The Prevalence and Genotype of Human Papillomavirus from Patients with Genital Warts in Eastern Guangdong Province

Luo, Zhao-Yun,Chen, Qiang,Yang, Hui,Lin, Min,Chen, Chan-Yu,Yang, Chun,Yang, Li-Ye Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Low-risk human papillomavirus (LR-HPV) infection is the main cause of genital warts. LRHPV genotypes 6 and 11 are associated with genital warts, but there have only been a few published studies about the genotype-specific prevalence of HPV in genital warts in China. The objective of our study was to assess the prevalence of HPV genotypes for clinical cases involving both men and women and to evaluate the potential benefit of a quadrivalent (genotypes 6, 11, 16, and 18) HPV vaccine in eastern Guangdong province of China. Materials and Methods: A total of 696 eligible patients with genital warts were enrolled during the period Aug 2009 through Oct 2014. Specimens were collected from genital warts, the HPV GenoArray test was used for HPV detection and genotyping, which could detect 21 HPV genotypes, including genotypes 6, 11, 16, and 18. Results: Among the 696 cases, 675 samples were successfully genotyped. The median age of patients was 32.1 years (range, 16-67 years). The most prevalent genotypes were HPV-6 (285/675, 42.2%), HPV-11 (265/675, 39.3%), HPV-52 (52/675, 7.7%), HPV-16 (51/675, 7.56%), HPV-81 (50/675, 7.40%) and HPV-58 (37/675, 5.48%). Low-risk genotypes predominated, with a prevalence of 96.59%. The cumulative prevalence of genotypes 6 and 11 was 78.7% (531/675), the cumulative prevalence of genotypes 16 and 18 was 11.6% (78/675), and the cumulative prevalence of genotypes 6, 11, 16, and 18 was 82.5% (557/675). Conclusions: Our results provide strong evidence that, in eastern Guangdong, different from Western countries, the most prevalent low risk HPV genotypes in patients with genital warts are 6, 11 and 81. The quadrivalent HPV vaccine could prevent 82.5% of genital warts in eastern Guangdong.

Genome-Wide Association Study of Ulcerative Colitis in Koreans Suggests Extensive Overlapping of Genetic Susceptibility With Caucasians :

Yang, Suk-Kyun,Hong, Myunghee,Zhao, Wanting,Jung, Yusun,Tayebi, Naeimeh,Ye, Byong Duk,Kim, Kyung-Jo,Park, Sang Hyoung,Lee, Inchul,Shin, Hyoung Doo,Cheong, Hyun Sub,Kim, Lyoung Hyo,Kim, Hyo-Jong,Jung, Oxford University Press 2013 Inflammatory bowel diseases Vol.19 No.5

<P>Recent genome-wide association studies and meta-analyses have identified 47 susceptibility loci for ulcerative colitis (UC) in Caucasian populations. A previous genome-wide association study of UC in a Japanese population suggested marginal sharing of susceptibility loci between Caucasian and Asian populations. We performed a genome-wide association studies to identify UC susceptibility loci in a Korean population and further comparative study.</P>

Multivariate Analysis of the Prognosis of 37 Chondrosarcoma Patients

Yang, Zheng-Ming,Tao, Hui-Min,Ye, Zhao-Ming,Li, Wei-Xu,Lin, Nong,Yang, Di-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: The current study aimedto screen for possible factors which affect prognosis of chondrosarcoma. Methods: Thirty seven cases were selected and analyzed statistically. The patients received surgical treatment at our hospital between December 2005 and March 2008. All of them had complete follow-up data. The survival rates were calculated by univariate analysis using the Kaplan-Meier method and tested by Log-rank. ${\chi}^2$ or Fisher exact tests were carried out for the numeration data. The significant indexes after univariate analysis were then analyzed by multivariate analysis using COX regression model. Based on the literature, factors of gender, age, disease course, tumor location, Enneking grades, surgical approaches, distant metastasis and local recurrence were examined. Results: Univariate analysis showed that there were significant differences in Enneking grades, surgical approaches and distant metastasis related to the patients' 3-year survival rate after surgery (P<0.001). No significant difference was not found in gender, age, disease course, tumor location or local recurrence (P>0.05). Multivariate analysis showed that Enneking grade (P=0.007) and surgical approaches (P=0.010) were independent factors affecting the prognosis of chondrosarcoma, but distant metastasis was not (P=0.942). Conclusion: Enneking grades, surgical approaches and distant metastasis are risk factors for prognosis of chondrosarcoma, among which the former two are independent factors.

