Expression of hPOT1 in HeLa Cells and the Probability of Gene Variation of hpot1 Exon14 in Endometrial Cancer are Much Higher than in Other Cancers

Liu, Fei,Pu, Xiao-Yun,Huang, Shao-Guang,Xiang, Gui-Ming,Jiang, Dong-Neng,Hou, Gou,Huang, Di-Nan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

To investigate the expression of hPOT1 in the HeLa cell line and screen point mutations of hpot1 in different tumor tissues a two step osmotic method was used to extract nuclear proteins. EMSA was performed to determine the expression of hPOT1 in the HeLa cell line. PCR was also employed to amplify the exon14 sequence of the hpot1 gene in various of cancer tissues. A SV gel and PCR clean-up system was performed to enrich PCR products. DNAStar was used to analyse the exon14 sequence of the hpot1 gene. hPOT1 was expressed in the HeLa cell line and the signal was gradually enhanced as the amount of extracted nuclear proteins increased. The DNA fragment of exon14 of hpot1 was successfully amplified in the HeLa cell line and all cancer tissues, point mutations being observed in 2 out of 3 cases of endometrial cancer (66.7%) despite the hpot1 sequence being highly conserved. However, the sequence of hpot1 exon14 do not demonstrate point mutations in most cancer tissues. Since hPOT1 was expressed in HeLa cell and the probability of gene point variants was obviously higher in endometrial cancer than other cancers, it may be involved in the pathogenesis of gynecological cancers, especially in cervix and endometrium.

Experimental study on ultimate torsional strength of PC composite box-girder with corrugated steel webs under pure torsion

Ding, Yong,Jiang, Kebin,Shao, Fei,Deng, Anzhong Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.46 No.4

To have a better understanding of the torsional mechanism and influencing factors of PC composite box-girder with corrugated steel webs, ultimate torsional strength of four specimens under pure torsion were analyzed with Model Test Method. Monotonic pure torsion acts on specimens by eccentric concentrated loading. The experimental results show that cracks form at an angle of $45^{\circ}$ to the member's longitudinal axis in the top and bottom concrete slabs. Longitudinal reinforcement located in the center of cross section contributes little to torsional capacity of the specimens. Torsional rigidity is proportional to shape parameter ${\eta}$ of corrugation and there is an increase in yielding torque and ultimate torque of specimens as the thickness of corrugated steel webs increases.

AntagomiR-27a Targets FOXO3a in Glioblastoma and Suppresses U87 Cell Growth in Vitro and in Vivo

Ge, Yun-Fei,Sun, Jun,Jin, Chun-Jie,Cao, Bo-Qiang,Jiang, Zhi-Feng,Shao, Jun-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.

Simulation of Tensile Bolts in Finite Element Modeling of Semi-rigid Beam-to-column Connections

Zhaoqi Wu,Sumei Zhang,Shao-Fei Jiang 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.3

This paper presents an innovative bolt model suitable for the three dimensional finite element analysis (FEA) of the semirigid beam-to-column bolted connections. The model is particularly useful for the moment-rotation relationship of beam-tocolumn connections, especially in cases where the connectors such as endplates, angles, T-stubs, are not particularly thin. In this paper, the bolt tensile behavior is firstly discussed by using a refined finite element model, in which the complex geometries of both external and internal threads were modeled. Then, the bolt behavior predicted by the commonly used models was compared with that of the refined FEA to appraise the accuracy of these models. The comparison shows most of the models commonly used can not predict accurately the axial stiffness, carrying capacity and ductility of bolt simultaneously. Afterwards,an innovative bolt model was proposed and the model accorded with the refined FEA for single bolts. Finally, the proposed model was applied to analyze the moment-rotation behavior of several experimented and well documented connections with different configurations. The results indicate that the proposed model is feasible and efficient.

Experimental study on ultimate torsional strength of PC composite box-girder with corrugated steel webs under pure torsion

Yong Ding,Kebin Jiang,Fei Shao,Anzhong Deng 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.46 No.4

To have a better understanding of the torsional mechanism and influencing factors of PC composite box-girder with corrugated steel webs, ultimate torsional strength of four specimens under pure torsion were analyzed with Model Test Method. Monotonic pure torsion acts on specimens by eccentric concentrated loading. The experimental results show that cracks form at an angle of 45° to the member’s longitudinal axis in the top and bottom concrete slabs. Longitudinal reinforcement located in the center of cross section contributes little to torsional capacity of the specimens. Torsional rigidity is proportional to shape parameter η of corrugation and there is an increase in yielding torque and ultimate torque of specimens as the thickness of corrugated steel webs increases.

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

Prognostic Value of MGMT Promoter Methylation and TP53 Mutation in Glioblastomas Depends on IDH1 Mutation

Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.