Genetic Variation of Esterase Isozrymes of Larval Haemolymph in Relation to Geographical Origin in the Silkworm, (Bombyx mori)

Abid Ali Khan,Moon Jae Yu,Lee,Sang Mong 서울大學校 農科大學 1988 서울대농학연구지 Vol.13 No.1

공시잠품종으로서 96품종중 일본종계 30, 중국종계 33, 유럽종계 24, 열대종계 9품종을 사용하여 계통별 잠품종간의 esterase isozyme의 전기영동적 이동도에 따라 유전적 변이 및 zymogram pattern으로 원산지별 잠품종의 유전적 상호관계를 알기 위하여 본 실험을 수행하였으며, 그 결과를 요약하면 다음과 같다. 1. 96품종의 누에체액의 esterase isozyme를 전기영동적 이동도에 따라 Est 1, Est 2, Est 3, Est 4, Est 5, Est 6, Est 7으로서 모두 7개의 양극으로 이동되는 isozyme을 검출하였다. 2. 원산지 게통별 esterase isozyme의 활성대수는 평균 2∼5개였다. 3. 7종의 esterase band는 그 표현형에 따라 10개의 symogram Pattern(T1-T10)으로 분류되었다. 4. 검출된 esterase isozyme의 대부분은 그 이동도에 따라 느린 부분, 중간부분, 빠른부분의 3부분으로 나누어졌다. 5. 모든 품종에서 공통적으로 검출된 esterase isozyme은 Est 2, Est 6, Est 7이었다. 6. 일본종에만 특이적으로 검출된 esterase는 Est 3이었다. 7. 각각의 esterase band는 이동도가 서로 달랐으나, 일본종 및 중국종에 있어서는 일본종의 Est 3를 제외하고는 서로 비슷한 변이를 보였다. 8. Est 1,Est 2, Est 3, Est 4, Est 5, Est 6,Est7의 계통별 출현 빈도는 일본종계 : 0%, 100%, 33%, 56.6%, 96.6%, 10%, 96.6% 중국종계 : 6.1%, 100%, 0%, 27.3%, 97%, 33.3%, 100% 유럽종계 : 4.2%, 95.8%, 0%, 29.2%, 95.8%, 8.3%, 100% 열대종계 : 0%, 100%, %, 0%, 0%, 100%, 100%였다. 9. 열대종계의 모든 품종은 다같이 Est 2, Est 6, Est 7 등 3개의 band를 공통적으로 가지고 있었다. 10. 모든 계통의 품종당 esterase isozyme의 band수는 2, 3, 4, 5개로서 그 빈도는 일본종계가 0%, 3.3%, 20%, 66.6%, 0%, 0%, 중국종계가 3%, 30.3%, 63.6%, 3%이었고 유럽종계는 3개 및 4개의 band가 각각 50%, 열대종계는 모든 품종 다같이 3개의 band가 100%이었다. 11. 日本種系의 表現型 T9와 中國種系의 T6은 가장 적은 수의 band를 가지고 있었고, 日本種에 만 特異的으로 存在하는 表現型은 T3으로서 3개의 band를 가지고 있었다. 12. 일본종계 및 중국종계가 다른 계통들 보다 esterase isozyme 변이가 심하여 유전적 다양성을 보였으며 중국종계가 일본종계보다 넓은 변이를 보임으로 유전변이의 중심은 중국종이라 생각된다.

Lithium ameliorates lipopolysaccharide-induced neurotoxicity in the cortex and hippocampus of the adult rat brain

Khan, M.S.,Ali, T.,Abid, M.N.,Jo, M.H.,Khan, A.,Kim, M.W.,Yoon, G.H.,Cheon, E.W.,Rehman, S.U.,Kim, M.O. Pergamon Press 2017 Neurochemistry International Vol.108 No.-

Lithium an effective mood stabilizer, primary used in the treatment of bipolar disorders, has been reported as a protective agent in various neurological disorders. In this study, we examined the neuroprotective role of lithium chloride (LiCl) against lipopolysaccharide (LPS) in the cortex and hippocampus of the adult rat brain. We determined that LiCl -attenuated LPS-induced activated toll-like receptor 4 (TLR4) signalling and significantly reduced the nuclear factor-<SUB>k</SUB>B (NF-<SUB>K</SUB>B) translation factor and various other inflammatory mediators such as interleukin-1 beta (IL-1β) and tumour necrosis factor alpha (TNF-α). We also analyzed that LiCl significantly abrogated activated gliosis via attenuation of specific markers for activated microglia, ionized calcium-binding adaptor molecule (Iba-1) and astrocytes, glial fibrillary acidic protein (GFAP) in both the cortex and hippocampus of the adult rat brain. Furthermore, we also observed that LiCl treatment significantly ameliorated the increase expression level of apoptotic neurodegeneration protein markers Bax/Bcl2, activated caspase-3 and poly (ADP-ribose) polymerase-1 (PARP-1) in the cortex and hippocampus regions of the LPS-treated adult rat brain. In addition, the morphological results of the fluoro-jade B (FJB) and Nissl staining showed that LiCl attenuated the neuronal degeneration in the cortex and hippocampus regions of the LPS-treated adult rat brain. Taken together, our Western blot and morphological results indicated that LiCl significantly prevents the LPS-induced neurotoxicity via attenuation of neuroinflammation and apoptotic neurodegeneration in the cortex and hippocampus of the adult rat brain.

