Crystal transformation during alkaline swelling of wood cellulose by particle size and heat-treatment

Ah Ran Kim,Nam Hun Kim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09

In this study, we examined the effect of particle size and heat treatment on the crystal transformation of wood cellulose during alkaline swelling. We used wood flours which have different size as 40mesh, 60mesh, 100mesh, 200mesh and wood chips from Paulownia tomentosa for the samples. Control and heat-treated samples at 100℃ and 200℃ were treated with 15% NaOH solution for 10 minutes, and followed by washing with distilled water and dried at room temperature. We observed the morphology of wood samples using an optical microscope, and compared the relative crystallinity and crystal width before and after alkali -treatment and heat-treatment using an X-ray diffraction method. During alkali swelling after heat-treatment, the crystal transformation of cellulose I to cellulose II was also investigated. By alkali treatment, the color of the wood samples tended to be dark, and the alkali-treated samples shrank more than the untreated samples. The relative crystallinity of wood cellulose decreased with decreasing particle size of wood flours and slightly increased with increasing temperature, and the crystal width was not significantly different. Also, heat-treated samples were easily transformed to cellulose II structure compared to control samples.

Sensory Based Feeding Intervention for Toddlers With Food Refusal: A Randomized Controlled Trial

Ah-Ran Kim,Jeong-Yi Kwon,Sook-Hee Yi,Eun-Hye Kim 대한재활의학회 2021 Annals of Rehabilitation Medicine Vol.45 No.5

Objective To investigate the effect of sensory-based feeding treatment for toddlers with food refusal compared with only providing nutrition education. Methods Thirty-two toddlers with food refusal were randomly assigned to an intervention group or the control group. Toddlers in the intervention group received the sensory-based feeding intervention and the duration was for 1 hour for 5 days per week for 4 weeks, and then 1 hour, once a week for 8 weeks. Subjects in both the intervention and control groups received nutritional education once every 4 weeks for 12 weeks. The participants were evaluated at their entry into the study and 12 weeks later based on height, weight, behavior at mealtime using the Behavioral Pediatrics Feeding Assessment Scale (BPFAS), and sensory processing ability using the Infant/Toddler Sensory Profile. Results Sixteen toddlers were included in each group. Two subjects in the intervention group and four toddlers in the control group were excluded from the final analysis. Significant improvements in child or parent subscales of the BPFAS were observed in the intervention group. In contrast, there were no significant improvements in any BPFAS scores in the control group. Conclusion Sensory-based feeding intervention was effective for improving mealtime behavior in toddlers with food refusal. Therefore, a sensory-based feeding intervention could be considered as an intervention approach to address feeding disorders in toddlers.

Direct potentiation of NK cell cytotoxicity by 8-azaguanine with potential antineoplastic activity

Kim, Nayoung,Choi, Ji-Wan,Song, Ah Young,Choi, Woo Seon,Park, Hye-Ran,Park, Sojung,Kim, Inki,Kim, Hun Sik Elsevier 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.67 No.-

<P><B>Abstract</B></P> <P>This study identified 8-azaguanine (8-AG) as a novel immunomodulatory drug (IMiD) through a high-throughput screen of the Preswick Chemical Library in a model of human NK cell cytotoxicity against blood cancer cells. 8-AG, originally developed as an antineoplastic agent, significantly increased the cytotoxicity of NK cells and was superior in this activity to previously known IMiDs, such as fluoxetine and amphotericin B, identified from the same library. IFN-γ expression was also slightly increased by 8-AG. Mechanistically, 8-AG increased conjugate formation between NK and target cells and subsequent cytolytic granule polarization, but not calcium mobilization, regulation of activating receptors, or expression of perforin or granzyme B. Thus, the antineoplastic activity of 8-AG should be re-evaluated in light of this novel potentiating effect on NK cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 8-azaguanine (8-AG) was originally developed as an antineoplastic agent. </LI> <LI> 8-AG was identified by high-throughput screen to increase the cytotoxicity of NK cells. </LI> <LI> 8-AG increased the formation of NK-target conjugates and cytolytic granule polarization. </LI> <LI> 8-AG serves a dual function as antineoplastic and immunomodulatory drug. </LI> </UL> </P>

Effect of delignification on crystal transformation of alkali-treated wood cellulose

