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RISS 인기검색어
- 검색키워드
- 저자 : Zhaoqi Wu (검색결과 3 건)
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Simulation of Tensile Bolts in Finite Element Modeling of Semi-rigid Beam-to-column Connections
Zhaoqi Wu,Sumei Zhang,Shao-Fei Jiang 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.3
This paper presents an innovative bolt model suitable for the three dimensional finite element analysis (FEA) of the semirigid beam-to-column bolted connections. The model is particularly useful for the moment-rotation relationship of beam-tocolumn connections, especially in cases where the connectors such as endplates, angles, T-stubs, are not particularly thin. In this paper, the bolt tensile behavior is firstly discussed by using a refined finite element model, in which the complex geometries of both external and internal threads were modeled. Then, the bolt behavior predicted by the commonly used models was compared with that of the refined FEA to appraise the accuracy of these models. The comparison shows most of the models commonly used can not predict accurately the axial stiffness, carrying capacity and ductility of bolt simultaneously. Afterwards,an innovative bolt model was proposed and the model accorded with the refined FEA for single bolts. Finally, the proposed model was applied to analyze the moment-rotation behavior of several experimented and well documented connections with different configurations. The results indicate that the proposed model is feasible and efficient.
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Li Jie,Xu Yanan,Zhang Jiayu,Zhang Zhaoqi,Guo Han,Wei Dong,Wu Changhong,Hai Tang,Sun Hai-Xi,Zhao Yong 한국유전학회 2024 Genes & Genomics Vol.46 No.3
Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells. Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells.
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