Supergene Alteration of Amphibole in Kaya San Kaolin Deposit : Mineralogical and Morphological Studies

( Abid Murtaza Khan,Soo Jin Kim,Dong Jin Lee ) 한국광물학회 1989 광물과 암석 (J.Miner.Soc.Korea) Vol.2 No.1

Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

( Shehzad Abid Khan ),( Hyung Min Kim ),( Ju Hye Baek ),( Hye Su Jung ),( Che Ok Jeon ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242^T and B156^T, were isolated from soil in South Korea. Cells of strain H242^T were oxidase-positive and non-motile short rods, while those of strain B156^T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C_16:0, cyclo-C_17:0, and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242^T and B156^T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242^T and B156^T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242^T and B156^T was 24.6%. Strains H242^T and B156^T were most closely related to Ramlibacter ginsenosidimutans BXN5-27^T and Ramlibacter monticola G-3-2^T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242^T and R. ginsenosidimutans and between strain B156^T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242^T and B156^T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242^T (=KACC 21667 ^T =JCM 33922^T) and B156T (=KACC 21665 ^T =JCM 33920^T), respectively.

A Simple and Cost-effective Design for Simultaneous Transmission of Point-to-point and Broadcast Services in WDM-PON

Ahmed Muddassir Khan,Zhang Jie,Yousaf Khan,Muhammad Idrees,Yongli Zhao,Shahab Niazi,Aftab Husein,Abid Munir,Ishtiaq Ahmed,Junyan Liu 보안공학연구지원센터 2013 International Journal of Future Generation Communi Vol.6 No.3

Adiponectin homolog novel osmotin protects obesity/diabetes-induced NAFLD by upregulating AdipoRs/PPARα signaling in <i>ob/ob</i> and <i>db/db</i> transgenic mouse models

Ahmad, Ashfaq,Ali, Tahir,Kim, Min Woo,Khan, Amjad,Jo, Myeung Hoon,Rehman, Shafiq Ur,Khan, Muhammad Sohail,Abid, Noman Bin,Khan, Mehtab,Ullah, Rahat,Jo, Min Gi,Kim, Myeong Ok Elsevier 2019 clinical and experimental Vol.90 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>In metabolic disorders, adiponectin and adiponectin receptors (AdipoR1/R2) signaling has a key role in improving nonalcoholic fatty liver disease (NAFLD) in obesity-associated diabetes.</P> <P><B>Objective</B></P> <P>To the best of our knowledge, here, we reported for the first time the underlying mechanistic therapeutic efficacy of the novel osmotin, a homolog of mammalian adiponectin, against NAFLD in leptin-deficient <I>ob/ob</I> and <I>db/db</I> mice.</P> <P><B>Methods</B></P> <P>The <I>ob/ob</I> and <I>db/db</I> mice were treated with osmotin at a dose of 5 μg/g three times a week for two weeks. To co-relate the <I>in vivo</I> results we used the human liver carcinoma HepG2 cells, subjected to knockdown with small siRNAs of AdipoR1/R2 and PPARα genes and treated with osmotin and palmitic acid (P.A.). MTT assay, Western blotting, immunohistofluorescence assays, and plasma biochemical analyses were applied.</P> <P><B>Results</B></P> <P>Osmotin stimulated AdipoR1/R2 and its downstream APPL1/PPAR-α/AMPK/SIRT1 pathways in <I>ob/ob</I> and <I>db/db</I> mice, and HepG2 cells exposed to P.A. Mechanistically, we confirmed that knockdown of AdipoR1/R2 and PPARα by their respective siRNAs abolished the osmotin activity in HepG2 cells exposed to P.A. Overall, the <I>in vivo</I> and <I>in vitro</I> results suggested that osmotin protected against NAFLD through activation of AdipoR1/R2 and its downstream APPL1/PPAR-α/AMPK/SIRT1 pathways as shown by the reduced body weight, blood glucose level and glycated hemoglobin, improved glucose tolerance, attenuated insulin resistance and hepatic glucogenesis, regulated serum lipid parameters, and increased fatty acid oxidation and mitochondrial functions.</P> <P><B>Conclusion</B></P> <P>Our findings strongly suggest that novel osmotin might be a potential novel therapeutic tool against obesity/diabetes-induced NAFLD and other metabolic disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Osmotin <I>via</I> AdipoRs dependently reduced palmitic acid-induced toxicity <I>in vitro</I>. </LI> <LI> Osmotin regulated AdipoRs/APPL1/PPAR-α/AMPK/SIRT1 pathways in <I>ob/ob</I> and <I>db/db</I> mice. </LI> <LI> Osmotin regulated AdipoRs/APPL1/PPAR-α/AMPK/SIRT1 pathways in HepG2 cells. </LI> <LI> Osmotin regulated the impaired insulin signaling both <I>in vivo</I> and <I>in vitro</I> studies. </LI> <LI> Osmotin treatment regulated plasma chemistry associated with metabolic disorders. </LI> </UL> </P>