Ah Ran Kim,Nam Hun Kim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09

This study was carried out to understand the effect of delignification on crystal transformation of alkali-treated wood cellulose by milling and heat-treatment. We used wood flours in different size with 40mesh, 60mesh, 100mesh, 200mesh and wood chips of Paulownia tomentosa for the samples. Control and heat treated samples at 100℃ and 200℃ were examined. The samples were treated with 15% NaOH solution for 10 minutes, and followed by washing with distilled water and dried at room temperature. Additionally, the alkali-treated wood samples were soaked in Schűltz solution for one day and heated at 60℃ for 1 hours, and then washed with distilled water until neutralization. The morphology of wood particles was observed using an optical microscope. Crystalline properties and crystal transformation from cellulose I to cellulose II of the samples before and after delignification were investigated by an X-ray diffraction method. In the X-ray diffraction analysis, it is revealed that the crystal transformation from cellulose I to cellulose II was proceeded easily by delignification. After delignification of alkali-treated samples, also, smaller particle size of wood samples, the more crystal transformation from cellulose I to cellulose II. Moreover, heat-treated samples were more easily transformed to cellulose II structure than control samples.

Endovascular Treatment for Lower Extremity Deep Vein Thrombosis: An Overview

Kim Kyung Ah,Choi Sun Young,Kim Ran 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.6

Lower extremity deep vein thrombosis (DVT) is a serious medical condition that can result in local pain and gait disturbance. DVT progression can also lead to death or major disability as a result of pulmonary embolism, postthrombotic syndrome, or limb amputation. However, early thrombus removal can rapidly relieve symptoms and prevent disease progression. Various endovascular procedures have been developed in the recent years to treat DVT, and endovascular treatment has been established as one of the major therapeutic methods to treat lower extremity DVT. However, the treatment of lower extremity DVT varies according to the disease duration, location of affected vessels, and the presence of symptoms. This article reviews and discusses effective endovascular treatment methods for lower extremity DVT.

MTHFR 3'-untranslated region polymorphisms contribute to recurrent pregnancy loss risk and alterations in peripheral natural killer cell proportions

Kim, Eun Sun,Kim, Jung Oh,An, Hui Jeong,Sakong, Jung Hyun,Lee, Hyun Ah,Kim, Ji Hyang,Ahn, Eun Hee,Kim, Young Ran,Lee, Woo Sik,Kim, Nam Keun The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.3

Objective: To identify the associations between polymorphisms of the 3'-untranslated region (UTR) of methylenetetrahydrofolate reductase (MTHFR) gene, which codes for an important regulatory enzyme primarily involved in folate metabolism, and idiopathic recurrent pregnancy loss (RPL) in Korean women. Methods: The study population comprised 369 RPL patients and 228 controls. MTHFR 2572C > A, 4869C > G, 5488C > T, and 6685T > C 3'-UTR polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis or by TaqMan allelic discrimination assays. Natural killer cell proportions were determined by flow cytometry. Results: The MTHFR 2572-5488-6685 (A-C-T) haplotype had an adjusted odds ratio of 0.420 (95% confidence interval, 0.178-0.994; p= 0.048) for RPL. Analysis of variance revealed that MTHFR 4869C > G was associated with altered $CD56^+$ natural killer cell percentages (CC, $17.91%{\pm}8.04%$; CG, $12.67%{\pm}4.64%$; p= 0.024) and folate levels (CC, $12.01{\pm}7.18mg/mL$; CG, $22.15{\pm}26.25mg/mL$; p= 0.006). Conclusion: Variants in the 3'-UTR of MTHFR are potential biomarkers for RPL. However, these results should be validated in additional studies of ethnically diverse groups of patients.

S-14 Sphincter of Oddi function after EPLBD by endoscopic manometry.

