Machine Learning based Proximity Detection for Sparse Beacon Networks

Linlin Zhang,Li Zhu,Rong Ran 한국통신학회 2017 한국통신학회 학술대회논문집 Vol.2017 No.6

TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins

Linlin Sun,Guohong Yu,Xiaori Han,Shichao Xin,Xiaojing Qiang,Linlin Jiang,Shuhui Zhang,Xian-guo Cheng 한국식물학회 2015 Journal of Plant Biology Vol.58 No.5

Aquaporins (AQPs), a large family of channel proteins in plants, play an important role in regulating the balance of osmotic potential in cells. We isolated an AQP gene, TsMIP6, from the halophyte Thellungiella salsuginea and functionally characterized it in transgenic rice (Oryza sativa). This gene belongs to a subfamily of tonoplast intrinsic proteins and is localized at the plasma membrane. Real-time PCR showed that expression of TsMIP6 in shoots or roots of T. salsuginea was markedly induced by salinity, whereas its ectopic expression in ‘Kitaake’ lines of rice significantly increased plant tolerance to salt stress. Physiological data suggested that TsMIP6 is involved in regulating ion homeostasis and water channel activity in salt-stressed transgenic rice. Heterologous expression analysis indicated that TsMIP6 specifically interacts with a member of the glycoside hydrolase family 64 protein #617 in yeast cells. This suggests that the relationship between TsMIP6 and #617 has a crucial role in mediating osmotic balance in plant cells. Moreover, TsMIP6 might help to modulate the transport of some neutral molecules and may function through a pathway regulating solute equilibrium to maintain osmotic potential.

Production and Characterization of Monoclonal Antibodies Against Primo Vascular System of Rat

Linlin Zhang,오상욱 사단법인약침학회 2020 Journal of Acupuncture & Meridian Studies Vol.13 No.3

Background: The primo vascular system (PVS) has been difficult to detect due to its small diameter and translucent features of the threadlike network. Thus, contrast-enhancing dyes including Alcian blue, Trypan blue and Janus green B had to be used for finding and taking out PVS from rat and mouse. Objective: Generation of monoclonal antibodies (mAbs) against PVS of rat was intended to use as a detector for PVS and a biological tool for functional study of PVS. Materials and methods: Primo vessel (PV) and Primo node (PN) were isolated from organ surfaces of rat and then their proteins were isolated and injected into mouse as an immunogen. The classical traditional method was applied for production of mAbs against PVS. The various techniques, such as cell fusion, screening of hybridoma, ELISA, Western blotting (WB), immunofluorescence microscopy (IF), and limiting dilution, were used to generate mAbs against PVS. Results: Among 16 mAbs generated, 4 representative mAbs were characterized with their specificities in ELISA, WB, and IF. α-rPVS-m1-1 and α-rPVS-m4-6 had strong binding affinities to PVS in both ELISA and WB but did not show specificities in IF at all. On the contrary, α-rPVS-m3-2 and α-rPVS-m3-4 almost did not respond in WB but had strong binding affinities in ELISA and specificities in IF. Two mAbs stained predominantly at extra cellular matrix and cell membrane of PVS of rat in IF, and they were able to discriminate PVS from blood vessel (BV) and lymphatic vessel (LV). Conclusions: 4 representative mAbs against PVS of rat were characterized by ELISA, WB, and IF. α-rPVS-m3-2 and α-rPVS-m3-4, which had strong specificities in IF, can be used as a tool in discriminating PVS from other similar tissuesand in elucidate biological function of PVS.

성병을 유발하는 N. gonorrhoeae에 대한 단일클론 항체의 생산

장림림 ( Linlin Zhang ),최안나 ( Anna Choi ),오상욱 ( Sang Wook Oh ) 전북대학교 과학교육연구소 2016 科學敎育論叢 Vol.41 No.2

인간의 주요한 성인성 질환 중 가장 대표적인 질환인 임질은 사람과 사람 사이에서의 성적인 접촉을 통해 임질균(Neisseria gonorrhoea)에 감염되어 발생한다. 임질균은 남성과 여성의 비뇨생식기계에 염증을 일으키며 적절한 시기에 치료하지 않으면 불임이나 실명 등의 큰 후유증을 발생시킬 수 있다. 따라서 최근에는 적절한 시기에 정확하게 진단하여 처방하는 조기 진단의 중요성이 부각되고 있다. 본 연구의 주된 목표는 임질균에 대한 단일콜론항체를 생산하고 이를 이용하여 임질균에 대한 감염여부를 간단하고 빠르게 진단하는데 활용하고자 하였다. 임질균을 면역원으로 사용하여 생쥐에 주입하여 면역반응을 유발 시킨 후 생쥐로부터 지라를 추출하였다. 추출한 지라 세포와 골수종 암세포(myeloma SP2 cell)를 융합시킨 후 고전적인 단일클론 항체 제작법을 사용하여 임질균에 특이적으로 반응하는 항체를 생성 분비하는 하이브로도마(hybridoma) 세포를 제작하였다. 제작된 33개의 단일클론 항체는 연구재료로서 뿐만 아니라 질병에 대한 진단제로서의 유용성하게 활용될 예정이다. Gonorrhea, one of the most common adult diseases of human society, is caused by infection with gonococcus (Neisseria gonorrhoea) through sexual contact between humans and humans, such as sexual intercourse. Gonorrhea causes inflammation of the urogenital system in both males and females, and if not treated at the right time, it can cause a large aftereffect such as infertility or blindness. Therefore, in recent years, the importance of early diagnosis that accurately diagnoses and prescribes at an appropriate time is emphasized. The purpose of this study was to produce monoclonal antibody (mAb) against to N. gonorrhoea and to use for quick diagnosis of whether people were infected with N. gonorrhoea. Spleen from immunized mouse was extracted after N. gonorrhoea was injected as an immunogen. The spleen cell was fused with myeloma SP2 cell, and then hybridoma cells producing mAb against N. gonorrhoea specifically were generated by classical method for mAb. The generated 33 mAbs will be utilized for research material as well as for diagnostic reagent for diseases.