A common missense variant in NUDT15 confers susceptibility to thiopurine-induced leukopenia

Yang, Suk-Kyun,Hong, Myunghee,Baek, Jiwon,Choi, Hyunchul,Zhao, Wanting,Jung, Yusun,Haritunians, Talin,Ye, Byong Duk,Kim, Kyung-Jo,Park, Sang Hyoung,Park, Soo-Kyung,Yang, Dong-Hoon,Dubinsky, Marla,Lee, Nature Pub. Co 2014 Nature genetics Vol.46 No.9

Thiopurine therapy, commonly used in autoimmune conditions, can be complicated by life-threatening leukopenia. This leukopenia is associated with genetic variation in TPMT (encoding thiopurine S-methyltransferase). Despite a lower frequency of TPMT mutations in Asians, the incidence of thiopurine-induced leukopenia is higher in Asians than in individuals of European descent. Here we performed an Immunochip-based 2-stage association study in 978 Korean subjects with Crohn's disease treated with thiopurines. We identified a nonsynonymous SNP in NUDT15 (encoding p.Arg139Cys) that was strongly associated with thiopurine-induced early leukopenia (odds ratio (OR) = 35.6; P<SUB>combined</SUB> = 4.88 × 10<SUP>−94</SUP>). In Koreans, this variant demonstrated sensitivity and specificity of 89.4% and 93.2%, respectively, for thiopurine-induced early leukopenia (in comparison to 12.1% and 97.6% for TPMT variants). Although rare, this SNP was also strongly associated with thiopurine-induced leukopenia in subjects with inflammatory bowel disease of European descent (OR = 9.50; P = 4.64 × 10<SUP>−4</SUP>). Thus, NUDT15 is a pharmacogenetic determinant for thiopurine-induced leukopenia in diverse populations.

Genome-wide association study of Crohn's disease in Koreans revealed three new susceptibility loci and common attributes of genetic susceptibility across ethnic populations

Yang, Suk-Kyun,Hong, Myunghee,Zhao, Wanting,Jung, Yusun,Baek, Jiwon,Tayebi, Naeimeh,Kim, Kyung Mo,Ye, Byong Duk,Kim, Kyung-Jo,Park, Sang Hyoung,Lee, Inchul,Lee, Eun-Ju,Kim, Won Ho,Cheon, Jae Hee,Kim, BMJ Publishing Group Ltd 2014 Gut Vol.63 No.1

<P><B>Objective</B></P><P>Crohn's disease (CD) is an intractable inflammatory bowel disease (IBD) of unknown cause. Recent meta-analysis of the genome-wide association studies (GWAS) and Immunochip data identified 163 susceptibility loci to IBD in Caucasians, however there are limited studies in other populations.</P><P><B>Methods</B></P><P>We performed a GWAS and two validation studies in the Korean population comprising a total of 2311 patients with CD and 2442 controls.</P><P><B>Results</B></P><P>We confirmed four previously reported loci: <I>TNFSF15</I>, <I>IL23R</I>, the major histocompatibility complex region, and the <I>RNASET2-FGFR1OP-CCR6</I> region. We identified three new susceptibility loci at genome-wide significance: rs6856616 at 4p14 (OR=1.43, combined p=3.60×10<SUP>−14</SUP>), rs11195128 at 10q25 (OR=1.42, combined p=1.55×10<SUP>−10</SUP>) and rs11235667 at 11q13 (OR=1.46, combined p=7.15×10<SUP>−9</SUP>), implicating <I>ATG16L2</I> and/or <I>FCHSD2</I> as novel susceptibility genes for CD. Further analysis of the 11q13 locus revealed a non-synonymous single nucleotide polymorphism (SNP) (R220W/rs11235604) in the evolutionarily conserved region of <I>ATG16L2</I> with stronger association (OR=1.61, combined p=2.44×10<SUP>−12</SUP>) than rs11235667, suggesting <I>ATG16L2</I> as a novel susceptibility gene for CD and rs11235604 to be a potential causal variant of the association. Two of the three SNPs (rs6856616 (p=0.00024) and rs11195128 (p=5.32×10<SUP>−5</SUP>)) showed consistent patterns of association in the International IBD Genetics Consortium dataset. Together, the novel and replicated loci accounted for 5.31% of the total genetic variance for CD risk in Koreans.</P><P><B>Conclusions</B></P><P>Our study provides new biological insight to CD and supports the complementary value of genetic studies in different populations.</P>

