68.1±17.1, P=0.038) by SMI, but not by handgrip strength (62.6±16.8 vs. 67.3±17.7, P=0.260). Among 8 domains constituting SF-36 score, general health score was significantly lower in the sarcopenic group by SMI (P=0.032). Pain score and energy/fatigue scores were significantly lower in the sarcopenic group by handgrip strength (P=0.035 and P=0.020). Conclusions: Sarcopenia by SMI and handgrip strength was not associated with MHE. However, sarcopenia by SMI and handgrip strength were significantly associated with quality of life.

HCC : PE-065 ; Prognostic factors of hepatic arterial infusion chemotherapy with advanced hepatocellular carcinoma with portal vein thrombosis

( Do Seon Song ),( Hee Yeon Kim ),( Myung Jun Song ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Hepatic arterial infusion chemotherapy(HAIC) has been reported to be effective in patients with advanced hepatocellular carcinoma (HCC). In this study, we aimed to investigate the prognostic factors of HAIC. Methods: Between March 2009 and December 2011, we invastigated clinical data of 56 patients with advanced HCC who received over 2 cycles of HAIC in Seoul St. Mary hospital. The patients were underwent HAIC via implantable port system. Demographic variables, laboratory values, and tumor characteristics were determined retrospectively. Treatment response were examined by modified Response Evaluation Criteria for Solid Tumors criteria. Results: The median age of the patients was 54(36-74) years and 46 patients (82%) were male. A total of 326 cycles of HAIC was given to the 56 patients, with median of 5 cycles(2-25 cycles). The etiology of liver disease were hepatitis B in 43 patients (77%), hepatitis C in 5 (9%) and non-viral causes in 8 (14%). Twenty-seven patients (47%) received combination treatment with other modalities. The best treatment response during HAIC were complete response (CR) in 4 (7%) patients, partial response (PR) in 15 (27%), stable disease (SD) in 30 (54%), and progressive disease (PD) in 7 (13%). Overall survival (OS) was median 8 months (2-31). On multivariate analysis, intrahepatic tumor control (CR, PR, or SD)(p=0.043, OR=32.4), alpha-fetoprotein(AFP) response (reduction of AFP more than 50%) (p=0.016, OR=64.1), and tumor response after 2nd cycle HAIC(p=0.008, OR=356.7) were significant predictive factors for CR or PR during HAIC. Better survival was associated with best tumor response during HAIC (p<0.001, OR=8.98), Low AFP level(<100 ng/mL) (p=0.006, OR=3.82), and small tumor volume(total tumor volume/total liver volume<0.2)(p=0.02, OR=2.35). Portal vein thrombosis (p=0.273) and extrahepatic metastasis(p=0.259) were not associated significantly. Conclusions: HAIC can be good treatment modality for patients with low AFP level and small proportion of tumor. In addition, well controlled intrahepatic HCC is important prognostic factor in HAIC treatment.

HCC : Prognostic Factors of Hepatic Arterial Infusion Chemotherapy in Advanced Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis

( Do Seon Song ),( Si Hyun Bae ),( Myeong Jun Song ),( Sung Won Lee ),( Hee Yeon Kim ),( Young Joon Lee ),( Jung Suk Oh ),( Ho Jong Chun ),( Hae Giu Lee ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Hepatic arterial infusion chemotherapy (HAIC) is used in advanced Hepatocellular carcinoma (HCC) patients with portal vein tumor thrombosis (PVTT). The objective of this study was to evaluate the prognostic factors and anti-tumor effects of HAIC in HCC with PVTT. Methods: Fifty HCC patients with PVTT were treated by HAIC via a subcutaneously implanted port. The ECF chemotherapeutic regimen consisted of 35 mg/m2 epirubicin on day 1, 60 mg/m2 cisplatin for 2 h on day 2, and 500 mg/m2 5-fluorouracil for 5 hours on days 1-3. Treatments were repeated every 3 or 4 weeks. Results: Three (6%) of the 50 patients achieved a complete response (CR), 13 (26%) showed partial response (PR), and 22 (44%) had stable disease (SD). The median survival and time to progression were 7 months and 2 months, respectively. Signifi- cant pre-treatment prognostic factors were a tumor volume of <400 cm3 (P=0.007) and normal levels of protein induced by vitamin K absence or antagonist (PIVKA)-II (P=0.016). After two cycles of treatment, disease control (CR+PR+SD) and PIVKA-II reduction of over 50% were independent factors for survival (P=0.048 and P=0.025). Objective response (CR+PR) and PVTT response were also significant prognostic factors (P=0.043 and P=0.005). Conclusions: HAIC may therefore be considered as an effective treatment modality for advanced HCC with PVTT in patients with good prognostic factors.

