Effect of MXene nanosheets attached to carbonyl iron microspheres on the performance and stability of magnetorheological fluid

Yuxi Sun,Yu Wang,Huaxia Deng,Min Sang,XingLong Gong 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

To improve the sedimentation stability of carbonyl iron (CI)-based magnetorheological fluid (MRF), anovel MRF with CI/MXene particles as dispersed phase was prepared. CI/MXene particles were preparedby attaching the lightweight MXene nanosheets onto the surface of CI particles (CIPs) as an additive. Themicro-morphology, crystal structure and element composition of CI/MXene particles were analyzed bySEM, XRD and XPS characterization respectively, which confirmed the successful synthesis of CI/MXene particles. CI/MXene-based MRF possessed considerable high magnetorheological (MR) effect,and the magneto-induced shear stress was up to 49 kPa. More importantly, the sedimentation ratio ofCI/MXene-based MRF was further characterized to evaluate its stability. Experimental results showedthat the sedimentation ratio of MRF increased from 75.7% to 98.0% after mixing CIPs with 3 wt%MXene nanosheets. The sedimentation ratio of CI/MXene-based MRF increased by 29.5% compared toCI-based MRF. As a result, the addition of MXene nanosheets significantly improved the sedimentationstability of MRF while maintained high MR effect.

라그랑지 기법을 쓴 영 공간 기반 선형 판별 분석법의 변형 기법

호우위시(Yuxi Hou),민황기(Hwang-Ki Min),송익호(Iickho Song),최명수(Myeong Soo Choi),박선(Sun Park),이성로(Seong Ro Lee) 한국통신학회 2013 韓國通信學會論文誌 Vol.38 No.2(융합기술)

선형 판별 분석에서 부류안 분산 행렬의 영 공간 재공식화

호우위시(Yuxi Hou),이원주(Wonju Lee),이승원(Seungwon Lee),안태훈(Taehun An),이선영(Sun young Lee),민황기(Hwang-Ki Min),송익호(Iickho Song) 한국통신학회 2012 한국통신학회 학술대회논문집 Vol.2012 No.11

Comparison of the Prognostic Value of Platelet-Related Indices in Biliary Tract Cancer Undergoing Surgical Resection

Lejia Sun,Yuxi Wei,Yang Chen,Wenmo Hu,Xin Ji,Haifeng Xu,Shunda Du,Haitao Zhao,Xin Lu,Xinting Sang,Shouxian Zhong,Huayu Yang,Yilei Mao 대한암학회 2021 Cancer Research and Treatment Vol.53 No.2

Purpose Platelet-related indices, including mean platelet volume (MPV) and plateletocrit (PCT), have been reported as new prognostic factors of overall survival (OS) in many cancers, but not yet in biliary tract cancer (BTC). We intended to assess these indices in predicting OS in BTC patients with the aim to build a new prognostic model for patients with BTC after surgical resection. Materials and Methods Survival analysis and time receiver operating characteristic analysis were applied to screen the platelet indices. Univariate and multivariate Cox analyses were used to identify independent prognostic factors and develop a new prognostic model. Harrell’s C-statistics, calibration curves, and decisive curve analysis were used to assess the model. Results MPV and platelet distribution width (PDW)/PCT showed the best prognostic accuracy among the platelet indices. In multivariable analysis, factors predictive of poor OS were presence of nodal involvement, Non-radical surgery, poor tumor differentiation, carbohydrate antigen 19-9 > 100 U/mL, MPV > 8.1 fl, and PDW/PCT > 190. The new model was found to be superior to the TNM staging system and our new staging system showed higher discriminative power.Conclusion MPV and PDW/PCT have high prognostic value in BTC patients, and the novel staging system based on these two indices showed good discrimination and accuracy compared with the American Joint Committee on Cancer 7th TNM staging system.

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Lingling Zhou,Yuxi Di,Mingfei Zhang,Yichang Chen,Ruonan Sun,Meiyu Shen,Fengxiang Tian,Pei Yang,Feiya Qian 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.1

Purpose: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component in Rehmanniae Radix (RR), has been found to regulate human immunity. However, the effects of CAT on Th17 cell differentiation and improvement of RA are not clear. Materials and Methods: Collagen-induced arthritis (CIA) mice were constructed to detect the effects of CAT on arthritis and Th17 cells. The effect of CAT on Th17 differentiation was evaluated with let-7g-5p transfection experiments. Flow cytometry was used to detect the proportion of Th17 cells after CAT treatment. Levels of interleukin-17 and RORγt were assessed by qRT-PCR and enzyme-linked immunosorbent assay. The expression of signal transducer and activator of transcription 3 (STAT3) was determined by qRT-PCR and Western blot. Results: We found that the proportion of Th17 cells was negatively associated with let-7g-5p expression in CIA mice. In in vitro experiments, CAT suppressed traditional differentiation of Th17 cells. Simultaneously, CAT significantly decreased Tregs-to-Th17 cells transdifferentiation. Our results demonstrated that CAT inhibited Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p and that the suppressive effect of CAT on traditional differentiation of Th17 cells is not related with let-7-5p. Conclusion: Our data indicate that CAT may be a potential modulator of Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p to reduce the expression of STAT3. These results provide new directions for research into RA treatment.