The Influence of Epoxy Functionalized Acrylate Particles on the Properties of Plasticized PLA Blown Films

Ye Zhang,Yan Zhao,Hongwei Pan,Xianzhong Lang,Huili Yang,Huiliang Zhang,Huixuan Zhang,Lisong Dong 한국고분자학회 2016 폴리머 Vol.40 No.3

Polylactide (PLA) was plasticized with poly(diethylene glycol adipate) (PDEGA). The plasticized PLA was further blended with core-shell structured particles of glycidyl methacrylate-functionalized methyl methacrylate-butyl acrylate copolymer (GACR) using a twin-screw extruder, and the extruded samples were blown using the blown thin film technique. Both PDEGA and GACR significantly influenced the physical properties of the films. Compared to neat PLA, the elongation at break and tear strength of the films were significantly improved. The shear yielding induced by cavitation of GACR particles was the major tearing mechanism. GACR could act as a tear resistance modifier for PLA blown films. The spherulite size of the PLA/PDEGA/GACR films decreased with the addition of GACR. The biodegradability of the PLA/PDEGA/GACR films decreased slightly. These findings contributed new knowledge to the additive area and gave important implications for designing and manufacturing polymer packaging materials.

Microsatellite-based Genetic Diversity and Evolutionary Relationships of Six Dog Breeds

Ye, J.-H.,Ren, D.-R.,Xie, A.-F.,Wu, X.-P.,Xu, L.,Fu, P.-F.,Zhao, H.-A.,Yang, Qianyong Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8

The Tibetan Mastiff is one of the most archaic, ferocious and the largest dogs in the world. The Kunming dog is the chief working-dog breed in China. In this study, ten microsatellite loci were used to assess the genetic diversity and evolutionary relationships in six dog breeds, including Tibetan Mastiff, Kunming dog, Belgian Malinois, Labrador Retriever, English Springer Spaniel, and German Shepherd. The highest genetic diversity was exhibited by the Tibetan Mastiff, indicating useful protection and little inbreeding in the modern Tibetan Mastiff. Higher genetic diversity was observed in European breeds, supporting the hypotheses that breeders outcross their pure breed dogs occasionally to avoid deleterious effects in Europe. Evolutionary relationships showed that English Springer Spaniel and Labrador Retriever were clustered together, then with the Tibetan Mastiff, consistent with previous cluster results. German Shepherd and Kunming dog were grouped together, coinciding with the breeding history of Kunming dog. It is the first time that Tibetan Mastiff and Kunming dog have been analyzed with microsatellites.

