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Xiangquan Li,Zhengguang Xu,Yanrong Lu,Jiarui Cui,Lixin Zhang 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.10
This paper addresses a pattern-moving-based modified model free adaptive control (PMFAC) scheme and illustrates the convergence of its tracking error for a class of nonlinear systems with unknown model and multi-threshold quantized observations. The basic idea is to consider the system’s quantization error as an external disturbance and an improved performance index function of control law is proposed from the perspective of twoplayer zero-sum game (TP-ZSG) based on the existed MFAC algorithms. The PMFAC scheme is established which mainly includes an improved tracking control law and a pseudo-partial derivative (PPD) estimation algorithm. Under certain conditions, the bounded convergence of system tracking error and the stability of PMFAC system with quantization errors can be guaranteed. The theoretical results are demonstrated by two numerical examples.
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Identification and confirmation of 14-3-3 ζ as a novel target of ginsenosides in brain tissues
Feiyan Chen,Lin Chen,Weifeng Liang,Zhengguang Zhang,Jiao Li,Wan Zheng,Zhu Zhu,Jiapeng Zhu,Yunan Zhao 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.4
Background: Ginseng can help regulate brain excitability, promote learning and memory, and resist cerebral ischemia in the central nervous system. Ginsenosides are the major effective compounds of Ginseng, but their protein targets in the brain have not been determined. Methods: We screened proteins that interact with the main components of ginseng (ginsenosides) by affinity chromatography and identified the 14-3-3 ζ protein as a potential target of ginsenosides in brain tissues. Results: Biolayer interferometry (BLI) analysis showed that 20(S)-protopanaxadiol (PPD), a ginseng saponin metabolite, exhibited the highest direct interaction to the 14-3-3 ζ protein. Subsequently, BLI kinetics analysis and isothermal titration calorimetry (ITC) assay showed that PPD specifically bound to the 14-3-3 ζ protein. The cocrystal structure of the 14-3-3 ζ protein-PPD complex showed that the main interactions occurred between the residues R56, R127, and Y128 of the 14-3-3 ζ protein and a portion of PPD. Moreover, mutating any of the above residues resulted in a significant decrease of affinity between PPD and the 14-3-3 ζ protein. Conclusion: Our results indicate the 14-3-3 ζ protein is the target of PPD, a ginsenoside metabolite. Crystallographic and mutagenesis studies suggest a direct interaction between PPD and the 14-3-3 ζ protein. This finding can help in the development of small-molecular compounds that bind to the 14-3-3 ζ protein on the basis of the structure of dammarane-type triterpenoid.
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You Li,Mulan Tang,Shuxin Xu,Shuchao Zhang,Yuxin Zhai,Jiarong Yin,Zhengguang Zou 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-
The NASICON-type solid electrolyte structure of Li1.3Al0.3Ti1.7(PO4)3 (LATP) exhibits good electrochemicalperformance and thermal stability, and has been promising as a solid electrolyte. Here, a stable goodstabilityLATP precursor spinning solution was prepared using the sol–gel method for the first time. A linearLATP solid electrolyte with an oriented ordered structure was obtained using improved electrostaticspinning equipment. The sintering process regime of the LATP-ordered construction was determined. Theionic conductivity of the prepared LATP-PEO/LiClO4-PEG composite solid-state electrolyte with anordered structure was as high as 2.05 10-4 Scm1 at room temperature (25 C), one order of magnitudehigher than the ionic conductivity of the LATP composite solid-state electrolyte reported so far. Organicsolid-state electrolytes to protect LATP-ordered structured solid-state electrolytes yield excellent electrochemicalstability in lithium-metal batteries.
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Wegner Alex,Casanova Florencia,Loehrer Marco,Jordine Angelina,Bohnert Stefan,Liu Xinyu,Zhang Zhengguang,Schaffrath Ulrich 한국미생물학회 2022 The journal of microbiology Vol.60 No.1
Phytopathogenic fungi are known to secrete specific proteins which act as virulence factors and promote host colonization. Some of them are enzymes with plant cell wall degradation capability, like pectate lyases (Pls). In this work, we examined the involvement of Pls in the infection process of Magnaporthe oryzae, the causal agent of rice blast disease. From three Plgenes annotated in the M. oryzae genome, only transcripts of MoPL1 considerably accumulated during the infection process with a peak at 72 h post inoculation. Both, gene deletion and a constitutive expression of MoPL1 in M. oryzae led to a significant reduction in virulence. By contrast, mutants that constitutively expressed an enzymatic inactive version of MoPl1 did not differ in virulence compared to the wild type isolate. This indicates that the enzymatic activity of MoPl1 is responsible for diminished virulence, which is presumably due to degradation products recognized as danger associated molecular patterns (DAMPs), which strengthen the plant immune response. Microscopic analysis of infection sites pointed to an increased plant defense response. Additionally, MoPl1 tagged with mRFP, and not the enzymatic inactive version, focally accumulated in attacked plant cells beneath appressoria and at sites where fungal hyphae transverse from one to another cell. These findings shed new light on the role of pectate lyases during tissue colonization in the necrotrophic stage of M. oryzae's life cycle.
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Analysis of multi-coalbed CBM development methods in western Guizhou, China
Zhaobiao Yang,Yong Qin,Tongsheng Yi,Jun Tang,Zhengguang Zhang,Congcong Wu 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.2
After more than 20 years of development, the exploration and production of multi-bed coalbed methane in western Guizhou is now in a key growth phase. Based on geological characteristics of CBM and requirements of main development engineering in this area, we have looked into suitable fracturing and drainage modes for this area. This study shows that the Liupanshui coalfield has a high stress field, so staged fracturing with large discharge and high volume of fracturing fluid and sand and increase of the used quantity of high strength ceramic beads is effective for this kind of multi-bed coal reservoir. In contrast, Zhina coalfield has a relatively low stress field, and the Qianbei coalfield has transitional stress conditions. Separate layer fracturing by sleeve diverter in the Zhijin block ensures effective fracturing of multi-production layers. This method enhances fracturing efficiency and decreases fracturing cost and is therefore recommended for the whole area. Coiled tubing fracturing widely used in other countries is recommended for the Zhina and Qianbei coalfields. Commingled drainage and production is considered the best choice for the development of multi-bed CBM reservoirs. The basic geologic conditions for commingled drainage production of multiple layers are that the main productive layers can be ensured to produce gas preferentially and smoothly and the critical desorption pressure liquid level of other productive layers are above the roof depth of the main productive layers. On this basis, all the layers can be produced in an orderly manner by regulating the casing pressure and liquid level depth in different draining phases.
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