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( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3
Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.
Endophytic Fungal strains Specifically Modified the Biochemical Status of Grape Cells
Li-Hua Huang,Ming-Zhi Yang,Xiu-Jin Ao,An-Yun Ren,Ming-Quan Yuan,Han-Bo Zhang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.4
Previously, specific interactions in morphologywere observed between grape cells and endophytic fungalstrains during a dual culture experiment. However, thebiochemical impacts of these fungal endophytes on grapecells is also expected due to their potential application in grapequality management. After assessed multiple physiochemicaltraits to those grape cells which have co-cultured with differentendophytic fungal strains in this study, and found the presenceof fungal endophytes obviously triggered ROS stress responsesin grape cells, and the biochemical status in grape cells weredifferentially modified by different fungal strains. In thosetested endophytic fungal strains, RH37 (Epicoccum sp.),RH6 (Alternaria sp.), RH32 (Alternaria sp.) and RH34(Trichothecium sp.) conferred greater metabolic impacts ongrape cells. And soluble protein (SPr), total flavonoids (TF),total phenols (TPh) and malondialdehyde (MDA) on the otherhand, were sensitive biochemical parameters which can beinfluenced in greater ranges than other detected parameters. Most interestedly, fungal endophytes shaped metabolitespatterns in grape cells during the dual culture appeared fungalgenus/species/strain-specificity. The work confirmed thesignificance of fungal endophytes in grape metabolic regulationand elucidated the possibility to purposely manage grapequality using tool of fungal endophytes.
Wang, X L,Dou, S X,Ren, Zhi-An,Yi, Wei,Li, Zheng-Cai,Zhao, Zhong-Xian,Lee, Sung-IK IOP Pub 2009 Journal of physics, an Institute of Physics journa Vol.21 No.20
<P>We measured the initial <I>M</I>–<I>H</I> curves for a sample of the newly discovered superconductor NdFeAsO<SUB>0.82</SUB>Fe<SUB>0.18</SUB>, which had a critical temperature, <I>T</I><SUB>c</SUB>, of 51 K and was fabricated at the high pressure of 6 GPa. The lower critical field, <I>H</I><SUB>c1</SUB>, was extracted from the deviation point of the Meissner linearity in the <I>M</I>–<I>H</I> curves, which show linear temperature dependence in the low temperature region down to 5 K. The <I>H</I><SUB>c1</SUB>(<I>T</I>) indicates no s-wave superconductivity, but rather an unconventional superconductivity with a nodal gap structure. Furthermore, the linearity of <I>H</I><SUB>c1</SUB> at low temperature does not hold at high temperature, but shows other characteristics, indicating that this superconductor might have multi-gap features. Based on the low temperature nodal gap structure, we estimate that the maximum gap magnitude Δ<SUB>0</SUB> = (1.6 ± 0.2) <I>k</I><SUB>B</SUB><I>T</I><SUB>c</SUB>.</P>
( Yan Ii Bai ),( De Juan Zhi ),( Chan He Li ),( Dong Iing Liu ),( Ju An Zhang ),( Jing Ti An ),( Xin Wang ),( Hui Ren ),( Hong Yu Li ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9
Xanthomonas oryzae pv. oryzae (Xoo) strains are plant pathogenic bacteria that can cause serious blight of rice, and their virulence towards plant host is complex, making it difficult to be elucidated. Caenorhabditis elegans has been used as a powerful model organism to simplify the host and pathogen system. However, whether the C. elegans is feasible for studying plant pathogens such as Xoo has not been explored. In the present work, we report that Xoo strains PXO99 and JXOIII reduce the lifespan of worms not through acute toxicity, but in an infectious manner; pathogens proliferate and persist in the intestinal lumen to cause marked anterior intestine distension. In addition, Xoo triggers (i) the p38 MAPK signal pathway to upregulate its downstream C17H12.8 expression, and (ii) the DAF-2/DAF-16 pathway to upregulate its downstream gene expressions of mtl-1 and sod-3 under the condition of daf-2 mutation. Our findings suggest that C. elegans can be used as a model to evaluate the virulence of Xoo phytopathogens to host.
Current-voltage Characteristics of NdFeAsO0.85F0.15 and NdFeAsO0.85 Superconductors
Yong Liu,YiSheng Chai,Hyeong-Jin Kim,G.R. Stewart,김기훈,Zhi-An Ren,Zhong-Xian Zhao 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.2
The vortex phase diagrams of NdFeAsO0.85F0.15 and NdFeAsO0.85 superconductors are determined from an analysis of resistivity and current-voltage (I-V ) measurements in magnetic fields up to 9 T. A vortex glass to liquid transition can be identified only in the oxygen-deficient NdFeAsO0.85, for which the I-V curves can be well scaled onto liquid and glass branches consistent with the vortex glass theory. With increasing magnetic field, the activation energy, U0, deduced from the Arrhenius plots of the resistivity based on the thermally-activated flux-flow model decays more quickly for NdFeAsO0.85F0.15 than for NdFeAsO0.85. Moreover, the irreversibility field, Hirr, of NdFeAsO0.85 increases more rapidly than that of NdFeAsO0.85F0.15 with decreasing temperature. These observations imply strong vortex pinning effects in the oxygen-deficient NdFeAsO0.85, presumably caused by enhanced defects and disorders. We infer that the observation of a vortex glass to liquid transition in NdFeAsO0.85 may be also related to the enhanced defects and disorder in the specimen.