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RISS 인기검색어
- 검색키워드
- 저자 : Zhan Dongbo (검색결과 2 건)
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Deletion of lacD gene affected stress tolerance and virulence of Streptococcus suis serotype 2
Jiang Xiaowu,Zhu Lexin,Zhan Dongbo 한국미생물학회 2022 The journal of microbiology Vol.60 No.9
Streptococcus suis type 2 (S. suis type 2, SS2), an infectious pathogen which is zoonotic and can induce severely public health concern. Our previous research identified a newly differential secreted effector of tagatose-bisphosphate aldolase (LacD) mediated by VirD4 factor within the putative type IV secretion system of SS2, whereas the functional basis and roles in virulence of LacD remain elusive. Here in this study, the LacD was found enzymatic and can be activated to express under oxidative stress. Gene mutant and its complemental strain (ΔlacD and cΔlacD) were constructed to analyze the phenotypes, virulence and transcriptomic profiles as compared with the parental strain. The lacD gene deletion showed no effect on growth capability and cells morphology of SS2. However, reduced tolerance to oxidative and heat stress conditions, increased antimicrobial susceptibility to ciprofloxacin and kanamycin were found in ΔlacD strain. Further, the LacD deficiency led to weakened invasion and attenuated virulence since an easier phagocytosed and more prone to be cleared of SS2 in macrophages were shown in ΔlacD mutant. Distinctive transcriptional profiling in ΔlacD strain and typical downregulated genes with significant mRNA changes including alcohol dehydrogenase, GTPase, integrative and conjugative elements, and iron ABC transporters which were mainly involved in cell division, stress response, antimicrobial susceptibility and virulence regulation, were examined and confirmed by RNA sequencing and real time qPCR. In summary, the results demonstrated for the first time that LacD was a pluripotent protein mediated the metabolic, stress and virulent effect of SS2.
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Analysis of mechanical contact behavior between the catheter and trachea tissue during bronchoscopy
Yudong Bao,Shengqian Qu,Dongbo Qi,Wen Wei,Yang Zhan,Hejun Liu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.3
As for the contact mechanics between the catheter and trachea tissue during bronchoscopy, the anterior and curved part of the catheter were selected for analysis. The material parameters of each sample were obtained by uniaxial tensile experiments. And this paper verified the stress changes during the contact process using ABAQUS software from three aspects: different material property parameters, contact angle, and catheter bending angle. The results show that: when the characteristic parameter is 90°, the force on the trachea is minimal. And the force changes in a small range when the contact angle is kept at 45°-75°. When the catheter enters the next level of the trachea, the bending part is in contact with the trachea wall. At this time, keeping the bending angle at 15°-30° helps to reduce the contact force. This study could provide a theoretical basis for surgical instruments' interventional therapy.
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