TGF-β1 induces VEGF expression in human granulosa-lutein cells: a potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

Lanlan Fang,Yiran Li,Sijia Wang,Yuxi Li,Hsun-Ming Chang,Yuyin Yi,Yang-yang Sun,Avinash Thakur,Peter C. K. Leung,Jung-Chien Cheng,Ying-Pu Sun 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Ovarian hyperstimulation syndrome (OHSS) is one of the most serious and iatrogenic complications that can occur during in vitro fertilization treatment. Although the pathogenesis of OHSS is not fully understood, vascular endothelial growth factor (VEGF) has been recognized as an important mediator of the development of OHSS. Transforming growth factor-beta-1 (TGF-β1) is known to regulate various ovarian functions. However, whether VEGF can be regulated by TGF-β1 in human granulosa cells has not been determined. In addition, the role of TGF-β1 in the pathogenesis of OHSS remains unknown. In the present study, we demonstrate that TGF-β1 stimulates VEGF expression in and secretion from both immortalized human granulosa-lutein (hGL) cells and primary hGL cells. Our results demonstrate that the SMAD2/3, ERK1/2, and p38 MAPK signaling pathways are involved in TGF-β1-induced VEGF expression and secretion. Using a mouse OHSS model, we show that the expression levels of TGF-β1 and VEGF are increased in the ovaries of OHSS mice. Blocking TGF-β1 signaling inhibits the development of OHSS by attenuating VEGF expression. Moreover, clinical results reveal that the protein levels of TGF-β1 and VEGF are increased in the follicular fluid of patients with OHSS, and that the levels of these two proteins in the follicular fluid are positively correlated. The results of this study help to elucidate the mechanisms by which VEGF expression is regulated in hGL cells, which could lead to the development of alternative therapeutic approaches for treating OHSS.

Multimode participation of traditional Chinese medicine in the treatment of COVID-19

Tieying Dai,Leyin Zhang,Xinyang Dai,Xinran Zhang,Beibei Lu,Yuxi Zheng,Deyi Shen,Yici Yan,Congqi Ji,Jieru Yu,Leitao Sun 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.-

Background The outbreak of COVID-19 has swiftly spread across China and all over the world, resulting in severe contagious pneumonia. However, no specific anti-COVID-19 drugs or methods are available for the treatment of this acute and fatal disease. In recent years, as the efficacy and safety of traditional Chinese medicine (TCM) have been universally acknowledged, it has been brought to a crucial status domestically and overseas for the treatment of COVID-19. Methods We searched relevant literature, electronic databases, and official statements, diagnoses and protocols to retrieve studies and applications related to traditional Chinese medicine for COVID-19 in terms of regulations and policies, clinical evidence, preclinical rationale and big data analysis and then summarized the discovery and development of potential drugs and their targets. Results Clinicians, researchers, governments, the public, colleges, institutes and companies collected and classified associated policies, regulations and actual contributions, searched clinical trials and preclinical experimental outcomes from databases, studied potential TCM drugs with possible mechanisms, retrieved numerous big data analysis method and gathered pooled results of compounds along with their effective targets to make traditional Chinese medicine vital to cover all stages of patients in the treatment and control of COVID-19. Conclusion Traditional Chinese medicine provides new evidence to support the clinical value of TCM for COVID-19. Background The outbreak of COVID-19 has swiftly spread across China and all over the world, resulting in severe contagious pneumonia. However, no specific anti-COVID-19 drugs or methods are available for the treatment of this acute and fatal disease. In recent years, as the efficacy and safety of traditional Chinese medicine (TCM) have been universally acknowledged, it has been brought to a crucial status domestically and overseas for the treatment of COVID-19. Methods We searched relevant literature, electronic databases, and official statements, diagnoses and protocols to retrieve studies and applications related to traditional Chinese medicine for COVID-19 in terms of regulations and policies, clinical evidence, preclinical rationale and big data analysis and then summarized the discovery and development of potential drugs and their targets. Results Clinicians, researchers, governments, the public, colleges, institutes and companies collected and classified associated policies, regulations and actual contributions, searched clinical trials and preclinical experimental outcomes from databases, studied potential TCM drugs with possible mechanisms, retrieved numerous big data analysis method and gathered pooled results of compounds along with their effective targets to make traditional Chinese medicine vital to cover all stages of patients in the treatment and control of COVID-19. Conclusion Traditional Chinese medicine provides new evidence to support the clinical value of TCM for COVID-19.