( Ah Ran Kim ),( Young Koog Cheon ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Background and Aims: Endoscopic papillary balloon dilation ( 8 mm in diameter) preserves sphincter of Oddi (SO) function. However, it is still unknown whether papillary function is preserved after endoscopic papillary large balloon dilation (EPLBD,312 mm in diameter). We investigated SO function after EPLBD with or without endoscopic sphincterotomy (EST) by endoscopic manometry, up to 1 year post-procedure.?Methods: This was a prospective randomized study involving patients with bile duct stones 12 mm. Eighty-six patients who met the inclusion criteria were assigned randomly to either the EPLBD alone or EST with EPLBD groups, and endoscopic manometric studies were performed. The primary outcome was comparison of the manometric data between the two groups and within each group both 1 week and 1 year post-procedure.?Results: One week after EPLBD alone and EST with EPLBD, the basal pressure of SO dropped from 30.4 to 6.4 mmHg (p<.001) and 29.5 to 2.9 mmHg (p<.001), respectively. SO function was not recovered at 1 year, as the manometric measures were similar to those taken at the 1-week time point in both groups. Similar outcomes were obtained in patients with EPLBD alone compared with those with EST and EPLBD, including initial stone clearance rate (95.2% vs. 97.7%, p=.612), frequency of mechanical lithotripsy (21.4% vs. 13.6%), and overall complications (11.9% vs. 13.6%, p=1.0) including post-procedure pancreatitis rate (7.1% vs. 11.4%, p=.714). During an overall mean median follow-up of 18.9 17.8 months, the bile duct stone recurrence rate was 16.7% in EPLBD alone patients and 15.9% in EST with EPLBD patients (p=.924).?Conclusions: Both EPLBD alone and EST+EPLBD resulted in persistent and comparable loss of SO function after one year. EPLBD alone has similar efficacy and safety to those of EST with EPLBD with respect to removal of large stones.

Complete mitochondrial genome of Palaemon gravieri (Yu, 1930) (Crustacea: Decapoda: Palaemonidae)

Kim, Sung-Tae,Kim, Gyungryul,Yoon, Tae-Ho,Kim, Ah-Ran,Kim, Hak Jun,Kim, Hyun-Woo Informa UK (Informa Healthcare) 2017 Mitochondrial DNA. Part A Vol.28 No.1

Differentiation of Dopaminergic Neurons from Mesenchymal-Like Stem Cells Derived from Human Umbilical Cord Vein

Kim Ju-Ran,Lee Jin-Ha,Jalin Anjela Melinda,Lee Chae-Yeon,Kang Ah-Reum,Do Byung-Rok,Kim Hea-Kwon,Kam Kyung-Yoon,Kang Sung-Goo 한국발생생물학회 2009 발생과 생식 Vol.13 No.3

One of the most extensively studied populations of multipotent adult stem cells are mesenchymal stem cells (MSCs). MSCs derived from the human umbilical cord vein (HUC-MSCs) are morphologically and immunophenotypically similar to MSCs isolated from bone marrow. HUC-MSCs are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. Since neural tissue has limited intrinsic capacity of repair after injury, the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesenchymal-like stem cells from the human umbilical cord vein, and studied transdifferentiation-promoting conditions in neural cells. Dopaminergic neuronal differentiation of HUC-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulfoxide (DMSO) and butylated hydroxyanisole (BHA) in N2 medium and N2 supplement. The immunoreactive cells for -tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. HUC-MSCs treated with bFGF, SHH and FGF8 were differentiated into dopaminergic neurons that were immunopositive for tyrosine hydroxylase (TH) antibody. HUC-MSCs treated with DMSO and BHA rapidly showed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including NeuroD1, -tubulin III, GFAP and nestin was markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after neural differentiation, we confirmed the differentiation of dopaminergic neurons by TH/-tubulin III positive cells. In conclusion, HUC-MSCs can be differentiated into dopaminergic neurons and these findings suggest that HUC-MSCs are alternative cell source of therapeutic treatment for neurodegenerative diseases.

Synthesis of Methyl-substituted Bicyclic Carbanucleoside Analogs as Potential Antiherpetic Agents

Kim, Kyung-Ran,Park, Ah-Young,Lee, Hyung-Rock,Kang, Jin-Ah,Kim, Won-Hee,Chun, Pu-Soon,Bae, Jang-Ho,Jeong, Lak-Shin,Moon, Hyung-Ryong Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.10

Novel bicyclo[3.1.0]hexanyl purine nucleoside analogues were synthesized as potential antiherpetic agents via a bicyclo[3.1.0]hexanol (${\pm}$)-8, which was prepared using a highly efficient carbenoid cycloaddition reaction. A highly diastereoselective reduction of ketone and a Mitsunobu reaction for the condensation of glycosyl donor (${\pm}$)-12 with 6-chloropurine were employed.