잠재적인 당뇨병 생체지표로서의 일반타액단백질(CSP1)

장림림 ( Linlin Zhang ),한미희 ( Mi Hee Han ),오상욱 ( Sang Wook Oh ) 전북대학교 과학교육연구소 2018 과학과 과학교육 논문지 Vol.43 No.1

침은 주요 침샘과 많은 타액샘에서 분비되는 체액으로 여러 가지 구성성분들을 포함하고 있다. 일반타액단백질(CSP1)은 다양한 여러 성분들 중 하나로, 기능은 아직 잘 밝혀지지 않았다. 이번 연구에서는 당뇨환자의 침의 CSP1의 농도가 정상인보다 훨씬 높은 것으로 나타났다. 단일클론 항체는 인간 타액의 웨스턴 블롯팅과 다양한 인체조직의 면역조직화학법(immunohistochemistry)에 탐침자로 사용되었다. 17명의 당뇨 환자와 9명의 정상 성인의 침 CSP1 농도는 ELISA 시스템에 의해 정량화 하였다. 다양한 인체 조직 중, 침샘은 면역조직화학법에 의해 단일클론항체-CSP1로 염색되는 유일한 조직으로 나타났다. ELISA에 의한 CSP1의 농도는 당뇨환자와 정상성인에게서 각각 4.65(3.36-6.90)μg/ml과 3.74(2.86-5.02)μg/ml인 것으로 나타났다. T-test 결과 두 그룹 사이의 CSP1 농도 값의 유의한 차이가 나타나는 것으로 보아서, CSP1이 당뇨에 대한 잠재적인 생체지표인 것으로 나타났다. Saliva is a bodily fluid secreted by three pairs of major salivary glands and by many of minor salivary gland. The human common salivary protein 1(CSP1) is one of variety of molecules in saliva and its function remains to be determined. The saliva CSP1 levels of 17 DM patients and 9 normal adults were quantified by ELISA system. As a probe, the mAb-CSP1 was used for Western blotting of human saliva and immunohistochemistry of various human tissues. The study showed that the saliva CSP1 concentration of diabetes mellitus(DM) patients was much higher than of healthy controls. The salivary gland was the only tissue stained with mAb-CSP1 by immunohistochemistry among the various human tissues. Quantification of CSP1 concentration by ELISA showed that the median values of DM patients and healthy adults were 4.65(3.36-6.90)μg/ml and 3.74(2.86-5.02)μg/ml, respectively. Student t-test results indicated that there was a statistically significant difference between the two groups(p<0.026). Therefore, the significant difference of CSP1 levels between the two groups indicated that CSP1 is a potential biomarker for detection or screenig of DM patients.

Indications of the sedimentary environments by the sedimentary characteristics and trace elements of Ordovician Majiagou Formation, Northern Shaanxi salt basin

LinLin Wang,Tao Shi,Dawei Chang,Haitao Zhang 한국자원공학회 2020 Geosystem engineering Vol.23 No.6

The abundant geochemical information on elements in the autogenous sedimentary rocks can help reconstruct the paleoenvironment of the stratigraphic sedimentation period. Based on the analysis of sedimentary characteristics of Ordovician Majiagou Formation and the trace elements in limestone, dolomite and gypsum rocks in Northern Shaanxi salt basin, the relationship between the distribution of trace elements of Majiagou Formation and the sedimentary environment is discussed. The results display that Majiagou Formation is mainly composed of the sedimentation of carbonate rocks and evaporites, and the subfacies of open platform and restricted platform are developed. The trace element analysis shows that the deposition period of the Majiagou Formation is dominated by dry hot climate, and there are alternating dry and wet seasons in climate evolution. In middle Ordovician, the sedimentary environment of Majiagou Formation was undergoing the transformation of oxygen enrichment-oxygen deficiency-anoxic environment.