The effect of bolt tightening methods and sequence on the performance of gasketed bolted flange joint assembly

Abid, Muhammad,Khan, Yasir Mehmood Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.46 No.6

This paper presents results of the effect of different bolt tightening sequences and methods on the performance of gasketed bolted flange joint using nonlinear finite element analysis. Bolt preload scatter due to elastic interactions, flange stress variation and bolt bending due to flange rotation and gasket contact stress variation is difficult to eliminate in torque control method i.e. tightening one bolt at a time. Although stretch control method (tightening more than one bolt at time) eradicates the bolt preload scatter, flange stress variation is relatively high. Flange joint's performance is compared to establish relative merits and demerits of both the methods and different bolt tightening sequences.

Arsenic(V) biosorption by charred orange peel in aqueous environments

Abid, Muhammad,Niazi, Nabeel Khan,Bibi, Irshad,Farooqi, Abida,Ok, Yong Sik,Kunhikrishnan, Anitha,Ali, Fawad,Ali, Shafaqat,Igalavithana, Avanthi Deshani,Arshad, Muhammad Taylor Francis 2016 International journal of phytoremediation Vol.18 No.5

Multi-aging Effects on Vegetable Based Oils for Transformer Insulation in HV Systems

Khan Irfanullah,Abid Muhammad Ahtasham,Ullah Kaleem,Ullah Zahid,Haider Aun,Ahmad Farhan Ammar,Zia Zain,Ali Sahibzada Muhammad 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.5

Liquid insulation media is used for insulation and cooling purpose inside the transformer. Currently, transformers are using petroleum based mineral oil, which poses a serious hazardous environmental impact, since the mineral oil is non-renewable and non-biodegradable. Although, the increased cost and depleting nature of mineral oil cause an emergent need to use suitable alternatives to mineral oils that are biodegradable and environmentally friendly. In response to this resource issue, various vegetable oils, namely Sunfl ower oil, Soya bean oil, and a blend of Sunfl ower and Olive (BSO) oil are suitable alternatives for transformer insulation in high voltage systems. The afore-mentioned vegetable oils are subjected to multi-aging and comparative analysis is performed with mineral oil. Further, the dielectric and thermal properties of vegetable oils are tested before and after aging. Fourier Transform Infrared spectroscopy, water content, breakdown voltage, viscosity, fl ash point, tan delta, and pour point tests are performed on vegetable oils before and after aging. Finally, a comparative analysis of vegetable oils with mineral oil is provided to prove the effi cacy of the proposed vegetable oils. The BSO oil resulted in higher breakdown strength and good thermal behavior when subjected to the abovementioned various diagnostic measurement tests in comparison to other oils

Train Scheduling and Rescheduling In Pakistan

Abid, Malik Muneeb,Khan, Muhammad Babar The Korean Society for Railway 2013 International Journal of Railway Vol.6 No.1

This paper provides an overview of Pakistan Railways scheduling and rescheduling. First of all, Pakistan Railways is introduced with its brief history and importance in this country. Assets of this network with passengers and freight using this are given. Current hope less situation is leading to privatization of this system as well as promoting short distance traveler to use road and Government efforts to retain is also presented. Train scheduling in Pakistan is being done manually, based on manual time distance graph preparation and resolution of conflicts based on manager's experience and ability. In Real-time management of this traffic Lahore head office is connected with six control stations in the Pakistan, decision for resolution of any disturbance is coordinated among them. It is recommended that computer aided tools must be developed for this system to help traffic managers and it is needed to invest on the segments to increase their speed limits which might attract passengers to use this mode of transportation with high priority.