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( Geun Hyoung Choi ),( Leesun Kim ),( Deuk Yeong Lee ),( Cho Long Jin ),( Sung Jin Lim ),( Byung Jun Park ),( Nam Jun Cho ),( Jin Hyo Kim ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.2
The quantitative analytical method for the bioactive substance, 3-cyano-4-methoxy-N-methyl-2-pyridone (ricinine) and an index compound, ricinoleic acid in castor plant (Ricinus communis) extract or oil was developed. For the determination of a pyridone alkaloid compound, ricinine, successive cartridge cleanup method combined with ultra-performance liquid chromatography was set up with ENVI-CarbTM (0.5 g) and C18 SPE cartridges. Accuracy and precision were evaluated through fortification studies of one biopesticide (PE) at 10 and 100 mg kg.1. Mean recoveries of ricinine were 98.7 and 96.0 % associated with less than 10 % RSD, respectively. For the determination of ricinoleic acid in castor extract and oil, saponification and methylation were optimized using gas chromatography-time of flight mass spectrometry. Recovery was more than 84.8 % associated with 6.2 % RSD after derivatization procedure. Both methodologies developed were applied to analyze real samples including three castor oil products and six commercially available biopesticides containing R. communis, collected at Korean market. The contents of ricinine and ricinoleic acid in most commercial biopesticides were less than the oil or extract contents indicated by label.
Cho, Yoon Shin,Chen, Chien-Hsiun,Hu, Cheng,Long, Jirong,Hee Ong, Rick Twee,Sim, Xueling,Takeuchi, Fumihiko,Wu, Ying,Go, Min Jin,Yamauchi, Toshimasa,Chang, Yi-Cheng,Kwak, Soo Heon,Ma, Ronald C W,Yamamo Nature Publishing Group, a division of Macmillan P 2012 Nature genetics Vol.44 No.1
We conducted a three-stage genetic study to identify susceptibility loci for type 2 diabetes (T2D) in east Asian populations. We followed our stage 1 meta-analysis of eight T2D genome-wide association studies (6,952 cases with T2D and 11,865 controls) with a stage 2 in silico replication analysis (5,843 cases and 4,574 controls) and a stage 3 de novo replication analysis (12,284 cases and 13,172 controls). The combined analysis identified eight new T2D loci reaching genome-wide significance, which mapped in or near GLIS3, PEPD, FITM2-R3HDML-HNF4A, KCNK16, MAEA, GCC1-PAX4, PSMD6 and ZFAND3. GLIS3, which is involved in pancreatic beta cell development and insulin gene expression, is known for its association with fasting glucose levels. The evidence of an association with T2D for PEPD and HNF4A has been shown in previous studies. KCNK16 may regulate glucose-dependent insulin secretion in the pancreas. These findings, derived from an east Asian population, provide new perspectives on the etiology of T2D.
Effect of Heat Treatment on CO<SUB>2</SUB> Adsorption of Ammonized Graphite Nanofibers
Long-Yue Meng,Ki-Sook Cho,Soo-Jin Park 한국탄소학회 2010 Carbon Letters Vol.11 No.1
In this work, graphite nanofibers (GNFs) were prepared by ammonia and heat treatment at temperatures up to 1000℃ to improve its CO2 adsorption capacity. The effects of the heat treatment on the textural properties and surface chemistry of the GNFs were investigated by N2 adsorption isotherms, XRD, and elemental analysis. We found that the chemical properties of GNFs were significantly changed after the ammonia treatment. Mainly amine groups were formed on the GNF surfaces such as lactam groups, pyrrole and pyridines. The GNFs treated at 500℃ showed highest CO2 adsorption capacity of 26.9 mg/g at 273 K in this system.
