http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Dain Kim ),( Hyeseon Yoon ),( Sangkyu Kim ),( Jimin Wi ),( Heesu Chae ),( Gyunghee Jo ),( Jun-yeol Yoon ),( Heeyoun Kim ),( Chankyu Lee ),( Se-ho Kim ),( Hyo Jeong Hong ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
Cross-reactive material 197 (CRM<sub>197</sub>) is a non-toxic mutant of diphtheria toxin containing a single amino acid substitution of glycine 52 with glutamic acid. CRM<sub>197</sub> has been used as a carrier protein for poorly immunogenic polysaccharide antigens to improve immune responses. In this study, to develop a sandwich ELISA that can detect CRM<sub>197</sub> and CRM<sub>197</sub> conjugate vaccines, we generated a human anti-CRM<sub>197</sub> monoclonal antibody (mAb) 3F9 using a phage-displayed human synthetic Fab library and produced mouse anti-CRM197 polyclonal antibody. The affinity (K<sub>D</sub>) of 3F9 for CRM<sub>197</sub> was 3.55 nM, based on Bio-Layer interferometry, and it bound specifically to the B fragment of CRM<sub>197</sub>. The sandwich ELISA was carried out using 3F9 as a capture antibody and the mouse polyclonal antibody as a detection antibody. The detection limit of the sandwich ELISA was <1 ng/ml CRM<sub>197</sub>. In addition, the 3F9 antibody bound to the CRM<sub>197</sub>-polysaccharide conjugates tested in a dose-dependent manner. This ELISA system will be useful for the quantification and characterization of CRM<sub>197</sub> and CRM<sub>197</sub> conjugate vaccines. To our knowledge, this study is the first to generate a human monoclonal antibody against CRM197 and to develop a sandwich ELISA for CRM<sub>197</sub> conjugate vaccines.
Thermoelectric properties of solution-processed antimony-doped tin oxide thin films
Kim, Dain,Park, Juyun,Kim, Joo Hyun,Kang, Yong-Cheol,Kim, Hyun Sung Elsevier 2018 THIN SOLID FILMS - Vol.646 No.-
<P><B>Abstract</B></P> <P>Antimony (Sb)-doped tin oxide (ATO) nanoparticles with various doping amounts were prepared by solvothermal synthesis. ATO thin films were fabricated on glass substrates via spin coating using a prepared ATO nanoparticle suspension solution. The effect of doping amount and annealing temperature in production of thin film on Seebeck coefficient and electrical conductivity was investigated. The optimized doping conditions (2mol% Sb and annealing temperature=500°C) produced ATO thin films exhibiting power factors (S<SUP>2</SUP> σ), 64.4μWm<SUP>−1</SUP> K<SUP>−2</SUP> which is encouraging for synthesis using inexpensive and facile solution-processing procedures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Antimony doped tin oxide nanoparticles were synthesized by solvothermal reaction. </LI> <LI> Nanoparticle suspensions were employed for fabrication of thin film. </LI> <LI> The effect of Sb doping amount on thermoelectric performance was studied. </LI> <LI> The effect of annealing temperature on thermoelectric performance was studied. </LI> </UL> </P>
마이크로웨이브 진공건조로 생산된 마늘, 고추 분말의 품질특성
김다인(Dain Kim),여성순(Seoungsoon Yeo),신의철(Euicheol Shin),송혁환(Hyukhwan Song),김기화(Kihwa Kim),이형용(Hyoungyong Lee),정윤화(Yoonhwa Jeong),김미숙(Misook Kim),이영승(Youngseung Lee) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.10
이 연구는 열풍, 동결, 마이크로웨이브 진공 건조방법으로 생산한 마늘, 고추 분말의 품질을 비교하고 특성을 알아보는 것이 목적이었다. 일반성분에서는 건조방법에 따른 유의적인 차이를 보이지 않았다. 열풍 건조한 마늘, 고추 분말의 pH가 유의적으로 낮은 값을 보였고 동결건조 한 마늘, 고추분말의 용해도가 유의적으로 높은 값을 보였고 원물의 색상을 가장 잘 나타냈다. 수용성비타민은 마늘, 고추 분말 모두 열풍건조에서 비타민 B₁, B₂의 값이 유의적으로 낮게 나왔고 마이크로웨이브 진공건조에서 비타민 B₃, C의 값이 유의적으로 낮게 나타났다. 마늘 분말은 건조방법에 따른 무기질 함량의 차이가 없었으나 고추 분말은 동결건조 분말의 P, Mg, Mn, Cu, Zn 값이 유의적으로 낮게 나타났다. 물성은 마늘 분말에서는 열풍건조 분말의 응집성이 유의적으로 높은 값을 보였고 고추 분말에서는 마이크로웨이브 진공건조 분말의 응집성이 유의적으로 높게 나타났다. 소비자 조사결과 건조방법에 따른 분말의 외관 및 향 강도의 차이는 나타났으나 분말을 조리식품에 적용했을 때에는 소비자 기호도에 영향을 미치지 않았다. This study was conducted to compare and characterize the quality of garlic and chili pepper powders prepared by hot air drying (HD), freezing drying (FD), and microwave vacuum drying (MVD) methods. There was no significant difference in proximate analysis according to drying methods. The pH of HD dried garlic and chili pepper powder was 6.30 and 5.02 respectively, which were significantly lower than that of the MVD. The solubility of garlic powder was highest for FD, while the FD and MVD showed higher solubility of chill pepper powder. Both samples showed significantly lower values of vitamin B₁ and B₂ for HD and significantly lower values of vitamin C for MVD. There was no difference in the mineral contents of garlic powder according to the drying methods and the P, Mg, Mn, Cu, and Zn values of chili pepper powder were lowest for FD. The cohesiveness of HD powder was significantly higher in garlic powder, while the cohesiveness of MVD powder was significantly higher in chili pepper powder. Sensory evaluation showed no significant difference in characteristics when dry powder was applied to cooked food.