Association of 8 Loci on Chromosome 8q24 with Prostate Carcinoma Risk in Northern Chinese Men

Zhao, Cheng-Xiao,Liu, Ming,Wang, Jian-Ye,Xu, Yong,Wei, Dong,Yang, Kuo,Yang, Ze Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Multiple genetic studies have confirmed association of 8q24 variants with susceptibility to prostate cancer (PCa). As PCa risk SNPs may also influence disease outcome, we studied here eight 8q24 risk alleles, and evaluated their role in PCa clinical covariates in northern Chinese men. Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=289) and from age-matched normal controls (n=288). Eight 8q24 SNPs were genotyped by polymerase chain reaction-high- resolution melting analysis in 577 subjects. We examined the prevalence distribution of 8q24 risk alleles and analyzed the associations between the risk allele and PCa and clinical covariates to infer their impact on aggressive PCa. Three of the eight SNPs were associated with PCa risk in northern Chinese men, including rs16901966 (OR 1.31, 95% CI 1.01-1.70, p=0.042), rs1447295 (OR 1.47, 95% CI 1.09-1.98, p=0.011) and rs10090154 (OR 1.55, 95% CI 1.14-2.12, p=0.005). Haplotype analysis based association with the risk alleles revealed significant differences between cases and controls (OR 1.43, 95%CI 0.99-2.06, p=0.049). The risk alleles rs16901966, rs1447295 and rs10090154 were associated with age at diagnosis and tumor stage as compared with controls, while rs16901966 was associated with aggressive PCa (OR 1.43, 95% CI 1.01-2.03, p=0.042). The evidence for 8q24 SNPs with PCa risk in northern Chinese men showed rs16901966, rs1447295 and rs10090154 at 8q24 (region 1, region 2) to be strongly associated with PCa and clinical covariates. The three SNPs at 8q24 could be PCa susceptible genetic markers in northern Chinese men.

Association between Higher Blood Pressure and Risk of Diabetes Mellitus in Middle-Aged and Elderly Chinese Adults

Xue Yang,Jian Chen,An Pan,Jason H.Y. Wu,Fei Zhao,Yue Xie,Yi Wang,Yi Ye,Xiong-Fei Pan,Chun-Xia Yang 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.3

Background: To examine the prospective association between higher blood pressure (BP) and risk of type 2 diabetes mellitus (T2DM) in middle-aged and elderly Chinese adults. Methods: A total of 9,642 middle-aged and elderly Chinese adults (≥45 years old; 47.30% men) without diabetes from the China Health and Retirement Longitudinal Study were included for analyses. Participants were categorized into three groups: normal BP, prehypertension, and hypertension, according to the 2010 Chinese Guidelines for the Management of Hypertension. The incidence of T2DM was determined by self-reported physician diagnosis during two follow-up surveys conducted in 2013 to 2014 and 2015 to 2016. Results: During the 4-year follow-up, 429 participants (4.45%) developed T2DM, including 3.51% of the men and 5.29% of the women. The incidence rates of T2DM were 2.57%, 3.75%, and 6.71% in the normal BP, prehypertension, and hypertension groups, respectively. After adjustment for age, sex, education level, residence, smoking status, alcohol consumption, body mass index, waist circumference, and dyslipidemia, both prehypertension (odds ratio [OR], 1.32; 95% confidence interval [CI], 0.98 to 1.77) and hypertension (OR, 2.02; 95% CI, 1.54 to 2.64) were associated with increased risk of T2DM, compared to those with a normal BP. The ORs associated with T2DM were 1.08 (95% CI, 1.03 to 1.13) for an increase of 10 mm Hg in systolic BP and 1.06 (95% CI, 1.01 to 1.10) for an increase of 5 mm Hg in diastolic BP. Conclusion: Higher BP is a risk factor for T2DM in middle-aged and elderly Chines. It may be a potential target for diabetes prevention.

3'-Hydroxyamentoflavone and Its 7-O-Methyl Ether, Two New Biflavonoids from Aristolochia contorta

Chen Ye-Gao,Yu Li-Li,Huang Rang,Liu Jia-Chuan,Lv Yu-Ping,Zhao Yang The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.11

Two new biflavonoids, 3'-hydroxyamentoflavone-7-O-methyl ether (1) and 3'-hydroxyamentoflavone (2), were isolated from the fruits of Aristolochia contorta Bge. Their structures were elucidated by HR-ESI-MS, 1D-, and 2D-NMR spectroscopy.