A New Technology of Comprehensive Islanding Detection

Linlin Gao,Zhiyou Ren,Zhongqiang Ge,Aijun Zhang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.6

With the widely application of the distributed generation technology, micro grid islanding detection technology has been greatly developed. A new type of islanding detection technique is proposed in this paper. The new detection technology has realized the rapid, efficient, stable and reliable islanding detection, and achieved the zero non detection zone. At the same time, which put money into limit in minimum range. This paper presents the principle of islanding comprehensive detection technology. This paper designs and describes the method to realize the new islanding detection technology. The new technology makes up for a series of shortcomings,such as the non-detection zone (NDZ) is larger of the local testing technology. And it also overcomes a great many shortcomings of the remote detection technology, such as a big investment and too single.

Characterization of fermented pomegranate juice: ACE inhibitory activity under in vitro digestion, antioxidant capacity, phenolics composition, chemical properties and sensory evaluation

Lichen Zhang,Linlin Wang,Jingwei Cai,Ting Yang,Jianke Li,Guowei Shu 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.4

Consuming pomegranate juice (PJ) is beneficial for hypertensive regulation because of the phenolic compounds in PJ and their inhibitory activity on angiotensin-I-converting enzyme (ACE). To better utilize bioactive function of food, microorganism fermentation has been adopted to alter phenolic metabolism. This study confirms that even under in vitro digestion, fermented PJ (FPJ) maintains higher ACE inhibitory activity than that of PJ. The main phenolic compounds in PJ were compared either under fermentation or in vitro digestion. This study finds that fermentation promotes antioxidant capacity of PJ. The chemical properties of FPJ are evaluated and the corresponding relationship with bioactivities is analyzed. A sensory evaluation comparison is conducted between FPJ and PJ, furnishing interesting information for consumers. This study highlights the relationship between ACE inhibitory activity of PJ and phenolic composition under fermentation and in vitro digestion, providing novel insights for diet regulation of phenolic-rich FPJ in ACE inhibition therapy.

LINC00319 promotes cancer stem cell-like properties in laryngeal squamous cell carcinoma via E2F1-mediated upregulation of HMGB3

Yuan Linlin,Tian Xiufen,Zhang Yanfei,Huang Xinhui,Li Qing,Li Wencai,Li Shenglei 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Laryngeal squamous cell carcinoma (LSCC) is one of the most common subtypes of head and neck malignancies worldwide. Long intervening/intergenic noncoding RNAs (LINCRNAs) have been recently implicated in various biological processes that take place in the setting of laryngeal cancer, but the regulatory role of LINC00319 in LSCC remains largely unknown. The current study aimed to elucidate the regulatory effect of LINC00319 on the development and progression of LSCC via high-mobility group box 3 (HMGB3). Microarray-based analysis was initially conducted to identify differentially expressed long noncoding RNAs, after which the expression of LINC00319 and HMGB3 in LSCC tissues and cells was determined accordingly. CD133 + CD144 + TU177 cells were subsequently isolated and transfected with LINC00319 overexpression vector (oe-LINC00319), short hairpin RNA (sh)-LINC00319, sh-HMGB3, sh-E2F transcription factor 1 (E2F1), and oe-E2F1, as well as their corresponding controls. The proliferative, invasion, self-renewal, and tumorigenic abilities of CD133 + CD144 + TU177 cells were then evaluated. Our in vitro findings were further confirmed following subcutaneous injection of cells expressing the corresponding plasmids into nude mice. LINC00319 and HMGB3 expressions were elevated in LSCC cells and tissues. LINC00319 increased HMGB3 expression by recruiting E2F1. Furthermore, the stimulatory role of LINC00319 on the proliferation, invasion, self-renewal ability, and tumorigenicity of CD133 + CD144 + TU177 cells was achieved by upregulating HMGB3 via recruitment of E2F1. The in vitro findings were also confirmed by in vivo experiments. Taken together, these data show that downregulating LINC00319 in CD133 + CD144 + TU177 cells may serve as a potential anticancer regimen by inhibiting the proliferation and invasion of cancer stem cells in LSCC.

Dynamics of bubble formation and ascent motion on submerged orifices under different Mo number of petrol-based random copolymer solutions

Zhongyao Zhang,Xiaopeng Chen,Jiezhen Liang,Xiaojie Wei,Kaixi Deng,Jiezhong Zou,Linlin Wang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

The petrol-based random copolymer, e.g., aromatic-hydrocarbon resin, is widely used in industrial production,but the research about its gas–liquid two flow is far from being solved. Herein, the formation,motion, and deformation of a single bubble in six different concentrations of random copolymer solutionswere systemically investigated using high-speed photography (1000 fps), combined with digital imageprocessing technology. The departure time of the bubble first decreases and then increases, and maximumof aspect ratio and specific surface area reduce as the proportion of solute increases. The bubbledeparture time decreases and bubble size increases with viscous force increases in the case of same surfacetension. Based on force balance analysis, added mass force is one of the prime reasons to affect bubbleoscillation during the acceleration phase after bubble detachment, whereas the effect of added massforce decreases as solute increases or as the bubble reaches stability zone. Also, velocity, drag coefficientand aspect ratio of bubble were discussed in detail by dimensionless analysis. Furthermore, three newshape numerical models (based on We, Ta, and WeEo, respectively) were proposed, which allowed for satisfactorypredictions, and new correlations represent well the experimental data in a wider range of Monumbers from the mentioned literature (11.6 logMo 0.63).