A comparative study between sorafenib and hepatic arterial infusion chemotherapy for advanced hepatocellular carcinoma with portal vein tumor thrombosis

Song, Do Seon,Song, Myeong Jun,Bae, Si Hyun,Chung, Woo Jin,Jang, Jae Young,Kim, Young Seok,Lee, Sae Hwan,Park, Jun Yong,Yim, Hyung Joon,Cho, Sung Bum,Park, Soo Young,Yang, Jin Mo Springer-Verlag 2015 Journal of gastroenterology Vol.50 No.4

Interferon-Free Treatment of Chronic Hepatitis C Patients with Decompensated Liver Cirrhosis in a Real-Life Cohort

( Do Seon Song ),( U Im Chang ),( Jin Mo Yang ),( Hee Yeon Kim ),( Chang Wook Kim ),( Chan Ran You ),( Sang Wook Choi ),( Se Hyun Cho ),( Joon-Yeol Han ),( Sung Won Lee ),( Hae Lim Lee ),( Nam Ik Han 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: We investigated the effectiveness and tolerability of interferon- free regimen in patients with chronic hepatitis C virus (HCV) infection and liver decompensation. Methods: The study included 40 advanced liver disease patients who were infected with HCV and started treatment with interferon free regimen between October 2015 and January 2017 at our liver unit. Advanced liver disease was defined as cirrhosis with Child-Pugh score over 7 or evidence of current or previous decompensation. Results: Median age was 57.5 years, and 22 patients (55%) were male. Child-Pugh class B and C patients were 30 and 5, respectively. Of the treated patients, 26 (65%) had genotype 1b, 13 (32.5%) genotype 2, and one (2.5%) genotype 3a. Eleven (27.5%) were previously pegylated interferon-experienced patients. In genotype 1b patients, daclatavir(DCV)+asunaprevir(ASV) was the most used regimen (57.7%), followed by sofosbuvir(SOF)+ledipasvir(LDV)+ribavirin(RBV) (34.6%) and SOF+DCV+RBV (7.7%). In genotype 2a patients, all patients treated with SOF+RBV, and one patient with genotype 3a was treated with SOF+DCV. In total, 33 (89.2%) completed treatment, 2 (5.4%) lost to follow-up, one (2.7%) died due to variceal bleeding, and the remaining one (2.7%) stopped treatment due to adverse event. Rapid virologic response rate was 83.8%, end-of-treatment response rate was 86.5%, and sustained virologic response rate at 12 week was 77.3%. Mean Model for End-stage Liver Disease (MELD) score was significantly decreased (11.86±3.43 vs. 11.28±3.87, P<0.001). Most common adverse event was jaundice (56.8%), followed by anemia (40.5%), aminotranferase elevation (40.5%). Treatment completion failure rates were 0%, 7.1%, and 33.3% in Child-Pugh class A, B, and C, respectively. Conclusions: In a real-life setting, interferon-free regimen is associated with high efficacy and an acceptable safety profile and improves liver function in the majority of decompensated liver cirrhosis patients with HCV infection. However, decompensated patients with Child-Pugh class C should be treated with caution due to low rates of treatment completion.

Outcome of transarterial chemoembolization-based multi-modal treatment in patients with unresectable hepatocellular carcinoma.

Song, Do Seon,Nam, Soon Woo,Bae, Si Hyun,Kim, Jin Dong,Jang, Jeong Won,Song, Myeong Jun,Lee, Sung Won,Kim, Hee Yeon,Lee, Young Joon,Chun, Ho Jong,You, Young Kyoung,Choi, Jong Young,Yoon, Seung Kew WJG Press 2015 WORLD JOURNAL OF GASTROENTEROLOGY Vol.21 No.8

<P>To investigate the efficacy and safety of transarterial chemoembolization (TACE)-based multimodal treatment in patients with large hepatocellular carcinoma (HCC).</P>