Melatonin stimulates aromatase expression and estradiol production in human granulosa-lutein cells: relevance for the high serum estradiol levels in ovarian hyperstimulation syndrome

Jung-Chien Cheng,Lanlan Fang,Yiran Li,Sijia Wang,Yuxi Li,Yang Yan,Qiongqiong Jia,Ze Wu,Zhen Wang,Xiaoyu Han,Ying-Pu Sun 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Ovarian hyperstimulation syndrome (OHSS) is one of the most life-threatening and potentially fatal complications associated with controlled ovarian hyperstimulation (COH) during in vitro fertilization (IVF) treatment. Although the pathogenesis of OHSS remains unclear, elevated serum estradiol (E2) levels before human chorionic gonadotropin (hCG) administration are associated with the risk of OHSS. The pineal hormone melatonin and its receptors are expressed in human granulosa cells and have been shown to stimulate E2 production. However, the effect of melatonin on the expression of aromatase, an enzyme responsible for a key step in the biosynthesis of E2, in human granulosa cells remains to be determined. Here, we demonstrate that melatonin upregulates aromatase expression in primary cultured human granulosa-lutein (hGL) cells through the melatonin receptor-mediated PKA-CREB pathway. Using a mouse model of OHSS, we demonstrate that administration of the melatonin receptor inhibitor luzindole inhibits the development of OHSS. In addition, the expression of ovarian aromatase and serum E2 levels are upregulated in OHSS mice compared to control mice, but this upregulation is attenuated by inhibition of the function of melatonin. Moreover, clinical results reveal that aromatase expression levels are upregulated in hGL cells from OHSS patients. Melatonin and E2 levels in the follicular fluid are significantly higher in OHSS patients than in non-OHSS patients. Furthermore, melatonin levels are positively correlated with E2 levels in follicular fluid. This study helps to elucidate the mechanisms mediating the expression of aromatase in hGL cells and provides a potential mechanism explaining the high E2 levels in patients with OHSS.

회귀 신경망에서 유한 상태 자동기계를 부호화하는 방법

원성환(Sung Hwan Won),송익호(Iickho Song),이선영(Sun-Young Lee),이승원(Seungwon Lee),민황기(Hwang-Ki Min),호우위시(Yuxi Hou) 한국통신학회 2010 한국통신학회 학술대회논문집 Vol.2010 No.6

<i>N</i>,<i>N</i>′-Diacetyl-<i>p</i>-phenylenediamine restores microglial phagocytosis and improves cognitive defects in Alzheimer’s disease transgenic mice

Park, Min Hee,Lee, Misun,Nam, Geewoo,Kim, Mingeun,Kang, Juhye,Choi, Byung Jo,Jeong, Min Seock,Park, Kang Ho,Han, Wan Hui,Tak, Eunyoung,Kim, Min Sun,Lee, Juri,Lin, Yuxi,Lee, Young-Ho,Song, Im-Sook,Choi National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.47

<P><B>Significance</B></P><P>Microglial dysfunction accompanying the loss of phagocytic ability and the overexpression of neurotoxic factors presents a positive-feedback loop that contributes to the rapid progression of neurodegeneration. Termination of this cycle is considered a promising strategy to halt the progression of neurodegenerative diseases, including Alzheimer’s disease; however, effective chemical reagents for this purpose have been very limited. Herein, we report a compact synthetic molecule capable of restoring microglial dysfunction and improving cognitive function. Our in-depth studies of such a molecular entity could be beneficial toward the urgent global search for a new and effective treatment of neurodegenerative disorders.</P><P>As a central feature of neuroinflammation, microglial dysfunction has been increasingly considered a causative factor of neurodegeneration implicating an intertwined pathology with amyloidogenic proteins. Herein, we report the smallest synthetic molecule (<I>N</I>,<I>N</I>′-diacetyl-<I>p</I>-phenylenediamine [DAPPD]), simply composed of a benzene ring with 2 acetamide groups at the <I>para</I> position, known to date as a chemical reagent that is able to promote the phagocytic aptitude of microglia and subsequently ameliorate cognitive defects. Based on our mechanistic investigations in vitro and in vivo, 1) the capability of DAPPD to restore microglial phagocytosis is responsible for diminishing the accumulation of amyloid-β (Aβ) species and significantly improving cognitive function in the brains of 2 types of Alzheimer’s disease (AD) transgenic mice, and 2) the rectification of microglial function by DAPPD is a result of its ability to suppress the expression of NLRP3 inflammasome-associated proteins through its impact on the NF-κB pathway. Overall, our in vitro and in vivo investigations on efficacies and molecular-level mechanisms demonstrate the ability of DAPPD to regulate microglial function, suppress neuroinflammation, foster cerebral Aβ clearance, and attenuate cognitive deficits in AD transgenic mouse models. Discovery of such antineuroinflammatory compounds signifies the potential in discovering effective therapeutic molecules against AD-associated neurodegeneration.</P>