Cho, Jung-Keun,Rengasamy, Rajesh,Curtis-Long, Marcus John,Kim, Jin-Hyo,Lee, Ji-Won,Park, Ki-Hun The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Azasugars derived from L-alanine and L-serine were screened for inhibitory activity against ${\alpha}$-rhamnosidase. The enantiomers of 1,6-dideoxynojirimycin ($ent$-1,6-dDNJ) (1) and ($2S$,$3R$)-2-(hydroxymethyl)piperidin-3-ol (5) showed highly specific and potent inhibition against ${\alpha}$-rhamnosidase with $K_i$ values of 4.2 and $16.6{\mu}M$, respectively. Structure of the best inhibitor features the same stereochemical configuration as L-rhamnose at C2, C3, and C4 centers. In kinetic studies, both compounds exhibited competitive inhibition behavior. Compound 1 manifested simple reversible slow-binding inhibition with the following kinetic parameters: ${\kappa}_3=1.17nM^{-1}\;min^{-1}$, ${\kappa}_4=5.96{\times}10^{-3}min^{-1}$, and $K_i^{app}$=5.1 mM.
Evaluation of a novel bifunctional xylanase–cellulase constructed by gene fusion
An, Jin Mee,Kim, Young Kyun,Lim, Woo Jin,Hong, Su Young,An, Chang Long,Shin, Eun Chule,Cho, Kye Man,Choi, Byoung Rock,Kang, Jung Mi,Lee, Sun Mi,Kim, Hoon,Yun, Han Dae Elsevier 2005 Enzyme and microbial technology Vol.36 No.7
<P><B>Abstract</B></P><P>An artificial bifunctional enzyme, xylanase–cellulase, has been prepared by gene fusion. Three chimeric genes were constructed that encoded fusion proteins of different lengths. The fusion proteins exhibited both xylanase (XynX) and cellulase (Cel5Z::Ω) activity when <I>cel</I>5Z::Ω was fused downstream of <I>xyn</I>X, but not when <I>xyn</I>X was fused downstream of <I>cel</I>5Z::Ω. Activities of bifunctional enzymes decreased when a shorter xylanase peptide was fused. Three fusion enzymes were purified, and the molecular weights of the enzymes were estimated by CMC-SDS-PAGE and XYN-SDS-PAGE to be 149, 129, and 87kDa, respectively. The fusion enzymes displayed optimum cellulase activity at pH 8.0 and 50°C and optimum xylanase activity at pH 8.0 and 70°C.</P>
Guo, Long Zhe,Kim, Moo Hyun,Shin, Eun Seok,Ann, Soe Hee,De Jin, Cai,Cho, Young-Rak,Park, Jong Sung,Park, Kyungil,Park, Tae-Ho,Lee, Michael S.,Serebruany, Victor L. Elsevier 2016 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.222 No.-
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>The impact of thienopyridine reloading on clinical outcomes, and residual high platelet reactivity (HPR) is unclear. We sought to compare the HRP-related effect of prasugrel and clopidogrel reloading in the already clopidogrel-loaded patients undergoing percutaneous coronary intervention (PCI).</P> <P><B>Materials and methods</B></P> <P>In this prospective, two-center, randomized, open-label study, patients with HPR who had undergone PCI after a clopidogrel (300–600mg) loading dose (LD) were enrolled. Among screened (n=153), HPR was determined in seventy-six patients, who were randomized to either repeated clopidogrel (300mg LD, followed by 75mg MD daily) or prasugrel (20mg LD, followed by 5mg MD daily). The primary endpoint was HPR at 24h after PCI, as determined by the VerifyNow assay. The rates of sustained high and low platelet reactivity, periprocedural myocardial injury (PMI) and 30-day clinical outcomes were also assessed.</P> <P><B>Results</B></P> <P>Higher inhibition of platelet reactive units (PRU) was observed in the prasugrel group than after clopidogrel reloading (Pre-PCI: 284.4±32.0 vs 279.5±32.5, p=0.504; Post-PCI: 100.0±67.0 vs 202.9±65.8, p<0.001; 30days: 170.8±69.8 vs 215.1±62.4, p=0.007). There were less HRP post-PCI after prasugrel compared with the clopidogrel group (2.7 vs 36.1%, p<0.001). However, reloading with prasugrel did not reduce PMI compared to clopidogrel (36.8% vs 39.5%, p=0.813).</P> <P><B>Conclusion</B></P> <P>Prasugrel reloading led to a greater reduction in HPR, but similar with clopidogrel PMI in post-PCI patients. Larger randomized evidence is needed for optimization of loading strategies with thienopyridines.</P> <P>Clinical Trial Registration Information: NCT01609647.</P>