Photocatalytic Effect of Ag/TiO<sub>2</sub> Nanotubes Fabricated Using 40 nm-Scale BCP Lithography
Sung, DaIN,Kim, Hyun Gu,Cha, Seung Keun,Kim, Dong Hyun,Lee, Han-Bo-Ram,Kim, Sang Ouk,Kim, Dong Woo,Yeom, Geun Young American Scientific Publishers 2017 Nanoscience and Nanotechnology Letters Vol.9 No.1
Temporal Transcriptome Analysis of SARS-CoV-2-Infected Lung and Spleen in Human ACE2-Transgenic Mice
Jung Ah Kim,Sung-Hee Kim,Jung Seon Seo,노현아,Haengdueng Jeong,Jiseon Kim,Donghun Jeon,Jeong Jin Kim,Dain On,윤서연,Sang Gyu Lee,이윤우,Hui Jeong Jang,박인호,Jooyeon Oh,Sang-Hyuk Seok,Yu Jin Lee,홍승민,안세희,Joon-Yong 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.12
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.
Temporal Transcriptome Analysis of SARS-CoV-2-Infected Lung and Spleen in Human ACE2-Transgenic Mice
Jung Ah Kim,Sung-Hee Kim,Jung Seon Seo,노현아,Haengdueng Jeong,Jiseon Kim,Donghun Jeon,Jeong Jin Kim,Dain On,윤서연,Sang Gyu Lee,이윤우,Hui Jeong Jang,In Ho Park,Jooyeon Oh,Sang-Hyuk Seok,Yu Jin Lee,홍승민,안세희,Jo 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.12
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.
Immune Cells Are Differentially Affected by SARS-CoV-2 Viral Loads in K18-hACE2 Mice
Jung Ah Kim,Sung-Hee Kim,Jeong Jin Kim,Hyuna Noh,Su-bin Lee,Haengdueng Jeong,Jiseon Kim,Donghun Jeon,Jung Seon Seo,Dain On,Suhyeon Yoon,Sang Gyu Lee,Youn Woo Lee,Hui Jeong Jang,In Ho Park,Jooyeon Oh,S The Korean Association of Immunobiologists 2024 Immune Network Vol.24 No.2
Viral load and the duration of viral shedding of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are important determinants of the transmission of coronavirus disease 2019. In this study, we examined the effects of viral doses on the lung and spleen of K18-hACE2 transgenic mice by temporal histological and transcriptional analyses. Approximately, 1×10<sup>5</sup> plaque-forming units (PFU) of SARS-CoV-2 induced strong host responses in the lungs from 2 days post inoculation (dpi) which did not recover until the mice died, whereas responses to the virus were obvious at 5 days, recovering to the basal state by 14 dpi at 1×10<sup>2</sup> PFU. Further, flow cytometry showed that number of CD8+ T cells continuously increased in 1×10<sup>2</sup> PFU-virus-infected lungs from 2 dpi, but not in 1×10<sup>5</sup> PFU-virus-infected lungs. In spleens, responses to the virus were prominent from 2 dpi, and number of B cells was significantly decreased at 1×10<sup>5</sup> PFU; however, 1×1<sup>2</sup> PFU of virus induced very weak responses from 2 dpi which recovered by 10 dpi. Although the defense responses returned to normal and the mice survived, lung histology showed evidence of fibrosis, suggesting sequelae of SARS-CoV-2 infection. Our findings indicate that specific effectors of the immune response in the lung and spleen were either increased or depleted in response to doses of SARS-CoV-2. This study demonstrated that the response of local and systemic immune effectors to a viral infection varies with viral dose, which either exacerbates the severity of the infection or accelerates its elimination.
Kyung-Hee Kim,Sanghyun Lee,Hyo Sun Jung,Julan Kim,Jong-Won Park,Choul-Ji Park,Hyejin Kim,Woo-Jin Kim,Dain Lee 한국발생생물학회 2020 발생과 생식 Vol.24 No.3
The caspase10 encodes an initiating caspase that plays an important role in the maintaining the cellular homeostasis by regulating the steps involved in the immune response and cell death. We investigated the expression of caspase10 during the different developmental stages and in olive flounder tissues. Caspase10 increased in the late stage of the formation of immune tissue, and high expression was observed in the gills, kidney, skin, and spleen. The current study analyzed the expressional changes of caspase10 in olive flounder infected with viral hemorrhagic septicemia virus (VHSV). One of the major causes of mass mortality, VHSV infection in olive flounder attributes to significant expression of caspase10 in the gills, spleen, skin, and kidneys. The results indicate a close association of caspase10 expression with the immune response to VHSV infection in olive flounder. The observations could form the basis data for exploration of other fish immune system.